Isolamento e caracterização de Salmonella sp. em suínos ao abate e em cortes de pernil

A Salmonella sp. é uma das principais causas de doenças transmitidas por alimentos, principalmente de origem animal. Os suínos podem ser portadores assintomáticos de Salmonella sp. e, nessa condição, serem levados ao abate. Desta forma, a entrada de Salmonella sp. na cadeia de produção é uma preocupação para a indústria. Sabendo-se da importância deste microrganismo, pelo seu impacto na indústria e na saúde pública, este trabalho teve como objetivo determinar a prevalêncía de Salmonella sp. em suínos levados ao abate em um frigorífico sob inspeção federal no Rio Grande do Sul, e correlacionar com a contaminação de cortes de pernil. Para tanto, 64 amostras de conteúdo intestinal e 121 amostras de cortes de pernil coletadas em 4 visitas, foram submetidas ao isolamento e identificação de Salmonella sp. As amostras de Salmonella sp. foram avaliadas quanto a resistência a 14 antimicrobianos, através da técnica de difusão em ágar. Salmonella sp. foi isolada de 46,87% das amostras de conteúdo intestinal e de 49,59% dos cortes de pernil. Foi encontrado grande número de sorovares, sendo os mais prevalentes Panama e Bredeney. Das amostras isoladas, 60,8% apresentaram perfil de multiresistência, sendo os maiores índices de resistência a sulfonamida, ácido nalidíxico e tetraciclina. Utilizando o perfil de resistência a antimicrobianos de amostras de um mesmo sorovar forma comparadas quanto a sua similaridade e agrupadas em dendrogramas, observou-se grande diversidade nas linhagens isoladas. Os resultados demonstraram que a entrada de animais no frigorífico excretando Salmonella sp., pode contribuir para a contaminação do produto final. A diversidade de sorovares e linhagens pode representar que são várias as fontes de contaminação, tanto dos animais quanto do produto final.

Silver Nanocomposite Electrode Modified with Hexacyanoferrate. Preparation, Characterization and Electrochemical Behaviour Towards Substituted Anilines

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Parameters affecting sulfonamide photo-Fenton degradation - Iron complexation and substituent group

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Delayed results of treatment of paracoccidioidomycosis with amphotericin B plus sulfamides versus amphotericin B alone

Os Autores avaliam tardiamente dois grupos de doentes de paracoccidioidomicose. Ambos foram tratados com anfotericina B, tendo um deles feito tratamento de manutenção com sulfamídicos. Através de análises estatísticas comprovam a maior eficácia do tratamento, quando se faz a manutenção com sulfamídicos.

New Ni(II)-sulfonamide complexes: Synthesis, structural characterization and antibacterial properties. X-ray diffraction of [Ni(sulfisoxazole)(2)(H2O)(4)] center dot 2H(2)O and [Ni(sulfapyridine)(2)]

The synthesis, structural characterization, voltammetric experiments and antibacterial activity of [Ni(sulfisoxazole)(2)(H2O)(4)] center dot 2H(2)O and [Ni(sulfapyridine)(2)] were studied and compared with similar previously reported copper complexes. [Ni(sulfisoxazole)(2)(H2O)(4)] center dot 2H(2)O crystallized in a monoclinic system, space group C2/c where the nickel ion was in a slightly distorted octahedral environment, coordinated with two sulfisoxazole molecules through the heterocyclic nitrogen and four water molecules. [Ni(sulfapyridine)(2)] crystallized in a orthorhombic crystal system, space group Pnab. The nickel ion was in a distorted octahedral environment, coordinated by two aryl amine N from two sulfonamides acting as monodentate ligands and four N atoms (two sulfonamidic N and two heterocyclic N) from two different sulfonamide molecules acting as bidentate ligands. Differential pulse voltammograms were recorded showing irreversible peaks at 1040 and 1070 mV, respectively, attributed to Ni(II)/Ni(III) process. [Ni(sulfisoxazole)(2)(H2O)(4)] center dot 2H(2)O and [Ni(sulfapyridine)(2)] presented different antibacterial behavior against Staphylococcus aureus and Escherichia coli from the similar copper complexes and they were inactive against Mycobacterium tuberculosis. (c) 2007 Elsevier B.V. All rights reserved.

