Eficacia del plasma rico en plaquetas en procedimientos médicos estéticos faciales . Evaluación en contorno de ojos.

Introducción: En la actualidad se están implementando nuevas técnicas, para el tratamiento de líneas de expresión facial. El Plasma Rico en Plaquetas (PRP) utiliza factores de crecimiento humano autólogos con fines médicos estéticos. El objetivo de este trabajo fue evaluar el tratamiento con plasma rico en plaquetas en el manejo del rejuvenecimiento periocular. Materiales y métodos: Estudio descriptivo retrospectivo en una cohorte de 27 pacientes entre 30 a 70 años de ambos sexos,tratados con PRP sin tratamientos médicos estéticos previos . Se compararon fotografías del sistema VISIA®, previo y posterior el PRP, para determinar los cambios del área periocular. Con análisis comparativo de medias utilizando pruebas t de student. Resultados: De 27 historias clínicas revisadas 96,3% eran mujeres, la edad promedio fue de 52.67 años. Se observaron cambios clínicos satisfactorios en el manejo del foto envejecimiento periocular con mejoría estadísticamente significativa entre el promedio inicial y el post tratamiento en arrugas, textura y porfirinas (p: 0.000). No se observaron diferencias estadísticamente significativas entre los grupos de edad (p = 0,62). Los pacientes analizados posterior al tratamiento se encuentran en mejor estado que el 63,78% de la población de su mismo sexo, edad y fototipo de piel. Los eventos adversos fueron disminuyendo en su frecuencia en cada una de las sesiones siguientes. Discusión: El PRP proporciona una mejoría global en los parámetros de envejecimiento periocular lo cual se correlaciono con los estudios previos in vitro. Conclusiones: El PRP es seguro y eficaz en contorno de ojos.

Difference in competence for in vitro proliferation and ex vitro growth of genetically identical mature and juvenile clones of apomictic Malus species

The apomictic system in Malus wits Used Is a model to examine rejuvenation by generating genetically identical tissue culture lines that had two entirely different developmental origins: either embryo-derived tissues (juvenile clones) or somatic tissue from the adult/mature tree (mature clones). These two lines were then subsequently used to examine in vitro difference between mature (M) and juvenile (J) tissues in potential for shoot, root proliferation and ex vitro (glasshouse) growth. The M clones of M. hupehensis and M. toringoides in vitro had significantly fewer total shoots and shoot more than 2 cm in length per proliferating explant than the J clones and also rooted less efficiently. Ex vitro (glasshouse) juvenile clones had shorter internodes, a greater number of leaves and more dry weight compared to their mature counterparts.

Morphology and myofiber composition of skeletal musculature of the forelimb in young and aged wild type and myostatin null mice

Most current research into therapeutic approaches to muscle diseases involves the use of the mouse as an experimental model. Furthermore, a major strategy to alleviate myopathic symptoms through enhancing muscle growth and regeneration is to inhibit the action of myostatin (Mstn), a transforming growth factor-beta (TGF-beta) family member that inhibits muscle growth. Presently, however, no study has expanded the morphological analysis of mouse skeletal muscle beyond a few individual muscles of the distal hindlimb, through which broad conclusions have been based. Therefore, we have initially undertaken an expansive analysis of the skeletal musculature of the mouse forelimb and highlighted the species-specific differences between equivalent muscles of the rat, another prominently used experimental model. Subsequently, we examined the musculature of the forelimb in both young and old adult wild-type (mstn(+/+)) and myostatin null (mstn(-/-)) mice and assessed the potential beneficial and detrimental effects of myostatin deletion on muscle morphology and composition during the aging process. We showed that: (1) the forelimb muscles of the mouse display a more glycolytic phenotype than those of the rat; (2) in the absence of myostatin, the induced myofiber hyperplasia, hypertrophy, and glycolytic conversion all occur in a muscle-specific manner; and, importantly, (3) the loss of myostatin significantly alters the dynamics of postnatal muscle growth and impairs age-related oxidative myofiber conversion.

