In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction

Recurrent airway obstruction (RAO) is a common condition in stabled horses characterised by small airway inflammation, airway neutrophilia and obstruction following exposure of susceptible horses to mouldy hay and straw and is thus regarded as a hypersensitivity reaction to mould spores. However, the role of IgE-mediated reactions in RAO remains unclear. The aim of the study was to investigate with a serological IgE ELISA test (Allercept), an in vitro sulfidoleukotriene (sLT) release assay (CAST) and with intradermal testing (IDT) whether serum IgE and IgE-mediated reactions against various mould, mite and pollen extracts are associated with RAO. IDT reactions were evaluated at different times in order to detect IgE-mediated immediate type reactions (type I hypersensitivity reactions, 0.5-1 h), immune complex-mediated late type reactions (type III reactions, 4-10 h) and cell-mediated delayed type reactions (type IV hypersensitivity reactions 24-48 h). In the serological test, overall the control horses displayed more positive reactions than the RAO-affected horses but the difference was not significant. Comparison of the measured IgE levels showed that the RAO-affected horses had slightly higher IgE levels against Aspergillus fumigatus than controls (35 and 16 AU, respectively, p<0.05), but all values were below the cut off (150 AU) of the test. In the sLT release assay, seven positive reactions were observed in the RAO-affected horses and four in the controls but this difference was not significant. A significantly higher proportion of late type IDT reactions was observed in RAO-affected horses compared to controls (25 of 238 possible reactions versus 12 of 238 possible reactions, respectively, p<0.05). Interestingly, four RAO-affected but none of the control horses reacted with the recombinant mould allergen A. fumigatus 8 (rAsp f 8, p<0.05), but only late phase and delayed type reactions were observed. In all three tests the majority of the positive reactions was observed with the mite extracts (64%, 74% and 88% of all positive reactions, respectively) but none of the tests showed a significant difference between RAO-affected and control animals. Our findings do not support that IgE-mediated reactions are important in the pathogenesis of RAO. Further studies are needed to investigate whether sensitisation to mite allergens is of clinical relevance in the horse and to understand the role of immune reactions against rAsp f 8.

Análisis de los factores determinantes de la repetición del proceso de compra en el comercio electrónico entre empresas y consumidores finales. Aplicación al mercado español

