Avaliação do método de fotooxidação do DiI por diodo emissor de luz (led): aspectos morfológicos de células horizontais da retina humana

A análise da morfologia celular é um aspecto crucial da neurobiologia, pois a relação entre forma e função pode definir os processos fisiológicos na saúde e na doença. Um dos principais métodos para avaliar a morfologia celular é por meio da fotooxidação de marcadores fluorescentes intracelulares, dentre os quais se tem o percloreto de 1,1’-dioctadecil-3,3,3’,3’-tetrametil-indocarbocianina (DiI), uma carbocianina lipofílica que pode marcar células vivas ou fixadas. O DiI foi escolhido para este trabalho devido, dentre outros fatores, à sua importante utilização para estudos de morfologia celular. Como modelo para avaliar a qualidade da fotooxidação do aparelho construído para essa finalidade, elegeu-se as células horizontais da retina humana com o intuito de prosseguir com estudo morfométrico posterior dessas células, tendo em vista a escassez de trabalhos com essa abordagem em retina humana. Assim, este estudo teve como objetivo avaliar a qualidade do método de fotooxidação do DiI através de LED e utilizando como modelo células horizontais da retina humana. O material foi obtido do Banco de Olhos do Hospital Ophir Loyola e, em sequência dissecado, marcado com cristais de DiI e fotooxidado com o aparelho de LED. As imagens que resultaram do novo método de iluminação para fotooxidação de traçador fluorescente apresentou elevada qualidade de detalhes da morfologia neuronal, similares aos resultados obtidos em reações de fotoconversão convencional com microscópio, o que permite concluir que o aparelho mantém a eficiência da fotooxidação por revelar detalhes finos da morfologia celular, inclusive com as vantagens de processar áreas maiores de tecido e considerável redução de custo por dispensar o emprego de microscópio para o processo.

Proposta de metodologia para criação e análise de cenários na continuidade do Programa Reluz, utilizando tecnologia LED em sistemas de iluminação pública

Pós-graduação em Engenharia Mecânica - FEG

Investigations on PVP/Y3BO6:Eu3+, a red Luminescent composite for lighting devices based on near UV-LEDs

This work deals with a red phosphor. Y3BO6:Eu3+, and its corresponding poly(N-vinylpyrrolidone) (PVP)/Y3BO6:Eu3+ luminescent composite film suitable for applications in the next generation of Hg-free lamps based on near ultraviolet (UV) light emitting diodes (LEDs). Well crystallized samples of Y3BO6 powders with the Eu3+ content up to 20 mol% were prepared by the Pechini method. After structural, morphological and optical characterization, the best doping rate of Eu3+ in the matrix was determined to be 15 mol%. This optimal powder, which is highly friable, was easily ground into fine particles and homogeneously dispersed into a PVP polymer solution to give rise to a polymer phosphor composite. Structural and optical features of the composite film have been studied and compared to those of a pristine PVP film and Y3BO6:Eu3+ powder. All the characterization (XRD, SAXS, luminescence...) proved that the red phosphor particles are well incorporated into the polymer composite film which exhibited the characteristic red emission of Eu3+ under UV light excitation. Furthermore, photostability of the polymer/phosphor composite film under UV-LED irradiation was evaluated from exposure to accelerated artificial photoageing at wavelengths above 300 nm.

Iluminação de exteriores utilizando tecnologia LED: estudo de caso

This Graduate Work aims to exhibit the analyze of the applicability of LED technology in a real outdoor lighting situation, and this project’s illumination solution is compared with the initial proposal, the sodium vapor lamps. What is studied is an outdoor area, like the roads and parking lots of a plant. A light planning software is used for this comparison, and the results are displayed instantly, allowing rapid analysis of the project through a simulation. The medium illuminance is the same in both solutions for this case study and minimum rates set as standard were observed then the analysis are appropriate in this project. Various aspects are considered in order to obtain a comprehensive analysis of this project, such as economic, environmental and quality of lighting. LED technology in outdoor lighting is presented in its infancy, but promising. Solid state lamps show great advantages when compared with other technologies, such as discharge lamps, however there are some disadvantages tied to new products that are expected to be overcome with technological development and increased production

Plano de iluminação externa da UNESP Campus Itapeva com base em LED e energia solar