Adult skeletal muscle stem cell migration is mediated by a blebbing/amoeboid mechanism

Adult skeletal muscle possesses a resident stem cell population called satellite cells which are responsible for tissue repair following damage. Satellite cell migration is crucial in promoting rapid tissue regeneration but is a poorly understood process. Furthermore, the mechanisms facilitating satellite cell movement have yet to be elucidated. Here the process of satellite cell migration has been investigated revealing that they undergo two distinct phases of movement; firstly under the basal lamina and then rapidly increasing their velocity when on the myofibre surface. Most significantly we show that satellite cells move using a highly dynamic blebbing based mechanism and not via lamellopodia mediated propulsion. We show that nitric oxide and non-canonical Wnt signalling pathways are necessary for regulating the formation of blebs and the migration of satellite cells. In summary, we propose that the formation of blebs and their necessity for satellite cell migration has significant implications in the future development of therapeutic regimes aimed at promoting skeletal muscle regeneration.

Adeno-associated virus-8-Mediated intravenous transfer of myostatin propeptide leads to systemic functional improvements of slow but not fast muscle

Myostatin is a member of the transformating growth factor-_ (TGF-_) superfamily of proteins and is produced almost exclusively in skeletal muscle tissue, where it is secreted and circulates as a serum protein. Myostatin acts as a negative regulator of muscle mass through the canonical SMAD2/3/4 signaling pathway. Naturally occurring myostatin mutants exhibit a ‘double muscling’ phenotype in which muscle mass is dramatically increased as a result of both hypertrophy and hyperplasia. Myostatin is naturally inhibited by its own propeptide; therefore, we assessed the impact of adeno associated virus-8 (AAV8) myostatin propeptide vectors when systemically introduced in MF-1 mice. We noted a significant systemic increase in muscle mass in both slow and fast muscle phenotypes, with no evidence of hyperplasia; however, the nuclei-to- cytoplasm ratio in all myofiber types was significantly reduced. An increase in muscle mass in slow (soleus) muscle led to an increase in force output; however, an increase in fast (extensor digitorum longus [EDL]) muscle mass did not increase force output. These results suggest that the use of gene therapeutic regimens of myostatin inhibition for age-related or disease-related muscle loss may have muscle-specific effects.

Mechanisms of motivation-cognition interaction: challenges and opportunities

There has been a recent rejuvenation of interest in studies of motivation-cognition interactions arising from many different areas of psychology and neuroscience. The current issue of Cognitive, Affective, and Behavioral Neuroscience provides a sampling of some of the latest research from a number of these different areas. In this introductory paper, we provide an overview of the current state of the field, in terms of key research developments and candidate neural mechanisms receiving focused investigation as potential sources of motivation-cognition interaction. However, our primary goal is conceptual: to highlight the distinct perspectives taken by different research areas in terms of how motivation is defined, the relevant dimensions and dissociations that are emphasized, and the theoretical questions being targeted. Together, these distinctions present both challenges and opportunities for efforts aiming towards a more unified and cross-disciplinary approach. We identify a set of pressing research questions calling out for this sort of cross-disciplinary approach, with the explicit goal of encouraging integrative and collaborative investigations directed towards them.