Las Tecnologías de la Información y la Comunicación en general e Internet en particular han supuesto una revolución en nuestra forma de comunicarnos, relacionarnos, producir, comprar y vender acortando tiempo y distancias entre proveedores y consumidores. A la paulatina penetración del ordenador, los teléfonos inteligentes y la banda ancha fija y/o móvil ha seguido un mayor uso de estas tecnologías entre ciudadanos y empresas. El comercio electrónico empresa–consumidor (B2C) alcanzó en 2010 en España un volumen de 9.114 millones de euros, con un incremento del 17,4% respecto al dato registrado en 2009. Este crecimiento se ha producido por distintos hechos: un incremento en el porcentaje de internautas hasta el 65,1% en 2010 de los cuales han adquirido productos o servicios a través de la Red un 43,1% –1,6 puntos porcentuales más respecto a 2010–. Por otra parte, el gasto medio por comprador ha ascendido a 831€ en 2010, lo que supone un incremento del 10,9% respecto al año anterior. Si segmentamos a los compradores según por su experiencia anterior de compra podemos encontrar dos categorías: el comprador novel –que adquirió por primera vez productos o servicios en 2010– y el comprador constante –aquel que había adquirido productos o servicios en 2010 y al menos una vez en años anteriores–. El 85,8% de los compradores se pueden considerar como compradores constantes: habían comprado en la Red en 2010, pero también lo habían hecho anteriormente. El comprador novel tiene un perfil sociodemográfico de persona joven de entre 15–24 años, con estudios secundarios, de clase social media y media–baja, estudiante no universitario, residente en poblaciones pequeñas y sigue utilizando fórmulas de pago como el contra–reembolso (23,9%). Su gasto medio anual ascendió en 2010 a 449€. El comprador constante, o comprador que ya había comprado en Internet anteriormente, tiene un perfil demográfico distinto: estudios superiores, clase alta, trabajador y residente en grandes ciudades, con un comportamiento maduro en la compra electrónica dada su mayor experiencia –utiliza con mayor intensidad canales exclusivos en Internet que no disponen de tienda presencial–. Su gasto medio duplica al observado en compradores noveles (con una media de 930€ anuales). Por tanto, los compradores constantes suponen una mayoría de los compradores con un gasto medio que dobla al comprador que ha adoptado el medio recientemente. Por consiguiente es de interés estudiar los factores que predicen que un internauta vuelva a adquirir un producto o servicio en la Red. La respuesta a esta pregunta no se ha revelado sencilla. En España, la mayoría de productos y servicios aún se adquieren de manera presencial, con una baja incidencia de las ventas a distancia como la teletienda, la venta por catálogo o la venta a través de Internet. Para dar respuesta a las preguntas planteadas se ha investigado desde distintos puntos de vista: se comenzará con un estudio descriptivo desde el punto de vista de la demanda que trata de caracterizar la situación del comercio electrónico B2C en España, poniendo el foco en las diferencias entre los compradores constantes y los nuevos compradores. Posteriormente, la investigación de modelos de adopción y continuidad en el uso de las tecnologías y de los factores que inciden en dicha continuidad –con especial interés en el comercio electrónico B2C–, permiten afrontar el problema desde la perspectiva de las ecuaciones estructurales pudiendo también extraer conclusiones de tipo práctico. Este trabajo sigue una estructura clásica de investigación científica: en el capítulo 1 se introduce el tema de investigación, continuando con una descripción del estado de situación del comercio electrónico B2C en España utilizando fuentes oficiales (capítulo 2). Posteriormente se desarrolla el marco teórico y el estado del arte de modelos de adopción y de utilización de las tecnologías (capítulo 3) y de los factores principales que inciden en la adopción y continuidad en el uso de las tecnologías (capítulo 4). El capítulo 5 desarrolla las hipótesis de la investigación y plantea los modelos teóricos. Las técnicas estadísticas a utilizar se describen en el capítulo 6, donde también se analizan los resultados empíricos sobre los modelos desarrollados en el capítulo 5. El capítulo 7 expone las principales conclusiones de la investigación, sus limitaciones y propone nuevas líneas de investigación. La primera parte corresponde al capítulo 1, que introduce la investigación justificándola desde un punto de vista teórico y práctico. También se realiza una breve introducción a la teoría del comportamiento del consumidor desde una perspectiva clásica. Se presentan los principales modelos de adopción y se introducen los modelos de continuidad de utilización que se estudiarán más detalladamente en el capítulo 3. En este capítulo se desarrollan los objetivos principales y los objetivos secundarios, se propone el mapa mental de la investigación y se planifican en un cronograma los principales hitos del trabajo. La segunda parte corresponde a los capítulos dos, tres y cuatro. En el capítulo 2 se describe el comercio electrónico B2C en España utilizando fuentes secundarias. Se aborda un diagnóstico del sector de comercio electrónico y su estado de madurez en España. Posteriormente, se analizan las diferencias entre los compradores constantes, principal interés de este trabajo, frente a los compradores noveles, destacando las diferencias de perfiles y usos. Para los dos segmentos se estudian aspectos como el lugar de acceso a la compra, la frecuencia de compra, los medios de pago utilizados o las actitudes hacia la compra. El capítulo 3 comienza desarrollando los principales conceptos sobre la teoría del comportamiento del consumidor, para continuar estudiando los principales modelos de adopción de tecnología existentes, analizando con especial atención su aplicación en comercio electrónico. Posteriormente se analizan los modelos de continuidad en el uso de tecnologías (Teoría de la Confirmación de Expectativas; Teoría de la Justicia), con especial atención de nuevo a su aplicación en el comercio electrónico. Una vez estudiados