Análise de séries temporais de coordenadas estimadas com GPS: uma proposta metodológica para eliminação de efeitos sazonais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de wavelets para detecção e correção do multicaminho no posicionamento relativo GNSS estático e cinemático

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Investigações sobre o impacto da modernização do GNSS no Posicionamento

Pós-graduação em Ciências Cartográficas - FCT

Solução rápida das ambigüidades GPS para aplicações no posicionamento relativo de linhas de base curtas

Pós-graduação em Ciências Cartográficas - FCT

Posicionamento relativo na região equatorial em diversas condições ionosféricas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de multicaminho de alta frequência no posicionamento relativo GPS estático: detecção e atenuação utilizando wavelets

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Simulação hidrodinâmica e caracterização experimental de mecanismos anti-sifão em sistemas de drenagem externa de líquido cefalorraquidiano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Investigações preliminares sobre a influência do clima espacial no posicionamento relativo com GNSS

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de modelos troposféricos no posicionamento baseado em redes usando o conceito de VRS

In the past few years several GPS (Global Position System) positioning techniques have been develope and/or improved with the goal of obtaining high accuracy and productivity in real time. The reference station network concept besides to enabling quality and reliability in positioning for scientific and civil GPS community, allows studies concerning tropospheric refraction modeling in the network region. Moreover, among the network corrections transmission methods available to users, there is the VRS (Virtual Reference Station) concept. In this method, the data of a virtual station are generated near the rover receiver (user). This provides a short baseline and the user has the possibility of using a single frequency receiver to accomplish the relative positioning. In this paper, the methodology applied to generate VRS data, using different tropospheric models is described. Thus, comparative tests were conducted in the four seasons with the NWP/INPE (Numerical Weather Prediction/National Institute for Space Research) and Hopfield tropospheric models. In order to analyse the VRS data quality, it was used the Precise Point Positioning (PPP) method, where satisfactory results were found. Mean differences between PNT/INPE and Hopfield models of 9.75% and 24.2% for the hydrostatic and wet days, respectively were obtained.

Análise de séries temporais de multicaminho em estações de monitoramento contínuo

Data from reference stations are widely used in GNSS (Global Navigation Satellite System) positioning, and can be used in relative positioning or network-based positioning concept. Positioning accuracy will be directly influenced by errors in signals collected in these stations. In this paper, it is aimed at evaluating these data quality using temporal series of multipath index MP1 and MP2. A statistical study of temporal series with 7 years of daily observations related to 7 stations from RBMC (Rede Brasileira de Monitoramento Contínuo) was accomplished. In order to investigate trends and seasonality a linear regression model, correlograms, and Fourier periodograms were used. We also used a harmonic adjust to identify peaks on temporal series. At last, the possible causes of seasonality found in some stations were discussed. It was also possible to identify peaks in MP values of March and October months (mainly in stations located near geomagnetic equator).

Novos peptídeos sintéticos e estudo da interação com membranas modelo: efeito de modificações no N-terminal na atividade lítica e antimicrobiana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Determinação das coordenadas dos vértices limítrofes de imóveis rurais utilizando-se do posicionamento GPS

Aiming to evaluate the methodologies of surveys related with the specifications of Geo-referencing Rural Parcels in Brazil, established by INCRA (National Institute of Colonization and Agrarian Reform), in attendance to the Law 10.267/01, and considering aspects of precision and accuracy, a test area was set up in the Assentamento Florestan Fernandes, in the county of Presidente Bernardes, SP. This area was subdivided in three sub-areas in order to simulate the existence of three contiguous rural parcels. The first stage of work consisted in the implantation and determination of the control points coordinates in their respective areas. These control points were determined by the process of direct transport, using dual frequency (L1/L2) GPS receivers and through the process of traverse with baselines of up to 20 km, using single frequency receivers (L1). The coordinates of the points of the perimeter of the three sub-areas were determined using single frequency GPS receivers, from two survey methods, both using static relative positioning. The first one, so called traverse, each point that delimits the property was occupied successively, starting from the control point and closing at another. In other, denominated double irradiation, each point of the property was irradiated from two control points. These procedures were accomplished with the intention to define and to implement a mistake control strategy, to realize redundancy measurements and to use an adjustment method correctly, to obtain trustworthily values in the patterns demanded in each kind of survey. With the data collected and processed some analyses could be accomplished from the discrepancies between the coordinates obtained by different methodologies. The results show that this test area may be used to validate other methodologies and equipments. The results were satisfactory and attended the specifications of Geo-referencing Rural Parcel.