β-conglycinin combined with fenofibrate or rosuvastatin have exerted distinct hypocholesterolemic effects in rats

Background: There is increasing interest in non-pharmacological control of cholesterol and triglyceride levels in the plasma and diet-drug association represent an important area of studies. The objective of this study was to observe the hypocholesterolemic effect of soybean β-conglycinin (7S protein) alone and combined with fenofibrate and rosuvastatin, two hypolipidemic drugs. Methods. The protein and drugs were administered orally once a day to rats and the effects were evaluated after 28 days. Wistar rats were divided into six groups (n = 9): hypercholesterolemic diet (HC), HC+7S protein (300 mg.kg-1 day-1) (HC-7S), HC+fenofibrate (30 mg.kg-1 day-1)(HC-FF), HC+rosuvastatin (10 mg.kg-1 day-1)(HC-RO), HC+7S+fenofibrate (HC-7S-FF) and HC+7S+rosuvastatin (HC-7S-RO). Results: Animals in HC-7S, HC-FF and HC-RO exhibited reductions of 22.9, 35.8 and 18.8% in total plasma cholesterol, respectively. In HC-7S-FF, animals did not show significant alteration of the level in HC+FF while the group HC-7S-RO showed a negative effect in comparison with groups taking only protein (HC-7S) or drug (HC-RO). The administration of the protein, fenofibrate and rosuvastatin alone caused increases in the plasma HDL-C of the animals, while the protein-drug combinations led to an increase compared to HC-FF and HC-RO. The plasma concentration of triacylgycerides was significantly reduced in the groups without association, while HC-7S-FF showed no alteration and HC-7S-RO a little reduction. Conclusion: The results of our study indicate that conglycinin has effects comparable to fenofibrate and rosuvastatin on the control of plasma cholesterol, HDL-C and triacylglycerides, when given to hypercholesterolemic rats, and suggests that the association of this protein with rosuvastatin alters the action of drug in the homeostasis of cholesterol. © 2012 Ferreira et al; licensee BioMed Central Ltd.

Study of a series of cobalt(II) sulfonamide complexes: Synthesis, spectroscopic characterization, and microbiological evaluation against M. tuberculosis. Crystal structure of [Co(sulfamethoxazole)2(H 2O)2]·H2O

Nowadays, the research for new and better antimicrobial compounds is an important field due to the increase of immunocompromised patients, the use of invasive medical procedures and extensive surgeries, among others, that can affect the incidence of infections. Another big problem associated is the occurrence of drug-resistant microbial strains that impels a ceaseless search for new antimicrobial agents. In this context, a series of heterocyclic- sulfonamide complexes with Co(II) was synthesized and characterized with the aim of obtaining new antimicrobial compounds. The structural characterization was performed using different spectroscopic methods (UV-Vis, IR, and EPR). In spite of the fact that the general stoichiometry for all the complexes was Co(sulfonamide)2·nH2O, the coordination atoms were different depending on the coordinated sulfonamide. The crystal structure of [Co(sulfamethoxazole)2(H2O)2]·H 2O was obtained by X-ray diffraction showing that Co(II) is in a slightly tetragonal distorted octahedron where sulfamethoxazole molecules act as a head-to-tail bridges between two cobalt atoms, forming polymeric chains. Besides, the activity against Mycobacterium tuberculosis, one of the responsible for tuberculosis, and the cytotoxicity on J774A.1 macrophage cells were evaluated. © 2012 Elsevier B.V. All rights reserved.

Estrutura eletrônica de materiais orgânicos: moléculas antimalariais de sulfonamidas e anilinoquinolinas

Pós-graduação em Ciência e Tecnologia de Materiais - FC

Desenvolvimento de sistemas de screening utilizando procedimentos em fluxo para monitoramento de sulfonamidas em urina e produtos de origem animal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento de sistemas de screening automatizados para determinação de sulfonamidas em mel e procedimento limpo para a quantificação de sulfaquinoxalina em medicamentos veterinários

Pós-graduação em Química - IQ

Desenvolvimento de métodos limpos para screening e determinação de sulfonamidas em matrizes diversas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon

ABSTRACT: The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described.

Desenvolvimento de métodos para determinação de sulfonamidas, dipirona e citrato de sildenafil em matrizes diversas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Degradação de antibióticos sulfonamidas por processo foto-Feton: identificação de intermediários

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)