los principales modelos de adopción y continuidad en el uso de tecnologías, el capítulo 4 analiza los principales factores que se utilizan en los modelos: calidad, valor, factores basados en la confirmación de expectativas –satisfacción, utilidad percibida– y factores específicos en situaciones especiales –por ejemplo, tras una queja– como pueden ser la justicia, las emociones o la confianza. La tercera parte –que corresponde al capítulo 5– desarrolla el diseño de la investigación y la selección muestral de los modelos. En la primera parte del capítulo se enuncian las hipótesis –que van desde lo general a lo particular, utilizando los factores específicos analizados en el capítulo 4– para su posterior estudio y validación en el capítulo 6 utilizando las técnicas estadísticas apropiadas. A partir de las hipótesis, y de los modelos y factores estudiados en los capítulos 3 y 4, se definen y vertebran dos modelos teóricos originales que den respuesta a los retos de investigación planteados en el capítulo 1. En la segunda parte del capítulo se diseña el trabajo empírico de investigación definiendo los siguientes aspectos: alcance geográfico–temporal, tipología de la investigación, carácter y ambiente de la investigación, fuentes primarias y secundarias utilizadas, técnicas de recolección de datos, instrumentos de medida utilizados y características de la muestra utilizada. Los resultados del trabajo de investigación constituyen la cuarta parte de la investigación y se desarrollan en el capítulo 6, que comienza analizando las técnicas estadísticas basadas en Modelos de Ecuaciones Estructurales. Se plantean dos alternativas, modelos confirmatorios correspondientes a Métodos Basados en Covarianzas (MBC) y modelos predictivos. De forma razonada se eligen las técnicas predictivas dada la naturaleza exploratoria de la investigación planteada. La segunda parte del capítulo 6 desarrolla el análisis de los resultados de los modelos de medida y modelos estructurales construidos con indicadores formativos y reflectivos y definidos en el capítulo 4. Para ello se validan, sucesivamente, los modelos de medida y los modelos estructurales teniendo en cuenta los valores umbrales de los parámetros estadísticos necesarios para la validación. La quinta parte corresponde al capítulo 7, que desarrolla las conclusiones basándose en los resultados del capítulo 6, analizando los resultados desde el punto de vista de las aportaciones teóricas y prácticas, obteniendo conclusiones para la gestión de las empresas. A continuación, se describen las limitaciones de la investigación y se proponen nuevas líneas de estudio sobre distintos temas que han ido surgiendo a lo largo del trabajo. Finalmente, la bibliografía recoge todas las referencias utilizadas a lo largo de este trabajo. Palabras clave: comprador constante, modelos de continuidad de uso, continuidad en el uso de tecnologías, comercio electrónico, B2C, adopción de tecnologías, modelos de adopción tecnológica, TAM, TPB, IDT, UTAUT, ECT, intención de continuidad, satisfacción, confianza percibida, justicia, emociones, confirmación de expectativas, calidad, valor, PLS. ABSTRACT Information and Communication Technologies in general, but more specifically those related to the Internet in particular, have changed the way in which we communicate, relate to one another, produce, and buy and sell products, reducing the time and shortening the distance between suppliers and consumers. The steady breakthrough of computers, Smartphones and landline and/or wireless broadband has been greatly reflected in its large scale use by both individuals and businesses. Business–to–consumer (B2C) e–commerce reached a volume of 9,114 million Euros in Spain in 2010, representing a 17.4% increase with respect to the figure in 2009. This growth is due in part to two different facts: an increase in the percentage of web users to 65.1% en 2010, 43.1% of whom have acquired products or services through the Internet– which constitutes 1.6 percentage points higher than 2010. On the other hand, the average spending by individual buyers rose to 831€ en 2010, constituting a 10.9% increase with respect to the previous year. If we select buyers according to whether or not they have previously made some type of purchase, we can divide them into two categories: the novice buyer–who first made online purchases in 2010– and the experienced buyer: who also made purchases in 2010, but had done so previously as well. The socio–demographic profile of the novice buyer is that of a young person between 15–24 years of age, with secondary studies, middle to lower–middle class, and a non–university educated student who resides in smaller towns and continues to use payment methods such as cash on delivery (23.9%). In 2010, their average purchase grew to 449€. The more experienced buyer, or someone who has previously made purchases online, has a different demographic profile: highly educated, upper class, resident and worker in larger cities, who exercises a mature behavior when making online purchases due to their experience– this type of buyer frequently uses exclusive channels on the Internet that don’t have an actual store. His or her average purchase doubles that of the novice buyer (with an average purchase of 930€ annually.) That said, the experienced buyers constitute the majority of buyers with an average purchase that doubles that of novice buyers. It is therefore of interest to study the factors that help to predict whether or not a web user will buy another product or use another service on the Internet. The answer to this question has proven not to be so simple. In Spain, the majority of goods and services are still bought in person, with a low amount of purchases being made through means such as the Home Shopping Network, through catalogues or Internet sales. To answer the questions that have been posed here, an investigation has been conducted which takes into consideration various viewpoints: it will begin with a descriptive study from the perspective of the supply and demand that characterizes the B2C e–commerce situation in Spain, focusing on the differences between experienced