Synergistic effect of UVB radiation and age on HMPS enzymes in rat lens homogenate

The behaviour of rat lenticular enzymes, glucose-6-phosphate dehydrogena.se (G6PD, EC: 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGD, EC: 1.1.1.44) as a function of age and UVB irradiation (in vitro) was investigated by irradiating the lens homogenate from 3-and 12-month-old rats at 300 nm (100 μW cm 2). In the 3-month-old group the specific activities of G6PD and 6PGD were reduced by 26% and 42%, respectively, after 24 h of irradiation, whereas in the 12-month-old group the decrease was 38% and 49% respectively, which suggests that the susceptibility of HMPS enzymes to UVB damage is higher in older lenses. The decrease in specitic activity was associated with a change in apparent Km and Vmax (marginal in 3 months and significant in 12 months) of these enzymes due to UVB irradiation. UVB irradiation also decreased the levels of NADPH and NADPH/NADP ratio. These changes, altered activities of G6PD and 6PGD and altered levels of NADPH. may in turn have a bearing on lens transparency.

Patent intelligence and its implications for patent productivity

The prevailing hypercompetitive environment has made it essential for organizations to gather competitive intelligence from environmental scanning. The knowledge gained leads to organizational learning, which stimulates increased patent productivity. This paper highlights five practices that aid in developing patenting intelligence and empirically verifies to what extent this organizational learning leads to knowledge gains and financial gains realized from consequent higher patent productivity. The model is validated based on the perceptions of professionals with patenting experience from two of the most aggressively patenting sectors in today’s economy, viz., IT and pharmaceutical sectors (n=119). The key finding of our study suggests that although organizational learning from environmental scanning exists, the application of this knowledge for increasing patent productivity lacks due appreciation. This missing link in strategic analysis and strategy implementation has serious implications for managers which are briefly discussed in this paper.

Age determination and age related factors in the teeth of Western North Atlantic bottlenose dolphins.

Teeth were taken from 120 bottlenose dolphins, Tursiops truncatus, which had stranded on the mid-Atlantic coast of the United States. The number of annual growth layer groups (GLGs) for each animal was used to construct a growth curve. The growth rate of coastal North Atlantic Ocean Tursiops is similar to other cetaceans in having a high initial rate of growth, with no differences in growth between females and males. In females, the first dentinal GLG is thickest and is followed by GLGs which become progressively narrower. In males, the second GLG is thicker than the first; GLGs beyond number two become progressively smaller but at a slower rate than in females. In males and females, the translucent layer makes up proportionally larger parts of the GLG as the animal ages, but in males the percent translucent layer remains constant at about 50% while in females it continues to increase up to about 70% of the GLG. These two factors, GLGs width and translucent layer width, indicate that the sex and age of the animal influence the deposition of GLGs. Incremental layers are also present, averaging 12 per GLG, and seem similar to incremental layers described in other marine mammals. A plot of the relationship of percent growth of the last GLG to time of death suggests that the deposition of GLGs is relatively constant, at least during the first half of the year, and that North Atlantic Ocean Tursiops give birth in the fall as well as in the spring. (PDF contains 31 pages.)

Growth and age composition of northern bluefin tuna, Thunnus thynnus, caught in the Eastern Pacific Ocean, as estimated from length-frequency data, with comments on trans-Pacific migrations

