Larval development of estuary perch (Macquaria colonorum) and Australian bass (M. novemaculeata) (Perciformes: Percichthyidae), and comments on their life history

Morphological development of the larvae and small juveniles of estuary perch (Macquaria colonorum) (17 specimens, 4.8−13.5 mm body length) and Australian bass (M. novemaculeata) (38 specimens, 3.3−14.1 mm) (Family Percichthyidae) is described from channel-net and beach-seine collections of both species, and from reared larvae of M. novemaculeata. The larvae of both are characterized by having 24−25 myomeres, a large triangular gut (54−67% of BL) in postflexion larvae, small spines on the preopercle and interopercle, a smooth supraocular ridge, a small to moderate gap between the anus and the origin of the anal fin, and distinctive pigment patterns. The two species can be distinguished most easily by the different distribution of their melanophores. The adults spawn in estuaries and larvae are presumed to remain in estuaries before migrating to adult freshwater habitat. However, larvae of both species were collected as they entered a central New South Wales estuary from the ocean on flood tides; such transport may have consequences for the dispersal of larvae among estuaries. Larval morphology and published genetic evidence supports a reconsideration of the generic arrangement of the four species currently placed in the genus Macquaria.

Record catch of rock perch, Epinephelus fasciatus by bottom trawling off Ratnagiri

A productive fishing ground for rock perch (Epinephelus fasciatus) was located off Ratnagiri between 17 degree 00' to 17 degree 10' N latitude and 72 degree 20' to 72 degree 30' E longitude, around a depth of 96m during November, 1983. An average catch of 2 ton/hr. was recorded. Length frequency studies of E. fasciatus indicate the probability of a nursery ground consisting of three brood stocks. Hydrographic studies of the ground revealed that the bottom water was oxygen deficient below a strong thermocline extending from 15-75 m depth with the recorded bottom temperature amounting to 20.5 degree C.

Management of the nile perch, Lates niloticus, fishery in Lake Victoria in light of the changes in its life history characteristics

Nile perch, Lates niloticus Linnaeus, 1758, is a predatory fish of high commercial and recreational value. It can grow to a length of 2 m and a weight of 200 kg. In Uganda, Nile perch was originally found only in Lake Albert and the River Nile below Murchison Falls. The species is, however, widely distributed in Africa, occurring in the Nile system below Murchison Falls, the Congo, Niger, Volta, Senegal and in Lakes Chad and Turkana (Greenwood 1966).

Growth performance and morphological variations of local and Thai climbing perch (Anabas testudineus, Bloch)

Climbing perch locally known as koi (Anabas testudineus) is a popular food fish in our country. Thai climbing perch was introduced in Bangladesh from Thailand. To explore the variation in growth performance and orphological features of local and Thai climbing perch a study was undertaken. The highest gain in length, weight and SGR were found in Thai koi 12.23±0.38 cm, 55.83±0.53 g and 7.92±0.11 %/day respectively. Fourteen morphometric characters were studied where eleven (TL, SL, HL, HBD, LBD, DFL, PECFL, PELFL, AFL, UJL and LJL) showed significant difference (p<0.01) in Thai koi from the local ones. Of the meristic characters no. of dorsal fin rays (hard), anal fin rays (hard), caudal fin rays and scale along lateral line (upper and lower) as recorded from the Thai koi were significantly higher (p<0.01) than that of the local koi. The no. of dorsal fin rays (soft) in Thai koi were also significantly higher (p<0.05) from that of local koi. The number of vertebra were also variable in local and Thai climbing perch (25 in case of local koi and 26 in case of Thai koi). Hence, the results obtained form the present study satisfy the characteristics of A. testudineus which reveals that both the local and Thai koi belongs to the same species. Growth performance of Thai koi was better compared to local koi reared in same conditions.

Monoculture of climbing perch, Thai koi, Anabas testudineus (Bloch) under different stocking densities at on-farm

An on-farm trial was undertaken in nine earthen ponds (400 to 640m2) to evaluate the growth and production potentials of climbing perch, Thai koi (Anabas testudineus) for the period of four months from March through June 2006. Three stocking densities such as 75,000 (T1), 100,500 (T2) and 125,000/ha (T3) were tested with three replications. Fish were fed with commercial (SABINCO) pelleted feed (35% crude protein). Fish productions obtained in three treatments such as T1, T2 and T3 were 6,480±217, 6,384±154, 6,617±187 kg· 1ha, respectively. The results demonstrated higher mean growth in T1 than in T2 and T3. However, the net benefit was achieved Tk. 7,26,975 from T1, where the stocking density was 75,000/ha.

Predatory behaviour of a perch, Nandus nandus (Ham.)