buyers and novice buyers. Subsequently, there will be an investigation concerning the technology acceptance and continuity of use of models as well as the factors that have an effect on their continuity of use –with a special focus on B2C electronic commerce–, which allows for a theoretic approach to the problem from the perspective of the structural equations being able to reach practical conclusions. This investigation follows the classic structure for a scientific investigation: the subject of the investigation is introduced (Chapter 1), then the state of the B2C e–commerce in Spain is described citing official sources of information (Chapter 2), the theoretical framework and state of the art of technology acceptance and continuity models are developed further (Chapter 3) and the main factors that affect their acceptance and continuity (Chapter 4). Chapter 5 explains the hypothesis behind the investigation and poses the theoretical models that will be confirmed or rejected partially or completely. In Chapter 6, the technical statistics that will be used are described briefly as well as an analysis of the empirical results of the models put forth in Chapter 5. Chapter 7 explains the main conclusions of the investigation, its limitations and proposes new projects. First part of the project, chapter 1, introduces the investigation, justifying it from a theoretical and practical point of view. It is also a brief introduction to the theory of consumer behavior from a standard perspective. Technology acceptance models are presented and then continuity and repurchase models are introduced, which are studied more in depth in Chapter 3. In this chapter, both the main and the secondary objectives are developed through a mind map and a timetable which highlights the milestones of the project. The second part of the project corresponds to Chapters Two, Three and Four. Chapter 2 describes the B2C e–commerce in Spain from the perspective of its demand, citing secondary official sources. A diagnosis concerning the e–commerce sector and the status of its maturity in Spain is taken on, as well as the barriers and alternative methods of e–commerce. Subsequently, the differences between experienced buyers, which are of particular interest to this project, and novice buyers are analyzed, highlighting the differences between their profiles and their main transactions. In order to study both groups, aspects such as the place of purchase, frequency with which online purchases are made, payment methods used and the attitudes of the purchasers concerning making online purchases are taken into consideration. Chapter 3 begins by developing the main concepts concerning consumer behavior theory in order to continue the study of the main existing acceptance models (among others, TPB, TAM, IDT, UTAUT and other models derived from them) – paying special attention to their application in e–commerce–. Subsequently, the models of technology reuse are analyzed (CDT, ECT; Theory of Justice), focusing again specifically on their application in e–commerce. Once the main technology acceptance and reuse models have been studied, Chapter 4 analyzes the main factors that are used in these models: quality, value, factors based on the contradiction of expectations/failure to meet expectations– satisfaction, perceived usefulness– and specific factors pertaining to special situations– for example, after receiving a complaint justice, emotions or confidence. The third part– which appears in Chapter 5– develops the plan for the investigation and the sample selection for the models that have been designed. In the first section of the Chapter, the hypothesis is presented– beginning with general ideas and then becoming more specific, using the detailed factors that were analyzed in Chapter 4– for its later study and validation in Chapter 6– as well as the corresponding statistical factors. Based on the hypothesis and the models and factors that were studied in Chapters 3 and 4, two original theoretical models are defined and organized in order to answer the questions posed in Chapter 1. In the second part of the Chapter, the empirical investigation is designed, defining the following aspects: geographic–temporal scope, type of investigation, nature and setting of the investigation, primary and secondary sources used, data gathering methods, instruments according to the extent of their use and characteristics of the sample used. The results of the project constitute the fourth part of the investigation and are developed in Chapter 6, which begins analyzing the statistical techniques that are based on the Models of Structural Equations. Two alternatives are put forth: confirmatory models which correspond to Methods Based on Covariance (MBC) and predictive models– Methods Based on Components–. In a well–reasoned manner, the predictive techniques are chosen given the explorative nature of the investigation. The second part of Chapter 6 explains the results of the analysis of the measurement models and structural models built by the formative and reflective indicators defined in Chapter 4. In order to do so, the measurement models and the structural models are validated one by one, while keeping in mind the threshold values of the necessary statistic parameters for their validation. The fifth part corresponds to Chapter 7 which explains the conclusions of the study, basing them on the results found in Chapter 6 and analyzing them from the perspective of the theoretical and practical contributions, and consequently obtaining conclusions for business management. The limitations of the investigation are then described and new research lines about various topics that came up during the project are proposed. Lastly, all of the references that were used during the project are listed in a final bibliography. Key Words: constant buyer, repurchase models, continuity of use of technology, e–commerce, B2C, technology acceptance, technology acceptance models, TAM, TPB, IDT, UTAUT, ECT, intention of repurchase, satisfaction, perceived trust/confidence, justice, feelings, the contradiction of expectations, quality, value, PLS.