Aspectos morfo-tectônicos nos platôs de Portalegre, Martins e Santana/RN

A morpho-structural analysis was performed in the uplifted siliciclastic deposits of the Serra do Martins Formation along the Portalegre, Martins and Santana plateaux, in the southeastern and central regions of Rio Grande do Norte State. Due to the lack of biostratigraphic records, this formation has a disputable age.The adopted approach was based on the analysis of the drainage patterns and in the recognition of topographic surfaces and regional structures, subjected to neotectonic deformation and rejuvenation the present stress field. These events are recorded in the lineament arrays and as anomalous features of the landscape, such as the uplifted plateaux.The morpho-sculptural evolution of the studied blocks is expressed as erosive and accumulative processes. The former ones include erosional scarpments, cuestas and amphitheaters as the most characteristic features, while debris slopes represent acumulative examples. Such elements attest to the recent disequilibrium of the plateaux, and the absence of well developed alluvium terraces suggest an accelerated uplift process. The directions of the linear features observed in remote sensing products evidence the control of the basement structural trends, inherited from the pre-Cenozoic evolution. The NNE-SSW direction controls the main erosional features of the plateaux, while the N-S direction is a major drainage control, being also recognized in the Potiguar Basin. An E-W trend occurs as a less developed direction, reflecting either a system of mesozoic basic dykes or precambrian brittle structures. As regards to the drainage arrays, an arborescent, varying to a roughly N-S rectangular pattern, was identified in the Portalegre-Martins block. The Santana plateau displays rectilinear (northern border) and dendritic arborescent (southern border) patterns. In the sedimentary cover, the drainage pattern varies from rectangular to angular, reflecting inheritance from the crystaline basement. The most significative directions, N, NE and NW, mark the erosional fronts of the plateaux. Drainage anomalies, characterized by elbows or paralell confluencies, reinforce the arguments mentioned above. The data sets evidence the relationships between endogenous (lithology, structures) and exogenous features as the main controls of terrain dissecation, associated to vertical (epirogenesis) movements and horizontal tectonics. A final discussion addresses the relationships of the Serra do Martins Formation with the sedimentary record of Potiguar Basin, trying to establish chronostratigraphic links with the main evolutionary steps of this part of the Borborema Province, and possible mechanisms involved in the uplift of the plateaux and other stratigraphic units in the region

Durango apatite fission-track dating using length-based age corrections and neutron fluence measurements by natural thorium thin films and natural U-doped glasses calibrated through natural uranium thin films

In this work, we present an approach for neutron fluence measurements based on natural thorium thin films and natural uranium-doped glasses calibrated through natural uranium thin films to be used for dating with the Fission-Track Method (FTM). This neutron dosimetry approach allows the employment of FTM even when dating is carried out using low neutron themalization facilities. Besides, it makes possible the determination of the Th/U ratio of the mineral to be dated. Durango apatite which is often employed in FTM as an age standard was analyzed. This apatite presented a fairly high Th/U ratio, 29.9 +/- 1.7. Th fissions were 18%, 12% and 10% of the total for irradiations where thermal to fast neutron flux ratios were 2.4, 4.4 and 5,2, respectively. These results show that Th fission must be considered for this apatite, when not well-thermalized irradiation facilities are used. The ratio between spontaneous and induced track length, L(S)/L(1), close to 0.89, indicates a certain amount of rejuvenation of the age of Durango apatite. Therefore, its apparent age should be corrected, the application of a technique based on track-length measurements produced a corrected age of 29.7 +/- 1.1 Ma, consistent with the independent reference age of this apatite (31.4 +/- 0.5 Ma). This result represents a support for viability of the neutron dosimetry approach studied in this work for FTM.(C) 2002 Elsevier B.V. B.V. All rights reserved.

New treatment for crow's feet wrinkles by vertical myectomy of the lateral orbicularis oculi

Crow's feet wrinkles are common complaints in patients looking for facial rejuvenation. This article describes a new technique for a lasting treatment by vertical myectomy of the lateral orbicularis oculi muscle. The author presents 20 cases surgically treated in the past 16 months. The results are very promising.

