Entrepreneurship in a Hub-and-Spoke Industrial District: Firm Survey Evidence from Seattle's Technology Industry

Mayer H. Entrepreneurship in a hub-and-spoke industrial district: firm survey evidence from Seattle's technology industry, Regional Studies. The paper investigates entrepreneurial dynamics in a hub-and-spoke industrial district. Using data on the genealogy of high-technology firms in Seattle, Washington State, the study examines the ways in which entrepreneurial firms relate to their parent firms and the role of agglomeration economies. The results illustrate that entrepreneurship is an important vehicle for the diversification of such a district. When compared, hub-related spinoffs such as those founded by former Microsoft employees do not differ much from other start-ups. The differences between Microsoft spinoffs and start-ups are very limited; both diversify the regional economy by entering new markets when compared with their parents.

An initiative for participatory monitoring of large scale acquisitions: Opportunities and results thus far

Increasing commercial pressures on land are provoking fundamental and far-reaching changes in the relationships between people and land. Much knowledge on land-oriented investments projects currently comes from the media. Although this provides a good starting point, lack of transparency and rapidly changing contexts mean that this is often unreliable. The International Land Coalition, in partnership with Oxfam Novib, Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), University of Pretoria, Centre for Development and Environment of the University of Bern (CDE), and GIZ, started to compile an inventory of land-related investments. This project aims to better understand the extent, trends and impacts of land-related investments by supporting an ongoing and systematic stocktaking exercise of the various investment projects currently taking place worldwide. It involves a large number of organizations and individuals working in areas where land transactions are being made, and able to provide details of such investments. The project monitors land transactions in rural areas that imply a transformation of land use rights from communities and smallholders to commercial use, and are made both by domestic and foreign investors (private actors, governments, government-back private investors). The focus is on investments for food or agrofuel production, timber extraction, carbon trading, mineral extraction, conservation and tourism. A novel way of using ITC to document land acquisitions in a spatially explicit way and by using an approach called “crowdsourcing” is being developed. This approach will allow actors to share information and knowledge directly and at any time on a public platform, where it will be scrutinized in terms of reliability and cross checked with other sources. Up to now, over 1200 deals have been recorded across 96 countries. Details of such transactions have been classified in a matrix and distributed to over 350 contacts worldwide for verification. The verified information has been geo-referenced and represented in two global maps. This is an open database enabling a continued monitoring exercise and the improvement of data accuracy. More information will be released over time. The opportunities arise from overcoming constraints by incomplete information by proposing a new way of collecting, enhancing and sharing information and knowledge in a more democratic and transparent manner. The intention is to develop interactive knowledge platform where any interested person can share and access information on land deals, their link to involved stakeholders, and their embedding into a geographical context. By making use of new ICT technologies that are more and more in the reach of local stakeholders, as well as open access and web-based spatial information systems, it will become possible to create a dynamic database containing spatial explicit data. Feeding in data by a large number of stakeholders, increasingly also by means of new mobile ITC technologies, will open up new opportunities to analyse, monitor and assess highly dynamic trends of land acquisition and rural transformation.

Editorial: Roles and Expertise

We all take on roles, probably several each day. Parent, worker, consumer, spouse, or shortstop, the roles we play are varied and complex. After one's own family, perhaps the roles of consumer and worker are most important to Family Preservation. How do we come to play these roles, and in what ways are they changing, or should they change? Often, neither the worker or family set out to play their roles, but through the twist and turns of life, the opportunity to serve and preserve a family presents itself. At a recent conference, a group of workers spoke of how, rather than having a career goal to do Family Preservation, Family Preservation found them. Many of the families probably say the same thing! In the fields of mental health, developmental disabilities, and adoption, families may seek Family Preservation services; rarely do families involved in juvenile justice, corrections, or child welfare systems look for Family Preservation. Family Preservation finds them. And thus the roles begin.