Predatory behaviour of Nandus nandus was studied by offering Cyprinus carpio as prey. The study was conducted with six N. namdus (8.2 ±0.2 cm and 7.60 ±0.3g) represented as P 1, P 2, P 3, P 4, P 5 and P 6. Three size categories of prey (C. carpio) such as small (2.0 ±0.1 cm and 0.23 ±0.01g), large (3.6 ±0.1 cm and 0.57 ±O.O.lg) and mixed group consisting of both small and large prey were used for 14 days of trial. Predatory behavior was classified as targeting, driving, catching, handling, resting and next attempt of catching prey. After introduction of prey into the aquarium predators followed the movement of preys by eye movements and tried to target smaller one first. The predator grasped the head of the prey by its jaws by a drive and engulfed it wholly into the mouth. The average handling time (time taken to manipulate and swallow prey from capture to ceasation of pharyngeal movement) was 42±2 sec and 47±2 sec for small and large prey respectively. N. nandus were ingested more small prey than large prey though the size classes were equally available in case of mixed prey used. Although the prey consumption was higher in number when small prey were ingested but in weight the consumption was higher when ingested large size of prey. The study indicated that N. nandus, ingested more small prey and grasped the headfirst.

Seed production and culture techniques of climbing perch, Anabas testudineus

Six treatments each with 12 replications designed to optimize the dose of inducing agent PG to achieve fertilization and hatching success of climbing perch, Anabas testudineus were tested. The females were given single injection of 7-12 mg PG/kg body weight and the males were given 4 mg PG/kg body weight. Fertilization and hatching rate varied from 67±4.55% to 66±3.0% and 59±4.88% to 57±6.21% for the doses of 10, 11 and 12 mg PG/kg of body weight, respectively. The hormone dose had significant (P<0.05) effect on fertilization and hatching. Six mini shallow cisterns (570 cm x 105 cm) were used to investigate the efficacy of zooplankton and Artemia nauplii as feed for spawn rearing. Three-day old spawns were stocked in six mini shallow cisterns at a stocking density of 100 individuals/L of water. Two treatments each with three replications were used to develop culture technique of the climbing perch. In case of treatment-1, the spawns were fed with Artemia nauplii three times daily, while in treatment-2, zooplankton were used as feed in the same manner as in treatment-1. After 14 days of rearing, mean final weight of the fry of treatments-1 and 2 were 95.55±6.71 and 57.69±5.40 mg, respectively. In treatment-1, spawn fed with Artemia nauplii showed significantly (P<0.05) higher mean weight than the spawn fed with zooplankton (treatment 2).

Ice storage characteristics of perch with special reference to its suitability for canning

Perch (Pagrus spinifer), one of the most abundantly available fishes of Gujarat coast, was subjected to a detailed study for assessing its storage life in ice and amenability of the iced fish for canning. Changes in the salt soluble nitrogenous material and myosin content of the iced fish showed good correlation with the changes in the organoleptic and physical qualities. The fish was found to have a storage life of 9 days in ice and samples stored up to 7 days were suitable for canning.

Integrated cage-cum-pond culture systems with high valued climbing perch (Anabas testudineus) in cages and low-valued carps in open ponds

An on-farm trial was conducted over 150 days to determine appropriate stocking ratio, growth and production of climbing perch (Anabas testudineus) in cages and carps in open water of ponds in eighteen farmers' ponds from Haluaghat Upazila at Mymensingh district of Bangladesh. One or two 1 m super(3) cage was suspended in each of 12 earthen ponds and other 6 ponds served as control without cages. Climbing perch of 2-3 g in size were stocked in cages while fingerlings of silver carp (Hypophthalmicthys molitrix), catla (Catla catla), rohu (Labeo rohita), mirgal (Cirhinus cirrhosus), rajputi (Puntius sarana) and common carp (Cyprinus carpio) were stocked at 1 fish/m super(2) with a species ratio of 5:4:4:4:2:1 in open water of all ponds to give cage to open-pond fish ratios of 1:1 (T sub(1:1)) and 2:1 (T sub(2:1)) and 0:1 (T sub(0:1)) as three treatments with six replicates each. Survival of climbing perch was higher in T sub(1:1) (61.67%) than that of T sub(2:1) (29.5%) and was significantly different (p>0.05) between the treatments. Stocking of small size climbing perch fry increased the mortality rate in cages. The net yields of Thai koi were 0.13±0.01 (t/ha) and 0.10±0.01 (t/ha) in treatments T sub(1:1) and T sub(2:1), respectively and both were significantly different (p>0.05). Survival of-open-pond carps was high, ranging from 50 to 91.67% with significantly lower in T sub(0:1) than that of T sub(1:1) and T sub(2:1) treatment. Net and gross yield of each carp species were significantly higher in the T sub(1:1) and T sub(2:1) treatment than that in T sub(0:1) treatment. Net revenues were positive but low in all treatments. Therefore, bigger size climbing perch with lower stocking ratio (T sub(1:1)) is suitable for integrated cage-pond culture of climbing perch and carps. However, more on-farm trials in different ecosystem with scientific interventions are necessary to develop the technology for further dissemination among the rural farmers.

