Population dynamics of the anchoveta, Cetengraulis mysticetus, in the Gulf of Panama, as determined by tagging experiments

ENGLISH: The anchoveta is the major constituent of the important bait and reduction fisheries of the Gulf of Panama. It is a short-lived species, the great majority of the catch consisting of fish in their first year of life. Fish for reduction are caught chiefly in the Isla Verde area, between Punta Chame and the entrance of the Panama Canal. In 1960 and 1961 anchovetas were tagged with metal internal tags and released in the major areas of occurrence of this species. The tags were recovered from the meal in the reduction plants with magnets. From the 53,380 fish tagged in 1960, 745 tags were returned during the 1960 season, 246 during the 1961 season, and 8 during the 1962 season. From the 113,202 tagged in 1961, 373 tags were returned during the 1961 season and 48 during the 1962 season. Complete catch statistics are available, and length-frequency and length-weight data were used to convert these from pounds to numbers of fish of each year class. The annual survival rate for the fish of the 1959 year class in the Isla Verde area was estimated to be 0.086 by the Chapman-Robson method, 0.102 by the year-class method, and 0.088 by the Jackson positive method. The first method is considered to give the best estimate. Six estimates of the population of fish of the 1959 year class in the Isla Verde area were obtained from the sample tag ratios of six experiments conducted in that area in 1960. The estimates differed due to the temporal decrease in the population, but the downward trend corresponded fairly well to what was expected from the total annual mortality rate. It was estimated that the population of 1959-year class fish was about 818 million on March 8, 1960, and about 70 million on March 8, 1961. As the population of anchovetas decreases during the season the effort increases sufficiently that the catch remains roughly constant. This is described as the "constant absolute catch" type fishery. Of the original population of fish in the Isla Verde area at the beginning of the 1960 season, about 11 per cent were caught and 81 per cent died of natural causes. Evaluation of growth and mortality data demonstrated that beginning the fishery for the youngest age group later than March 8 (the date it began in 1960) would reduce the yield per recruit, while increasing the fishing effort would greatly increase it. Further, it is believed unlikely that increases in the catch in the Isla Verde area alone would noticeably decrease the number of recruits to that area. Therefore there is no foreseeable need for regulation of the fishery. SPANISH: El principal constituyente de la importante pesquería para carnada y para reducción en el Golfo de Panamá es la anchoveta. Es una especie de vida corta cuya pesca, en su mayor parte, está constituida por peces que se encuentran en su primer año de vida. Para la industria de reducción los peces son capturados principalmente en el área de Isla Verde, entre Punta Chame y la entrada del Canal de Panamá. En 1960 y 1961 las anchovetas fueron marcadas con marcas metálicas internas y liberadas en las áreas más importantes en que se encuentra esta especie. Las marcas fueron recobradas de la harina en las plantas de reducción por medio de magnetos. De los 53,380 peces marcados en 1960, fueron devueltas 745 marcas durante la temporada pesquera de 1960, 246 durante la de 1961, y 8 durante la de 1962. De los 113,202 marcados en 1961, 373 marcas fueron devueltas durante la temporada pesquera de 1961 y 48 durante la de 1962. Se dispone de estadísticas completas de captura, y los datos de frecuencia-longitud y de longitud-peso fueron usados para convertir éstos de libras a números de peces de cada clase anual. La tasa anual de supervivencia correspondiente a la clase anual de 1959 en el área de Isla Verde estimó en 0.086 por medio del método Chapman-Robson; en 0.102 por método de la clase anual; y en 0.088 por el método positivo de Jackson. Se considera que el primer método dé la mejor estimación. Seis estimaciones de la población de peces de la clase anual 1959 en el área de Isla Verde fueron obtenidas según la proporción de marcas halladas en las muestras correspondientes a seis experimentos efectuados en aquella área en 1960. Las estimaciones variaron debido a la disminución temporal de la población, pero esta tendencia descendente correspondió bastante bien a lo que se esperaba según la tasa total de mortalidad anual. Se estimó que la población de peces de la clase anual de 1959 era de unos 818 millones el 8 de marzo de 1960, y aproximadamente de unos 70 millones el 8 de marzo de 1961. Conforme a que la población de anchovetas disminuye durante la temporada pesquera, el esfuerzo aumenta lo suficientemente como para que la pesca se mantenga más o menos constante. Este es el tipo de pesquería descrito como de "captura absoluta constante". De la población original de peces en el área de Isla Verde al comienzo de la temporada pesquera de 1960, cerca del 11 por ciento fue capturada y el 81 por ciento murió por causas naturales. La evaluación de los datos del crecimiento mortalidad demostraron que al comenzar la pesquería a explotar grupo de edad más joven en una fecha posterior al 8 de marzo (la fecha en que comenzó en 1960) se reduciría el rendimiento por recluta, mientras que al aumentar el esfuerzo de pesca lo aumentaría considerablemente. Más aún, se cree improbable que el aumento en la pesca en el área de Isla Verde de por sí disminuyera perceptiblemente el número de reclutas en esa área. En consecuencia no se prevé la necesidad de una reglamentación de la pesquería. (PDF contains 172 pages.)