This work presents the development of the External Lighting Plan UNESP in the Itapeva Campus based LED and Solar Energy. Firstly it was made a collection of data from measurements of the local transit through the Google Earth 6 software and divide the local in sectors, and then perform an analysis of characteristics and age of the site. With these data it was possible to determine the average luminance the place, in30 lux. After these procedures was possible to determine the type of sets that would perform the role of bridge lighting the kit chosen was the 20/20 Sun LED manufacturer, since it contains the luminaire with the solar panel to the pole. Therefore we determined the number of poles for each sector, whichwas72in total and. After the determination of the location of each point lighting

Avaliação in vitro do tempo de fotopolimerização com led de alta potência de bráquetes ortodônticos e remoção de adesivo remanescente sob luz ultravioleta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Newer optics efficiently mix, dim, and color-tune LED light

Low-cost, plastic-injected optics mix light from different color LED dies without a significant decrease in average brightness, simplifying luminaire design both optically and electronically. In solid-state lighting, high-flux and high-color rendering index (CRI) light engines may be achieved by arraying and mixing the light from different color dies or phosphors, or a combination of the two, in the LED package. However, these nonhomogeneous sources, when combined with luminaire optics, tend to produce patterns with undesirable artifacts such as spatial and angular nonuniformities and color separation.

LED bulb lamps technical specification and testing procedure for solar home systems.

With the consolidation of the new solid state lighting LEOs devices, te5t1n9 the compliance 01 lamps based on this technology lor Solar Home Systems (SHS) have been analyzed. The definition of the laboratory procedures to be used with final products 15 a necessary step in arder to be able to assure the quality of the lamps prior to be installed [1]. As well as with CFL technology. particular attention has been given to simplicity and technical affordability in arder to facilitate the implementation of the test with basie and simple laboratory too15 even on the same SHS electrification program locations. The block of test procedures has been applied to a set of 14 low-cost lamps. They apply to lamp resistance, reliability and performance under normal, extreme and abnormal operating conditions as a simple but complete quality meter tool 01 any LEO bulb.

Lighting technology

From the economic point of view, additional lighting at night causes enormous costs and is responsible for using up a massive percentage of the worlds energy resources. During recent years, development trends in the field of LED technology have paved the way for novel concepts impacting on the subject oflight. In this way, the abovementioned issues can be greatly reduced resulting in a better balance between the environment and human needs.

Merit function for the evaluation of color uniformity in the far field of LED spot lights

The scope of the present paper is the derivation of a merit function which predicts the visual perception of LED spot lights. The color uniformity level Usl is described by a linear regression function of the spatial color distribution in the far field. Hereby, the function is derived from four basic functions. They describe the color uniformity of spot lights through different features. The result is a reliable prediction for the perceived color uniformity in spot lights. A human factor experiment was performed to evaluate the visual preferences for colors and patterns. A perceived rank order was derived from the subjects’ answers and compared with the four basic functions. The correlation between the perceived rank order and the basic functions was calculated resulting in the definition of the merit function Usl. The application of this function is shown by a comparison of visual evaluations and measurements of LED retrofit spot lamps. The results enable a prediction of color uniformity levels of simulations and measurements concerning the visual perception. The function provides a possibility to evaluate the far field of spot lights without individual subjective judgment. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Optimization of optical systems for LED spot lights concerning the color uniformity

Spotlighting is one illumination field where the application of light emitting diodes (LED) creates many advantages. Commonly, the system for spot lights consists of a LED light engine and collimating secondary optics. Through angular or spatial separated emitted light from the source and imaging optical elements, a non uniform far field appears with colored rings, dots or patterns. Many feasible combinations result in very different spatial color distributions. Several combinations of three multi-chip light sources and secondary optical elements like reflectors and TIR lenses with additional facets or scattering elements were analyzed mainly regarding the color uniformity. They are assessed by the merit function Usl which was derived from human factor experiments and describes the color uniformity based on the visual perception of humans. Furthermore, the optical systems are compared concerning efficiency, peak candela and aspect ratio. Both types of optics differ in the relation between the color uniformity level and other properties. A plain reflector with a slightly color mixing light source performs adequate. The results for the TIR lenses indicate that they need additional elements for good color mixing or blended light source. The most convenient system depends on the requirements of the application.