Culture potentials of climbing perch, Anabas testudineus (Bloch) under different stocking densities at semi-intensive management

Anabas testudineus was cultured at different stocking density for the period of five months from May to September. Three stocking densities such as 50,000 (Treatment-1, T1), 56,250 (Treatment-2, T2) and 62,250/ha (Treatment-3, T3) were tested with three replications. After five months rearing, the mean weights of koi were 46.74±2.59, 40.44±2.98 and 37.27±3.01 in T-1, 2 and 3, respectively. The calculated production of native koi in T1 T2 and T3 were 1,916±314, 1,774.31 ±260 and 1,431 ±297 kg/ha, respectively which were significantly different (p<0.05) from each other.

The biology, ecology and impact of the Nile perch, Lates niloticus in lakes Victoria, Kyoga and Nabugabo and the future of the fisheries

Nile perch (Mputa), Lates niloticus was introduced into Lakes Victoria and Kyoga from lake Albert to increase fish production of these lakes by feeding on and converting the small sized haplochromines (Nkejje) which were abundant in these lakes into a larger table fish. It was, however, feared that Nile perch would prey on and deplete stocks of the native fishes and affect fish species diversity. Nile perch became well established and is currently among the three most important commercial species. It is presently the most important export fish commodity from Uganda. Considerable changes have taken place in fishery yield, and in life history characteristics of the Nile perch itself since the predator got established in Lakes Victoria and Kyoga.

The genetic structure and phylogenetics of pikeperch (Sander lucioperca) Anzali and Amirkolaye wetlands and perch (Perca fluviatilis) populations in Aras Dam and south-west of the Caspian Sea

The genetic structure of pikeperch (Sander lucioperca) and perch (Perca fluviatilis) populations was studied using microsatellite technique. A total of 207 specimens of adult pikeperch were collected from Aras dam (57 specimens), Anzali wetland (50 specimens), Talesh (50 specimens) and Chaboksar (50 specimens) coasts. Also a total of 158 specimens of adult perch were collected from Anzali (Abkenar (50 specimens)and Hendekhale(48 specimens)) and Amirkolaye(60 specimens) wetlands. About 2 g of each specimen's dorsal fin was removed, stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using ammonium-acetate method. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis. Polymerase Chain Reaction (PCR) was conducted on the target DNA using 15 pairs of microsatellite primers. PCR products were electrophoresed on poly acryl amide gels (6%) that were stained that were stained using silver nitrate. DNA bands were analyzed with BioCapt software. Allele count and frequency, genetic diversity, expected and observed heterozygosity , allele number and the effective allele number, genetic similarity and genetic distance, Fst, Rst, Hardy Weinberg Equilibrium based on X2 and Analysis of Molecular Variance (AMOVA) at 10% confidence level was calculated using the Gene Alex software. Dendogram for genetic distances and identities were calculated using TFPGA program for any level of hierarchy. The results for P. fluviatilis showed that from 15 pair of primers that were examined 6 polymorphic and 7 monomorphic loci were produced, while 2 loci didn't produce any DNA bands. Mean allele number was 4.1±1.1 and mean observed and expected heterozygosity was 0.56±0.12 and 0.58±0.14 respectively. It was also seen that specimens from all regions were not in Hardy Weinberg Equilibrium in some of loci (P<0.001). Highest Fst (0.095) with Nm=2.37 was observed between Hendekhale and Amirkolaye and the lowest Fst (0.004) with Nm=59.31 was observed between Abkenar and Hendekhale. According to AMOVA Significant difference (P<0.05) was observed between recorded Rst in the studied regions in Anzali and Amirkolaye lagoons. In another words there are two distinct populations of this species in Anzali and Amirkolaye lagoons. The highest genetic distance (0.181) and lowest genetic resemblance (0.834) were observed between specimens from Hendekhale and Amirkolaye and the lowest genetic distance (0.099) and highest genetic 176 resemblance (0.981) were observed between specimens from Abkenar and Hendekhale. Based on the genetic dendogram tree derived by applying UPGMA algorithm, specimens from Anzali and Amirkolaye wetlands have the same ancestor. On the other hand there is no noticeable genetic distance between the specimens of these two regions. Also the results for S. lucioperca showed that from 15 pair of primers that were examined 6 polymorphic and 7 monomorphic loci were produced, while 2 loci didn't produce any DNA bands. Mean allele number was 3.0±0.6 and mean observed and expected heterozygosity was 0.52±0.21 and 0.50±0.14 respectively. It was also seen that specimens from all regions were not in Hardy Weinberg Equilibrium in some of loci (P<0.001). Highest Fst (0.093) with Nm=2.43 was observed between Aras dam and Anzali wetland and the lowest Fst (0.022) with Nm=11.27 was observed between Talesh and Chaboksar coasts. Significant differences (P<0.05) were observed between recorded Rst in the studied regions exept for Talesh and Chaboksar Coasts. In another words there are three distinct populations of this species in Caspian sea, Anzali wetland and Aras dam. Highest genetic distance (0.110) and lowest genetic resemblance (0.896) were observed between specimens from Aras dam and Anzali wetland and the lowest genetic distance (0.034) and highest genetic resemblance (0.966) were observed between specimens from Talesh and Chaboksar coasts. Based on the genetic dendogram tree derived by applying UPGMA algorithm, specimens from Talesh and Chaboksar coasts have the lowest genetic distance. On the other hand the main population of this species belongs to Anzali wetland. Phylogenetic relationship of these two species was inferred using mitochondrial cytochrome b gene sequencing. For this purpose 2 specimens of P. fluviatilis from Anzali wetland, 2 specimens of S. lucioperca from Aras dam and 2 specimens of S. lucioperca from Anzali wetland were sequenced and submitted in Gene Bank. These sequences were aligned with Clustal W. The phylogenic relationships were assessed with Mega 4. The results of evolutionary history studies of these species using Neighbor-Joining and Maximum Parsimony methods showed that the evolutionary origin of pikeperch in Aras Dam and Anzali wetland is common. On the other hand these two species had common ancestor in about 4 million years ago. Also different sequences of any region specimens are supposed as different haplotypes. 177 As a conclusion the results of this study showed that microsatellite and mtDNA sequencing methods respectively are effective in genetic structure and phylogenic studies of P. fluviatilis and S. lucioperca.

