Deep tissue fluorescence imaging with time-reversed light

Advances in optical techniques have enabled many breakthroughs in biology and medicine. However, light scattering by biological tissues remains a great obstacle, restricting the use of optical methods to thin ex vivo sections or superficial layers in vivo. In this thesis, we present two related methods that overcome the optical depth limit—digital time reversal of ultrasound encoded light (digital TRUE) and time reversal of variance-encoded light (TROVE). These two techniques share the same principle of using acousto-optic beacons for time reversal optical focusing within highly scattering media, like biological tissues. Ultrasound, unlike light, is not significantly scattered in soft biological tissues, allowing for ultrasound focusing. In addition, a fraction of the scattered optical wavefront that passes through the ultrasound focus gets frequency-shifted via the acousto-optic effect, essentially creating a virtual source of frequency-shifted light within the tissue. The scattered ultrasound-tagged wavefront can be selectively measured outside the tissue and time-reversed to converge at the location of the ultrasound focus, enabling optical focusing within deep tissues. In digital TRUE, we time reverse ultrasound-tagged light with an optoelectronic time reversal device (the digital optical phase conjugate mirror, DOPC). The use of the DOPC enables high optical gain, allowing for high intensity optical focusing and focal fluorescence imaging in thick tissues at a lateral resolution of 36 µm by 52 µm. The resolution of the TRUE approach is fundamentally limited to that of the wavelength of ultrasound. The ultrasound focus (~ tens of microns wide) usually contains hundreds to thousands of optical modes, such that the scattered wavefront measured is a linear combination of the contributions of all these optical modes. In TROVE, we make use of our ability to digitally record, analyze and manipulate the scattered wavefront to demix the contributions of these spatial modes using variance encoding. In essence, we encode each spatial mode inside the scattering sample with a unique variance, allowing us to computationally derive the time reversal wavefront that corresponds to a single optical mode. In doing so, we uncouple the system resolution from the size of the ultrasound focus, demonstrating optical focusing and imaging between highly diffusing samples at an unprecedented, speckle-scale lateral resolution of ~ 5 µm. Our methods open up the possibility of fully exploiting the prowess and versatility of biomedical optics in deep tissues.

一种新型结构的单纵模光纤激光器

单纵模掺铒光纤激光器在光通信和光传感等方面有着广泛的应用前景。设计了一种新型的光纤激光器，在光纤环形镜中嵌入未抽运的掺铒光纤作为可饱和吸收体以抑制多纵模，用光纤环谐振腔作为滤波器抑制拍频噪声，用光纤光栅作为波长选择器件，最终得到了单纵模输出并消除了拍频噪声。使用零拍法测量其线宽小于频谱仪的低频极限5kHz。实验结果证明了可饱和吸收体和光纤环的功能。

Non-Linear Scale Interactions in a Forced Turbulent Boundary Layer

This thesis explores the dynamics of scale interactions in a turbulent boundary layer through a forcing-response type experimental study. An emphasis is placed on the analysis of triadic wavenumber interactions since the governing Navier-Stokes equations for the flow necessitate a direct coupling between triadically consist scales. Two sets of experiments were performed in which deterministic disturbances were introduced into the flow using a spatially-impulsive dynamic wall perturbation. Hotwire anemometry was employed to measure the downstream turbulent velocity and study the flow response to the external forcing. In the first set of experiments, which were based on a recent investigation of dynamic forcing effects in a turbulent boundary layer, a 2D (spanwise constant) spatio-temporal normal mode was excited in the flow; the streamwise length and time scales of the synthetic mode roughly correspond to the very-large-scale-motions (VLSM) found naturally in canonical flows. Correlation studies between the large- and small-scale velocity signals reveal an alteration of the natural phase relations between scales by the synthetic mode. In particular, a strong phase-locking or organizing effect is seen on directly coupled small-scales through triadic interactions. Having characterized the bulk influence of a single energetic mode on the flow dynamics, a second set of experiments aimed at isolating specific triadic interactions was performed. Two distinct 2D large-scale normal modes were excited in the flow, and the response at the corresponding sum and difference wavenumbers was isolated from the turbulent signals. Results from this experiment serve as an unique demonstration of direct non-linear interactions in a fully turbulent wall-bounded flow, and allow for examination of phase relationships involving specific interacting scales. A direct connection is also made to the Navier-Stokes resolvent operator framework developed in recent literature. Results and analysis from the present work offer insights into the dynamical structure of wall turbulence, and have interesting implications for design of practical turbulence manipulation or control strategies.