Análisis de los factores de influencia en la adopción de herramientas colaborativas basadas en software social. Aplicación a entornos empresariales

En las últimas dos décadas, se ha puesto de relieve la importancia de los procesos de adquisición y difusión del conocimiento dentro de las empresas, y por consiguiente el estudio de estos procesos y la implementación de tecnologías que los faciliten ha sido un tema que ha despertado un creciente interés en la comunidad científica. Con el fin de facilitar y optimizar la adquisición y la difusión del conocimiento, las organizaciones jerárquicas han evolucionado hacia una configuración más plana, con estructuras en red que resulten más ágiles, disminuyendo la dependencia de una autoridad centralizada, y constituyendo organizaciones orientadas a trabajar en equipo. Al mismo tiempo, se ha producido un rápido desarrollo de las herramientas de colaboración Web 2.0, tales como blogs y wikis. Estas herramientas de colaboración se caracterizan por una importante componente social, y pueden alcanzar todo su potencial cuando se despliegan en las estructuras organizacionales planas. La Web 2.0 aparece como un concepto enfrentado al conjunto de tecnologías que existían a finales de los 90s basadas en sitios web, y se basa en la participación de los propios usuarios. Empresas del Fortune 500 –HP, IBM, Xerox, Cisco– las adoptan de inmediato, aunque no hay unanimidad sobre su utilidad real ni sobre cómo medirla. Esto se debe en parte a que no se entienden bien los factores que llevan a los empleados a adoptarlas, lo que ha llevado a fracasos en la implantación debido a la existencia de algunas barreras. Dada esta situación, y ante las ventajas teóricas que tienen estas herramientas de colaboración Web 2.0 para las empresas, los directivos de éstas y la comunidad científica muestran un interés creciente en conocer la respuesta a la pregunta: ¿cuáles son los factores que contribuyen a que los empleados de las empresas adopten estas herramientas Web 2.0 para colaborar? La respuesta a esta pregunta es compleja ya que se trata de herramientas relativamente nuevas en el contexto empresarial mediante las cuales se puede llevar a cabo la gestión del conocimiento en lugar del manejo de la información. El planteamiento que se ha llevado a cabo en este trabajo para dar respuesta a esta pregunta es la aplicación de los modelos de adopción tecnológica, que se basan en las percepciones de los individuos sobre diferentes aspectos relacionados con el uso de la tecnología. Bajo este enfoque, este trabajo tiene como objetivo principal el estudio de los factores que influyen en la adopción de blogs y wikis en empresas, mediante un modelo predictivo, teórico y unificado, de adopción tecnológica, con un planteamiento holístico a partir de la literatura de los modelos de adopción tecnológica y de las particularidades que presentan las herramientas bajo estudio y en el contexto especifico. Este modelo teórico permitirá determinar aquellos factores que predicen la intención de uso de las herramientas y el uso real de las mismas. El trabajo de investigación científica se estructura en cinco partes: introducción al tema de investigación, desarrollo del marco teórico, diseño del trabajo de investigación, análisis empírico, y elaboración de conclusiones. Desde el punto de vista de la estructura de la memoria de la tesis, las cinco partes mencionadas se desarrollan de forma secuencial a lo largo de siete capítulos, correspondiendo la primera parte al capítulo 1, la segunda a los capítulos 2 y 3, la tercera parte a los capítulos 4 y 5, la cuarta parte al capítulo 6, y la quinta y última parte al capítulo 7. El contenido del capítulo 1 se centra en el planteamiento del problema de investigación así como en los objetivos, principal y secundarios, que se pretenden cumplir a lo largo del trabajo. Así mismo, se expondrá el concepto de colaboración y su encaje con las herramientas colaborativas Web 2.0 que se plantean en la investigación y una introducción a los modelos de adopción tecnológica. A continuación se expone la justificación de la investigación, los objetivos de la misma y el plan de trabajo para su elaboración. Una vez introducido el tema de investigación, en el capítulo 2 se lleva a cabo una revisión de la evolución de los principales modelos de adopción tecnológica existentes (IDT, TRA, SCT, TPB, DTPB, C-TAM-TPB, UTAUT, UTAUT2), dando cuenta de sus fundamentos y factores empleados. Sobre la base de los modelos de adopción tecnológica expuestos en el capítulo 2, en el capítulo 3 se estudian los factores que se han expuesto en el capítulo 2 pero adaptados al contexto de las herramientas colaborativas Web 2.0. Con el fin de facilitar la comprensión del modelo final, los factores se agrupan en cuatro tipos: tecnológicos, de control, socio-normativos y otros específicos de las herramientas colaborativas. En el capítulo 4 se lleva a cabo la relación de los factores que son más apropiados para estudiar la adopción de las herramientas colaborativas y se define un modelo que especifica las relaciones entre los diferentes factores. Estas relaciones finalmente se convertirán en hipótesis de trabajo, y que habrá que contrastar mediante el estudio empírico. A lo largo del capítulo 5 se especifican las características del trabajo empírico que se lleva a cabo para contrastar las hipótesis que se habían enunciado en el capítulo 4. La naturaleza de la investigación es de carácter social, de tipo exploratorio, y se basa en un estudio empírico cuantitativo cuyo análisis se llevará a cabo mediante técnicas de análisis multivariante. En este capítulo se describe la construcción de las escalas del instrumento de medida, la metodología de recogida de datos, y posteriormente se presenta un análisis detallado de la población muestral, así como la comprobación de la existencia o no del sesgo atribuible al método de medida, lo que se denomina sesgo de método común (en inglés, Common Method Bias). El contenido del capítulo 6 corresponde al análisis de resultados, aunque previamente se expone la técnica estadística empleada, PLS-SEM, como herramienta de análisis multivariante con capacidad de análisis predictivo, así como la metodología empleada para validar el modelo de medida y el modelo estructural, los requisitos que debe cumplir la muestra, y los umbrales de los parámetros considerados. En la segunda parte del capítulo 6 se lleva a cabo el análisis empírico de los datos correspondientes a las dos muestras, una para blogs y otra para wikis, con el fin de validar las hipótesis de investigación planteadas en el capítulo 4. Finalmente, en el capítulo 7 se revisa el grado de cumplimiento de los objetivos planteados en el capítulo 1 y se presentan las contribuciones teóricas, metodológicas y prácticas derivadas del trabajo realizado. A continuación se exponen las conclusiones generales y detalladas por cada