Estimates of the rates of mortality of yellowfin tuna in the Eastern Pacific Ocean derived from tagging experiments

ENGLISH: Tag release and return data for the Baja California and Gulf of Guayaquil areas were selected for this study because substantial numbers of returns resulted from these releases and because the effects of emigration are small in these areas. The returns of tags per unit of fishing effort for several experiments in each area were used to estimate the coefficients of total mortality and shedding. The coefficient of annual natural mortality was estimated to be less than 2.0, which is in agreement with a previous estimate of 0.8, but does not improve upon it. The estimates for the average coefficients of catchability are 2.02 X 10-3 for the Baja California area and 0.67 X 10-3 for the Gulf of Guayaquil area. SPANISH: Se seleccionaron para este estudio algunos da tos de liberación y retorno de marcas en las áreas de Baja California y el Golfo de Guayaquil debido a que cantidades substanciales de retornos resultaron de estas liberaciones y porque los efectos de migración son pequeños en estas áreas. Los retornos de marcas por unidad de esfuerzo de pesca de varios experimentos en cada área fueron empleados para estimar los coeficientes de mortalidad total y desprendimiento. Se estimó que el coeficiente de mortalidad natural anual fue inferior a 2.0, lo que está de acuerdo con una estimación anterior de 0.8, pero no la mejora. Las estimaciones de los coeficientes promedios de capturabilidad son 2.02 X 10-3 en el área de Baja California y 0.67 X 10-3 en el área del Golfo de Guayaquil. (PDF contains 58 pages.)

Materials and methods for tagging purse seine and baitboat caught tunas

ENGLISH: The percentages of return of purse seine-tagged yellowfin and baitboat- tagged yellowfin and skipjack subjected to various combinations of treatments were tested statistically to determine the effects of the treatments. For yellowfin the precentages of return differed among sets or dates of release, and were higher for double-tagged fish, larger fish, and fish released soonest during each set. For skipjack the percentages of return differed among dates of release, and were higher for single-tagged fish and fish tagged in plastic-covered cradles. SPANISH: Los porcentajes de retorno de aleta amarilla marcado en barcos cerqueros y los de aleta amarilla y barrilete marcados en los de carnada sometidos a varias combinaciones de tratamientos fueron probados estadísticamente para determinar los efectos de los tratamientos. Con respecto al aleta amarilla los porcentajes de retorno fueron diferentes entre los lances o fechas de liberación, y fueron más altos para los peces con doble marca, peces de más talla y peces liberados lo más pronto durante cada lance. Los porcentajes de retorno del barrilete fueron diferentes entre las fechas de liberación, y fueron más altos para peces con una sola marca y peces marcados en cunas cubiertas con plástico. (PDF contains 41 pages.)

