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A study on the effects of artificial feeds on the growth and production of fishes in polyculture in 6 ponds along with some limnological conditions was conducted. Species of Indian and Chinese major carps (Labeo rohita, Catla catla, Cirrhinus mrigala, Hypophthalmicthys molitrix) and catfishes (Clarias batrachus, Clarias gariepinus) were stocked in 6 ponds. Stocking rate in both cases were 32044 fingerlings per hectare. Ratio of species of Rui:Catla:Mrigal:Silver carp:African Magur:Local Magur=25%:25%:5%:25%:14%:6%. Fertilization and artificial feeds were given in 3 ponds (treatment I) and only fertilization was done in other 3 ponds (treatment II). Average yield/ha/yr was 7.903 m.ton in case of fertilization and artificial feeding application and 3.374 m.ton in case of only fertilization application. Urea, TSP and cow dung were applied fortnightly at the rates of 400 kg/ha/yr, 2000 kg/ha/yr and 4000 kg/ha/yr respectively. Wheat bran, rice bran and mustard oil cake were given daily as an artificial feed in treatment I. Whereas treatment II was conducted without any artificial feed. Ratio of artificial feed was wheat bran:rice bran:oil cake=2:2:1 (by wt). Absence of artificial feed in 3 ponds under treatment II seriously affected the growth and production of fish.

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An experiment was conducted to study the effect of different feeds on growth survival and production of African catfish (C. gariepinus) in six cemented tanks (3m² each) over a period of 120 days. Three different feeds namely Feed A (Saudi-Bangla fish feed, 33.43% protein), Feed B (formulated feed, 40.12% protein) and Feed C (chicken raw intestine, 59.58% protein) were applied to treatments I, II and III respectively. Each of the tanks was stocked with 24 fry with mean initial body weight of 2.56±0.06 g. Feeds were supplied to the fish ad-libitum daily in two instalments. Significantly highest weight gain was obtained in treatment III, however, survival rate was low compared to other treatments. The feed conversion ratio (FCR) values ranged from 2.52-6.4. Survival rate of fish varied between 83 and 96%. Treatment II yielded the highest (5000 kg/ha/120 days) production with the highest survival rate of fish. On the basis of survival rate and production, it is suggested that the formulated feed (Feed B) is suitable for the culture of C. gariepinus in cemented tanks.

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Laboratory experiments were conducted to determine the effect of compounded feeds on growth of juveniles (3 to 4 cm) of the Banana prawn Penaeus merguiensis. In all, seven diets were prepared with different combinations of locally available ingredients. Diascure, a local tuber was used as source of carbohydrate and vitamin mineral mixture (1%) was used in all the diets. The results indicated that diets with groundnut cake (diet 2, 3, 5 and 7) produced better survival and growth. In diets 4 and 6 growth was good but survival was very poor. Amongst all the diets, diet 7 produced significantly better growth, survival and food conversion ratio.

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A nutritional study was carried out to know the feasibility of formulated and commercial feeds on the growth of juveniles of the freshwater prawn Macrobrachium malcolmsonii. Best relative growth rate was observed for feed 1 followed by feeds 2 and 3. Animals fed with feed 1 showed higher production than the other feeds. Higher assimilation efficiency was noticed in the animals provided with feeds 5 and 4. Higher gross and net growth efficiencies were observed in the animals offered feed 1. Among the commercial feeds, feed 3 may considered to be suitable alternative to feed 1.

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Effect of different feeds and salinities on cyst production in Artemia sp. was studied. Among the 12 different feeds used, ragi powder suspension showed the best growth and cyst production. A wide range of salinity from 90 to 210‰ was used to study its effect on cyst production. Among all these treatments, 130‰ salinity was found suitable for induction of oviparity and cyst production in the San Francisco Bay strain of Artemia sp.

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The present communication is a survey report carried out to assess the incidence of toxic mycoflora on seven types of agriculture products/by products incorporated during fish culture as supplementary dietary items. Samples were obtained from various sources at Darbhanga, Madhubani and Samashtipur districts during summer, winter and monsoon months. Out of the total 1774 samples, only 894 appeared to be fresh visually reflecting average incidence of contamination around 46.6%. However, the apparently fresh samples, when subjected to culture, 26.9% of them were found to be contaminated. Thus, degree of fungal spoilage in feed ingredients in parts of north Bihar appears to be significantly high (73.5%). The present study illustrates the facts with special reference to Aspergillus flavus, A. parasiticus (elaborating aflatoxins) A. ochraceous, Penicilium viradicatuin (elaborating ochratoxins) and A. versicolor (elaborating sterigmatocystin). The other strains already known for their toxigenic potentials that appeared on the present substrates included A. niger, A. fumigatus, A. candidus, P. islandicum, Rhizopus spp. and Mucur spp. Studies indicate that the prevalent climatic factors like temperature and relative humidity facilitate a congenial condition almost all through the year and in particular during summer and monsoon months. But water content of the substrates is a vital factor that further accelerates the pace of mycobial spoilage. A thorough sun-drying of the agricultural commodities before prolonged storage to bring water content below the "low risk limit" may significantly reduce the incidence of molds.

