Diário da Constituinte [gravação de vídeo] : [programa n. 205]

Grupo dos 32 (Trinta e dois) reúne-se para estudar os pontos críticos do substitutivo apresentado pelo Relator Bernardo Cabral, em busca de entendimento com o Grupo do Consenso em relação a temas como a reforma agrária, a anistia e sistema tributário. O Grupo dos 32 examinou também o capítulo sobre a administração pública. A Deputada Sandra Cavalcante (PFL-RJ) informa que a administração pública necessita de regras mais rígidas, de forma a acabar com o clientelismo dentro do Estado. Para o PMDB o substitutivo do relator, Deputado Bernardo Cabral (PMDB-AM), tem avanços importantes, mas há pontos que precisam refletir melhor as posições defendidas pelo partido. O Líder do PMDB, Deputado Mário Covas, considera que, tanto quanto possível, a Constituição será objeto de negociação, mas há certos temas que terão que ser decididos pelo voto no Plenário da Assembleia Nacional Constituinte (ANC). Um dos pontos que o PMDB quer mudar é a Reforma Agrária. Para José Eduardo Raduan, Presidente do Instituto Nacional de Colonização e Reforma Agrária (INCRA), é inviável a Reforma Agrária com imissão de posse após 90 dias da desapropriação. Projeto de Constituição não torna obrigatório o diploma de jornalismo para ser jornalista. O Deputado Carlos Alberto Oliveira (PDT-RJ) considera que é desastroso para o exercício de cidadania, porque a profissão de jornalista é tão especializada como tantas outras. A Deputada Cristina Tavares (PMDB-PE) declara que se trata de um dos mais graves retrocessos. Estudantes e professores de comunicação também são contra o fim da exigência de diploma. O professor de comunicação da UNB Wladimir de Carvalho declara que o caso parece ser resultado de um lobby da grande imprensa. Já o também professor de comunicação da UNB Murilo Ramos informa que o objetivo é abrir o mercado de mão-de-obra, para aumentar o lucro dos empresários. O Deputado Antônio Brito (PMDB-RS) afirma que a luta não é só dos jornalistas e sua situação profissional, mas que há preocupação com o país e com a qualidade da informação que os brasileiros receberão.

Poéticas da visualidade em João Cabral de Melo Neto e Joan Miró: a poesia como crítica de arte

A tese focaliza alguns dos principais poemas de João Cabral de Melo Neto desenvolvidos como crítica poética. O mote da investigação é a construção de uma lírica crítico-discursiva, uma produção artística que avalia a arte com os termos, os instrumentos da própria arte. Esta perspectiva analítica é deflagrada pelos primeiros românticos (Schlegel e Novalis) e expande-se em duas direções: tanto o poema escrito a partir de uma reflexão sobre a arte ou a natureza (crítico em sua gênese), quanto a crítica, transmutada em poesia. A abordagem comparativa de estâncias do poeta com obras picturais nelas referenciadas evidencia o constante diálogo do poeta com as poéticas da visualidade e com as concepções estéticas da modernidade, mormente com a poética de Joan Miró. Através da abordagem comparativa, materializam-se dois caminhos privilegiados de experimentação estética: o percurso do poético ao plástico, em João Cabral; o caminho inverso, na obra do pintor catalão do plástico ao poético. Para ambos os artistas, os processos de exploração das linguagens artísticas ocasionam, como consequência extrema, a dissolução do plástico e do poético, rumo ao inefável na pintura e na poesia. Para Miró, os signos plásticos são poéticos, porque guardam em si um universo de possibilidades significativas que transcendem a ordem da própria coisa em si. E o alcance das transformações que a sua poética empreende afetará a poesia de modo análogo: os signos poéticos mostram-se plenamente poéticos, ao transcenderem a forma e o sentido, no âmbito da plasticidade da palavra. Se a palavra pode se tornar matéria (passar da abstração para a concreção), a matéria pintada pode ser poesia. Integram a constelação teórica desta investigação os principais filósofos que discutem o caráter crítico da poesia, desde os filósofos do primeiro romantismo (Schlegel e Novalis), até aqueles cujo pensamento está relacionado à modernidade ou à pós-modernidade (Benjamin, Adorno, Lyotard, Blumenberg e Agamben)