Migrations of yellowfin and skipjack tuna in the Eastern Pacific Ocean as determined by tagging experiments, 1952-1964

ENGLISH: Totals of 59,547 tagged yellowfin and 90,412 tagged skipjack were released during 1952-1964 throughout the range of the fishery in the eastern Pacific Ocean during that period. Most of the fish were released from commercial baitboats, either on regular fishing trips or on chartered trips to catch fish for tagging. There we re 8,397 yellowfin and 4,381 skipjack returned from these releases. There appear to be two main groups of yellowfin in the eastern Pacific Ocean. There is considerable intermingling among the fish of the two groups, however. The fish of the northern group (west coast of Baja California, Gulf of California, and Revillagigedo Islands) first appear in the Revillagigedo Islands in about April, and migrate north along the Baja California coast during the spring and summer and south along that coast during the fall. Recruits to the southern group (Tres Marias Islands to northern Chile) appear at many points or continuously along most of the coast. The fish which first appear in the northern Panama Bight in April migrate rapidly northwest to Central America and Mexico and south to the Gulf of Guayaquil. There also appear to be two main groups of skipjack in the eastern Pacific Ocean. The fish of the northern group (west coast of Baja California, Gulf of California, and Revillagigedo Islands ) perform about the same migration as do the yellowfin of the same area, but most of the skipjack apparently then migrate to the central Pacific Ocean during the fall and/or winter. Recruits to the southern group (Central America to northern Chile) appear mostly in or near the Panama Bight. The fish which first appear in the northern Panama Bight in April migrate rapidly northwest to Central America and south to the Gulf of Guayaquil. The proportions which migrate in these directions vary considerably from year to year, this perhaps being dependent on differences in the sea-surface temperatures. SPANISH: Durante el período de 1952-1964 se liberó a través de todos los límites de distribución de la pesquería en el Océano Pacífico oriental un total de 59,547 aleta amarilla y 90,412 barriletes marcados. La mayoria de los peces fueron liberados de barcos de carnada comerciales, o en viajes regulares de pesca o en viajes en los que se fletaron los barcos para capturar atunes y marcarlos. De estas líberaciones se recapturaron 8,397 aleta amarilla y 4,381 barriletes. Parece que haya dos grupos principales de aleta amarilla en el Océano Pacífico oriental. Sin embargo, existe una entremezcla considerable entre los peces de los dos grupos. Los peces del grupo septentrional (costa occidental de Baja California, Golfo de California y Islas Revillagigedo) aparecen primero en las Islas Revillagigedo alrededor de abril, y durante la primavera y el verano se desplazan al norte a lo largo de la costa de Baja California y durante el otoño al sur a lo largo de la costa. Los reclutas del grupo meridional (Islas Tres Marias hasta el norte de Chile) aparecen en muchas partes o continuamente a lo largo de la mayoría de la costa. Los peces que aparecen primero en la región septentrional del Panamá Bight en abril se desplazan rápidamente al noroeste a la América Central y México y al sur al Golfo de Guayaquil. Parece también que existen dos grupos principales de barrilete en el Océano Pacífico oriental. Los peces del gr upo septentrional (costa occidental de Baja California, Golfo de California e Islas Revillagigedo ) realizan casi la misma migración que el atún aleta amarilla de la misma área, pero aparentemente la mayor parte del barrilete se desplaza luego al Océano Pacífico central durante el otoño y/o en el invierno. Los reclutas al grupo meridional (América Central al norte de Chile) aparecen en su mayoría en el Panamá Bight o cerca a este lugar. Los peces que aparecen primero en la región septentrional del Panamá Bight en abril se desplazan rápidamente al noroeste a la América Central y al sur al Golfo de Guayaquil. Las proporciones que se desplazan en estas direcciones varían considerablemente de año a año; tal vez esto depende en las diferencias de temperatura de la superficie del mar. (PDF contains 227 pages.)