单纵模相移DFB掺Yb3+光纤激光器运行特性的研究

对我们所制作的λ/4相移DFB掺Yb3+光纤激光器的运行特性进行了研究。研究表明：光纤端面菲涅尔反射会破坏激光器的单纵模运行,因此为获得稳定的羊纵模运行须使用隔离器或甘油清除光纤端面菲涅尔反射；机械扰动则会使沿光纤传输的单偏振激光的偏振面发生变化；温度的涨落则会引起激光输出功率的不稳定涨落。所研制à/4相移DFB单纵模、单偏振激光器具有如下特性：阈值为38mW,当泵浦功率为140 mW时,获得了25mW的1053 nm单 纵模、羊偏振激光.偏振消光比约30 dB,单纵模激光功率涨落小于2%,边模抑制比约6

基于光纤环形镜的全光脉冲整形器

提出了一种基于光纤环形镜的全光脉冲整形器。该全光脉冲整形器利用波分复用器将控制光脉冲引入光纤环形镜中，控制光脉冲由于交叉相位调制在信号光上产生了非线性附加相移。信号光在耦合器中发生干涉，经过整形的信号光脉冲从脉冲整形器的出射端出射，信号脉冲的波形由非线性附加相移的波形决定。实验中．利用对控制脉冲光谱整形和啁啾展宽的方法来对控制脉冲进行时间脉冲的整形，该全光脉冲整形器实现了对单纵模激光的脉冲整形，同时实现了飞秒脉冲和单纵模整形脉冲的精确同步。在理论上数值计算了该全光脉冲整形器的输出特性，理论计算结果和实验结

基于高频微波技术的分布式光纤传感器布里渊散射信号检测

分析了布里渊分布式光纤传感技术原理,采用自行研制的光纤单纵模分布反馈(DFB)激光器结合电光调制技术,利用相干检测技术,对布里渊微弱后向散射信号进行检测。通过改进滤波放大技术,对微弱后向散射光信号进行有效放大,再用扰偏技术及信号采样平均处理,实现对光纤传感器后向布里渊散射信号在11 GHz高频段直接采集显示。结果表明,探测所得布里渊散射信号峰值功率可达50 mV,能有效降低解调系统信号检测难度,改善了系统信噪比(SNR)。初步实验结果证明了该方案的可行性。

大模场面积多模光纤激光器的横模控制

缠绕技术通常被用于大模场面积多模光纤激光器的横模控制。将光纤弯曲成不同半径的圆环，多模光纤激光器的高阶横模逐个被抑制并在缠绕半径为20mm时，获得15.4W的单模激光输出。实验研究表明，缠绕半径减小时，由于激光器高阶模式被抑制，其光束质量变好，同时其斜率效率降低。实验测量了多模光纤激光器在不同缠绕半径下的输出性能，并理论计算了各阶模式在不同缠绕半径下的损耗特性。实验测量结果与理论计算结果符合得较好。

大模场面积光纤激光器79．4W单横模输出

abstract {A large-mode-area (LMA) multimode fiber before and after coiling was studied contrastively in the experiment. Single-transverse-mode output was achieved when the fiber laser was coiled around a mandrel of 65 mm radius. After coiling, beam quality factor of the laser dropped from 1.24 to 1.06 and slope efficiency dropped from 64.7% to 54.3%. When the launched pump power was 149 W, the corresponding output power was 94.7 W and 79.4 W, respectively. However, the brightness of the coiled fiber laser was 1.15 times that of the uncoiled. Coiled modal losses of different modes were also calculated for the fiber employed in the experiment. The measured results agree well with the calculated ones.}

