Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.

Production of mexican brown macroalgae fucoidan and fucosidases under an integral green technology bioproceses by the biorefinery concept

Marine ecosystem can be considered a rather exploited source of natural substances with enormous bioactive potential. In Mexico macro-algae study remain forgotten for research and economic purposes besides the high amount of this resource along the west and east coast. For that reason the Bioferinery Group of the Autonomous University of Coahuila, have been studying the biorefinery concept in order to recover high value byproducts of Mexican brown macro-algae including polysaccharides and enzymes to be applied in food, pharmaceutical and energy industry. Brown macroalgae are an important source of fucoidan, alginate and laminarin which comprise a complex group of macromolecules with a wide range of important biological properties such as anticoagulant, antioxidant, antitumoral and antiviral and also as rich source of fermentable sugars for enzymes production. Additionally, specific enzymes able to degrade algae matrix (fucosidases, sulfatases, aliginases, etc) are important tools to establish structural characteristics and biological functions of these polysaccharides. The aims of the present work were the integral study of bioprocess for macroalgae biomass exploitation by the use of green technologies as hydrothermal extraction and solid state fermentation in order to produce polysaccharides and enzymes (fucoidan and fucoidan hydrolytic enzymes). This work comprises the use of the different bioprocess phases in order to produce high value products with lower time and wastes.

Hawaiian Pittospora. Some Mexican Coreopsideae. A note on Xylosma hawaiiense Seem. By Earl Edward Sherff.

v.22:no.10(1942)

Hawaiian Pittospora. Some Mexican Coreopsideae. A note on Xylosma hawaiiense Seem. By Earl Edward Sherff.

v.22:no.10:index(1942)

A list of a collection of Mexican mammals with descriptions of some apparently new forms, by D. G. Elliot.

v.3:no.8(1903)

Use of tobacco in Mexico and South America / by J. Alden Mason, Formerly Assistant Curator of Mexican and South American Archaeology.

no.16(1924)

A correlation of the Mayan and European calendars / by J. Eric Thompson, Assistant Curator of Mexican and South American Archaeology ; Berthold Laufer, Curator of Anthropology, editor.

v.17:no.1(1927)

Systematics and distribution of the Mexican and Central American rainfrogs of the Eleutherodactylus gollmeri group (Amphibia: Leptodactylidae) : a contribution in celebration of the distinguished scholarship of Robert F. Inger on the occasion of his sixty-fifth brithday / Jay M. Savage.

n.s. no.33(1987)

The Mexican and Central American lizards of the genus Sceloporus, by Hobart M. Smith.

v.26(1939)

Notes on Central American and Mexican coral snakes, by Karl P. Schmidt.

v.20:no.20(1936)

An evaluation of Seth E. Meek's contributions to Mexican ichthyology / Robert Rush Miller.

v.69:no.1(1976)

Notes on Mexican reptiles and amphibians, by Hobart M. Smith.

v.24:no.4(1939)

The civilization of the Mayas / by J. Eric Thompson, Assistant Curator of Mexican and South American Archaeology.

no.25(1927)

Some rare or little-known Mexican coral snakes.

v.39:no.19(1958)

The natural history of Mexican rattlesnakes / by Barry L. Armstrong and James B. Murphy.

no.5 (1979)