Migrations of yellowfin tuna in the Eastern Pacific Ocean as determined from tagging experiments initiated during 1968-1974

ENGLISH: Data from tagging experiments initiated during 1968-1974 in the eastern Pacific Ocean were used to study the migrations of yellowfin tuna in that area. The map method, the parallel-area method, and the Jones method were employed in the analyses. The map method gives a useful impression of the distances and directions traveled, but does not express these parameters in quantitative terms. The parallel-area method is particularly useful for determining whether or not there is net movement in particular directions, i.e. inshore-offshore, east-west, or north-south. The first of these is of particular interest, as the incidence of smaller fish is much higher in the catches made inshore than in those made offshore, and it is desirable to know whether this is due to relatively greater abundance or to relatively greater vulnerability of the smaller fish in the inshore areas. If the former were the case an offshore movement of the fish as they grew older would probably be detected. Such a movement was not detected, however, so it appears likely that the differences in the catches of smaller fish in the inshore and offshore areas are due mainly to differences in vulnerability. Few or no east-west or north-south tendencies in the movements of the fish were detected. The Jones method indicates that the movement is not random, but reveals no pronounced directional tendencies. SPANISH: Se emplearon los datos de los experimentos de marcado, iniciados en el Océano Pacífico oriental durante 1968-1974 para estudiar los desplazamientos del atún aleta amarilla en esa zona. En los análisis se emplearon los métodos cartográficos, de las zonas paralelas y de Jones. El método cartográfico ofrece una idea útil sobre la distancia y dirección de los desplazamientos, pero no expresa estos parámetros en términos cuantitativos. El método de las zonas paralelas es particularmente conveniente para determinar si existe o nó un desplazamiento neto en una dirección especial, es decir, hacia la costa-fuera de la costa, este-oeste o norte-sur. El primero de éstos tiene un interés especial, ya que la incidencia de peces más pequeños es muy superior en las capturas realizadas cerca de la costa que en las de mar afuera, y se desea conocer si ésto se debe a la abundancia relativamente superior o a las vulnerabilidad relativamente mayor de los pequeños peces en las zonas costeras. Si el caso fuera el primero, se podría descubrir probablemente un movimiento de los peces mar afuera a medida que crecen. Sin embargo, no se ha descubierto tal movimiento, así que es probable que las diferencias en las capturas de peces pequeños en las zonas costeras y mar afuera se deban principalmente a diferencias en la vulnerabilidad. Se descubrió poca o ninguna tendencia en los peces a desplazarse este-oeste o norte-sur. El método de Jones indica que el movimiento no es aleatorio, pero no revela una tendencia pronunciada a orientarse direccionalmente.

Growth of skipjack, Katsuwonus pelamis, and yellowfin, Thunnus albacares, tunas in the Eastern Pacific Ocean, as estimated from tagging data