Implementación y análisis de luminarias LED

En este proyecto se abordan el diseño y análisis, con sus diferentes etapas, de varias configuraciones de prototipos para luminarias LED enfocadas al ámbito domestico e industrial. Se realizarán montajes sobre diferentes materiales para evaluar los resultados y decidir que soporte es más adecuado para cada circunstancia. Inicialmente expondremos el estado actual de esta tecnología y caracterizaremos, de forma preliminar, los dispositivos que vamos a utilizar para elaborar los prototipos que posteriormente analizaremos. Seguidamente detallaremos el proceso de construcción de cada prototipo, indicando especialmente las diferencias y similitudes entre ellos que, en siguientes secciones, serán analizadas. Trabajaremos con dos rangos de potencia para las luminarias. El primero de ellos se centra en obtener la máxima potencia lumínica, con valores comprendidos entre 40W y 50W, considerando un uso industrial, alumbrado público, etc. El segundo rango de potencias, se enfocará al ámbito doméstico, adecuando la temperatura de color a este entorno, para combinar la potencia, de entre 9W y 12W, con una iluminación más cálida. Finalmente someteremos los prototipos elaborados a una serie de análisis y, ante los resultados, concluiremos cuales serán aptos para el uso al que se pretenden dedicar y aquellos que deberán ser modificados. En el entorno de laboratorio, donde se llevaran a cabo análisis térmicos, eléctricos y lumínicos, emplearemos instrumental especializado para cada tipo de análisis así como su software correspondiente. A excepción de las mediciones relacionadas con el espectro de radiación lumínica, el resto serán obtenidas mediante la plataforma LabVIEW®, un completo software grafico para el desarrollo de instrumentación virtual. Como sección final, expondremos una evaluación sobre el cumplimiento de los objetivos establecidos para el proyecto, posibles mejoras o trabajos futuros y conclusiones obtenidas. ABSTRACT. This project concerns the design and analysis, with its different stages, of various configurations of LED luminaries prototypes focused on domestic and industrial environments. Mounts on different materials will be made to evaluate the results and decide which material is most appropriate for each circumstance. Initially we will expose the current state of this technology and characterize, in a preliminary way, the devices that we will use to produce the prototypes which subsequently will analyze. Later we detail the process of construction of each prototype, especially indicating the differences and similarities between them. In the following sections, we will analyze these characteristics. We will work with two power ranges for luminaries. The first one focuses on maximum light output, with values between 40W and 50W, thinking about industrial use, street lighting, etc. The second power range will focus on the domestic environment, adjusting the color temperature in this environment to combine power, between 9W and 12W, with warm lighting. Finally the prototypes will submit a series of analyzes and, with the results obtained, we conclude if will be suitable for the intended use and those that should be modified. In laboratory environment, where thermal, electrical and lighting analyzes were carried out, we will use specialized instruments for each type of analysis as well as the corresponding software. Except for measurements related to the spectrum of light radiation, the rest of information will be obtained by LabVIEW®, complete graphical development software for virtual instrumentation. As a final section, we will present an evaluation of compliance with the project objectives, possible future work, or improvements, and conclusions.

Spectrally tunable light source based on light-emitting diodes for custom lighting solutions

This study describes a novel spectral LED-based tunable light source used for customized lighting solutions, especially for the reconstruction of CIE (Commission Internationale de l’Éclairage) standard illuminants. The light source comprises 31 spectral bands ranging from 400 to 700 nm, an integrating cube and a control board with a 16-bit resolution. A minimization algorithm to calculate the weighting values for each channel was applied to reproduce illuminants with precision. The differences in spectral fitting and colorimetric parameters showed that the reconstructed spectra were comparable to the standard, especially for the D65, D50, A and E illuminants. Accurate results were also obtained for illuminants with narrow peaks such as fluorescents (F2 and F11) and a high-pressure sodium lamp (HP1). In conclusion, the developed spectral LED-based light source and the minimization algorithm are able to reproduce any CIE standard illuminants with a high spectral and colorimetric accuracy able to advance available custom lighting systems useful in the industry and other fields such as museum lighting.