PLOD3基因3'调控区的人类特异突变改变miR-124a对PLOD3的调控

microRNAs(miRNAs)是一类在转录后水平调控基因表达的不编码蛋白质的小RNA(长度20-24个碱基).其中,miR-124a是一个在哺乳动物中枢神经系统高度表达的miRNA,在神经前体细胞向神经元分化的过程中起着举足轻重的作用.由于miRNAs特异性地识别靶基因的3'端调控区(3'UTR)的靶序列,因此,在人类起源过程中基因3'UTR的单核苷酸序列变异有可能导致miRNA调控的改变.通过靶基因预测和3'UTR区在哺乳动物代表物种间的同源序列比较,我们发现miR-124a的靶基冈中有一个基因(PLOD3)3UTR的靶位点中存在人类特异突变位点.利用体外报告基因系统,发现PLOD3基因3'UTR靶位点中所含的一个人类特异的突变导致miR-124a对PLOD3的调控效率降低.研究表明,miRNAs靶基因3'UTR的序列变异具有功能效应,它有可能足人类中枢神经系统在起源和演化中发挥关键作用的重要遗传机制之一.

二(2,4,4-三甲基戊基)单硫代膦酸-上胺205-正庚烷乳状液膜萃取分离钪(Ⅲ)、铁(Ⅲ)和镥(Ⅲ)

本文研究了外水相ｐＨ值、流动载体浓度、表面活性剂浓度、内相解析酸浓度、水乳比、油内比等因素对二（２，４，４－三甲基戊基）单硫代膦酸（Ｃｙａｎｅｘ３０２）－上胺２０５－正庚烷乳状液 膜迁移Ｓｃ（Ⅲ）的影响。在一定条件下，Ｓｃ（Ⅲ）可以快速而完全地迁移，有可能实现Ｓｃ（Ⅲ）与Ｆｅ（Ⅲ）、Ｌｕ（Ⅲ）的分离。

异丙基膦酸单(1-己基-4-乙基)辛酯上胺205-正庚烷乳状液膜分离钪(Ⅲ)、铁(Ⅲ)、镥(Ⅲ)

本文研究了异丙基膦酸单（１－己基－４－乙基）辛酯（简称ＰＴ－２）上胺２０５－正庚烷乳状液膜分离钪（Ⅲ），铁（Ⅲ），镥（Ⅲ）及外相酸度、表面活性剂浓度、流动载体浓度和内相酸度对Ｓｃ（Ⅲ）、Ｆｅ（Ⅲ）、Ｌｕ（Ⅲ）迁移影响。实验结果表明，在一定的条件下Ｓｃ（Ⅲ）有可能实现与Ｆｅ（Ⅲ）、Ｌｕ（Ⅲ）的分离。

二(2,4,4-三甲基戊基)膦酸-上胺205-正庚烷乳状液膜分离钪(Ⅲ)、铁(Ⅲ)和镥(Ⅲ)

本文研究了二（2，4，4-三甲基戊基）膦酸（Cyanex272）-上胺205-正庚烷乳状液膜分离Sc（Ⅲ）、Fe（Ⅲ）和Lu（Ⅲ）。考察了外相酸度、表面活性剂浓度、流动载体浓度及内相盐酸浓度对Sc（Ⅲ）迁移的影响。并探讨了该液膜体系分离Sc（Ⅲ）、Fe（Ⅲ）和Lu（Ⅲ）的可能性，结果表明，该体系对于稀土矿中Sc（Ⅲ）的分离与分析有良好的应用前景。

A miR-34a-Numb Feedforward Loop Triggered by Inflammation Regulates Asymmetric Stem Cell Division in Intestine and Colon Cancer.

Emerging evidence suggests that microRNAs can initiate asymmetric division, but whether microRNA and protein cell fate determinants coordinate with each other remains unclear. Here, we show that miR-34a directly suppresses Numb in early-stage colon cancer stem cells (CCSCs), forming an incoherent feedforward loop (IFFL) targeting Notch to separate stem and non-stem cell fates robustly. Perturbation of the IFFL leads to a new intermediate cell population with plastic and ambiguous identity. Lgr5+ mouse intestinal/colon stem cells (ISCs) predominantly undergo symmetric division but turn on asymmetric division to curb the number of ISCs when proinflammatory response causes excessive proliferation. Deletion of miR-34a inhibits asymmetric division and exacerbates Lgr5+ ISC proliferation under such stress. Collectively, our data indicate that microRNA and protein cell fate determinants coordinate to enhance robustness of cell fate decision, and they provide a safeguard mechanism against stem cell proliferation induced by inflammation or oncogenic mutation.

