982 resultados para Pregnancy diagnosis


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Pós-graduação em Zootecnia - FMVZ

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Cirurgia Veterinária - FCAV

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The aim of this study was to analyze the effect of flushing on the reproductive performance of Morada Nova (MN) and Santa Inês (SI) ewes submitted to fixed time artificial insemination (TAI). Twenty seven SI and 24 MN supplemented with concentrate (1% of live weight, on average), for 75 days during the breeding season. After 30 days of supplementation, ewes were synchronized with the aid of a hormonal protocol (HP) based on progesterone, eCG and cloprostenol. The estrus observation was conducted at 12, 24, 36 and 48 h after the end of HP with the aid of two ruffians. TAI was done 55 h after the end of HP. From 20 to 45 days after the beginning of the HP ewes were exposed to rams (natural breeding). The pregnancy diagnosis was evaluated 70 days after TAI. We analyzed the weight, body condition score, estrus rate, pregnancy rate and prolificacy testing the effects of race, week of supplementation and body condition score class. The weight and body conditions of ewes varied according to the week of supplementation, with higher values in the first two weeks following TAI. The estrus rate was 88.2% and 43.2% of the ewes showed estrus up to 24 hours of the end of the HP. The pregnancy rate per TAI was 31.3% and the pregnancy rate after natural breeding was 50.0%. It was observed that body condition score classes interfered in pregnancy rates. There was a higher percentage of multiple births by pregnancy by TAI than by natural breeding. It was concluded that the flushing resulted in weight gain and better body conditions ensuring the standardization of the herd for breeding season, which therefore improved reproductive performance. The HP used advanced the onset of estrus and increased prolificacy, but was inefficient in the synchronization of woolless sheep.

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The objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.

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The present study aims to report ovum pickup (OPU), in vitro embryo production (IVEP) and embryo transfer (ET) outcomes of fresh and vitrified buffalo embryos. For this purpose, 36 buffalo donors were submitted to 11 OPU sessions (n = 201). A total of 998 oocytes (5.0 +/- 0.5/donor/session) and 584 viable oocytes (2.9 +/- 0.3/donor/session) were recovered. Viable oocytes (grades 1, 2 and 3) were subjected to IVM, IVF (D0) and IVC. On D2, 54.5% of cleavage rate was obtained. Embryo yield on D7 was 44.9% (grade 1: 229 embryos, grade 2: 5 embryos and grade 3: 28 embryos). From this total, 115 fresh (grades 1 to 3) and 70 vitrified embryos (only grade 1) were transferred into recipients previously synchronized with fixed time embryo transfer (FTET) protocol. Vitrification was performed using the cryotop method. Pregnancy diagnosis in fresh and in vitrified groups were, respectively: 43.5% (50/115) and 37.1% (26/70) on 30 days after embryo transfer, and 41.7% (48/115) and 31.4% (22/70) on 60 days after embryo transfer. In conclusion, our results demonstrate the possibilities for commercial use of the techniques of OPU, IVEP and ET of fresh and vitrified embryos in buffaloes.

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The modernization of image equipaments associate to the veterinary professionals qualification allowed an advance in diagnosis veterinary medicine, and, consequently, in premature pregnancy diagnosis in bitches. Currently, owners and creators search for information related to pregnancy detection, fetal development and viability and litter size determination. Through diagnosis methods as radiology and ultrasonography, associated to clinics aspects, the diagnosis of early pregnancy become more acurated and precise, allowing more quality in the accompaniment of prenatal of these bitches. Ultrasonographic exams help the accompaniment of these pregnancies through of visuals recourses, which can supply information related to pregnancy detection, embrionary and fetal development, fetal viability e litter size determination through fetals mensurations. The radiographic study has as principal indication the fetal counting, and has been the most acurated for this analyses. It may be done after the da s after the luteini ing hormone surge, when occurs the fetal mineralization. Due to its importance in the present time and in small animals clinic, the objective of this study is to discuss the main radiographics and ultrassonographics aspects of the diagnosis of pregnancy in bitches

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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OBJETIVO: Caracterizar as indicações das gestantes que procuraram o serviço de Medicina Fetal do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo para realização de procedimentos invasivos diagnósticos e avaliar os resultados dos cariótipos fetais e de suas gestações. MÉTODOS: Estudo observacional retrospectivo das gestantes que realizaram biópsia de vilo corial (BVC), amniocentese e cordocentese no período de fevereiro de 2005 a dezembro de 2009. Não foram incluídos outros procedimentos diagnósticos ou procedimentos terapêuticos. O resultado da gestação foi obtido através de consulta de prontuário eletrônico e/ou físico e/ou contato telefônico. RESULTADOS: Foram realizados 713 procedimentos (113 BVC, 340 amniocenteses e 260 cordocenteses). A principal indicação para a realização dos procedimentos invasivos foi a presença de alterações estruturais nos fetos, seguido por valores aumentados da translucência nucal e pela idade materna avançada. O cariótipo fetal esteve alterado em 186 casos (26,1%). A trissomia do cromossomo 18 foi a aneuploidia mais comum, seguida pela trissomia do 21, a monossomia do X e a trissomia do cromossomo 13. Ocorreram 4,9% de abortamento, 25,7% de natimortos e 13% de neomortos. Oito gestantes optaram pela interrupção judicial, e 99% das gestantes cujos fetos não apresentavam malformação e que apresentavam cariótipo fetal normal tiveram nativivos.