O papel da serapilheira no desenvolvimento inicial de mudas de espécies florestais nativas

A serapilheira de matas e florestas é rica em matéria orgânica e responsável pela ciclagem de nutrientes que ocorre neste ecossistema. Além de atuar como protetora do solo e habitat para diversos microrganismos e insetos. O presente estudo buscou analisar o papel da serapilheira no desenvolvimento de mudas florestais nativas em viveiros. As espécies utilizadas no experimento foram Parapitadenia rigida (Benth) (angico-vermelho), Acacia polyphylla DC. (monjoleiro), Lonchocarpus muehlbergianus Hassl. (embira-de-sapo) e Schizolobium parahyba (Vell.)S.F.Blake (guapuruvu). O crescimento foi analisado em quatro substratos: mineral (S1), mineral com serapilheira na proporção de volume 1:1 (S2), orgânico usado em viveiros (S3) e orgânico usado em viveiros com serapilheira na proporção de volume 1:1 (S4). O delineamento experimental consistiu em blocos ao acaso com 16 tratamentos e 5 repetições. Durante uma primeira fase foram feitas as medições de altura e diâmetro das mudas em fase de viveiro. A segunda fase foi a análise da biomassa seca e avaliação da sobrevivência inicial em campo. O resultado apresentou melhores desempenhos no crescimento e sobrevivência nos tratamentos com serapilheira em comparação aos sem serapilheira. Podemos concluir que a serapilheira apresenta potenciais benéficos relevantes para a produção de mudas florestais

Ensaio de ligações de estruturas de madeira por chapas com dentes estampados em quatro espécies da família leguminosas

In the last years, the use of industrialized systems of lattice structures of wood as an alternative in the construction processes, is becoming more popular in Brazil, mainly for their commitment to the environment.The industrialized system of wooden lattice structures consists in a production of lattice structures, composed of pieces of wood where their mechanical connections are made with the sheet multi-toothed connectors (CDE).Among the many challenges to make this system competitive, the whole system of the connections between the pieces of wood not only must show functionality, but also speed, strength, versatility and economy. Referenced at Brazilian Standard for Wood Structures (NBR 7190/1997 - Project of timber structures) the sheet multi-toothed connectors, are analyzed using three test methods: tensile strength parallel to grain, tensile strength normal to the fibers and shear strength, all of them in two positions, αCH0=0o e αCH0=90o to four types of wood: Angelim (Vatairea heteroptera Ducke); Red-Angico (Parapiptadenia rigida (Benth) Brenae); Garapa (Apuleia leiocarpa (Vog.) Macbr) and Jatoba (Hymenaea stilbocarpa Hayne), belonging to the Leguminosae family and founded in several regions of Brazil.The purpose of this manuscript consists to analyze the mechanical connections with the sheet multi-toothed connectors through tests from NBR7190/1997

DEVELOPMENT OF MICROSATELLITE MARKERS FOR ANADENANTHERA COLUBRINA (LEGUMINOSAE), A NEOTROPICAL TREE SPECIES

Premise of the study: We developed and characterized nuclear microsatellite markers for Anadenanthera colubrina, a tropical tree species widely distributed in South America. Methods and Results: Leaf samples of mature A. colubrina trees, popularly called "angico," were collected from an area that is greatly impacted by agricultural practices in the region of Ribeirao Preto in Sao Paulo State in southeastern Brazil. Twenty simple sequence repeat (SSR) markers were developed, 14 of which had polymorphic loci. A total of 96 alleles were detected with an average of 6.86 alleles per polymorphic locus. The expected heterozygosity, calculated at polymorphic loci, ranged from 0.18 to 0.83. Finally, we demonstrated that 18 loci were cross-amplified in A. peregrina. Conclusions: A total of 14 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. colubrina.