Characterization of genetic biodiversity of Nile perch, Lates niloticus, Tilapiines, Haplochromine flock and Ningu (Labeo victorianus) in the Victoria Lake Basin: an overview

Genetic biodiversity is the vaflatlOn among individuals within and between units of interbreeding individuals (populations) of a species. It includes inheritable and transmittable differences that occur between individuals andlor popuhitions of a given species through reproductive interaction. There exists enormous variability among individuals andlor populations of a species for most living organisms, and most of this variation is inheritable. differences among individuals arise through mutation and via recombination of genes during meiosis. These ifferences are then transmitted to successive generations through sexual reproduction and maintained in the populations through processes such as natural selection and genetic drift. Unfortunately much of this variation is normally threatened and often in danger of extinction because most focus in conservation of natural resources is put at saving species or habitats than varieties or strains of a species

Fishing gear selectivity of Lates niloticus L. (Nile perch), Oreochromis niloticus L. (Nile tilapia) and Rastrineobola argentea Pellegrin (Mukene) in lakes Victoria, Kyoga and Nabugabo

Catch effort data on which fisheries management regulations are sometimes based are not available for most lakes in Uganda. However, failure to regulate fishing gears and methods has been a major cause of collapse of fisheries in the country. Fisheries have been damaged by destructive and non-selective fishing gears and methods such as trawling and beach seining, by use of gill nets of mesh size which crop immature fish and by introduction of mechanised fishing. Selectivity of the gears used to crop Lates niloticus 1. (Nile perch), Oreochromis niloticus 1. (Nile tilapia) and Rastrineobola argentea (Mukene) which are currently the most important commercial species in Uganda were examined in order to recommend the most suitable types, sizes and methods that should be used in exploiting these fisheries . Gill nets of less than 127 mm mainly cropped immature Nile ti1apia and Nile perch. To protect these fisheries, the minimum mesh size of gill nets should be set at 127 mm. Seine nets of 5 mm do catch high proportions of immature Mukene while those of 10 mm catch mainly mature Mukene. When operated inshore, both sizes catch immature Nile perch and Nile ti1apia as by-catch. To protect the Mukene fishery and avoid catching immature byecatch, a minimum mesh size of the Mukene net should have been 10 mm operated as Lampara type net offshore but since most fishennen have been using the 5 mm seine for over five years the minimum size should not be allowed to drop below 5 mm pending further thorough investigations. Beach seining, trawling and are destructive to fisheries and should be prohibited until data that may justify their use is available.