grupo de factores, así como las recomendaciones prácticas que se pueden extraer para orientar la implantación de estas herramientas en situaciones reales. Como parte final del capítulo se incluyen las limitaciones del estudio y se sugiere una serie de posibles líneas de trabajo futuras de interés, junto con los resultados de investigación parciales que se han obtenido durante el tiempo que ha durado la investigación. ABSTRACT In the last two decades, the relevance of knowledge acquisition and dissemination processes has been highlighted and consequently, the study of these processes and the implementation of the technologies that make them possible has generated growing interest in the scientific community. In order to ease and optimize knowledge acquisition and dissemination, hierarchical organizations have evolved to a more horizontal configuration with more agile net structures, decreasing the dependence of a centralized authority, and building team-working oriented organizations. At the same time, Web 2.0 collaboration tools such as blogs and wikis have quickly developed. These collaboration tools are characterized by a strong social component and can reach their full potential when they are deployed in horizontal organization structures. Web 2.0, based on user participation, arises as a concept to challenge the existing technologies of the 90’s which were based on websites. Fortune 500 companies – HP, IBM, Xerox, Cisco- adopted the concept immediately even though there was no unanimity about its real usefulness or how it could be measured. This is partly due to the fact that the factors that make the drivers for employees to adopt these tools are not properly understood, consequently leading to implementation failure due to the existence of certain barriers. Given this situation, and faced with theoretical advantages that these Web 2.0 collaboration tools seem to have for companies, managers and the scientific community are showing an increasing interest in answering the following question: Which factors contribute to the decision of the employees of a company to adopt the Web 2.0 tools for collaborative purposes? The answer is complex since these tools are relatively new in business environments. These tools allow us to move from an information Management approach to Knowledge Management. In order to answer this question, the chosen approach involves the application of technology adoption models, all of them based on the individual’s perception of the different aspects related to technology usage. From this perspective, this thesis’ main objective is to study the factors influencing the adoption of blogs and wikis in a company. This is done by using a unified and theoretical predictive model of technological adoption with a holistic approach that is based on literature of technological adoption models and the particularities that these tools presented under study and in a specific context. This theoretical model will allow us to determine the factors that predict the intended use of these tools and their real usage. The scientific research is structured in five parts: Introduction to the research subject, development of the theoretical framework, research work design, empirical analysis and drawing the final conclusions. This thesis develops the five aforementioned parts sequentially thorough seven chapters; part one (chapter one), part two (chapters two and three), part three (chapters four and five), parte four (chapters six) and finally part five (chapter seven). The first chapter is focused on the research problem statement and the objectives of the thesis, intended to be reached during the project. Likewise, the concept of collaboration and its link with the Web 2.0 collaborative tools is discussed as well as an introduction to the technology adoption models. Finally we explain the planning to carry out the research and get the proposed results. After introducing the research topic, the second chapter carries out a review of the evolution of the main existing technology adoption models (IDT, TRA, SCT, TPB, DTPB, C-TAM-TPB, UTAUT, UTAUT2), highlighting its foundations and factors used. Based on technology adoption models set out in chapter 2, the third chapter deals with the factors which have been discussed previously in chapter 2, but adapted to the context of Web 2.0 collaborative tools under study, blogs and wikis. In order to better understand the final model, the factors are grouped into four types: technological factors, control factors, social-normative factors and other specific factors related to the collaborative tools. The first part of chapter 4 covers the analysis of the factors which are more relevant to study the adoption of collaborative tools, and the second part proceeds with the theoretical model which specifies the relationship between the different factors taken into consideration. These relationships will become specific hypotheses that will be tested by the empirical study. Throughout chapter 5 we cover the characteristics of the empirical study used to test the research hypotheses which were set out in chapter 4. The nature of research is social, exploratory, and it is based on a quantitative empirical study whose analysis is carried out using multivariate analysis techniques. The second part of this chapter includes the description of the scales of the measuring instrument; the methodology for data gathering, the detailed analysis of the sample, and finally the existence of bias attributable to the measurement method, the "Bias Common Method" is checked. The first part of chapter 6 corresponds to the analysis of results. The statistical technique employed (PLS-SEM) is previously explained as a tool of multivariate analysis, capable of carrying out predictive analysis, and as the appropriate methodology used to validate the model in a two-stages analysis, the measurement model and the structural model. Futhermore, it is necessary to check the requirements to be met by the sample and the thresholds of the parameters taken into account. In the second part of chapter 6 an empirical analysis of the data is performed for the two samples, one for blogs and the other for wikis, in order to validate the research hypothesis proposed in chapter 4. Finally, in chapter 7 the fulfillment level of the objectives raised in chapter 1 is reviewed and the theoretical, methodological and practical conclusions derived from the results of the study are presented. Next, we cover the general conclusions, detailing for each group of factors including practical recommendations that can be drawn to guide implementation of these tools in real situations in companies. As a final part of the chapter the limitations of the study are included and a number of potential future researches suggested, along with research partial results which have been obtained thorough the research.