Beneficial effects of Anadenanthera colubrina (Vell.) Brenan extract on the inflammatory and nociceptive responses in rodent models

Ethnopharmacological relevance:Anadenantheracolubrina (Vell.) Brenan,popularlyknownas “angico”, is a plantthathasbeenwidelyusedinfolkmedicineduetoitsanti-inflammatory property.Toevaluatethe pharmacological activitiesofthisplant,studieswereperformedonitsantinociceptiveandanti- inflammatoryproperties. Materials andmethods: The AEof Anadenantheracolubrina, madefromthebark,wasusedinrodentsvia oral route(p.o.),at100,200,and400mg/kginclassicalmodelsofnociception(aceticacid-induced writhing andhot-platetest)andinflammation evokedbycarrageenan(e.g.,pawedema,peritonitis,and synovitis). Results: The aceticacid-inducedabdominalwrithesinmiceweresignificantly reduced(Po0.001)by oral treatmentwiththeextract(100,200,and400mg/kg),buttheextractdidnotsignificantly increase the latencyinthenociceptivehot-platetest. Anadenantheracolubrina aqueousextractreduced significantly theedemaand,besides,diminishedthemieloperoxidaseactivity(200and400mg/kg, Po0.01).Thecarrageenan-inducedperitonitiswassignificantly reduced(Po0.05) bytheaqueous extractat100,200,and400mg/kg.Theaqueousextract(200mg/kg)reducesthesynovialleukocyte infiltration oncarrageenan-inducedsynovitisinrats(Po0.01),butfailedtosignificantly affectjoint swelling andimpairedmobility. Conclusions: Weshowforthe first timethattheanti-inflammatory andperipheralantinociceptive activities of Anadenantheracolubrina are consistent,atleastinpart,withtheuseofthisplantinpopular medicine practices.

Vicilina de sementes da leguminosa selvagem Anadenanthera macrocarpa: purificação, caracterização, efeito deletério e mecanismo de ação para Callosobruchus maculatus

Grains and legume seeds are foods that form the basis of the diets of many cultures around the world, winch contritbute to the daily nutrient requirements of humans. Vicilins (7S globulin) are storage proteins found in legume seeds, and may have an additional function constitutive defense of the embryo against pests and pathogens. In this work the vicilin from Anadenanthera macrocarpa - AmV (red-angico), was purified and partially characterized, its effect on development and larval survival and adult emergence of Callosobruchus maculatus was evaluated by determination of LD50, WD50 and ED50 in system bioassay. Purification of vicilin was initiated by the chitin affinity chromatography and then gel filtration (Superdex 75 Tricorn 10x300 mm) FPLC system followed by reverse phase chromatography (C8 phenomenex) on HPLC system. Bioassays WD50 and LD50 for larvae were 0.32% and 0.33% (w:w) respectively, since the ED50 for adults was 0.096%. The probable mechanism of action was evaluated by testing digestibility of AmV in vitro, and observed for the involvement of two fragments vicilins immunoreactive against polyclonal Anti-vicilin from Erythrina velutina (Anti-EvV) about of 22 and 13 kDa chitin binding. The AmV in its native form has been recognized by the anti-EvV, indicating that there is a conserved region in the vicilin and is probably corresponding to the chitin binding domains. These results point to a new vicilin chitin binding that can subsequently be used as a possible biopesticide protein source, in order to control insect pest C. maculatus and confirm literature findings that demonstrate vicilin in the presence of different kinds of ligands to conserved regions chitin not yet characterized

Growth of native trees in two agroforestry systems.

Agroforestry systems with eucalyptus prevail in Central and Southeast Brazil, and little information is available about systems using native trees. The aim of the present study was to evaluate the development of seven native tree species grown under two agroforestry systems. The experiment was conducted starting in 2007 in 12-hectare area in the municipality of São Carlos, São Paulo state, Brazil. The tree species planted in the two systems (a silvopastoral system and an agrisilvicultural system) were: 'capixingui' (Croton floribundus) and 'mutambo' (Guazuma ulmifolia) (tutors), 'jequitibá-branco' (Cariniana estrellensis), 'canafistula' (Peltophorum dubium) and 'ipê felpudo' (Zeyheria tuberculosa) (timber trees), and 'angico-branco' (Anadenanthera colubrina) and 'pau-jacaré' (Piptadenia gonoacantha) (N-fixing trees). Data were collected for 48 months. The results show differences among tree development, which was evaluated as growth in height and diameter, as well as sensitivity to insect and disease damage. The overall results show that the agrisilvicultural system allowed better tree development. The species with best performance in the two systems were capixingui, mutambo and canafístula. Ipê-felpudo and jequitibá-branco showed the worst results. The high variability among individuals of the same species indicates the possibility of high production advances with selective breeding of these species.