MOVPE growth and characterization of Al(Ga)N and InAlN/AlGaN quantum wells for UV LED applications

The study of III-nitride materials (InN, GaN and AlN) gained huge research momentum after breakthroughs in the production light emitting diodes (LEDs) and laser diodes (LDs) over the past two decades. Last year, the Nobel Prize in Physics was awarded jointly to Isamu Akasaki, Hiroshi Amano and Shuji Nakamura for inventing a new energy efficient and environmental friendly light source: blue light-emitting diode (LED) from III-nitride semiconductors in the early 1990s. Nowadays, III-nitride materials not only play an increasingly important role in the lighting technology, but also become prospective candidates in other areas, for example, the high frequency (RF) high electron mobility transistor (HEMT) and photovoltaics. These devices require the growth of high quality III-nitride films, which can be prepared using metal organic vapour phase epitaxy (MOVPE). The main aim of my thesis is to study and develop the growth of III-nitride films, including AlN, u-AlGaN, Si-doped AlGaN, and InAlN, serving as sample wafers for fabrication of ultraviolet (UV) LEDs, in order to replace the conventional bulky, expensive and environmentally harmful mercury lamp as new UV light sources. For application to UV LEDs, reducing the threading dislocation density (TDD) in AlN epilayers on sapphire substrates is a key parameter for achieving high-efficiency AlGaNbased UV emitters. In Chapter 4, after careful and systematic optimisation， a working set of conditions, the screw and edge type dislocation density in the AlN were reduced to around 2.2×108 cm-2 and 1.3×109 cm-2 , respectively, using an optimized three-step process, as estimated by TEM. An atomically smooth surface with an RMS roughness of around 0.3 nm achieved over 5×5 µm 2 AFM scale. Furthermore, the motion of the steps in a one dimension model has been proposed to describe surface morphology evolution, especially the step bunching feature found under non-optimal conditions. In Chapter 5, control of alloy composition and the maintenance of compositional uniformity across a growing epilayer surface were demonstrated for the development of u-AlGaN epilayers. Optimized conditions (i.e. a high growth temperature of 1245 °C) produced uniform and smooth film with a low RMS roughness of around 2 nm achieved in 20×20 µm 2 AFM scan. The dopant that is most commonly used to obtain n-type conductivity in AlxGa1-xN is Si. However, the incorporation of Si has been found to increase the strain relaxation and promote unintentional incorporation of other impurities (O and C) during Si-doped AlGaN growth. In Chapter 6, reducing edge-type TDs is observed to be an effective appoach to improve the electric and optical properties of Si-doped AlGaN epilayers. In addition, the maximum electron concentration of 1.3×1019 cm-3 and 6.4×1018 cm-3 were achieved in Si-doped Al0.48Ga0.52N and Al0.6Ga0.4N epilayers as measured using Hall effect. Finally, in Chapter 7, studies on the growth of InAlN/AlGaN multiple quantum well (MQW) structures were performed, and exposing InAlN QW to a higher temperature during the ramp to the growth temperature of AlGaN barrier (around 1100 °C) will suffer a significant indium (In) desorption. To overcome this issue, quasi-two-tempeature (Q2T) technique was applied to protect InAlN QW. After optimization, an intense UV emission from MQWs has been observed in the UV spectral range from 320 to 350 nm measured by room temperature photoluminescence.

Street lighting mesh network protocol

The digital revolution of the 21st century contributed to stem the Internet of Things (IoT). Trillions of embedded devices using the Internet Protocol (IP), also called smart objects, will be an integral part of the Internet. In order to support such an extremely large address space, a new Internet Protocol, called Internet Protocol Version 6 (IPv6) is being adopted. The IPv6 over Low Power Wireless Personal Area Networks (6LoWPAN) has accelerated the integration of WSNs into the Internet. At the same time, the Constrained Application Protocol (CoAP) has made it possible to provide resource constrained devices with RESTful Web services functionalities. This work builds upon previous experience in street lighting networks, for which a proprietary protocol, devised by the Lighting Living Lab, was implemented and used for several years. The proprietary protocol runs on a broad range of lighting control boards. In order to support heterogeneous applications with more demanding communication requirements and to improve the application development process, it was decided to port the Contiki OS to the four channel LED driver (4LD) board from Globaltronic. This thesis describes the work done to adapt the Contiki OS to support the Microchip TM PIC24FJ128GA308 microprocessor and presents an IP based solution to integrate sensors and actuators in smart lighting applications. Besides detailing the system’s architecture and implementation, this thesis presents multiple results showing that the performance of CoAP based resource retrievals in constrained nodes is adequate for supporting networking services in street lighting networks.