Teoría y práctica de la restauración de la Mezquita-Catedral de Córdoba durante el siglo XX

La Mezquita-Catedral de Córdoba es un edificio vivo. Un edificio que ha sido transformado sucesivamente por hombres de razas, culturas y religiones distintas durante sus más de 1.200 años de vida y que, a pesar de ello, no ha dejado de estar en uso ni uno solo de esos días de esa larga vida. De esta forma, el edificio se muestra ante el visitante como un complejo objeto arquitectónico, resultado de una continua transformación. La capacidad de la transformación de los edificios es algo inherente a su propia condición arquitectónica, no es un hecho exclusivo de la Mezquita-Catedral. Sin embargo, en este edificio esa transformación se produce con una gran intensidad y sin pérdida de su autenticidad. Tradicionalmente, los edificios se han adaptado a los nuevos requerimientos de cada época en un proceso que ha buscado en el propio edificio las leyes o principios que habían de regir la intervención. De esta forma, tanto las sucesivas ampliaciones de la Mezquita de Abd al-Rahman I como las siguientes intervenciones cristianas debieron asumir lo preexistente como material de trabajo. Así, los arquitectos del califa al-Hakam II dialogaron con sus antecesores complejizando el espacio que recibieron, así como los Hernán Ruiz consiguieron un nuevo organismo resultante de la introducción de su arquitectura luminosa en la trama hispanomusulmana. El siglo XIX confirmó el deseo por descubrir las huellas de un pasado esplendoroso que la intervención barroca había silenciado bajo un tratamiento homogéneo del espacio. La recuperación de esas huellas supuso, hace exactamente dos siglos, el inicio de la última gran etapa en la transformación del edificio, la de la restauración. La fábrica es considerada como objeto a conservar y los esfuerzos desde ese momento se centraron en la recuperación de la arquitectura omeya latente. De este modo, la práctica de la restauración como disciplina se encontró absolutamente influenciada por la Arqueología como única fuente de conocimiento. Las intervenciones buscaban lo original como modo de recuperar espacial y formalmente aquel pasado, concentrándose en los lugares del edificio considerados como esenciales. La declaración del edificio como monumento nacional en 1882 propició que el Estado se hiciera cargo de su mantenimiento y conservación, sustituyendo en esa tarea a los Obispos y Cabildos del siglo XIX, que tuvieron un entendimiento muy avanzado para su época. La llegada del arquitecto Velázquez Bosco en las últimas décadas del siglo XIX supuso un cambio trascendental en la historia del edificio, puesto que recibió un edificio con importantes deterioros y consiguió poner las bases del edificio que hoy contemplamos. El empeño por la recuperación material y espacial devolvió a la Mezquita-Catedral buena parte de su imagen original, reproduciendo con exactitud los modelos hallados en las exploraciones arqueológicas. La llegada de Antonio Flórez tras la muerte de Velázquez Bosco supuso la traslación al edificio del debate disciplinar que se desarrolló en las dos primeras décadas del siglo XX. Flórez procuró un nuevo entendimiento de la intervención, considerando la conservación como actuación prioritaria. En 1926 el Estado reformó la manera en que se atendía al patrimonio con la creación de un sistema de zonas y unos arquitectos a cargo de ellas. La existencia de un nuevo marco legislativo apuntaló esa nueva visión conservativa, avalada por la Carta de Atenas de 1931. Este modelo restauración científica huía de la intervención en estilo y valoraba la necesidad de intervenir de la manera más escueta posible y con un lenguaje diferenciado, basándose en los datos que ofrecía la Arqueología. Por tanto, se continuaba con la valoración del edificio como documento histórico, buscando en este caso una imagen diferenciada de la intervención frente a la actitud mimética de Velázquez. Resulta destacable la manera en la que el historiador Manuel Gómez-Moreno influyó en varias generaciones de arquitectos, arqueólogos e historiadores, tanto en el entendimiento científico de la restauración como en la propia estructura administrativa. La labor desarrollada en el edificio por José Mª Rodríguez Cano primero y Félix Hernández a continuación estuvo influida de manera teórica por el método de Gómez-Moreno, aunque en muchos aspectos su labor no representó una gran diferencia con lo hecho por Velázquez Bosco. La búsqueda de lo original