Human blood-mobilized hematopoietic precursors differentiate into osteoclasts in the absence of stromal cells.

Osteoclastogenesis is a complex process that is facilitated by bone marrow stromal cells (SCs). To determine if SCs are an absolute requirement for the differentiation of human hematopoietic precursors into fully mature, osteoclasts (OCs), CD34+ cells were mobilized into the peripheral circulation with granulocyte colony-stimulating factor, harvested by leukapheresis, and purified by magnetic-activated cell sorting. This procedure yields a population of CD34+ cells that does not contain SC precursors, as assessed by the lack of expression of the SC antigen Stro-1, and that differentiates only into hematopoietic cells. We found that CD34+, Stro-1- cells cultured with a combination of granulocyte/macrophage colony-stimulating factor, interleukin 1, and interleukin 3 generated cells that fulfill current criteria for the characterization of OCs, including multinucleation, presence of tartrate-resistant acid phosphatase, and expression of the calcitonin and vitronectin receptors and of pp60c-src tyrosine kinase. These OCs also expressed mRNA for the noninserted isoform of the calcitonin receptor and excavated characteristic resorption pits in devitalized bone slices. These data demonstrate that accessory SCs are not essential for human osteoclastogenesis and that granulocyte colony-stimulating factor treatment mobilizes OC precursors into the peripheral circulation.

Benedicti Sociaci ... Siluae & Opuscula sacra.

Benedicti Sociaci ... Opuscula sacra.

De electione et potestate praelatorum et potestate praelatorum, et aliorum officialium regularium. .. /

Mode of access: Internet.

Tractatus de duello, in quo quicquid ab vtroque iure, à Sacro Concilio Tridentino à Summis Pontificibus de Duello sancitum est, praecipuè vero Constitutio Clementis VIII Pont. Maximi accuratissimè explicatur ... /

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In passionem saluatoris secundum euangelium B. Ioannis /

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F. Francisci Panigarolae ecclesiae Hastensis episcopi Disceptationes Caluinicae ; a Ioanne Tonso mediolani patritio in latinum conuersae.

Edit16 CNCE47218.

Aphorismi alphabetico ordine dispositi pro vtroque foro, interno videlicet, [et] externo : ex doctorum consilijs collecti /

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De vita Hieronymi Aemiliani Congregationis Somaschae fundatoris : libri IV /

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De officio canonici e sacra scriptura Sanctorumque Patrum doctrina /

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Repertorio generale di giurisprudenza civile, commerciale, penale ed amministrativa contenente le decisioni delle magistrature del regno dall'unificazione legislativa (1866) a tutto il 1898,

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Orationes sex, sex presbyterorum Societatis Iesu ... : auctores, [et] argumenta sequens pagina indicat.

Edit16,

Tractatus de Saluiano interdicto /

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Annales sacri ab orbe condito ad ipsum Christi passione reparatum ... /

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On the temperature dependence of the rate coefficient of formation of C2+ from C + CH+

[EN] We carry out quasi-classical trajectory caculations for theC + CH+ → C2+ + H reaction on an ad hoc computed high-level ab initio potential energy surface. Thermal rate coefficients at the temperatures of relevance in cold interstellar clouds are derived and compared with the assumed, temperature-independent estimates publicly available in kinetic databases KIDA and UDfA. For a temperature of 10 K the database value overestimates by a factor of two the one obtained by us (thus improperly enhancing the destruction route of CH+ in astrochemical kinetic models) which is seen to double in the temperature range 5–300 K with a sharp increase in the first 50 K. The computed values are fitted via the popular Arrhenius–Kooij formula and best-fitting parameters α = 1:32 X 10-9 cm3s-1, β = 0:10 and γ = 2:19 K to be included in the online mentioned databases are provided. Further investigation shows that the temperature dependence of the thermal rate coefficient better conforms to the recently proposed so-called ‘deformed Arrhenius’ law by Aquilanti and Mundim.