Sidi Hammou ; Sidi Hammou / Rais El Mokhtar Ben Said, chant [acc. voc. et instr.]

[Traditions. Afrique du Nord. Maroc]

Le Maadeur ; M'Touga / Rais El Mokhtar Ben Said, chant [acc. voc. et instr.]

[Traditions. Afrique du Nord. Maroc]

Development of Elispot and CFSE flow cytometric assays for detecting beryllium sensitivity

Background: Beryllium sensitization (BeS) is caused by exposure to beryllium in the workplace and may progress to chronic beryllium disease (CBD). This granulomatous lung disorder mimicks sarcoidosis clinically, but is characterized by beryllium specific CD4+ T-cells immune response. BeS is classically detected by beryllium lymphocyte proliferation test (BeLPT), but this assay requires radioactivity and is not very sensitive. In the context of a study aiming to evaluate if CBD patients are misdiagnosed as sarcoidosis patients in Switzerland, we developed EliSpot and CFSE beryllium flow cytometric test. Methods: 23 patients considered as having sarcoidosis (n = 21), CBD (n = 1) and possible CBD (n = 1) were enrolled. Elispot was performed using plate covered with gamma-IFN mAb. Cells were added to wells and incubated overnight at 37 °C with medium (neg ctrl), SEB (pos ctrl) or BeSO4 at 1, 10 and 100 microM. Anti-IFN-gamma biotinylated mAb were added and spots were visualized using streptavidinhorseradish peroxidase and AEC substrate reagent. Results were reported as spot forming unit (SFU). For Beryllium specific CFSE flow cytometry analysis, CFSE labelled cells were cultured in the presence of SEB and 1, 10 or 100 microM BeSO4. Unstimulated CFSE labeled cells were defined as controls. The cells were incubated for 6 days at 37 °C and 5% CO2. Surface labelling of T-lymphocytes and vivid as control of cells viability was performed at the time of harvest. Results: Using EliSpot technology, we were able to detect a BeS in 1/23 enrolled patients with a mean of 780 SFU (cut off value at 50 SFU). This positive result was confirmed using different concentration of BeSO4. Among the 23 patients tested, 22 showed negative results with EliSpot. Using CFSE flow cytometry, 1/7 tested patients showed a positive result with a beryllium specific CD4+ count around 30% versus 45% for SEB stimulation as positif control and 0.6 % for negative control. This patient was the one with a positive EliSpot assay. Conclusions: The preliminary data demonstrated the feasibility of Elispot and CFSE flow cytometry to detect BeS. The patient with a beryllium specific positive EliSpot and CFSE flow cytometry result had been exposed to beryllium at her workplace 20 years ago and is still regularly controlled for her pulmonary status. A positive BeLPT had already been described in 2001 in France for this patient. Further validation of these techniques are in progress.

Tassanou Igan Ouzzal ; Chant de Tiznit / Rais Abdallah Enair, chant [acc. instr.]

[Traditions. Afrique du Nord. Maroc]

Comorbiditat de la síndrome de Gilles de la Tourette i la síndrome d’Asperger : a propòsit d’un cas

Presentem una anàlisi qualitativa sobre l’evolució de la simptomatologia i la intervenció en àmbit escolar d’un adolescent de 15 anys diagnosticat de síndrome de Gilles de la Tourette i de síndrome d’Asperger. En aquests dos trastorns la comorbiditat no ha estat prou investigada. Realitzem una observació directa del comportament de l’adolescent en l’ambient escolar durant dos trimestres. S’utilitza una agenda del dia a dia acadèmic on s’apunten totes les dades rellevants referents a la conducta del subjecte, recull de dibuixos o narracions personals amb la finalitat de confeccionar un llistat de les conductes patró. Amb l’objectiu d’augmentar el nivell d’autoconsciència del subjecte en quant al control i descontrol de les seves conductes, se li aplica: reforç positiu permanent i contenció en situacions amb conductes més violentes. Observem que, independentment del diferent origen psicogenètic i psiconeurològic d’ambdues síndromes, les conductes resultants son similars, i que la intervenció aplicada dóna uns resultats favorables. Els resultats ens porten a afirmar que en aquest cas la intervenció cognitiu- conductual esdevé més eficaç que la intervenció conductual.

Élie de Saint Gilles : chanson de geste / publ. avec introd. par Gaston Raynaud ; trad. par Eugène Koelbing

[Élie de Saint-Gilles (français ancien). 1879]

Élie de Saint Gilles : chanson de geste / publ. avec introd. par Gaston Raynaud ; trad. par Eugène Koelbing

[Élie de Saint-Gilles (français ancien). 1879]