ENGLISH: The spawning of Pacific northern bluefin tuna, Thunnus thynnus, takes place only in the western Pacific Ocean (WPO), but substantial numbers of the juveniles migrate to the eastern Pacific Ocean (EPO), where they remain for several months, or longer, and the.n return to the WPO. Lengthfrequency and tagging data show that many bluefin arrive in the EPO as 1-and 2-year olds, and remain there for one or two fishing seasons before returning to the WPO. The proportion of the fish which make the west-to-east migration varies among years. The numbers of 1-, 2-, 3-, 4, and >4 –year olds in the catches of the EPO are estimated for most years of the 1952-1991 period. SPANISH: EI desove del atun aleta azul del norte del Pacifico, Thunnus thynnus, ocurre solamente en el Océano Pacifico occidental (WPO), pero números substanciales de los juveniles migran al Océano Pacifico oriental (OPO), donde permanecen unos meses, 0 mas, antes de regresar al WPO. Datos de marcado y frecuencia de talla indican que muchos aletas azules llegan al OPO a 1 o 2 anos de edad, y permanecen alIi una 0 dos temporadas de pesca antes de regresar al WPO. La proporcion de los peces que migra del oeste al este varia entre anos. Se estima el numero de peces de 1, 2, 3, 4, Y>4 anos de edad en las capturas del OPO para la mayoria de los anos del periodo de 1952-1991. (PDF contains 40 pages.)

Changes in catch rates and length and age at maturity, but not growth, of an estuarine plotosid (Cnidoglanis macrocephalus) after heavy fishing

The hypothesis that heavy fishing pressure has led to changes in the biological characteristics of the estuary cobbler (Cnidoglanis macrocephalus) was tested in a large seasonally open estuary in southwestern Australia, where this species completes its life cycle and is the most valuable commercial fish species. Comparisons were made between seasonal data collected for this plotosid (eeltail catfish) in Wilson Inlet during 2005–08 and those recorded with the same fishery-independent sampling regime during 1987–89. These comparisons show that the proportions of larger and older individuals and the catch rates in the more recent period were far lower, i.e., they constituted reductions of 40% for fish ≥430 mm total length, 62% for fish ≥4 years of age, and 80% for catch rate. In addition, total mortality and fishing-induced mortality estimates increased by factors of ~2 and 2.5, respectively. The indications that the abundance and proportion of older C. macrocephalus declined between the two periods are consistent with the perception of long-term commercial fishermen and their shift toward using a smaller maximum gill net mesh to target this species. The sustained heavy fishing pressure on C. macrocephalus between 1987–89 and 2005–08 was accompanied by a marked reduction in length and age at maturity of this species. The shift in probabilistic maturation reaction norms toward smaller fish in 2005–08 and the lack of a conspicuous change in growth between the two periods indicate that the maturity changes were related to fishery-induced evolution rather than to compensatory responses to reduced fish densities.

A comparison of length-related and age-related growth parameters of newaiby Otolithes ruber in Kuwait waters

The growth parameters of Otolithes ruber (Sciaenidae) were determined from both length-frequency and length-at-age data collected from Kuwait waters from 1984 to 1986. The similarity of the growth parameters is reflected in the small range of the parameters o' (=log sub(10)K+2logL) which indicates the compatibility of the two methods for this relatively short-lived species.

Estimates of growth and comparisons of growth rates determined from length- and age-based models for populations of purple wrasse (Notolabrus fucicola)

Growth of a temperate reefa-ssociated fish, the purple wrasse (Notolabrus fucicola), was examined from two sites on the east coast of Tasmania by using age- and length-based models. Models based on the von Bertalanffy growth function, in the standard and a reparameterized form, were constructed by using otolith-derived age estimates. Growth trajectories from tag-recaptures were used to construct length-based growth models derived from the GROTAG model, in turn a reparameterization of the Fabens model. Likelihood ratio tests (LRTs) determined the optimal parameterization of the GROTAG model, including estimators of individual growth variability, seasonal growth, measurement error, and outliers for each data set. Growth models and parameter estimates were compared by bootstrap confidence intervals, LRTs, and randomization tests and plots of bootstrap parameter estimates. The relative merit of these methods for comparing models and parameters was evaluated; LRTs combined with bootstrapping and randomization tests provided the most insight into the relationships between parameter estimates. Significant differences in growth of purple wrasse were found between sites in both length- and age-based models. A significant difference in the peak growth season was found between sites, and a large difference in growth rate between sexes was found at one site with the use of length-based models.