The geotectonic environments of Early Precambrian granulites in Brazil

A review is presented concerning Archaean granulites occurring in some old domains of the South American Platform, which was consolidated at the end of the Brazilian Cycle (900-500 Ma). The rocks occur in different geotectonic environments and show variable ages, structures and lithological associations. The most important complexes are the Atlantic Granulite Belt in the São Francisco Craton and the Goias Granulite Belt in the Central Goias Massif, both several hundred kilometres long. The former is composed of the Caraibas Complex, the Jequié Complex, the Salvador Complex and several minor granulite occurrences along the Brazilian coast in the States of Espírito Santo and Rio de Janeiro. The latter includes the large basic-ultrabasic complexes of Barro Alto, Tocantins and Canabrava. Both belts consist of massive or foliated rocks, banded or homogeneous and varying from acidic to ultrabasic in composition. They are the result of metamorphism affecting diversified supra- and infracrustal material. The Atlantic Granulite Belt lies between greenstone/granite terrains which show ovoid and boomerang-type dome structures. The contacts between both are either tectonic or transitional. Another occurrence of Archaean granulites comprises intercalations of palaeosomes and melanosomes within migmatites and anatectic rocks. These vary in size from small lenses to irregular complexes which may attain sizes of several hundred square kilometres. Apart from migmatites, they are associated with gneisses, schists and granitoid bodies. They are located in regions which underwent remobilization of varying intensity during the Middle and Late Precambrian. The rocks show polymetamorphism, K-feldspar blastesis, tectonic overprinting and isotopic rejuvenation. These granulites are in some cases very similar to those formed during the Middle Precambrian. In some places it is therefore quite difficult to distinguish between Early and Middle Precambrian granulites - the more so, since interpretations of radiometric age values are largely controversial. At present there is no evidence of granulitic rocks related to the Late Precambrian geotectonic cycles of Brazil. © 1979.

Ácido glicólico: Agente despigmentante e rejuvenescedor

The skin pigmentation caused by ultraviolet light irradiation as a defense against the carcinogenic action of solar light may lead to early skin aging and to hyperchromia, which treatment requires the use of photo-protective, depigmenting and rejuvenating agents. Recently, there have been used many substances for the prevention and/or treatment of skin aging as well as to lowering the skin pigmentation. Glycolic acid is the alpha-hydroxy acid most commonly used in cosmetic and dermatological prepatations. This use is due to its depigmentating and rejuvenating properties and its efficacy at different concentrations, when incorporated to different kind of excipients.

Studying the satisfaction of patients on the outcome of an aesthetic dermatological filler treatment

Background: Many factors contribute to extend productive life in the modern world. Competition makes people worry about physical appearance, mosftly in respect to facial and skin aging. This has motivated new developments in cosmetic dermatology and the need of evaluating patient satisfaction with the new proposed treatments. Poll questionnaire has been used for such evaluation, and the analysis of the electroencephalogram (EEG) mapping obtained while the patient answers the satisfaction questionnaire may render the results less subjective. Objectives: The purpose of this paper is to study the satisfaction of a group of 33 women (mean age, 44.years) treated with hyaluronic acid filling of nasolabial folding or lips, combining the EEG brain mapping and questionnaire techniques. Methods: At the third month of evaluation, two networked personal computers were used for the EEG recording and for presenting the patient with a questionnaire about her well-being feeling; self-evaluation of her face; her satisfaction with the results of the aesthetic treatment; how the family, friends, and people at work evaluated the result of the treatment; and her decision to repeat the treatment and to recommend it to friends and family. Results: Poll results showed that patients were feeling well and were satisfied with the results of the aesthetic treatment. Furthermore, the regression EEG mappings showed patients to be satisfied with their appearance and with the treatment involving similar brain areas. Conclusion: Patients decided to undergo the treatment because they were already considering it (54%) or because they were dissatisfied with their lips or nasolabial folding (52%). The fact that the treatment was free of charge solidified the decision. Patients consider themselves as good-looking and they wanted to preserve such a condition. © 2008 Wiley Periodicals, Inc.

