A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

Consumo no Brasil : quebras estruturais e suavização do consumo

A teoria de consumo indica que os indivíduos maximizam sua utilidade se suavizarem o consumo ao longo da vida. Manter o consumo constante seria melhor que se sujeitar à instabilidade. Entretanto a maior parte dos testes realizados com informações brasileiras contraria esta hipótese. Este trabalho apresenta resultados diferentes, com indícios de suavização do consumo em um período recente. Para a realização deste trabalho, foram utilizados dados nacionais extraídos do site do IPEA (IPEADATA), onde estão disponíveis séries de consumo, renda, juros, crédito, assim como deflatores. As séries foram tratadas de maneira a se padronizarem como trimestrais, incorporando o período entre primeiro trimestre de 1991 e o segundo trimestre de 2009. Com o auxílio de técnica de estimação de quebras estruturais, ficaram evidentes os momentos em que as séries macroeconômicas tiveram mudanças significativas. Desta maneira, o histórico de informações foi subdividido conforme o panorama econômico. Cada período deste histórico foi submetido a um processo de estimação de crescimento de consumo a partir de crescimento de renda, juros e crescimento de crédito. A teoria de suavização do consumo sugere que a estimação deveria resultar em estimativas não significantes, pois o consumo não estaria atrelado ao acesso ao credito ou variações temporárias da renda. O que se verificou foi que em um histórico mais distante, os resultados foram bastante parecidos com resultados observados em bibliografia. Entretanto, em um período mais recente o consumo estaria se desvencilhando da renda e do crédito. Isto sugere que a suavização do consumo pode estar se concretizando no Brasil.

International Recommendations for Training Future Toxicologic Pathologists Participating in Regulatory-Type, Nonclinical Toxicity Studies

The International Federation of Societies of Toxicologic Pathologists (IFSTP) proposes a common global framework for training future toxicologic pathologists who will support regulatory-type nonclinical toxicology studies. Trainees optimally should undertake a scientific curriculum of at least 5 years at an accredited institution leading to a clinical degree (veterinary medicine or medicine). Trainees should then obtain 4 or more years of intensive pathology practice during a residency and/or on-the-job "apprenticeship," at least 2 years of which must be focused on regulatory-type toxicologic pathology topics. Possession of a recognized pathology qualification (i.e., certification) is highly recommended. A non-clinical pathway (e.g., a graduate degree in medical biology or pathology) may be possible if medically trained pathologists are scarce, but this option is not optimal. Regular, lifelong continuing education (peer review of nonclinical studies, professional meetings, reading, short courses) will be necessary to maintain and enhance one's understanding of current toxicologic pathology knowledge, skills, and tools. This framework should provide a rigorous yet flexible way to reliably train future toxicologic pathologists to generate, interpret, integrate, and communicate data in regulatory-type, nonclinical toxicology studies. (J Toxicol Pathol 2010; 23: 171-181)

International recommendations for training future toxicologic pathologists participating in regulatory-type, nonclinical toxicity studies

The International Federation of Societies of Toxicologic Pathologists (IFSTP) proposes a common global framework for training future toxicologic pathologists who will support regulatory-type - nonclinical toxicology studies. Trainees optimally should undertake a scientific curriculum of at least 5 years at an accredited institution leading to a clinical degree (veterinary medicine or medicine). Trainees should then obtain 4 or more years of intensive pathology practice during a residency and/or on-the-job "apprenticeship," at least 2 years of which must be focused on regulatory-type toxicologic pathology topics. Possession of a recognized pathology qualification (i.e., certification) is highly recommended. A nonclinical pathway (e.g., a graduate degree in medical biology or pathology) may be possible if medically trained pathologists are scarce, but this option is not optimal. Regular, lifelong continuing education (peer review of nonclinical studies, professional meetings, reading, short courses) will be necessary to maintain and enhance one's understanding of current toxicologic pathology knowledge, skills, and tools. This framework should provide a rigorous yet flexible way to reliably train future toxicologic pathologists to generate, interpret, integrate, and communicate data in regulatory-type, nonclinical toxicology studies. (C) 2010 Reprinted by Permission of SAGE Publications Inc. Published by Elsevier GmbH. All rights reserved.

[Estimates from Walton & Moore for the raising and altering of seats in the anatomical room, and for repairing the lower room, northwest corner of the College House], 2 December 1806

The estimate includes projected costs for the materials and labor required for each renovation. The anatomical room needed its circular seats raised and altered. The renovation of the lower room included making new window frames and sashes (including replacing glass), installing new doors, and repairing the floors.

University of Michigan Men's Basketball Team, 1962

Back row: coach Dave Strack, Charles Higgs, James Ludwig, Douglas Greenwald, Dana Baldwin, Thomas Cole, John Harris, John Oosterbaan, David Kroll, asst. coach James Skala, asst. coach Tom Jorgenson

Front Row: Hiram Jackson, Thomas Withrow, Thomas Eveland, captain Jon Hall, Robert Cantrell, Douglas Herner, Steven Schoenherr, Barry Andrews

Haitian Creole: grammar, texts, vocabulary,

"Also appears as Memoir no. 74 of the American Anthropological Association."

Melanesian pidgin phrase-book and vocabulary with grammatical introduction.

Mode of access: Internet.

An authentic narrative of the proceedings of the expedition under the command of Brigadier-Gen. Craufurd, until its arrival at Monte Video; with an account of the operations against Buenos Aires under the command of Lieut.-Gen. Whitelocke.

Mode of access: Internet.

Die russischen Militärcolonien : ihre Einrichtung, Verwaltung und gegenwärtige Beschaffenheit ; aus dem Englischen.

Tr. of: An account of the military colonies in Russia.

Strictures and remarks on the Earl of Selkirk's Observations on the present state of the Highlands of Scotland, with a view of the causes and probable consequences of emigration.

Bound with: Amicus (pseud.) Eight letters on the subject of the Earl of Selkirk's pamphlet on Highland emigration. 1806.

The living age ...

Editors: 1844-70, E. Littell; 1870-96, R.S. Littell.

Lexicon graecum in tragicos; vel, Collectio interpretationum veterum ...

Cover-title.

The missing fragment of the Latin translation of the Fourth Book of Ezra /

Mode of access: Internet.

The life of Harman Blennerhassett. Comprising an authentic narrative of the Burr expedition: and containing many additional facts not heretofore published.

Mode of access: Internet.