Genetic variation and evolutionary divergence within and among populations, species, and genera of the Cambarinae

Seven crayfish species from three genera of the subfamily Cambarinae were electrophoretically examined for genetic variation at a total of twenty-six loci. Polymorphism was detected primarily at three loci: Ao-2, Lap, and Pgi. The average heterozygosities over-all loci for each species were found to be very low when compared to most other invertebrate species that have been examined electrophoretically. With the exception of Cambarus bartoni, the interpopulation genetic identities are high within any given species. The average interspecific identities are somewhat lower and the average intergeneric identities are lower still. Populations, species and genera conform to the expected taxonomic progression. The two samples of ~ bartoni show high genetic similarity at only 50 percent of the loci compared. Locus by locus identity comparisons among species yield U-shaped distributions of genetic identities. Construction of a phylogenetic dendrogram using species mean genetic distances values shows that species grouping is in agreement with morphological taxonomy with the exception of the high similarity between Orconectespropinquus and Procambarus pictus. This high similarity suggests the possibility of a regulatory change between the two species. It appears that the low heterozygosities, high interpopulation genetic identities, and taxonomic mispositioning can all be explained on the basis of low mutation rates.

Feasibility of the Reintroduction of Wolves in Maine: a GIS Study

The eastern timber wolf (Canis lupus lycaon) once inhabited Maine, as well as the rest of the eastern United States and southern Canada. As a result of human land use and widespread extermination campaigns, wolf numbers dramatically decreased, and by the early twentieth century, no wolves remained in Maine. As large carnivorous and territorial mammals, wolves require contiguous undeveloped areas with abundant prey. This project is a feasibility study that identifies the areas in Maine that are suitable for the reintroduction of wolves. We used GIS modeling to identify contiguous forested areas over 1,000 km2, calculate road and population density, and map the presence or absence of prey throughout the state. These variables were combined in a habitat suitability model to determine the location and amount of suitable wolf habitat in Maine. The northwestern part of the state appears most suitable for wolf reintroduction as it is relatively undeveloped with low road and population densities. There is also a smaller isolated area in Washington County that might be suitable, but further investigation is required.

Cytogenetical aspects of testicular cells in economically important species of coreidae family (Heteroptera)

Some cytogenetical aspects of spermatozoa formation were studied in 9 Coreidae Brazilian species: Anasa bellator, Athaumastus haematicus, Chariesterus armatus, Dallacoris obscura, Dallacoris pictus, Leptoglossus gonagra, Leptoglossus zonatus, Sphictyrtus fasciatus, and Zicca annulata. Similarly to the other species described to date, all the species studied herein showed cystic spermatogenesis, a reddish membrane covering the testes, a X0 sex determining system, a pair of m-chromosomes, intersticial chiasmata in most autosomes, and autosomes dividing reductionally at first meiotic division and equationally in the second 1 while sex chromosomes, divide equationally and reductionally at first and second meiotic division, respectively. In addition, it was observed that the sex chromosome is heteropycnotic at prophase and that heteropycnotic chromosomal material is found in the nuclei at spermiogenesis. In the species studied, the diploid chromosome number ranged from 19 to 25. It was 19 in S. fasciatus (16A+2m+X0); 21 in A. bellator, A. haematicus, D. obscura, D. pictus, L. gonagra, and L. zonatus (18A+2m+X0); 23 in Z. annulata (20A+2m+X0); and 25 in C. armatus (22A+2m+X0). © 2007 The Japan Mendel Society.

Foregut morphology of Pseudothelphusidae and Trichodactylidae (Decapoda: Brachyura) from northeastern Pará, Brazil

The gross morphology of foregut was investigated and described in detail for four freshwater crab species: a pseudothelphusid, Fredius reflexifrons (Ortmann, 1897) and three trichodactylids - Sylviocarcinus pictus (H. Milne-Edwards, 1853), Valdivia serrata White, 1847, and Dilocarcinus septemdentatus (Herbst, 1783). In general, the gastric mills of freshwater crabs showed a similar degree of complexity in relation to other marine species of Anomura and Brachyura previously described in the literature. However, significant morphological differences were observed among the analyzed species, as in ossicle numbers, features and connection types. A total of 38 ossicles were described for S. pictus and V. serrata and 37 ossicles for D. septemdentatus and F. reflexifrons, respectively. The latter species can be distinguished from the others by the presence of a wide cardiac sac resembling two sacs. Most of the ossicles were connected through a rigid connection, and few ossicles were fused. The degree of calcification varied little among the studied species. The differences found among the species suggest that the morphological aspects of the foregut may be useful in the identification of similar species.

