Avaliação histológica e microbiológica de curativos de demora à base de hidróxido de cálcio usados em endodontia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação in vitro da associação do efeito antimicrobiano do ozônio associado a veículos e curativos de demora em diferentes períodos de tempo de armazenagem

Pós-graduação em Odontologia - FOAR

Efeito de soluções irrigadoras e do hidróxido de cálcio como curativo de demora sobre o LPS-endotoxina. Estudo histopatológico em dentes de cães

Pós-graduação em Odontologia - FOAR

Avaliação da difusão de íons hidroxila e da atividade antibacteriana de medicação intracanal á base de hidróxido de cálcio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Métodos de indução experimental de lesão periapical em dentes de cães. Análise histopatológica e histomicrobiológica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Biologia molecular - técnica PCR: monitoração do efeito de curativos de demora à base de hidróxido de cálcio na microbiota de canais radiculares em dentes de humanos portadores de lesão periapical crônica

Pós-graduação em Odontologia - FOAR

Efeito do curativo de demora à base de hidróxido de cálcio na reparação apical e periapical, pós tratamento de canais radiculares de dentes de cães com necrose pulpar e reação periapical crônica induzida. Análise histológica

Pós-graduação em Odontologia - FOAR

Effects of coronal leakage on concentration of hydrogen ions and calcium hydroxide release of several calcium hydroxide pastes over different periods of time

Purpose: To evaluate the effects of coronal leakage on concentration of hydrogen ions (pH) and calcium release of several calcium hydroxide pastes, over different periods of time. Material and Methods: Fifty extracted human mandibular central incisors (n=10) were instrumented up to the F2 instrument and assigned to the following intracanal dressing: G1- Calen, G2- Calen with 0.4% chlorhexidine (CHX), G3- Calcium hydroxide with camphorated paramonochlorophenol (CPMC) and glycerin, G4- Calen, but temporary filling material maintained during all test (positive control) and G5- Root canal without intracanal dressing (negative control). All groups were immersed in distilled water for 7 days. In sequence, the temporary filling materials were removed, except in controls groups. All specimens were individually mounted on a specific device and only its root again immersed in distilled water. Concentration of hydrogen ions and calcium release by calcium hydroxide pastes in distilled water were evaluated in 24h, 7, 14 and 28 days. The results were submitted to ANOVA test (p = 0.05). After 28 days, root canals from experimental groups were examined in SEM. Results: G1, G2, G3 and G4 presented similar pH values and calcium release and did not differ from each other (p>0.05), up to 7 days. After this time G1, G2 and G3 presented values lower values than G4 (p<0.05). In SEM analysis, calcium hydroxide residues were observed in all experimental groups. Conclusions: After 7 days, coronal leakage decreased the concentration of hydrogen ions and calcium ion release provided by all calcium hydroxide pastes.

Avaliação in vitro do pH do hidróxido de cálcio usado como medicação intracanal em associação com clorexidina e racealfatocoferol

Objective: The aim of this study was to evaluate the pH of calcium hydroxide (CalenTM) when associated or not with chlorhexidine 0.4%, and when associated with chlorhexidine with the addition of 20% or 10% of alphatocopherol (AchéTM), assessed in several periods of time. Methods: Fourty dentine tubes 20 mm, properly standardized, were made from bovine anterior teeth roots. Following, a perforation was achieved in the roots distal face at 7 mm from the cervical radicular line by using a #1/2 carbide bur. After complete root sealing is made, except in the perforation local, the radicular canals were filled with one of the following associations: Group I – Calen®; Group II – Calen™ with chlorehxidine at 0.4%; Group III – Calen™ with chlorhexidine at 0.4% with the addition of 20% (weight) of alhatocopherol compound and Group IV – Calen™ with chlorhexidine at 0.4% with the addition of 10% (weight) alphatocopherol. After cervical sealing is accomplished, the roots were immersed in water MiliQ and the pH, assessed in 24h, 7, 14, 21, 28 and 45 days. Results and Conclusion: In all periods tested, the pH of the calcium hydroxide (Calen™) was similar to the pH of the calcium hydroxide (Calen™) associated with chlorhexidine 0.4% and 10% alphatocopherol (p > 0.05). The association of 20% alphatocopherol obtained the pH lower than the association with 10% (p < 0.05). The pH of the association with chlorhexidine was similar to the pure calcium hydrocide (Calen™) after the 14th day (p > 0.05) only. Therefore, on the 45th day, this difference was significant again (p < 0.05).

Biocompatibility of Intracanal Medications Based on Calcium Hydroxide

The aim of this study was to evaluate the rat subcutaneous tissue reaction to calcium hydroxide-based intracanal medicaments, UltraCal XS (calcium hydroxide, barium sulphate, aqueous matrix), Hydropast (calcium hydroxide, barium sulphate, and propyleneglycol), and Calen (Calcium hydroxide, zinc oxide, colophony, and polyethyleneglycol), used as a control. Methods. Forty-eight rats (Rattus Norvegicus Holtzman) were distributed in three groups: Calen, UltraCal XS, and Hydropast. Polyethylene tubes filled with one of the medicaments were implanted in the dorsal subcutaneous. After 7 and 30 days, the implants were removed and the specimens were fixed and embedded in paraffin. Morphological and quantitative analyses were carried out in the HE-stained sections. The numerical density of inflammatory cells in the capsule was evaluated and statistical analyses were performed (P>/0.05). Results. At 7 days, all materials induced an inflammatory reaction in the subcutaneous tissue adjacent to the implants. In all groups, a significant reduction in the number of inflammatory cells and giant cells was verified in the period of 30 days. Conclusion. These results indicate that the calcium hydroxide-based medicaments evaluated present biocompatibility similar to Calen.