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Experiment was conducted to study the effect of dietary protein level on growth and nutrient utilization by angel fish (Pterophyllum scalare) juveniles. Fifty-four juveniles (average wt. 2-2.5 g) were equally divided in three treatments with each of three replicates. Three formulated diets with graded protein levels, T1 (35% CP), T2 (40% CP) and T3 (45% CP) were fed to juveniles for 45 days. A trend of higher weight gain %, SGR, FER and PER was found with the increased CP level in the feed. Feed intake was similar in all the groups. T3 group fed with 45% CP registered highest weight gain % (43.26 ± 2.07), SGR (0.78 ± 0.04), FER (0.29 ± 0.01), which were significantly higher (P<0.05) than the T1 and T2 groups. Protein digestibility of T2 and T3 groups was significantly higher than the T1 group. Survival was similar in all the experimental groups. Diet with 45% CP with protein energy ratio of 112.62 mg protein/K cal. is ideal for juvenile angel fish for indoor rearing.

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Studies were conducted to observe the effects of different types of feeds on the gonado-somatic index (GSI) and fecundity of freshwater prawn Macrobrachium rogenbergii. Three different treatments (T1 T2 and T3) were designed with three types of feed as follows: (i) Saudi-Bangla Prawn feed 100% - T1 (ii) SaudiBangla prawn feed 50%+ local feed 50%- T2 and (iii) local feed 100%- T3. The results showed that the average value of gonado-somatic index (GSI) was 14.39, 14.35 and 14.36 and the average fecundity of M. rogenbergiiwas 99,741, 98,125 and 97,911 in T1 T2 and T3 respectively. No significant difference (p>00.5) was between gonado-somatic indices (GSI) and fecundities of M. rogenbergii among different feeding trails. The price of Saudi-Bangla prawn feed was very high (Tk. 23/kg) than the local feed (Tk. 14/kg). So, use of local feed was recommended for M. rogenbergii brood rearing.

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A 70 day long experiment was carried out to evaluate three commercial pangas feeds available in Bangladesh viz. Quality Feeds Ltd. (QF), Aftab Bohumukhi Farm Ltd. (ABF) and Saudi-Bangla Fish Feed Ltd. (SBFF) (designated as treatment_T1 T2 and T3) on the growth of Pangas, Pangasius hypophthalmus. Each treatment had two replicates using six experimental ponds of each 80m2 size. The stocking density was 250 fish/80m2. At the beginning, the fish were fed twice daily at 15% of their body weight which was gradually reduced to 10 and 6%, respectively for the rest of the period. The ranges of water quality parameters observed were: temperature 29.0°C- ~5.1 °C, pH 6.85 - 8.52, DO 1.71 - 7.65 mg/1 and transparency or Secchi depth 14.5 - 30.0 em. The mean weight gain of fish was significantly (~<0.05) higher in T3 followed by T2 and T. The specific growth rate (SGR) ranged. between 4.09 and 5.06, feed conversion ratio (FCR) values between 1.54 and 1.61 with treatment T1 showing the lowest FCR. Protein efficiency ratio (PER) values ranged between 2.03 and 2.11. The survival of fish varied between 90.4 and 91.6%. The significantly (P<0.05) highest production of fish (kg/ha/70 days) and profit (Tk/ha/70 days) was observe in T3 (SBFF) followed by T2 (ABF) and T1 (QF), respectively. The result of the study showed that on the basis of nutritive value and growth performance of pangas, feed from Saudi-Bangla Fish Feed Ltd. is the best.

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A study was conducted to evaluate the effects of feed made from locally available ingredients on polyculture of shrimp and three brackishwater finfish species. Hatchery produced post-larvae (PL) of shrimp Penaeus monodon (0.005g) were stocked at the rate of 15,000 PLs/ha. Brackishwater finfish species Liza parsia, Mugil cephalus and Rhinomugil corsula of 0.63-1.4lg collected from local rivers were stocked at the rate 8,000, 1,000 and 2,000/ha, respectively in four treatments. Shrimp and finfishes were fed four different experimental diets composed of fish meal, mustard oil cake, rice bran, oyster shell power and vitamin premixes at the rate of 3-5% estimated crop/day for 195 days. Among four treatments, P. monodon showed comparative better growth in T4 and T3. Finfish L. parsia showed its better performance in treatment T2. Species M. cephalus and R. corsula showed insignificant production. P. monodon showed better growth with diet of fish meal and mustard oil cake@ 28.84 and 33.65%, respectively in T 3 and 19.22 and 43.27%, respectively in treatment T4.