Revisão do gênero Antiteuchus Dallas (Heteroptera, Pentatomidae, Discocephalinae)

Revisão do gênero Antiteuchus Dallas (Heteroptera, Pentatomidae, Discocephalinae). O gênero Antiteuchus Dallas, 1851 com 46 espécies descritas é revisado; as espécies são redescritas com base na morfologia externa da genitália do macho e em algumas características da morfologia geral do corpo. Neodine Kirkaldy, 1909 é considerado sinônimo junior de Antiteuchus. As espécies foram subdivididas em seis grupos aqui propostos: A. amplus, A. supinatus, A. peruensis, A. tesselatus, A. marmoratus e A. mixtus. Oito novas espécies são descritas: A. amapensis sp. nov., A. beckerae sp. nov., A. doesburgi sp. nov., A. exiguus sp. nov., A. ledeburi sp. nov., A. marini sp. nov., A. melanicus sp. nov. and A. similis sp. nov. Nove espécies e uma subespécie são sinonimizadas. Antiteuchus varians Ruckes, 1964 é considerada sinônimo júnior de A. pallescens Stål, 1868; A. englemani Rolston, 1993 de A. amplus (Walker, 1867); A. tripterus limbativentris Ruckes, 1964 e A. minor Engleman, 1983 de A. tripterus (Fabricius, 1787); A. fuscus (Ruckes, 1959), A. piceus (Palisot de Beauvois, 1805), A. subgibbus Engleman, 1983, A. subimpunctatus Ruckes, 1964, A. unicolor (Westwood, 1837) e A. variolosus (Westwood, 1837) de A. mixtus (Fabricius, 1787). Antiteuchus tatei (Ruckes, 1958) é considerada species inquirenda. Empicoris marmoreus Spinola, 1837 é colocada em incertae sedis e tratada como species inquirenda. O macho de A. pictus, até agora desconhecido, é descrito. Chaves de identificação para os machos das espécies de Antiteuchus e para os grupos de espécies são apresentadas.

Biodiversity of decapod crustaceans in the estuarine floodplain around the city of Belém (Pará) in Brazilian Amazonia

ABSTRACT: The present study focused on the decapod fauna of the fluvial-estuarine environment of the Guajará Bay, in the Brazilian state of Pará, where specimens were collected monthly from six sites, from May 2006 to April 2007. A total of 6,793 specimens were captured, belonging to 11 species of crab and shrimp: eight palaemonids - Macrobrachium amazonicum (Heller, 1862), Macrobrachium surinamicum Holthuis, 1948, Macrobrachium carcinus (Linnaeus, 1758), Macrobrachium rosenbergii (De Man, 1879), Macrobrachium spp. 1-4 -, one portunid - Callinectes bocourti A. Milne-Edwards, 1879 -, and two trichodactylids - Sylviocarcinus devileii H. Milne-Edwards, 1853 and Sylviocarcinus pictus (Milne-Edwards, 1853). While no significant differences were found in the ecological indices of diversity with respect to season, site, or trap size, a tendency for increased abundance and species richness was found during the dry season (August-November), in particular at some sites, apparently reflecting the influence of the estuary's saline wedge.

Characterization of sea urchin yolk glycoproteins

To study the fate of the yolk glycoproteins found in eggs and embryos of the sea urchin, S. purpuratus, a polyclonal antibody to a 90-kDa polymannose glycoprotein was prepared. lmmunoblot analysis of total proteins over the course of development showed that this antibody recognized a family of glycoproteins. Concomitant with the disappearance of the major 160-kDa egg yolk glycoprotein during embryogenesis, glycoproteins with a lower molecular mass appeared. These glycoproteins (115, 108, 90, 83, and 68 kDa) were purified and peptide mapping revealed that they were cleavage products derived from the major yolk glycoprotein. The antibody identified a homologous set of yolk glycoproteins with similar molecular masses in the embryos of three other species in the class Echinoidea: L. pictus, A. punctulata, and D. excentricus. However, eggs from other echinoderm classes and from chicken, frog, fruit fly, and nematode did not contain any cross-reactive molecules. Cross-reactivity within the class Echinoidea was not due to a common carbohydrate epitope, because the antibody recognized the glycoproteins even after the N-linked, polymannose carbohydrate side chains were enzymatically removed. The major yolk glycoprotein (160-170 kDa) from each of the three sea urchin species was purified and analyzed, revealing striking similarities in pI and in amino acid and monosaccharide composition. Peptide mapping showed that the 160-kDa glycoprotein from the four echinoids are structurally homologous. The major yolk glycoprotein appeared to be proteolyzed by a thiol protease, which could be activated in yolk particles prepared from unfertilized eggs by low pH. Immunolocalization by electron microscopy in S. purpuratus showed that the yolk glycoproteins remained within the yolk platelet throughout embryonic development, and that externalization of the glycoproteins was not detectable. The yolk glycoprotein precursor began to be synthesized in premetamorphosis larvae, and continued in adult males and females. Both the yolk glycoproteins and the yolk platelets disappeared during larval development. This disappearance has special significance because there were no yolk proteins in the direct developing sea urchin, H. erthryogramma, which bypasses larval development and metamorphoses directly into an adult. ^