ENGLISH: The average linear growth rate of skipjack in the eastern Pacific is less than 1 mm per day except for fish 375 to 424 mm in length at release. The growth rate shows a decrease with increasing length and increasing time at liberty. The growth rate of fish in the length range of about 43 to 57 cm is apparently more rapid in the eastern Pacific than in the western Pacific. Dsing data for the northeastern and southeastern Pacific combined, K and ~ were estimated to be 0.658 (on an annual basis) and 885 mm, respectively, by the ungrouped method and 0.829 and 846 mm, respectively, by the grouped method. Sensitivity analyses have shown however, that the estimates of these parameters are poorly determined by the sum of squares method used to derive them. Estimates of K and ~ for the eastern Pacific tend to be lower and higher, respectively, than those for the western Pacific. The average linear growth rate of yellowfin in the eastern Pacific is a little less than 1 mm per day for fish between about 25 and 100 cm in length at release. The growth appears to be most rapid in Area 2 (Revillagigedo Islands) and slowest in Areas 1 (Baja California), 5 (Central America- Colombia), and 6 (Ecuador-Peru). There is considerable variation in the growth rates of individual fish. The growth does not show a decrease with increasing length or increasing time at liberty so realistic estimates of the parameters of the von Bertalanffy or other similar equations cannot be calculated from these data. If realistic estimates of these parameters are to be secured larger fish must be tagged and released or many more long-term returns from fish to about 100 cm in length at release must be obtained. The growth patterns for the eastern Pacific, central Pacific and eastern Atlantic found by most other investigators differ from one another and from those found in the present study. Some of these differences may be real and others may be due to deficiencies in the data or the methods of analysis. Estimates obtained from tagging data are believed to be realistic provided the tags do not inhibit the growth of the fish. It appears that the growth rates of single- and double-tagged fish are the same; this indicates, though not unequivocally, that the tags do not inhibit the growth. SPANISH: La tasa media de crecimiento lineal del barrilete en el Pacífico oriental es inferior a lmm/día, excepto en el caso de peces de entre 375y 424mm de longitud de liberación. La tasa de crecimiento disminuye a medida que aumenta la longitud y el tiempo en libertad. La tasa de crecimiento de peces de entre unos 43 y 57 cm de longitud parece ser mayor en el Pacífico oriental que en el occidental. A partir de datos del Pacífico nororiental y suroriental combinados, se estimaron K y loo en 0.658 (anual) y 885mm, respectivamente, usando el método no agrupado, y 0.829 y 846mm, respectivamente, usando el método agrupado. Sin embargo, los análisis de sensitividad han demostrado que el método de suma de cuadrados utilizado para derivar las estimaciones de estos parámetros las determina con poca precisión. Las estimaciones de K y loo para el Pacífico oriental suelen ser inferiores y superiores, respectivamente, a los del Pacífico occidental. La tasa media de crecimiento lineal del aleta amarilla en el Pacífico oriental es ligeramente inferior a lmm/día para los peces de entre unos 25y 100cmde longitud de liberación. El crecimiento parece ser más rápido en el Area 2(Islas Revillagigedo),y más lento en las Areas 1(Baja California), 5 (Centroamérica-Colombia), y 6 (Ecuador-Perú). Las tasas de crecimiento de peces individuales varían considerablemente. El crecimiento no muestra una disminuciónconun aumento en la longitud o en el tiempo en libertad, y por consecuencia no se se pueden calcular estimaciones realistas de los parámetros de la ecuación de von Bertalanffy u otras ecuaciones similares a partir de estos datos. Para obtener estimaciones realistas de estos parámetros sería necesario marcar peces mayores u obtener muchas más devoluciones a largo plazo de marcas de peces de unos 100cm de longitud de liberación. Los patrones de crecimiento correspondientes al Pacífico oriental, Pacífico central, y Atlántico oriental descubiertos por la mayoría de los investigadores son diferentes entre síy también de los del presente estudio. Es posibleque algunas de estas diferencias sean verdaderas, mientras que otras se deban a faltas en los datos on en los métodos analíticos utilizados. Se considera que las estimaciones obtenidas a partir de los datos de marcado son realistas, suponiendo siempre que las marcas no impidan el crecimiento de los peces. Parece ser que las tasas de crecimiento de peces con una marca y con dos son idénticas, lo cual indica, aunque sin certeza total, que las marcas no ejercen tal efecto. (PDF contains 76 pages.)

Growth, movement, and attrition of northern bluefin tuna, Thunnus thynnus, in the Pacific Ocean, as determined by tagging