Nitric oxide in the contractile action of bradykinin, oxytocin, and prostaglandin F2 α in the estrogenized rat uterus

Experiments were performed on uteri from estrogen-primed female rats. Bradykinin (BK) (10−8 M) significantly augmented biosynthesis of prostaglandin F2 α (PGF2α) and prostaglandin E2 (PGE2), and this synthesis was completely blocked by NG-monomethyl l-arginine (NMMA) (300 μM), a competitive inhibitor of nitric oxide synthase (NOS). Blockade of prostaglandin synthesis by indomethacin caused rapid dissipation of isometric developed tension (IDT) induced by BK. Blockade of NOS with NMMA had similar but less marked effects. Combining the two inhibitors produced an even more rapid decay in IDT, suggesting that BK-induced NO release maintains IDT by release of prostanoids. The decline of frequency of contraction (FC) was not significantly altered by either indomethacin or NMMA but was markedly accelerated by combination of the inhibitors, which suggests that PGs maintain FC and therefore FC decline is accelerated only when PG production is blocked completely by combination of the two inhibitors of PG synthesis. The increase in IDT induced by oxytocin was unaltered by indomethacin, NMMA or their combination indicating that neither NO nor PGs are involved in the contractions induced by oxytocin. However, the decline in FC with time was significantly reduced by the inhibitor of NOS, NMMA, suggesting that FC decay following oxytocin is caused by NO released by the contractile process. In the case of PGF2α, NMMA resulted in increased initial IDT and FC. The decline in FC was rapid and dramatically inhibited by NMMA. Receptor-mediated contraction by BK, oxytocin, and PGF2α is modulated by NO that maintains IDT by releasing PGs but reduces IDT and FC via cyclic GMP.