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Three dry pelleted feeds incorporating fish meal, fish silage or a mixture of colocasia leaf powder and fish meal were formulated for use in carp culture. The diets formulated were tested for water stability and also for changes in their quality parameters over storage of three months. The different pellets showed satisfactory water stability. The variations recorded in the proximate composition during the period of storage did not bring about any drastic change in the overall keeping quality of the feeds. Therefore, the three formulated feeds are considered suitable for use in the culture of carps.

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The culture of Penaeus monodon has explicitly defined the need for diet formulations or supplementary feeds that would promote optimum growth and survival of the animal. A total of 28 feed combinations were developed for P. monodon. Fish meal, shrimp head meal, squid head meal, Ascetes spp. rice bran, and soybean cake were used as primary ingredients in these feeds. The commercial vitamin mix No. 22 was added to the dry ingredients. Gelatinized corn starch and wheat flour were used as binders. The pellets were extruded using a portable kitchen grinder with a diameter of 4 mm. The products were either sun-dried for 8 hours or oven-dried overnight at 50 degree C to stabilize moisture at 8-10%. The pellets were then kept in covered glass bottles and stored in the laboratory at room temperature. The cost of the feeds excluding labour were also computed. The pellets were analyzed for protein, fat, carbohydrate, crude fiber, ash, and moisture contents using standard procedures. They were also analyzed for water stability. To test the stability of pellets in water, 2-g samples were placed in plankton nets (mesh #40) and suspended in water for two, and six hours. The undissolved samples were then vacuum-dried and the moisture determined. Cost of the feeds ranged from P1.10 to P2.60 per kg depending on the feed ingredient. Squid and Ascetes spp. were rather expensive for use as basic ingredients. Proximate analysis of dry weight showed percentage protein content ranged from 20-63 g; fat, 8-20 g; carbohydrate (by difference), 11-36 g; ash, 8-28 g; moisture, 6-11 g; and crude fiber, 5 . 13 g. Stability tests showed that after two hours, 35-88% of solids remained intact and after 6 hours, 20-55% of the pellets remained undissolved. When a pellet disintegrates easily, pollution of the water occurs. Chances for the shrimp to feed on the pellet is minimized when the pellet is unstable. Thus, the search for a more compact feed pellet has to be continued.

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Rice bran is widely used by fish farmers as supplementary feed while soybean cake is used both as feed and as fertilizer in fishponds. Both fish meal and shrimp head have been found acceptable as feed ingredients. However, not much is known of the acceptability and efficiency of a mixture of these ingredients as feed for Penaeus monodon larvae. Ninety 127-day old P. monodon were measured for length and weight and were randomly divided into nine aquaria each containing 20 liters of water. These were fed 'lampirong' for two months previous to the study. There were three replications for each treatment. Length, weight, and survival rates were used to compare the efficiency of the diets. Weighed amounts of pellets equivalent to 100% of the body weight were fed during the first three days and reduced to 50% thereafter. A stopwatch was used to determine the length of time that elapsed before the shrimps would approach the pellet. Ten shrimps approximately 4 months in age were placed in 10 liters of water in a 25-liter aquarium. Two grams of each pellet type were placed simultaneously on opposite sides of the aquarium. The time that elapsed from the moment the pellets sunk to the bottom up to the time that any one shrimp approached the pellets was recorded. The group fed the imported pellets gained the most. Those fed FP-2s-77 elongated faster than those fed FP-1s-77. Survival rate of those fed FP-2s-77 was 37% while those fed imported pellets was 73%. Both 1s and 2s pellets disintegrated in water easily but the imported pellets were stable even after six hours in water. The attractability test for the pellets showed that the prawns were more readily attracted to the pellets 1s and 2s than to the imported pellets.

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Four dry pelleted feeds containing 20%, 30%, 40% and 45% protein were formulated incorporating casein as the main source of protein for use in carp nutrition studies. The caloric content in all the feeds was maintained constant. The method of processing is described. The formulated diets were tested for water stability. This test has revealed that the diet containing 20%, 30% and 40% protein had better stability than that containing 45% protein. This was due to the relatively higher fat content in the former three diets. However, all the feeds were sufficiently stable at the end of one hour in which time carps are known to utilise supplementary diets.

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Growth and survival of P. japonicus postlarvae was studied using Tapes philippinarum and commercial formula feeds. After a 28-day feeding period, the highest survival rate of 100% was obtained in prawns receiving Diet-B, followed by those on Tapes Kyowa Hakko shrimp meal with survival rates of 80 and 76.7%, respectively. The lowest survival rate of 48.3% was obtained among postlarvae fed with squid meal. In this batch cannibalism was observed which resulted in low survival. Growth was highest in the postlarvae fed with Kyowa Hakko and mysid feeds. Diet-B gave a slightly lower weight increase but gave similar gain in length. The composition of the artificial diet, Diet-B, and growth and survival rates are tabulated.