ENGLISH: The growth of northern bluefin tuna is described by a two-stanza model. For fish between 191 and 564 mm in length the Gompertz curve, with values of 581 mm and 4.32 for Loo and K (annual), respectively, is used. The fish between 564 and 1530 mm grow linearly, at the rate of 0.709 mm per day. Age-O fish tagged and released in the western Pacific Ocean have been recaptured in the western, central, and eastern Pacific. The minimum time between release in the western Pacific and recapture in the eastern Pacific is 215 days. Older fish, mostly Land 2-year olds, tagged and released in the eastern Pacific have been recaptured in the eastern and western Pacific. The minimum time between release in eastern Pacific and recapture in the western Pacific is 674 days. The coefficient of natural mortality is estimated from data on growth and ambient temperature to be 0.276 on an annual basis, with 90-percent confidence limits of 0.161 and 0.47L Spawning of northern bluefin takes place only in the western Pacific. Some of the juveniles migrate to the eastern Pacific, where they reside for several months to several years before returning to the western Pacific. The portion of fish which migrate to the eastern Pacific varies among years, and this appears to be an important cause of the annual variation in the catches in the eastern Pacific Ocean. SPANISH: El crecimiento del atún aleta azul del norte es descrito por un modelo de dos estadios. Para los peces de entre 191 y 564 mm de talla se usa la curva de Gompertz, con valores de 581 mm y 4.32 para Loo y K (anual), respectivamente. Los peces de entre 564 y 1530 mm crecen de forma lineal, a 0.709 mm por día. Peces de edad Omarcados y liberados en el Pacífico occidental han sido recapturados en el Pacífico occidental, central, y oriental. La demora mínima entre la liberación en el Pacífico occidental y la recaptura en el Pacífico oriental es de 215 días. Peces mayores, principalmente de 1 ó 2 años de edad, marcados y liberados en el Pacífico oriental han sido re capturados en el Pacífico occidental y oriental. La demora mínima entre la liberación en el Pacífico oriental y la recaptura en el Pacífico occidental es de 674 días. Se estima el coeficiente de mortalidad natural a partir de los datos de crecimiento y temperatura ambiental en un 0.276 anual, con límites de confianza al 90% de 0.161 y 0.471. El aleta azul del norte desova únicamente en el Pacífico occidental. Algunos de los juveniles migran al Pacífico oriental, donde permanecen entre varios meses y varios años antes de regresar al Pacífico occidental. La porción de los peces que migran al Pacífico oriental varía entre años, y ésto parece ser una causa importante de la variación anual en las capturas en el Océano Pacífico oriental. (PDF contains 94 pages.)

Quantitative, Time-Resolved Proteomic Analysis Using Bio-Orthogonal Non-Canonical Amino Acid Tagging

Bio-orthogonal non-canonical amino acid tagging (BONCAT) is an analytical method that allows the selective analysis of the subset of newly synthesized cellular proteins produced in response to a biological stimulus. In BONCAT, cells are treated with the non-canonical amino acid L-azidohomoalanine (Aha), which is utilized in protein synthesis in place of methionine by wild-type translational machinery. Nascent, Aha-labeled proteins are selectively ligated to affinity tags for enrichment and subsequently identified via mass spectrometry. The work presented in this thesis exhibits advancements in and applications of the BONCAT technology that establishes it as an effective tool for analyzing proteome dynamics with time-resolved precision.

Chapter 1 introduces the BONCAT method and serves as an outline for the thesis as a whole. I discuss motivations behind the methodological advancements in Chapter 2 and the biological applications in Chapters 2 and 3.

Chapter 2 presents methodological developments that make BONCAT a proteomic tool capable of, in addition to identifying newly synthesized proteins, accurately quantifying rates of protein synthesis. I demonstrate that this quantitative BONCAT approach can measure proteome-wide patterns of protein synthesis at time scales inaccessible to alternative techniques.

In Chapter 3, I use BONCAT to study the biological function of the small RNA regulator CyaR in Escherichia coli. I correctly identify previously known CyaR targets, and validate several new CyaR targets, expanding the functional roles of the sRNA regulator.

In Chapter 4, I use BONCAT to measure the proteomic profile of the quorum sensing bacterium Vibrio harveyi during the time-dependent transition from individual- to group-behaviors. My analysis reveals new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.

Overall, this work describes how to use BONCAT to perform quantitative, time-resolved proteomic analysis and demonstrates that these measurements can be used to study a broad range of biological processes.