Gene expression in sea urchin development: Study of {\it LpS1\/} gene regulation and cloning and characterization of the {\it SpKrox}-{\it 1\/} gene

Cell differentiation are associated with activation of cell lineage-specific genes. The $LpS{\it 1}\beta$ gene of Lytechinus pictus is activated at the late cleavage stage. $LpS{\it 1}\beta$ transcripts accumulate exclusively in aboral ectoderm lineages. Previous studies demonstrated two G-string DNA-elements, proximal and distal G-strings, which bind to an ectoderm-enriched nuclear factor. In order to define the cis-elements which control positive expression of the $LpS{\it 1}\beta$ gene, the regulatory region from $-$108 to +17 bp of the $LpS{\it 1}\beta$ gene promoter was characterized. The ectoderm G-string factor binds to a G/C-rich region larger than the G-string itself and the binding of the G-string factor requires sequences immediately downstream from the G-string. These downstream sequences are essential for full promoter activity. In addition, only 108 bp of $LpS{\it 1}\beta\ 5\sp\prime$ flanking DNA drives $LpS{\it 1}\beta$ gene expression in aboral ectoderm/mesenchyme cells. Therefore, for positive control of $LpS{\it 1}\beta$ gene expression, two regions of 5$\sp\prime$ flanking DNA are required: region I from base pairs $-$762 to $-$511, and region II, which includes the G/C-rich element, from base pairs $-$108 to $-$61. A mesenchyme cell repressor element is located within region I.^ DNA-binding proteins play key roles in determination of cell differentiation. The zinc finger domain is a DNA-binding domain present in many transcription factors. Based on homologies in zinc fingers, a zinc finger-encoding gene, SpKrox-1, was cloned from S. purpuratus. The putative SpKrox-1 protein has all structural characteristics of a transcription factor: four zinc fingers for DNA binding; acidic domain for transactivation; basic domain for nuclear targeting; and leucine zipper for dimerization. SpKrox-1 RNA transcripts showed a transient expression pattern which correlates largely with early embryonic development. The spatial expression of SpKrox-1 mRNA was distributed throughout the gastrula and larva ectodermal wall. However, SpKrox-1 was not expressed in pigment cells. The SpKrox-1 gene is thus a marker of a subset of SMCs or ectoderm cells. The structural features, and the transient temporal and restricted spatial expression patterns suggest that SpKrox-1 plays a role in a specific developmental event. ^

Benthos fauna in the North Sea from 2 surveys in 1986 and 1987

During two surveys in the North Sea, in summer 1986 and in winter 1987, larger epibenthos was collected with a 2 m beam trawl. The distributions of the species were checked for average linkage by means of the JACCARD-index cluster analysis. In summer two main clusters can be recognized. These are situated to the north and to the south of the Dogger Bank. In winter two main clusters may be recognized as well, but these clusters divide the North Sea into a western and an eastern part. We conclude, that these differences of epibenthos characteristics are correlated with seasonal changes in water body distributions.

Vertical distribution of pelagic polychaetes in Kuril-Kamchatka Trench plankton in August 1966

Vertical distribution patterns of four mass species of pelagic polychaetes (with separate consideration of the adult, juvenile and larval stages) in plankton of the Kuril-Kamchatka region from the surface to depth of 7000 m are analyzed. This material, the author's personal data on the Norwegian and Barents Seas, and literature data on other seas are used to distinguish some general patterns in vertical distribution and reproductive ecology of pelagic polychaetes in the World Ocean as a whole. Mass pelagic polychaetes are also compared to other bathypelagic animals with respect to these ecological features and an estimate is given of their abundance in ocean plankton.