Statistical prediction of single-stranded regions in RNA secondary structure and application to predicting effective antisense target sites and beyond

Single-stranded regions in RNA secondary structure are important for RNA–RNA and RNA–protein interactions. We present a probability profile approach for the prediction of these regions based on a statistical algorithm for sampling RNA secondary structures. For the prediction of phylogenetically-determined single-stranded regions in secondary structures of representative RNA sequences, the probability profile offers substantial improvement over the minimum free energy structure. In designing antisense oligonucleotides, a practical problem is how to select a secondary structure for the target mRNA from the optimal structure(s) and many suboptimal structures with similar free energies. By summarizing the information from a statistical sample of probable secondary structures in a single plot, the probability profile not only presents a solution to this dilemma, but also reveals ‘well-determined’ single-stranded regions through the assignment of probabilities as measures of confidence in predictions. In antisense application to the rabbit β-globin mRNA, a significant correlation between hybridization potential predicted by the probability profile and the degree of inhibition of in vitro translation suggests that the probability profile approach is valuable for the identification of effective antisense target sites. Coupling computational design with DNA–RNA array technique provides a rational, efficient framework for antisense oligonucleotide screening. This framework has the potential for high-throughput applications to functional genomics and drug target validation.

Association of secondary glioblastoma and pituitary adenoma : case report

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Characterization of priority pollutants from a secondary lead and battery manufacturing facility /

Jan. 1979.

Studies in the Gospel according to Mark : for the use of classes in secondary schools and in the secondary division of the Sunday school /

Mode of access: Internet.

Secondary math and science education [microform] : hearing before the Subcommittee on Science, Research, and Technology of the Committee on Science and Technology, U.S. House of Representatives, Ninety-seventh Congress, second session, May 7, 1982.

"No. 169."

Life adjustment education for every youth : prepared in the Division of secondary education...and Division of vocational education.

Mode of access: Internet.

Public elementary and secondary schools and agencies in the United States and outlying areas: school year... /

Description based on: 1990-91.

Report of Committee on college entrance requirements July, 1899 : appointed by Departments of secondary education and higher education at Denver meeting July, 1895.

At head of title: National educational association.

Algebra; an elementary text-book, for the higher classes of secondary schools and for colleges.

Mode of access: Internet.

Report on secondary schools and pupil-teacher centres (existing conditions). With introductory notes.

Mode of access: Internet.

Systematizing the work of school principals for elementary and secondary school and junior college executives and their assistants,

Mode of access: Internet.

Karyotype and NOR-banding of mitotic chromosomes of some Vitis L. species

Chromosome studies were performed in V. champinii, V. cinerea, V. girdiana, V. labrusca, V. rotundifolia, V. rupestris and V. vinifera with the purpose of species characterization using chromosome morphometric data and NOR banding. A median ideogram was obtained for each species. The karyotype formula obtained varied from 7m + 12sm to 9m + 11sm. The species showed moderate chromosome asymmetry values according to TF% form, Stebbins, Romero Zarco and Paszko indices. V. champinii and V. girdiana were apart from the other species by CVcl and CVci graphic representation and also formed a group apart in the dendrogram based on Euclidian distances. The chromosome pair number 3 harbors the secondary constriction and a satellite segment in all species analyzed with Giemsa staining and it may be the same observed after NOR banding technique. It seems that the process of speciation in the North American Euvitis species studied involved some discrete changes in chromosome morphometry which have been reflected in the asymmetry index.

Native species indicated for degraded area recovery in Western Paraná, Brazil

Colonization in the State of Paraná has culminated in the devastation of large forest areas in the entire State. Degraded area recovery programs have emphasized the utilization of native species, but often the species indicated for local reforestation areas are unknown, as those areas are little known floristically. This study aimed to survey native species indicated for reforestation of areas in the Western region of the State of Paraná, classify those species as pioneer, secondary, or climactic, and indicate places of occurrence of matrices where seeds of those species could be collected. Bibliographic surveys in the specialized literature and research in the Herbarium Museu Botânico Municipal de Curitiba (MBM) and Herbarium of Universidade Estadual do Oeste do Paraná (UNOP) were conducted to identify potential species for degraded area recovery in the study of Western region of Paraná. In all, 115 species were selected, of which 22 are pioneer, 73 are secondary, and 20 are climactic. The bibliographic surveys suggests that pioneer species are the most indicated for the initial processes in the degraded areas recovery, while secondary and climactic species play a major role in area enrichment.

Two new species and one new synonym of Maytenus (Celastraceae) from Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)