An indexed bibliography of papers on tagging of tunas and billfishes

Two working parties, the Working Party on Tuna Tagging in the Pacific and Indian Oceans and the Working Party on Tuna Tagging in the Atlantic and Adjacent Seas, were formed by the Food and Agriculture Organization (FAD) of the United Nations in 1966 (Anonymous, 1966c). The conveners of these working parties were Dr. James Joseph of the Inter-American Tropical Tuna Commission (IATTC) and Mr. FrankJ. Mather, III, of the Woods Hole Oceanographic Institution (WHOI). In 1969 it was recommended that the working parties direct their attention toward billfishes, as well as tunas (Anonymous, 1969h: 5). One report (Joseph and working party, 1969) was published by the Pacific and Indian Oceans group and two (Mather and working party, 1969 and 1972) were published by the Atlantic Ocean and adjacent seas group. Each of the three working party reports included a bibliography of tuna and billfish tagging. The compiler of this bibliography, beginning in 1970, prepared numerous memoranda to the members of the working party, most of which included lists of papers on tuna and billfish tagging which had come to his attention, either directly or through members of the working party. The bibliographies in the three working party reports and the lists of references in the memoranda form the basis for the present bibliography.

Exploratory fishing for tunas and tuna tagging in the Marquesas, Tuamotu, Society, Pitcairn, and Gambier Islands

The Marquesas Islands are located in the Pacific Ocean at about 9 degrees south latitude and 140 degrees west longitude (Figure 1). It has been demonstrated by tagging (Anonymous, 1980b) that skipjack tuna, Katsuwonus pelamis, which occur in the northeastern Pacific Ocean have migrated to the Hawaiian Islands and Christmas Island in the central Pacific and also to the area between the Marshall and Mariana islands in the western Pacific. The Tuamotu, Society, Pitcairn, and Gambier islands, though the first two are not as close to the principal fishing areas of the eastern Pacific Ocean as are the Marquesas Islands, and the last two are small and isolated, are of interest for the same reasons that the Marquesas Islands are of interest, and thus skipjack should be tagged in those islands for the same reason that they should be tagged in the Marquesas Islands. The organizations which participated in the Marquesas Islands tagging and other scientific activities were the Inter-American Tropical Tuna Commission (IATTC), the South Pacific Commission (SPC), the Centre National pour l'Exploitation des Oceans (CNEXO), the Office de la Recherche Scientifique et Technique Outre-Mer (ORSTOM), the Service de la Peche de la Polynesie Francaise (SPPF), and the Service de l'Economie Rural (SER).

Growth of Skipjack tuna (Katsuwonus pelamis) in the eastern Pacific Ocean as estimated from tagging data

English: Data obtained from tagging experiments initiated during 1953-1958 and 1969-1981 for skipjack tuna from the coastal eastern Pacific Ocean (EPO) are reanalyzed, using the Schnute generalized growth model. The objective is to provide information that can be used to generate a growth transition matrix for use in a length-structured population dynamics model. The analysis includes statistical approaches to include individual variability in growth as a function of length at release and time at liberty, measurement error, and transcription error. The tagging data are divided into northern and southern regions, and the results suggest that growth rates differ between the two regions. The Schnute model provides a significantly better fit to the data than the von Bertalanffy model, a sub-model of the Schnute model, for the northern region, but not for the southern region. Individual variation in growth is best described as a function of time at liberty and as a function of growth increment for the northern and southern regions, respectively. Measurement error is a significant part of the total variation, but the results suggest that there is no bias caused by the measurement error. Additional information, particularly for small and large fish, is needed to produce an adequate growth transition matrix that can be used in a length-structured population dynamics model for skipjack tuna in the EPO. Spanish: Los datos obtenidos de los experimentos de marcado iniciados durante los períodos de 1953- 1958 y de 1969-1981 para el atún barrilete en las costas del Océano Pacífico Oriental (OPO) fueron analizados nuevamente, utilizando el modelo de crecimiento generalizado de Schnute. El objetivo es brindar información que sea útil para producir una matriz sobre la tran-sición de crecimiento que pueda utilizarse en un modelo de dinámica poblacional estructurado por talla. El análisis usa enfoques estadísticos para poder incluir la variabilidad individual del crecimiento como función de la talla de liberación y tiempo en libertad, el error de medición, y el error de transcripción. Los datos de marcado son divididos en regiones norte y sur, y los resultados sugieren que las tasas de crecimiento en las dos regiones son diferentes. En la región norte, pero no en la región sur, el modelo de Schnute se ajusta significativamente mejor a los datos que el modelo von Bertalanffy, un sub-modelo del modelo de Schnute. La mejor descripción de la variación individual en el crecimiento es como una función del tiempo en libertad y como una función del incremento de crecimiento para las regiones norte y sur, respectivamente. El error de medición es una parte significativa de la variación total, pero los resultados sugieren que no existe un sesgo causado por el error de medición. Se necesita información adicional, particularmente para peces pequeños y grandes, para poder producir una matriz de transición de crecimiento adecuada que pueda utilizarse en el modelo de dinámica poblacional estructurado por tallas para el atún barrilete en el OPO.

