The binding and catalytic properties of lysozyme

The binding and catalytic properties of hen's egg white lysozyme have been studied by a variety of techniques. These studies show that the enzyme has three contiguous binding subsites, A, B, and C. The application of nuclear magnetic resonance (NMR) spectroscopy to probe the binding environment of several saccharides to lysozyme has demonstrated that the reducing end sugar rings of chitotriose, chitobiose and the β-form of N-acetylglucosamine all bind in subsite C. The central sugar ring of chitotriose and the sugar ring at the nonreducing end of chitobiose were found to bind in subsite B, while the nonreducing end sugar residue of chitotriose occupied subsite A. The dynamics of the binding process has also been investigated by NMR. The formation rate constant of chitobiose--and chitotriose-enzyme complexes were found to be about 4 X 10^-6 M^-1 sec^-1 with small activation energies.

The stereochemical path of the lysozyme catalyzed hydrolysis of glycosidic bonds has been shown to proceed with at least 99.7% retention of configuration at C-1 of the sugar. The lysozyme catalyzed hydrolysis of glucosidic bonds has been shown to be largely carbonium ion in character by virtue of the α-deuterium kinetic isotope effect (k_H/k_D = 1.11) observed for the reaction. It is probable that the mechanism of action of the enzyme involves a carbonium ion intermediate which is stereospecifically quenched by solvent. However, acetamido group participation cannot be ruled out for natural substrates.

Utilisation of trash fish. Pt. 2. Studies on preparation of fish soup mix

The paper deals with the investigations carried out on the preparation of fish soup mix using partially deodorized trash fish meat. The product contained about 25% protein and had a storage life of 4 months at ambient temperature (28-31°C).

Anvers, plongeon de [Harold] Clarke [plongeon haut simple, Jeux olympiques] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 60696

The unkown murderer

UANL

Women's Physical Education. Ground breaking for new swimming pool unit

Black & Black, architects. Dedicated April 17, 1954 and named after Dr. Margaret Bell, chairman of the Program of Physical Education for Women. Left to right: President Harlan Hatcher; Dr. Margaret Bell, director of Women's Physical Ed. program; and H.O. (Fritz) Crisler

The dyeing of textile fabrics.

Includes index.

The history of the province of Moray. Comprising the counties of Elgin and Nairn, the greater part of the county of Inverness and a portion of the county of Banff,--all called the province of Moray before there was a division into counties.

Mode of access: Internet.

Jewish wit.

Mode of access: Internet.

Agustín de Rojasʾ El natural desdichado /

Originally presented as the editor's thesis, Cornell University, 1929.