Review of size- and age-dependence in batch spawning: implications for stock assessment of fish species exhibiting indeterminate fecundity

Most assessments of fish stocks use some measure of the reproductive potential of a population, such as spawning biomass. However, the correlation between spawning biomass and reproductive potential is not always strong, and it likely is weakest in the tropics and subtropics, where species tend to exhibit indeterminate fecundity and release eggs in batches over a protracted spawning season. In such cases, computing annual reproductive output requires estimates of batch fecundity and the annual number of batches—the latter subject to spawning frequency and duration of spawning season. Batch fecundity is commonly measured by age (or size), but these other variables are not. Without the relevant data, the annual number of batches is assumed to be invariant across age. We reviewed the literature and found that this default assumption lacks empirical support because both spawning duration and spawning frequency generally increase with age or size. We demonstrate effects of this assumption on measures of reproductive value and spawning potential ratio, a metric commonly used to gauge stock status. Model applications showed substantial sensitivity to age dependence in the annual number of batches. If the annual number of batches increases with age but is incorrectly assumed to be constant, stock assessment models would tend to overestimate the biological reference points used for setting harvest rates. This study underscores the need to better understand the age- or size-dependent contrast in the annual number of batches, and we conclude that, for species without evidence to support invariance, the default assumption should be replaced with one that accounts for age- or size-dependence.

A comparison of size and age of red snapper (Lutjanus campechanus) with the age of artificial reefs in the northern Gulf of Mexico

Despite extensive study, it still is not clear whether artificial reefs produce new fish biomass or whether they only attract various species and make them more vulnerable to fishing mortality. To further evaluate this question, the size and age of red snapper (Lutjanus campechanus) were sampled from April to November 2010 at artificial reefs south of Mobile Bay off the coast of Alabama and compared with the age of the artificial reef at the site of capture. Red snapper were collected with hook and line and a fish trap and visually counted during scuba-diver surveys. In the laboratory, all captured red snapper were weighed and measured, and the otoliths were removed for aging. The mean age of red snapper differed significantly across reefs of different ages, with older reefs having older fish. The mean age of red snapper at a particular reef was not related to reef depth or distance to other reefs. The positive correlation between the mean age of red snapper and the age of the reef where they were found supports the contention that artificial reefs in the northern Gulf of Mexico enhance production of red snapper. The presence of fish older than the reef indicates that red snapper are also attracted to artificial reefs.

Changes in size and age at maturity of the northern stock of Tilefish (Lopholatilus chamaeleonticeps) after a period of overfishing

The modern fishery for Tilefish (Lopholatilus chamaeleonticeps) developed during the 1970s, offshore of southern New England, in the western North Atlantic Ocean. The population quickly became over exploited, with documented declines in catch rates and changes in demographic traits. In an earlier study, median size at maturity (L50) of males declined from 62.6 to 38.6 cm fork length (FL) and median age at maturity (A50) of males declined from 7.1 to 4.6 years between 1978 and 1982. As part of a cooperative research effort to improve the data-limited Tilefish assessment, we updated maturity parameter estimates through the use of an otolith aging method and macroscopic and microscopic evaluations of gonads. The vital rates for this species have continued to change, particularly for males. By 2008, male L50 and A50 had largely rebounded, to 54.1 cm FL and 5.9 years. Changes in female reproductive schedules were less variable among years, but the smallest L50 and youngest A50 were recorded in 2008. Tilefish are dimorphic, where the largest fish are male, and male spawning success is postulated to be socially mediated. These traits may explain the initial rapid decline and the subsequent rebound in male L50 and A50 and less dramatic effects on females. Other factors that likely contribute to the dynamics of maturity parameter estimates are the relatively short period of overfishing and the amount of time since efforts to rebuild this fishery began, as measured in numbers of generations. This study also confirms the gonochoristic sexual pattern of the northern stock, and it reveals evidence of age truncation and relatively high proportions of immature Tilefish in the recent catch.