侧面泵浦的短矩形掺钕磷酸盐玻璃光纤激光器

利用光纤－波导耦合器技术侧面泵浦一根4厘米长的短光纤，我们研制得到了一个红外波长的光纤激光器。该激光器的增益介质是横截面为矩形的掺钕磷酸盐玻璃光纤，其纤芯横截面尺寸为1.5×0.5 毫米，数值孔径为0.2。单横模激光可以通过该矩形光纤的增益-导引效应来获得。最大的激光输出功率为1.05瓦，斜率效率为10％。

All solid-state, injection-seeded Ti:sapphire ring laser

In this letter, we present an all solid-state, injection-seeded Ti:sapphire laser. The laser is pumped by a laser diode pumped frequency-doubled Nd:YAG laser, and injection-seeded by an external cavity laser diode with the wavelength between 770 and 780 nm. The single longitude mode and the doubling efficiency of the laser are obtained after injection seeding. The experimental setup and relative results are reported. It is a good candidate laser source for mobile differential absorption lidar (DIAL) system.

A compact diode-pumped injection seeded Nd : YAG laser with resonance-detection technique

A diode pumped injection seeded single-longitudinal-mode (SLM) Nd:YAG laser is achieved by using the resonance-detection technique in Q-switching operation. The pulsed oscillator laser uses a folded cavity to achieve compact construction. This system operates at 100 Hz and provides over 20 mJ/pulse of single-frequency 1064 nm output. The M-2 values of horizontal and vertical axes are 1.58 and 1.41, respectively. The probability of putting out single-longitudinal-mode pulses is 100%. The 355 nm laser output produced by frequency tripling has a linewidth less than 200 MHz. The laser can run over eight hours continually without mode hopping.

Demonstration of microfiber knot laser

The authors demonstrate a 1.5 mu m wavelength microfiber laser formed by tightening a doped microfiber into a knot in air. The 2-mm-diameter knot, assembled using a 3.8-mu m-diameter microfiber that is directly drawn from Er:Yb-doped phosphate glass, serves as both active medium and resonating cavity for lasing. Single-longitudinal-mode laser with threshold of about 5 mW and output power higher than 8 mu W is obtained. Their initial results suggest a simple approach to highly compact lasers based on doped microscale optical fibers. (c) 2006 American Institute of Physics.

Laser-induced damage of Ta2O5/S1O2 narrow-band interference filters under different 1064 nm Nd : YAG laser modes

The laser-induced damage (LID) behavior of narrow-band interference filters was investigated with a Nd:YAG laser at 1064 nm under single-pulse mode and free-running mode. The absorption measurement of such coatings was performed with surface thermal lensing (STL) technique. The damage morphologies under the two different laser modes were also studied in detail. It was found that all the filters exhibited a pass-band-center-dependent absorption and laser-induced damage threshold (LIDT) behavior, but the damage morphologies were diverse. The explanation was given with the analysis of the electric field distribution and the operational behavior of the irradiation laser. (c) 2005 Elsevier B.V. All rights reserved.

Ta2O5／SiO2硬膜双腔滤光片激光损伤特性研究

研究了Ta2O5／SiO2硬膜双腔干涉滤光片带内、带边及带外的吸收和激光损伤特性。实验发现，对于作用激光，带通滤光片的驻波场分布、吸收率和损伤阈值在带内、带边和带外的响应特性对作用激光波长均呈现出明显的选择性。根据实验结果，结合滤光片的驻波场分析，给出了带通滤光片的损伤机理。

2D-lattice distributed reflector laser

A single-contact, mode-hop-free, single longitudinal mode laser operating cw under large signal modulation at 2.5 Gbit/s at room temperature was created by introducing a short etched region around the ridge-waveguide of a Fabry-Perot laser. The device could be suitable for use in extended range data communications applications.