volvió a ser recurrente, pero la carga económica del mantenimiento de un edificio tan extenso conllevó la no realización de muchos de los proyectos más ambiciosos. Esta obsesiva búsqueda de la imagen original del edificio tuvo su última y anacrónica etapa con la intervención de la Dirección General de Arquitectura en los 70. Sin embargo, el agotamiento del modelo científico ya había propiciado un nuevo escenario a nivel europeo, que cristalizó en la Carta de Venecia de 1964 y en una nueva definición del objeto a preservar, más allá del valor como documento histórico. Esta nueva posición teórica tuvo su traslación al modelo restaurador español en el último cuarto de siglo XX, coincidiendo con la Transición. El arquitecto Dionisio Hernández Gil defendió una interpretación distinta a la de los arqueólogos y de los historiadores, que había prevalecido durante todo el siglo. En opinión de Hernández Gil, los problemas de intervención debían enfocarse fundamentalmente como problemas de Arquitectura, abandonando la idea de que solamente podían ser resueltos por especialistas. Esta convicción teórica fue defendida desde la nueva Administración y deparó la utilización de unos criterios de intervención particularizados, provenientes del análisis multifocal de cada situación y no sólo desde el valor de los edificios como documentos históricos. Y este cambio tuvo su traslación a la Mezquita-Catedral con la práctica de Gabriel Ruiz Cabrero y Gabriel Rebollo. En consecuencia con esa nueva perspectiva, aceptaron el edificio que recibieron, sustituyendo la búsqueda de aquella página original por la aceptación de cada una de las páginas de su historia y el respeto a las técnicas constructivas del pasado. La búsqueda de soluciones específicas desde el propio objeto arquitectónico significó la renovada atención a la potente estructura formal-constructiva como origen de toda reflexión. Considerar la Mezquita-Catedral en primer lugar como Arquitectura implicaba la atención a todo tipo de factores además de los históricos, como medio para preservar su autenticidad. Esta tesis pretende demostrar que la práctica de la restauración realizada en la Mezquita-Catedral a lo largo del siglo XX ha evolucionado desde la búsqueda de lo original hasta la búsqueda de lo auténtico, como reflejo de una visión basada en lo arqueológico frente a una renovada visión arquitectónica más completa, que incluye a la anterior. La consideración de la intervención en este edificio como otra página más de su historia y no como la última, significa la reedición de un mecanismo recurrente en la vida del edificio y un nuevo impulso en ese proceso de continua transformación. ABSTRACT The Mosque-Cathedral of Cordoba is a living building. A building transformed by men of different races, cultures and religions during more than 1.200 years old and that, nevertheless, it has continued to be in use all days in that long life. Thus, the building shows to the visitor as a complex architectural object, the result of continuous transformation. This transformation capacity of the buildings is inherent in their own architectural condition, it’s not an exclusive fact of the Mosque-Cathedral. However, in this building that transformation happens with a great intensity, without losing their authenticity. Traditionally, buildings have been adapted to the new requirements of times in a process that looked for laws or principles in order to guide the intervention. Thus, both the successive enlargements of the Mosque of Abd al-Rahman and Christian interventions must assume the preexistence as a working material. So, the architects of the caliph al-Hakam II spoke to their predecessors, complexing the receiving space, as well as Hernan Ruiz got a new organism as result the introduction of his luminous architecture into hispanic-muslim weft. The nineteenth century confirmed the desire to discover the traces of a glorious past that Baroque intervention had silenced, under a uniform space treatment. Exactly two centuries ago, the recovery of these traces meant the start of the last major phase in the transformation of the building: the restoration. The building was considered subject to conserve and since then, efforts focused on the recovery of latent Umayyad architecture. Thus, the practice of restoration as a discipline was absolutely influenced by Archaeology as the only source of knowledge. Interventions were seeking the original as the way to recover that past in a space and formal