Manual del farmacéutico ó Compendio elemental de farmacia... /

Mode of access: Internet.

Manuel du pharmacien ou Précis élémentaire de pharmacie /

Mode of access: Internet.

Energy-based damage and fracture framework for viscoelastic asphalt concrete

A framework based on the continuum damage mechanics and thermodynamics of irreversible processes using internal state variables is used to characterize the distributed damage in viscoelastic asphalt materials in the form of micro-crack initiation and accumulation. At low temperatures and high deformation rates, micro-cracking is considered as the source of nonlinearity and thus the cause of deviation from linear viscoelastic response. Using a non-associated damage evolution law, the proposed model shows the ability to describe the temperature-dependent processes of micro-crack initiation, evolution and macro-crack formation with good comparison to the material response in the Superpave indirect tensile (IDT) strength test.

Analysing intention and action in mobile banking services

Technological advances at the level of mobile devices are transforming the world. Banking users are able to conduct banking services at any place, and at any time, with m-banking. The purpose of this research is to analyse intention and action in m-banking services. A research model was developed and PLS was used to test the causalities in the proposed model. Our proposal extends the existing models with an assessment of the actual usage of m-banking and of how behavioural intention translates into action. This study found that the main determinants of behavioural intention for m-banking are social influence and relative advantage. Furthermore, perceived risk, lack of information and usage barriers have a negative effect on m- banking behavioural intention. Perceived risks, e-banking use, and behaviour intention are found to be significant antecedents of m-banking use. Gender has a positive and significant influence on m-banking usage, but not on the construction of behavioural intention.

Allergological evaluation of a dog population in a veterinary immuno-allergology consultation: What correlates in a canine model

Introduction: Allergic dermatitis (AD) is the most common canine pruritic condition in veterinary dermatology. Allergic dermatitis to flea bites presents the highest prevalence, followed by atopic dermatitis and food AD. This study aimed to identify possible correlation between data from clinical signs, intradermal tests (IDT) and specific IgE levels, which are used in dog AD assessment. Methods: Fifty five dogs from the Veterinary Hospital of the University of Évora (Portugal) and Rof Codina University Hospital (Lugo, Spain) outpatient consultations were studied by means of clinical inquiry, IDT and specific IgE determination. Thirty five of the patients belonged to predisposed breeds, 30 were females and 25 males. Forty one (74%) were indoor. Results: In 82% of cases first clinical signs appeared before the age of 3 years and 24% even before 1 year old. In 70% of the individuals clinical signs included itching, which was generalized in 66%, with 78% of paw licking and chewing. Clinical profile showed seasonal worsening in 64% of cases. From the 69.1% of dogs already presenting with dermatitis, 50% also presented external otitis and 28.9% self-inflicted alopecia. "Intense itching" was found in 10.5%, "medium itching" in 81.6% and “mild itching” in 5.26% of the patients. Prevalence of positive IDT was 37.3 % to Lep d, 29.41% to Der f, 27.5% to Der p, 25.5% to Dac g and 21.6% to Malassezia sp. From the 37 dogs submitted to food IDT, 40.5% revealed positive to beef, 27% to chicken, 27% to porc and 5.4% to lamb. Specific IgE > 150 EAU was found in 84% of dogs to indoor allergen sources and in 68% to pollens. A negative correlation was found between an outdoor life and the intensity (p = 0.033) and precocity (p = 0.026) of clinical signs. Sensitization to pollens was found positively correlated with the seasonality of clinical signs (p = 0.001) and the positivity for Dac g (p = 0.007). The prevalence of chronic otitis correlated positively with alopecia and reactivity to Lep d (p = 0.008), Plantago lanceolata (p = 0.026) and Platanus acerifolia (p = 0.017). There was no correlation between the results of ITD and specific IgE. Conclusion: We can conclude that correlation between different clinical signs and positive testing for some allergenic sources may occur, as well as between sensitization to pollens and the beginning, the intensity and the seasonality of dog patient clinical signs.

Synthesis of new non-fullerene acceptors based on indacenodithiophene core for organic solar cells

Due to the low cost, lightness and flexibility, Polymer Solar Cell (PSC) technology is considered one of the most promising energy technologies. In the past decades, PSCs using fullerenes or fullerene derivatives as the electron acceptors have made great progress with best power conversion efficiency (PCE) reaching 11%. However, fullerene type electron acceptors have several drawbacks such as complicated synthesis, a low light absorption coefficient and poor tuning in energy levels, which prevent the further development of fullerene-based PSCs. Hence the need to have a new class of electron acceptors as an alternative to conventional fullerene compounds. Non-fullerene acceptors (NFAs) have developed rapidly in the last years and the maximum PCEs have exceeded 14% for single-junction cells and 17% for double-junction tandem cells. By combining an electron-donating backbone, generally with several fused rings with electron-withdrawing units, we can simply construct NFA of the acceptor–donor–acceptor type (A–D–A). Versatile molecular structures have been developed using methods such as acceptor motif engineering and donor motif engineering. However, there are only a few electron-donating backbones that have been proved to be successful. Therefore, it is still necessary to develop promising building blocks to further enrich the structural diversity. An indacenodithiophene (IDT) unit with just five fused rings has a sufficiently rigid coplanar structure, which has been regarded as one of the promising electron-rich units to design high-performance A–D–A NFAs. In this work, performed at the King Abdullah University of Science and Technology in Saudi Arabia, a new nine-cyclic building block (TBIDT) with a two benzothiophene unit was synthesized and used for designing new non-fullerene electron acceptors.