Characterization of Recombinant Rhamnogalacturonan α-l-Rhamnopyranosyl-(1,4)-α-d-Galactopyranosyluronide Lyase from Aspergillus aculeatus1 : An Enzyme That Fragments Rhamnogalacturonan I Regions of Pectin

The four major oligomeric reaction products from saponified modified hairy regions (MHR-S) from apple, produced by recombinant rhamnogalacturonan (RG) α-l-rhamnopyranosyl-(1,4)-α-d-galactopyranosyluronide lyase (rRG-lyase) from Aspergillus aculeatus, were isolated and characterized by 1H-nuclear magnetic resonance spectroscopy. They contain an alternating RG backbone with a degree of polymerization of 4, 6, 8, and 10 and with an α-Δ-(4,5)-unsaturated d-galactopyranosyluronic acid at the nonreducing end and an l-rhamnopyranose at the reducing end. l-Rhamnopyranose units are substituted at C-4 with β-galactose. The maximum reaction rate of rRG-lyase toward MHR-S at pH 6.0 and 31°C was 28 units mg−1. rRG-lyase and RG-hydrolase cleave the same alternating RG I subunit in MHR. Both of these enzymes fragment MHR by a multiple attack mechanism. The catalytic efficiency of rRG-lyase for MHR increases with decreasing degree of acetylation. Removal of arabinose side chains improves the action of rRG-lyase toward MHR-S. In contrast, removal of galactose side chains decreased the catalytic efficiency of rRG-lyase. Native RG-lyase was purified from A. aculeatus, characterized, and found to be similar to the rRG-lyase expressed in Aspergillus oryzae.

La Région Méditerranéenne en 2020 et son Rôle dans le Réseau Energétjgue Européen, Vol. I: Analyse de la demande d'energie dans les PSEM (Horizons 2000-2010-2020)= The Mediterranean region in 2020 and its Role in the European Energy Network, Vol. I: Analysis of the energy demand in the SEMC (Horizons 2000-2010-2020).

None.

Test facility inventory for the U.S. Department of Transportation. Final report. Volume III of VI. D-I.

National Highway Safety Bureau, Washington, D.C.

A dream of John Ball : and A king's lesson /

"A dream of John Ball" first appeared in the "Commonweal", 1886-87; issued in book-form, 1888. "A king's lesson", a story based on the life of Matthias Corvinus, also appeared in the "Commonweal", Sept. 18, 1886.

New poems by James I of England : from a hitherto unpublished manuscript (Add. 24195) in the British museum /

Vita.

Chronica de el-rei D. João I,

Edited by Luciano Cordeiro.

Attila, king of the Huns.

A "supplement containing above six hundred additional lines to Attila books VIII, XI, and XII" was appended to the author's Works, 1842, p. [555]-580.

Per le faustissime nozze di S.A.R. il duca di Savoja Vittorio Emanuele con S.A.I.R. l'arciduchessa d'Austria Maria Adelaide : discorso ... /

Mode of access: Internet.

Oração gratularia pelas melhoras e feliz restabelecimento de sua magestade El Rei Nosso Senhor, O Senhor D. Miguel I, recitada em solemne acção de graças que endereçou ao todo poderoso em o dia 11 de Janeiro de 1829, a religiosa comunidade de S. Francisco de Xabregas /

With: Sermão prégado na sé de angra por occasião do Te Deum que se cantou no dia 4 de abril, anniversario natalicio de S.M.F. a senhora Dona Maria Segunda, Rainha de Portugal, na augusta presença de S.M.I. o senhor Duque de Bragança, Regente do Reino em nome da rainha / por Marcos Pinto Soares Vaz Preto. -- Lisboa : Imprensa Nacional, 1833 -- 22 p. ; 20 cm.

D. Miguel I., obra a mais completa e concludente que tem apparecido na Europa sobre a legitimidade e inauferiveis direitos do senhor D. Miguel I. ao throno de Portugal.

Translation attributed to Fr. João de S. Boaventura. cf. E. do Canto's Catalogo das obras nacionaes e estrangeiras relativas aos successos politicos de Portugal nos annos de 1828 a 1834, 2. ed., p. 213.

Iscrizioni ed orazione nei solenni funerali di S.M. Ferdinando I, re del regno delle due Sicilie, nel dì 27 gennajo 1825 /

Mode of access: Internet.