A preliminary investigation of Elastomer Visible Implant (EVI)tag retention rates and the effect of tagging on the growth and survival of barbel (Barbus barbus L.).

The Elastomer Visible Implant system (EVI) is a relatively new technique for batch marking fish. The aim of this study was to assess retention rates and the possible effects of tagging on the growth and mortality of barbel, Barbus barbus, (81-197mm, fork length) over approximately 2 months using a syringe injection system.

Comparison of six tag types in sea-trout tagging experiments in the Baltic Sea

In the international Baltic Sea trout tagging experiment 27 753 hatchery reared sea trout smolts were tagged in Denmark, Finland, Poland and Sweden in 1979 and 1980. The fish were tagged with the original Carlin tag, two modified Carlin tag types (Canadian and Finnish polythene), streamer and Floy tags and Polish tags attached with Monel metal wire. The tag returns were affected by the place of release and smolt quality. The best results were obtained in the case of tags attached with double wire or thread -original Carlin, Canadian and Finnish polythene. The poorest results were obtained with streamer tags.

Variation in movement patterns of red drum (Sciaenops ocellatus) inferred from conventional tagging and ultrasonic telemetry

We used 25 years of conventional tagging data (n= 6173 recoveries) and 3 years of ultrasonic telemetry data (n=105 transmitters deployed) to examine movement rates and directional preferences of four age classes of red drum (Sciaenops ocellatus) in estuarine and coastal waters of North Carolina. Movement rates of conventionally tagged red drum were dependent on the age, region, and season of tagging. Age-1 and age-2 red drum tagged along the coast generally moved along the coast, whereas fish tagged in oligohaline waters far from the coast were primarily recovered in coastal regions in fall months. Adult (age-4+) red drum moved from overwintering grounds on the continental shelf through inlets into Pamlico Sound in spring and summer months and departed in fall. Few tagged red drum were recovered in adjacent states (0.6% of all recoveries); however, some adult red drum migrated seasonally from overwintering grounds in coastal North Carolina northward to Virginia in spring, returning in fall. Age-2 transmitter-tracked red drum displayed seasonal emigration from a small tributary, but upstream and downstream movements within the tributary were correlated with fluctuating salinity regimes and not season. Large-scale conventional tagging and ultrasonic telemetry programs can provide valuable insights into the complex movement patterns of estuarine fish.

Defining plausible migration rates based on historical tagging data: a Bayesian mark-recapture model applied to English sole (Parophrys vetulus)

A generalized Bayesian population dynamics model was developed for analysis of historical mark-recapture studies. The Bayesian approach builds upon existing maximum likelihood methods and is useful when substantial uncertainties exist in the data or little information is available about auxiliary parameters such as tag loss and reporting rates. Movement rates are obtained through Markov-chain Monte-Carlo (MCMC) simulation, which are suitable for use as input in subsequent stock assessment analysis. The mark-recapture model was applied to English sole (Parophrys vetulus) off the west coast of the United States and Canada and migration rates were estimated to be 2% per month to the north and 4% per month to the south. These posterior parameter distributions and the Bayesian framework for comparing hypotheses can guide fishery scientists in structuring the spatial and temporal complexity of future analyses of this kind. This approach could be easily generalized for application to other species and more data-rich fishery analyses.