Proximity-accelerated chemical coupling reaction in the benzodiazepine-binding site of gamma-aminobutyric acid type A receptors: superposition of different allosteric modulators

Benzodiazepines are widely used drugs. They exert sedative/hypnotic, anxiolytic, muscle relaxant, and anticonvulsant effects and act through a specific high affinity binding site on the major inhibitory neurotransmitter receptor, the gamma-aminobutyric acid type A (GABA(A)) receptor. Ligands of the benzodiazepine-binding site are classified into three groups depending on their mode of action: positive and negative allosteric modulators and antagonists. To rationally design ligands of the benzodiazepine site in different isoforms of the GABA(A) receptor, we need to understand the relative positioning and overlap of modulators of different allosteric properties. To solve these questions, we used a proximity-accelerated irreversible chemical coupling reaction. GABA(A) receptor residues thought to reside in the benzodiazepine-binding site were individually mutated to cysteine and combined with a cysteine-reactive benzodiazepine site ligand. Direct apposition of reaction partners is expected to lead to a covalent reaction. We describe here such a reaction of predominantly alpha(1)H101C and also three other mutants (alpha(1)G157C, alpha(1)V202C, and alpha(1)V211C) with an Imid-NCS derivative in which a reactive isothiocyanate group (-NCS) replaces the azide group (-N(3)) in the partial negative allosteric modulator Ro15-4513. Our results show four contact points of imidazobenzodiazepines with the receptor, alpha(1)H101C being shared by classical benzodiazepines. Taken together with previous data, a similar orientation of these ligands within the benzodiazepine-binding pocket may be proposed.

Structural flexibility in transcription complex formation revealed by protein–DNA photocrosslinking

The Oct-1 POU domain binds diverse DNA-sequence elements and forms a higher-order regulatory complex with the herpes simplex virus coregulator VP16. The POU domain contains two separate DNA-binding domains joined by a flexible linker. By protein–DNA photocrosslinking we show that the relative positioning of the two POU DNA-binding domains on DNA varies depending on the nature of the DNA target. On a single VP16-responsive element, the POU domain adopts multiple conformations. To determine the structure of the Oct-1 POU domain in a multiprotein complex with VP16, we allowed VP16 to interact with previously crosslinked POU-domain–DNA complexes and found that VP16 can associate with multiple POU-domain conformations. These results reveal the dynamic potential of a DNA-binding domain in directing transcriptional regulatory complex formation.

Controle cooperativo aplicado a sistemas de posicionamento dinâmico.

Hoje em dia com o crescente aumento da exploração de petróleo e gás em águas profundas, há um aumento na demanda por operações offshore envolvendo a cooperação entre unidades flutuantes. Tais operações requerem um alto nível de planejamento e coordenação, o que na maioria dos casos é feito com a troca de informação no nível de operação, com cada unidade flutuante comandada independentemente. Exemplos de operações deste tipo vão desde operações de alívio passando por operações de instalação de equipamento submarino, até operações de pesquisa envolvendo múltiplas unidades flutuantes dotadas de sistema de posicionamento dinâmico (DP). As vantagens do controle cooperativo surgem com a redução do erro da distância relativa durante a manutenção do posicionamento ou durante a execução de manobras de posicionamento conjuntas. No presente trabalho, os conceitos de controle de consenso são aplicados de forma combinada com o sistema DP de cada navio. A influência dos ganhos do controlador cooperativo no sistema como um todo será discutida, utilizando-se técnicas de análise da resposta em frequência. Simulações completas no domínio do tempo e experimentos usando modelos em escala serão utilizados para se demonstrar o funcionamento do controle cooperativo. Todas as simulações serão conduzidas no simulador Dynasim e os ensaios experimentais no tanque de provas da Engenharia Naval da Escola Politécnica da Universidade de São Paulo. Além disso, serão feitas comparações entre os experimentos em tanque de provas e simulações numéricas equivalentes, demonstrando-se a validade dos ensaios numéricos. Será também demonstrado que os requisitos de projetos adotados são atendidos pelos ensaios em tanque de provas. .