Evaluation of dengue NS1 antigen detection for diagnosis in public health laboratories, São Paulo State, 2009

The present work evaluated the diagnostic accuracy of detection of Dengue NS1 antigen employing two NS1 assays, an immunochromatographic assay and ELISA, in the diagnostic routine of Public Health laboratories. The results obtained with NS1 assay were compared with virus isolation and, in a subpopulation of cases, they were compared with the IgM-ELISA results obtained with convalescent samples. A total of 2,321 sera samples were analyzed by one of two NS1 techniques from March to October 2009. The samples were divided into five groups: groups I, II and III included samples tested by NS1 and virus isolation, and groups IV and V included patients with a first sample tested by NS1 and a second sample tested by IgM-ELISA. Sensitivity, specificity, positive and negative predictive values, Kappa Index and Kappa Concordance were calculated. The results showed that NS1 testing in groups I, II and III had high sensitivity (98.0%, 99.5% and 99.3%), and predictive values and Kappa index between 0.9 - 1.0. Groups IV and V only had Kappa Concordance calculated, since the samples were analyzed according to the presence of NS1 antigen or IgM antibody. Concordance of 92.1% was observed when comparing the results of NS1-negative samples with IgM-ELISA. Based on the findings, it is possible to suggest that the tests for NS1 detection may be important tools for monitoring the introduction and spread of Dengue serotypes.

Los entornos virtuales como herramienta sustituta del laboratorio "real" y como complemento de la enseñanza de Química a alumnos de Ingeniería. Virtual environments as substitute tools for the "conventional" laboratories and as a complement for Chemistry teaching to Engineering students.

El presente proyecto se plantea el siguiente problema de investigación:¿Cuál es la eficacia de los entornos virtuales de enseñanza para optimizar los aprendizajes de Química? Se sostiene la hipótesis de que los entornos virtuales de enseñanza, empleados como mediación instrumental, son eficaces para optimizar los aprendizajes de química, particularmente facilitando la vinculación y reversibilidad entre "mundo micro y macroscópico"; capacidad que usualmente sólo se atribuye al trabajo experimental de laboratorio. Los objetivos propuestos son: Determinar la eficacia de entornos virtuales de enseñanza, como mediaciones instrumentales, para optimizar los aprendizajes de química en estudiantes de ingeniería. Implementar un entrono virtual de enseñanza de química, diseñado como mediación instrumental y destinado a estudiantes de dos carreras de ingeniería del IUA. Evaluar el desarrollo y los resultados de la innovación introducida. Comparar los resultados de la innovación con los resultados de la enseñanza usual. Derivar conclusiones acerca de la eficacia de la innovación propuesta. Socializar el conocimiento producido en ámbitos científico-tecnológicos reconocidos. Se generará un aula virtual en plataforma Educativa y utilidzando el laboratorio de computación de la institución se buscará desarrollar laboratorios virtuales donde se propondrán actividades de simulación de trabajo experimental. Los resultados esperados son: - Un Aula Virtual que cumpla funciones análogas a las de un laboratorio experimental. - Información válida y confiable acerca de la eficacia de la misma como medio para optimizar los aprendizajes de química. - Publicaciones en ámbitos científico-tecnológicos reconocidos que sometan a juicio público la innovación y la investigación efectuadas. La importancia del proyecto radica principalmente en poner a prueba la eficacia de los entornos virtuales para optimizar los aprendizajes de química, analogando tareas usualmente limitadas al trabajo experimental de laboratorio. Su pertinencia apunta a un replanteo del curriculo de los cursos de Química para estudiantes de Ingeniería.

Music and health in Kenya : sound, spirituality and altered consciousness juxtaposed with emotions

Music and Healing, African Music, Music therapy, Healing Rituals, Kenyan Music

Conference on the Matter of Pollution of the Navigable Waters of Puget Sound, the Strait of Juan de Fuca and Their Tributaries and Estuaries. 2d, Seattle, Sept. 6-7, and Oct. 6, 1967: Proceedings.

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Conference on the Matter of Pollution of the Navigable Waters of Puget Sound, the Strait of Juan de Fuca and Their Tributaries and Estuaries. 2d, Seattle, Sept. 6-7, and Oct. 6, 1967: Proceedings.