way, concentrating on essential sites of the building. The statement as a national monument in 1882 prompted the State take charge of its maintenance and preservation, replacing to the nineteenth century Bishops and Cabildos, which had a very advanced understanding for that time. The arrival of the architect Velazquez Bosco in the last decades of the nineteenth century involved a momentous change in the history of the building, since he received a building with significant damage and he achieved the foundations of the building that we can see today. Efforts to a material and space recover returned the Mosque-Cathedral to its original image, accurately reproducing the models found in archaeological explorations. The arrival of Antonio Florez after Velazquez’s death involved the translation of discipline debate, which was developed in the first two decades of the twentieth century. Florez tried a new understanding of the intervention, considering conservation as a priority action. In 1926, the State reformed the way in which heritage was attended, creating a zones system with a few architects in charge of them. The existence of a new legislative framework, underpinned this new conservative vision, supported by the Athens Charter of 1931. This scientific restoration model fleeing from intervention in style and it appreciated the need to intervene in the most concise way, with a distinct language based on the data offered by Archaeology. Therefore, it continued with the appraisement of the building as a historical document, seeking in this case a differentiated image of intervention, against Velazquez mimetic attitude. It is remarkable the way in which the historian Manuel Gomez-Moreno influenced several generations of architects, archaeologists and historians, both in the scientific understanding of the restoration and the administrative structure. The work of Jose Maria Rodriguez Cano first and then Felix Hernandez was theoretically influenced by the Gomez-Moreno’s method, although in many respects their work did not represent a great difference to Velazquez Bosco. The search of the original returned to recur, but the economic charge of maintaining such a large building led to the non-realization of many of the most ambitious projects. This obsessive search for the original image of the building had its last and anachronistic stage with the intervention of the Department of Architecture at 70’s. However, the exhaustion of the scientific model had already led to a new scenario at European level, which crystallized in the Venice Charter of 1964 and a new definition of the object to be preserved beyond the value as a historical document. This new theoretical position had its translation to Spanish restaurateur model in the last quarter of the twentieth century, coinciding with the Transition. The architect Dionisio Hernandez Gil defended a different interpretation from archaeologists and historians, that had prevailed throughout the century. According to Hernandez Gil, the problems of intervention should focus primarily as architectural issues, abandoning the idea that they could only be determined by specialist. This theoretical conviction was defended from the new administration and led to the use of particularized criteria, from a multifocal analysis of each situation. And this change had its translation to the Mosque with the practice of Gabriel Ruiz Cabrero and Gabriel Rebollo. Consistent with this new perspective, they accepted the receiving building, replacing the search on original page for acceptance of all historical pages and respecting the constructive techniques of the past. The search for specific solutions from the architectural object meant the renewed attention to the powerful formal-constructive structure as the origin of all thought. Consider the Mosque-Cathedral as Architecture, involved the attention to all kinds of factors in addition to the historical, as a means to preserve its authenticity. This thesis aims to demonstrate that the practice of restoration in the Mosque-Cathedral throughout the twentieth century has evolved from the search of the original to the search for the authentic, reflecting a vision based on the archaeological against a renewed more complete architectural vision, including the above. Consideration of intervention in this building as another page in its history and not the last one, means the reissue of an own mechanism and a new impetus in that continuous transformation process.