MiR-215 Is Induced Post-transcriptionally via HIF-Drosha Complex and Mediates Glioma-Initiating Cell Adaptation to Hypoxia by Targeting KDM1B.

The hypoxic tumor microenvironment serves as a niche for maintaining the glioma-initiating cells (GICs) that are critical for glioblastoma (GBM) occurrence and recurrence. Here, we report that hypoxia-induced miR-215 is vital for reprograming GICs to fit the hypoxic microenvironment via suppressing the expression of an epigenetic regulator KDM1B and modulating activities of multiple pathways. Interestingly, biogenesis of miR-215 and several miRNAs is accelerated post-transcriptionally by hypoxia-inducible factors (HIFs) through HIF-Drosha interaction. Moreover, miR-215 expression correlates inversely with KDM1B while correlating positively with HIF1α and GBM progression in patients. These findings reveal a direct role of HIF in regulating miRNA biogenesis and consequently activating the miR-215-KDM1B-mediated signaling required for GIC adaptation to hypoxia.

De la cooperativa agroalimentaria a la "learning netchain". Hacia un planteamiento teorico interorganizativo e interpersonal

Se propone un planteamiento teórico/conceptual para determinar si las relaciones interorganizativas e interpersonales de la netchain de las cooperativas agroalimentarias evolucionan hacia una learning netchain. Las propuestas del trabajo muestran que el mayor grado de asociacionismo y la mayor cooperación/colaboración vertical a lo largo de la cadena están positivamente relacionados con la posición horizontal de la empresa focal más cercana del consumidor final. Esto requiere una planificación y una resolución de problemas de manera conjunta, lo que está positivamente relacionado con el mayor flujo y diversidad de la información/conocimiento obtenido y diseminado a lo largo de la netchain. Al mismo tiempo se necesita desarrollar un contexto social en el que fluya la información/conocimiento y las nuevas ideas de manera informal y esto se logra con redes personales y, principalmente, profesionales y con redes internas y, principalmente, externas. Todo esto permitirá una mayor satisfacción de los socios de la cooperativa agroalimentaria y de sus distribuidores y una mayor intensidad en I+D, convirtiéndose la netchain de la cooperativa agroalimentaria, así, en una learning netchain.

miR-7 and miR-214 are specifically expressed during neuroblastoma differentiation, cortical development and embryonic stem cells differentiation, and control neurite outgrowth in vitro

The mammalian nervous system exerts essential control on many physiological processes in the organism and is itself controlled extensively by a variety of genetic regulatory mechanisms. microRNA (miR), an abundant class of small non-coding RNA, are emerging as important post-transcriptional regulators of gene expression in the brain. Increasing evidence indicates that miR regulate both the development and function of the nervous system. Moreover, deficiency in miR function has also been implicated in a number of neurological disorders. Expression profile analysis of miR is necessary to understand their complex role in the regulation of gene expression during the development and differentiation of cells. Here we present a comparative study of miR expression profiles in neuroblastoma, in cortical development, and in neuronal differentiation of embryonic stem (ES) cells. By microarray profiling in combination with real time PCR we show that miR-7 and miR-214 are modulated in neuronal differentiation (as compared to miR-1, -16 and -133a), and control neurite outgrowth in vitro. These findings provide an important step toward further elucidation of miR function and miR-related gene regulatory networks in the mammalian central nervous system. (C) 2010 Elsevier Inc. All rights reserved.