Atividade antimicrobiana residual de pastas à base de hidróxido de cálcio e associações como medicação intracanal

A resistência do biofilme endodôntico pode requerer um período maior de permanência da medicação intracanal a fim de prolongar sua ação antimicrobiana. O objetivo deste estudo foi avaliar a ação antimicrobiana residual das medicações intracanal: G1) hidróxido de cálcio + soro; G2) Calen; G3) Calen + PMCC; G4) Calen + clorexidina 0,4% e G5) clorexidina gel 2% frente à Enterococcus faecalis, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus e Kocuria rhizophila em 60 canais radiculares bovinos. Os canais radiculares foram instrumentados e preenchidos com as medicações intracanal, as quais foram removidas após os períodos experimentais (15, 30, 60 e 90 dias) e empregadas na técnica de difusão em ágar. Os halos de inibição foram evidenciados por gel de TTC e então mensurados. Os dados de halos de inibição foram submetidos à análise estatística dos testes Kruskall-Wallis e Dunn, com nível de significância de 5%. O Ca(OH)2 + soro apresentou efeito até o período de 15 dias. A pasta Calen demonstrou efeito residual aos 60 dias frente ao S. aureus, enquanto Calen/PMCC até o período de 90 dias frente à S. aureus e K. rhizophila e aos 30 dias para E. faecalis. A pasta Calen associado à clorexidina foi efetiva frente à E. faecalis até o período de 60 dias. A clorexidina gel 2% apresentou ação residual em todos os períodos, com exceção de E. faecalis aos 90 dias, também apresentou atividade antimicrobiana mais prolongada, seguido das associações do hidróxido de cálcio com clorexidina e PMCC.

Determination of the p-monochlorophenol residue in the calcium hydroxide plus p-monochlorophenol combination used as an intracanal dressing in pulpless teeth of dogs with induced chronic periapical lesion

To determine the presence of p-monochlorophenol in the calcium hydroxide (Calen) + p-monochlorophenol combination after its use as intracanal dressing, periapical lesions were induced in 60 root canals of upper and lower premolars of four dogs, After biomechanical preparation, the root canals received the intracanal medication, which was removed from the apical third after 2, 4, 7, and 14 days for chemical analysis by spectrophotometry, the results showed a p-monochlorophenol loss of approximately 50.0% in the dressing after 48 h, with no further significant loss after longer periods of times, p-Monochlorophenol was still present in the medication after 14 days.

Response of mice connective tissue to intracanal dressings containing chlorhexidine

Substances containing chlorhexidine (CHX) have been studied as intracanal medicaments. The aim of the present study was to characterize the response of mouse subcutaneous connective tissue to CHX-containing medications by conventional optical microscopy. The tissue response was evaluated by implanting polyethylene tubes containing one of the substances evaluated: Calen paste + 0.5% CHX, Calen + 2% CHX, 2% CHX gel, and Calen paste (control). After experimental periods of 7, 21, and 63 days, the implants (n = 10) were removed along with the subcutaneous connective tissue. Tissue samples were subjected to histological processing, and sections were stained with hematoxylin and eosin. Qualitative and quantitative analyses of the number of inflammatory cells, blood vessels, and vascularized areas were performed. Results were analyzed by ANOVA and Tukey tests with the significance level set at 5%. We concluded that Calen + 0.5% CHX led to reparative tissue response in contrast with Calen + 2% CHX and 2% CHX gel, which induced persistent inflammatory response, pointing to the aggressive nature of this mixture. When Calen + 2% CHX and 2% CHX gel were compared, the latter induced more intense inflammatory response. Microsc. Res. Tech., 2012. (C) 2012 Wiley Periodicals, Inc.

Plug apical com MTA em dente adjacente à área da fissura labiopalatina

O tratamento endodôntico de dentes com forame apical amplo requer consideração especial, devido ao risco de extravasamento do material obturador nos tecidos periapicais durante a obturação, de maneira relevante em áreas próximas às fissuras labiopalatinas. A criação de uma barreira apical é indicada em casos de difícil travamento do cone principal e consequente deficiência do selamento apical. O MTA em decorrência de suas excelentes propriedades biológicas, é o material de escolha para permanecer em contato com tecidos periodontais e periapicais, fazendo parte do arsenal endodôntico especificamente nestes casos de ápice aberto para a confecção de plug apical. Relato clínico: Indivíduo do gênero feminino, com fissura labiopalatina bilateral transforame, a distal dos dentes 11 e 21; compareceu no setor de endodontia do Hospital de Reabilitação de Anomalias Craniofaciais (HRAC-USP), apresentando necrose pulpar do dente 21. Na primeira sessão foi realizado o acesso cirúrgico, neutralização do conteúdo séptico/tóxico em sentido corono-apical, odontometria, biomecânica, curativo intracanal (pasta Calen) selamento provisório com cimento de ionômero de vidro. Na segunda sessão, foi realizada a obturação onde ocorreu extravasamento de material obturador, verificado através da radiografia comprobatória; justificando sua remoção e nova obturação, com prévia confecção de plug apical com MTA. Foi realizada proservação aos 6 meses pós tratamento endodôntico. Conclusão: neste caso, o plug apical com MTA possibilitou a obturação final do canal radicular com segurança, pela confecção de um anteparo apical, evitando extravasamento de material obturador e preservando a área de fissura labiopalatina.

Ad. C. Herenniu[m] Rhetorici seu de institutione oratoria lib. IIII /

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