Slow cycling of unphosphorylated myosin is inhibited by calponin, thus keeping smooth muscle relaxed

A key unanswered question in smooth muscle biology is whether phosphorylation of the myosin regulatory light chain (RLC) is sufficient for regulation of contraction, or if thin-filament-based regulatory systems also contribute to this process. To address this issue, the endogenous RLC was extracted from single smooth muscle cells and replaced with either a thiophosphorylated RLC or a mutant RLC (T18A/S19A) that cannot be phosphorylated by myosin light chain kinase. The actin-binding protein calponin was also extracted. Following photolysis of caged ATP, cells without calponin that contained a nonphosphorylatable RLC shortened at 30% of the velocity and produced 65% of the isometric force of cells reconstituted with the thiophosphorylated RLC. The contraction of cells reconstituted with nonphosphorylatable RLC was, however, specifically suppressed in cells that contained calponin. These results indicate that calponin is required to maintain cells in a relaxed state, and that in the absence of this inhibition, dephosphorylated cross-bridges can slowly cycle and generate force. These findings thus provide a possible framework for understanding the development of latch contraction, a widely studied but poorly understood feature of smooth muscle.

Molecular Cloning and Characterization of a Radial Spoke Head Protein of Sea Urchin Sperm Axonemes: Involvement of the Protein in the Regulation of Sperm Motility

Monoclonal antibodies raised against axonemal proteins of sea urchin spermatozoa have been used to study regulatory mechanisms involved in flagellar motility. Here, we report that one of these antibodies, monoclonal antibody D-316, has an unusual perturbating effect on the motility of sea urchin sperm models; it does not affect the beat frequency, the amplitude of beating or the percentage of motile sperm models, but instead promotes a marked transformation of the flagellar beating pattern which changes from a two-dimensional to a three-dimensional type of movement. On immunoblots of axonemal proteins separated by SDS-PAGE, D-316 recognized a single polypeptide of 90 kDa. This protein was purified following its extraction by exposure of axonemes to a brief heat treatment at 40°C. The protein copurified and coimmunoprecipitated with proteins of 43 and 34 kDa, suggesting that it exists as a complex in its native form. Using D-316 as a probe, a full-length cDNA clone encoding the 90-kDa protein was obtained from a sea urchin cDNA library. The sequence predicts a highly acidic (pI = 4.0) protein of 552 amino acids with a mass of 62,720 Da (p63). Comparison with protein sequences in databases indicated that the protein is related to radial spoke proteins 4 and 6 (RSP4 and RSP6) of Chlamydomonas reinhardtii, which share 37% and 25% similarity, respectively, with p63. However, the sea urchin protein possesses structural features distinct from RSP4 and RSP6, such as the presence of three major acidic stretches which contains 25, 17, and 12 aspartate and glutamate residues of 34-, 22-, and 14-amino acid long stretches, respectively, that are predicted to form α-helical coiled-coil secondary structures. These results suggest a major role for p63 in the maintenance of a planar form of sperm flagellar beating and provide new tools to study the function of radial spoke heads in more evolved species.

Eine Messe des Lebens : für Soli Chor und grosses Orchester, nach Nietzsches Zarathustra /

"In die Universal Edition aufgenommen."

Also sprach Zarathustra : Tondichtung, frei nach Friedr. Nietzsche, für grosses Orchester, Op. 30 /

Originally for orchestra; arr. for 2 pianos, 4 hands.

Thus to revisit; some reminiscences of Ford Madox Hueffer.

pt. I. Before moonrise.--pt. II. Prosateurs: Credentials. Puiser dans le vide. The lordly treasure-house. Mots justes. Combien je regrette. Coda. W. H. Hudson and the simple word. Mr. Joseph Conrad and Anglo-Saxondom. Henry James, Stephen Crane and the main stream.--pt. III. The battle of the poets: "Thoughts before battle." Mr. Pound, Mr. Flint, some imagistes or cubistes, and the poetic vernacular. A descriptive interlude. "Vers libre." Second coda. Index.

I. An exposition of the symbolic terms of the second part of Faust. II. How this part thus proves itself to be a dramatic treatment of the modern history of Germany worthy of the genius of Goethe and the life he devoted to the task. (The prologues and first act completely explained; the other acts generally).

Mode of access: Internet.

Also sprach Zarathustra; ein buch für alle und keinen.

Mode of access: Internet.

Speech of Mr. Holmes, of Maine, in the Senate of the United States, on his resolutions calling upon the President of the United States for the reasons of his removing from office and filling the vacancies thus created, in the recess of the Senate.

Mode of access: Internet.