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Conference on the Matter of Pollution of the Navigable Waters of Puget Sound, the Strait of Juan de Fuca and Their Tributaries and Estuaries. 2d, Seattle, Sept. 6-7, and Oct. 6, 1967: Proceedings.

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A comparison of simian rotavirus SA11 preparations maintained in different laboratories

Preparations of simian SA11 maintained in different laboratories were compared with each other by polyacrylamide gel electrophoresis of genomic RNA. Differences in the migration of genome segments 4,5 and 7 allowed the classification of eight virus preparations into four electrophoretic types.

The role of the right parietal cortex in sound localization: a chronometric single pulse transcranial magnetic stimulation study.

Auditory spatial functions, including the ability to discriminate between the positions of nearby sound sources, are subserved by a large temporo-parieto-frontal network. With the aim of determining whether and when the parietal contribution is critical for auditory spatial discrimination, we applied single pulse transcranial magnetic stimulation on the right parietal cortex 20, 80, 90 and 150 ms post-stimulus onset while participants completed a two-alternative forced choice auditory spatial discrimination task in the left or right hemispace. Our results reveal that transient TMS disruption of right parietal activity impairs spatial discrimination when applied at 20 ms post-stimulus onset for sounds presented in the left (controlateral) hemispace and at 80 ms for sounds presented in the right hemispace. We interpret our finding in terms of a critical role for controlateral temporo-parietal cortices over initial stages of the building-up of auditory spatial representation and for a right hemispheric specialization in integrating the whole auditory space over subsequent, higher-order processing stages.

Towards an emotion-driven interactive music system: ridging the gaps between affect, physiology and sound generation

Report for the scientific sojourn at the Stanford University from January until June 2007. Music is well known for affecting human emotional states, yet the relationship between specific musical parameters and emotional responses is still not clear. With the advent of new human-computer interaction (HCI) technologies, it is now possible to derive emotion-related information from physiological data and use it as an input to interactive music systems. Providing such implicit musical HCI will be highly relevant for a number of applications including music therapy, diagnosis, nteractive gaming, and physiologically-based musical instruments. A key question in such physiology-based compositions is how sound synthesis parameters can be mapped to emotional states of valence and arousal. We used both verbal and heart rate responses to evaluate the affective power of five musical parameters. Our results show that a significant correlation exists between heart rate and the subjective evaluation of well-defined musical parameters. Brightness and loudness showed to be arousing parameters on subjective scale while harmonicity and even partial attenuation factor resulted in heart rate changes typically associated to valence. This demonstrates that a rational approach to designing emotion-driven music systems for our public installations and music therapy applications is possible.

The spatio-temporal brain dynamics of processing and integrating sound localization cues in humans.

Interaural intensity and time differences (IID and ITD) are two binaural auditory cues for localizing sounds in space. This study investigated the spatio-temporal brain mechanisms for processing and integrating IID and ITD cues in humans. Auditory-evoked potentials were recorded, while subjects passively listened to noise bursts lateralized with IID, ITD or both cues simultaneously, as well as a more frequent centrally presented noise. In a separate psychophysical experiment, subjects actively discriminated lateralized from centrally presented stimuli. IID and ITD cues elicited different electric field topographies starting at approximately 75 ms post-stimulus onset, indicative of the engagement of distinct cortical networks. By contrast, no performance differences were observed between IID and ITD cues during the psychophysical experiment. Subjects did, however, respond significantly faster and more accurately when both cues were presented simultaneously. This performance facilitation exceeded predictions from probability summation, suggestive of interactions in neural processing of IID and ITD cues. Supra-additive neural response interactions as well as topographic modulations were indeed observed approximately 200 ms post-stimulus for the comparison of responses to the simultaneous presentation of both cues with the mean of those to separate IID and ITD cues. Source estimations revealed differential processing of IID and ITD cues initially within superior temporal cortices and also at later stages within temporo-parietal and inferior frontal cortices. Differences were principally in terms of hemispheric lateralization. The collective psychophysical and electrophysiological results support the hypothesis that IID and ITD cues are processed by distinct, but interacting, cortical networks that can in turn facilitate auditory localization.