Autologous orbicularis muscle for filling facial foldsan experimental and clinical study

Purpose: To present a technique for filling facial folds by using autologous orbicularis oculi muscle, based on an experimental model. Methods: two studies are presented: (1) an experimental study using 15 albino guinea-pigs from which a strip of the sural triceps muscle was removed and implanted in the subcutaneous tissue of the dorsal area. The animals were sacrificed 7, 30 and 60 days after the implantation, and the material was histologically evaluated. And (2%) an interventional prospective clinical trial carried out on 20 patients referred to blepharoplasty surgery. They received autologous preseptal orbicularis muscle for filling facial folds. The results where evaluated by patients satisfaction and clinical exam. Results: the sural tricep muscle, when implanted in the subcutaneous tissue, resulted in fibrosis. The patients whom received autologous orbicularis muscle implanted for filling facial folds showed that the procedure can be successfully carried out. Conclusions: autologous preseptal orbicularis muscle is a good material for filling facial folds. Cicatricial tissue will be formed on its implantation site, filling the tissue gap that forms the folds on the skin.

Tourism life cycle, tourism competitiveness and upgrading strategies in the Caribbean

In the 1980s Butler adapted the life cycle product model to the tourism industry and created the “Tourism Area Life Cycle (TALC) model”. The model recognizes six stages in the tourism product life cycle: exploration, investment, development, consolidation, stagnation and followed, after stagnation, by decline or revitalization of the product. These six stages can in turn be regrouped into four main stages. The Butler model has been applied to more than 30 country cases with a wide degree of success. De Albuquerque and Mc Elroy (1992) applied the TALC model to 23 small Caribbean island States in the 1990s. Following De Albuquerque and Mc Elroy, the TALC is applied to the 32 member countries of the Caribbean Tourism Organization (CTO) (except for Cancun and Cozumel) to locate their positions along their tourism life-cycle in 2007. This is done using the following indicators: the evolution of the level, market share and growth rate of stay-over arrivals; the growth rate and market share of visitor expenditures per arrival and the tourism styles of the destinations, differentiating between ongoing mass tourism and niche marketing strategies and among upscale, mid-scale and low-scale destinations. Countries have pursued three broad classes of strategies over the last 15 years in order to move upward in their tourism life cycle and enhance their tourism competitiveness. There is first a strategy that continues to rely on mass-tourism to build on the comparative advantages of “sun, sand and sea”, scale economies, all-inclusive packages and large amounts of investment to move along in Stage 2 or Stage 3 (Cuba, Dominican Republic, Puerto Rico). There is a second strategy pursued mainly by very small islands that relies on developing specific niche markets to maintain tourism competitiveness through upgrading (Anguilla, Antigua and Barbuda, British Virgin Islands and Turks and Caicos), allowing them to move from Stage 2 to Stage 3 or Stage 3 to a rejuvenation stage. There is a third strategy that uses a mix of mass-tourism, niche marketing and quality upgrading either to emerge onto the intermediate stage (Trinidad and Tobago); avoid decline (Aruba, The Bahamas) or rejuvenate (Barbados, Jamaica and the United States Virgin Islands). There have been many success stories in Caribbean tourism competitiveness and further research should aim at empirically testing the determinants of tourism competitiveness for the region as a whole.

Políticas públicas: a construção de imagens e sentidos para o envelhecimento humano

The present study aims to analyze the Brazilian public policies made for the elderly citizen population and to examine their resonance in the production of senses and images on the process of aging. For this, we draw cartographies that could find producing figures of sense about the aging, passing through some politics formulated for to this age group, since the decade of 1920 until the creation of the “Estatuto do Idoso”, in 2003. We checked that the appearance of public policies for the old age happened as an answer to the challenge of managing and controlling the aging process courses in front of the increase of the elderly citizen population. The “Estatuto do Idoso” is an important landmark of the management advance of the State upon the oldness and of the substitution of an philanthropic-assistance model for a preventive model which is based on the promotion of a healthy and productive longevity. With that, next to the figure of the sick and inactive elderly , it begins to appear figurations of a healthy and powerful aging, crossed by images of vitality and rejuvenation.