Samuel Ros : post mortem.

Evocación de la personalidad del escritor Samuel Ros y de su nostalgia por España durante el período en que estuvo evacuado en Chile.

Fotografía e historia. Nuevos enfoques sobre la fotografía post mortem en la representación del cadáver del Presidente García Moreno

En esta tesis se desarrolló un análisis ampliado del álbum de fotografías post mortem del Presidente Gabriel García Moreno, realizado por el fotógrafo ecuatoriano Rafael Pérez Pinto en el año de 1875.Utilizando los aportes y las posibilidades metodológicas y categoriales que ofrece el campo de los estudios visuales para la investigación alrededor de temas relacionados con la imagen. El trabajo consistió en desarrollar un análisis del álbum de fotografías, a partir de un esquema que considera varios niveles de reflexión y ha sido diseñado específicamente para la imagen fotográfica. Así mismo y bajo la categoría de visualidad, se analiza con mayor detenimiento el período histórico-visual al que pertenecen las fotografías y finalmente, se considera el análisis fenomenológico de la imagen mortuoria como aporte fundamental del presente trabajo. La propuesta metodológica consiste en analizar las imágenes de manera individual y considerándolas también como parte un álbum, es decir, como un conjunto, a partir del cual se desprenden los análisis posteriores: El primero que tiene que ver con los usos y funciones de la imagen fotográfica durante el período garciano, considerando que se trata de un período de mayor desarrollo de la fotografía en el país y la relación directa que se establece entre las imágenes y la histórica muerte de García Moreno. Finalmente, la reflexión de corte fenomenológico, que consiste en buscar más allá de la fotografía, el origen de las prácticas en las que la muerte se convierte en el motivo de la representación. La relación íntima que se plantea entre imagen y muerte permite entender el álbum de fotografías post mortem en un contexto más visual y entiendo de mejor manera los elementos del mundo simbólico que pueden ser observados en él.

Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions

Ancient DNA (aDNA) research has long depended on the power of PCR to amplify trace amounts of surviving genetic material from preserved specimens. While PCR permits specific loci to be targeted and amplified, in many ways it can be intrinsically unsuited to damaged and degraded aDNA templates. PCR amplification of aDNA can produce highly-skewed distributions with significant contributions from miscoding lesion damage and non-authentic sequence artefacts. As traditional PCR-based approaches have been unable to fully resolve the molecular nature of aDNA damage over many years, we have developed a novel single primer extension (SPEX)-based approach to generate more accurate sequence information. SPEX targets selected template strands at defined loci and can generate a quantifiable redundancy of coverage; providing new insights into the molecular nature of aDNA damage and fragmentation. SPEX sequence data reveals inherent limitations in both traditional and metagenomic PCR-based approaches to aDNA, which can make current damage analyses and correct genotyping of ancient specimens problematic. In contrast to previous aDNA studies, SPEX provides strong quantitative evidence that C U-type base modifications are the sole cause of authentic endogenous damage-derived miscoding lesions. This new approach could allow ancient specimens to be genotyped with unprecedented accuracy.

Water-soluble precursors of beef flavour. Part II: effect of post-mortem conditioning

Changes in glycolytic metabolites, nucleotide degradation products, free amino acids and other amino compounds were monitored in beef muscle (M. longissimus lumborum), stored for 21 days at 4 degrees C, in order to evaluate how post-mortem conditioning may affect flavour formation in beef. The major effects observed in sugar-related substances were the dephosphorylation of the phosphates of glucose, fructose and mannose, to yield their free sugars, as well as the breakdown of inosine 5'-monophosphate, to give a sixfold increase in ribose. Total reducing sugars increased by only 15% during conditioning, while glycogen levels remained unchanged from 2 days post-slaughter. Free amino acids increased during conditioning, particularly between days 7 and 14. Phenylalanine, methionine, lysine, leucine and isoleucine were the amino acids showing the greatest increase with conditioning time, with methionine, in particular, showing a sevenfold increase during the conditioning period. The effects of these precursor changes on cooked beef flavour are discussed. (c) 2007 Elsevier Ltd. All rights reserved.