Deep sequencing reveals predominant expression of miR-21 amongst the small non-coding RNAs in retinal microvascular endothelial cells

The retinal vascular endothelium is essential for angiogenesis and is involved in maintaining barrier selectivity and vascular tone. The aim of this study was to identify and quantify microRNAs and other small regulatory non-coding RNAs (ncRNAs) which may regulate these crucial functions. Primary bovine retinal microvascular endothelial cells (RMECs) provide a well-characterized in vitro system for studying angiogenesis. RNA extracted from RMECs was used to prepare a small RNA library for deep sequencing (Illumina Genome Analyzer). A total of 6.8 million reads were mapped to 250 known microRNAs in miRBase (release 16). In many cases, the most frequent isomiR differed from the sequence reported in miRBase. In addition, five novel microRNAs, 13 novel bovine orthologs of known human microRNAs and multiple new members of the miR-2284/2285 family were detected. Several similar to 30 nucleotide sno-miRNAs were identified, with the most highly expressed being derived from snoRNA U78. Highly expressed microRNAs previously associated with endothelial cells included miR-126 and miR-378, but the most highly expressed was miR-21, comprising more than one-third of all mapped reads. Inhibition of miR-21 with an LNA inhibitor significantly reduced proliferation, migration, and tube-forming capacity of RMECs. The independence from prior sequence knowledge provided by deep sequencing facilitates analysis of novel microRNAs and other small RNAs. This approach also enables quantitative evaluation of microRNA expression, which has highlighted the predominance of a small number of microRNAs in RMECs. Knockdown of miR-21 suggests a role for this microRNA in regulation of angiogenesis in the retinal microvasculature. J. Cell. Biochem. 113: 20982111, 2012. (C) 2012 Wiley Periodicals, Inc.

A novel dual-fluorescence strategy for functionally validating microRNA targets in 3-prime untranslated regions: regulation of the inward rectifier potassium channel Kir2.1 by miR-212.

Gene targeting by microRNAs is important in health and disease. We developed a functional assay for identifying microRNA targets and applied it to the K+ channel Kir2.1 (KCNJ2) which is dysregulated in cardiac and vascular disorders. The 3'UTR was inserted downstream of the mCherry red fluorescent protein coding sequence in a mammalian expression plasmid. MicroRNA sequences were inserted into the pSM30 expression vector which provides enhanced green fluorescent protein as an indicator of microRNA expression. HEK293 cells were co-transfected with the mCherry-3'UTR plasmid and a pSM30-based plasmid with a microRNA insert. The principle of the assay is that functional targeting of the 3'UTR by the microRNA results in a decrease in the red/green fluorescence intensity ratio as determined by automated image analysis. The method was validated with miR-1, a known downregulator of Kir2.1 expression, and was used to investigate targeting of the Kir2.1 3'UTR by miR-212. Red/green ratio was lower in miR-212-expressing cells compared to non-targeting controls, an effect that was attenuated by mutating the predicted target site. MiR-212 also reduced inward rectifier current and Kir2.1 protein in HeLa cells. This novel assay has several advantages over traditional luciferase-based assays including larger sample size, amenability to time course studies and adaptability to high-throughput screening.

Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses

Incorporation of Ags by dendritic cells (DCs) increases when Ags are targeted to endocytic receptors by mAbs. We have previously demonstrated in the mouse that mAbs against C-type lectins administered intradermally are taken up by epidermal Langerhans cells (LCs), dermal Langerin(neg) DCs, and dermal Langerin(+) DCs in situ. However, the relative contribution of these skin DC subsets to the induction of immune responses after Ag targeting has not been addressed in vivo. We show in this study that murine epidermal LCs and dermal DCs transport intradermally injected mAbs against the lectin receptor DEC-205/CD205 in vivo. Skin DCs targeted in situ with mAbs migrated through lymphatic vessels in steady state and inflammation. In the skin-draining lymph nodes, targeting mAbs were found in resident CD8a(+) DCs and in migrating skin DCs. More than 70% of targeted DCs expressed Langerin, including dermal Langerin(+) DCs and LCs. Numbers of targeted skin DCs in the nodes increased 2-3-fold when skin was topically inflamed by the TLR7 agonist imiquimod. Complete removal of the site where OVA-coupled anti-DEC-205 had been injected decreased endogenous cytotoxic responses against OVA peptide-loaded target cells by 40-50%. Surprisingly, selective ablation of all Langerin(+) skin DCs in Langerin-DTR knock-in mice did not affect such responses independently of the adjuvant chosen. Thus, in cutaneous immunization strategies where Ag is targeted to DCs, Langerin(+) skin DCs play a major role in transport of anti-DEC-205 mAb, although Langerin(neg) dermal DCs and CD8a(+) DCs are sufficient to subsequent CD8(+) T cell responses.