Reverências à vida terrena e post-mortem (Caicó-RN, século XIX)

ARAÚJO, M. M. ; Olivia M. de Medeiros Neta ; FIGUEIREDO, Franselma. Reverências à vida terrena e post-mortem (Caicó-RN, século XIX). Revista HISTEDBR On-line, v. 33, p. 179-193, mar. 2009

Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.

POST-MORTEM LESIONS of UROLITHIASIS IN A LESSER SEED FINCH (Sporophila angolensis).

Urolithiasis is a disease that despite being a commonly observed problem in veterinary practice is uncommon in birds. Such disease was not reported in passeriforms to date. Accordingly, the aim of the present article is to describe a case of urolithiasis in an adult female lesser seed finch (Sporophila angolensis) pet bird which presented abdominal distension, respiratory distress, and apathy prior to death. The bird had history of being fed with a diet rich in protein. After the bird death, a necropsy was conducted in order to determine the cause of death. At necropsy, accentuated ascites, hydropericardium, and ureteral stones in the left ureter could be grossly observed. Additional tests related with viral and bacterial microbiological testing and with the determination of calculi composition could not be performed since the owner did not consent with the procedures because of the cost. Since the bird was fed on a high protein diet, a relationship between the ureteroliths and dietary imbalance was suggested with participation of protein in calculi development by providing the organic nuclei. Additionally, we conclude that the presence of calculi in the ureter resulted in urinary flow blockage, ascites, and consequent acute respiratory failure due to filling of air sacs with liquid.

Ocorrência de lesões hepáticas provocadas por larvas de Strongylus spp diagnosticadas no exame post mortem de eqüídeos abatidos em Apucarana, Paraná

The objective of this study was to determinate the occurrence of hepatic lesions caused by migratory large Strongyle larvaes in horses slaughtered in city of Apucarana in state of Parana. The lesions were diagnostic in post mortem exam by macro and microscopic analysis. From April 2003 to April 2004,38,363 animals, coming from different regions of Brazil, were examined. The occurrence of granulomas in liver was observed in 14,443 (37.64%), with adhesions and spot in 6,645 (17,32%), and 17,275 (45.03%) without macroscopic lesions. Macroscopic analysis revealed the presence of livers with calcified nodules, the presence of whitish spots, and adhesions in the format of "lines" over the hepatic capsule. No larvae were found in the livers. Hepatic fragments were histologically processed and revealed, by optical microscopy, inflammatory cells with predominance of eosinophils around the granulomas with a moderate amount of macrophages and the presence of fibroblasts.

A standard protocol for obtaining fish chromosomes under post-mortem conditions

In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed. (c) 2006 Elsevier Ltd. All rights reserved.

Efeito do resfriamento sobre a textura post-mortem da carne do peixe matrinxã Brycon cephalus

Os mecanismos que causam o amolecimento e a perda na textura post-mortem da carne de matrinxã foram determinados por meio das mudanças na microestrutura do músculo, imediatamente após a morte e depois de 12 horas de estocagem a -3°C. As observações na microestrutura, realizadas com microscópio eletrônico de transmissão, foram semelhantes aos resultados obtidos na força de ruptura do músculo medidos com o texturômetro. Os valores da força da ruptura foram menores para a carne após o resfriamento. Observou-se que as fibras do colágeno do tecido conectivo pericelular se desintegraram e que as do colágeno do tecido conectivo do miocommata conservaram sua arquitetura e integridade. Houve pouca degradação da linha Z. Isso sugere que o amolecimento post-mortem da carne de mantrinxã, durante a estocagem a -3°C, é causado pela degradação do tecido conectivo pericelular.

Post mortem, citologia, histopatologia e bacteriologia no diagnóstico da tuberculose bovina - matadouro-frigorífico da Região Norte do Paraná

Pós-graduação em Medicina Veterinária - FMVZ