Avaliação do potencial de utilização de percolado bruto e tratado fotoeletroquimicamente de aterro sanitário no desenvolvimento da espécie Helianthus annuus L

Os processos de decomposição dos resíduos sólidos resultam em uma importante fonte de poluente tóxico, o percolado, usualmente denominado de chorume, podendo causar graves impactos ambientais em ecossistemas vizinhos, se não for drenado e tratado adequadamente. No tratamento eletrolítico de efluentes aquosos, os poluentes sofrem degradação oxidativa. Visando o tratamento do percolado de aterro sanitário, foram analisados os parâmetros de pH, temperatura, DQO, condutividade, amônia e absorbância, utilizando-se de uma densidade de corrente de 125 mA.cm-2. As regas na espécie Helianthus annuus L., girassol da família Asteraceae, foram realizadas durante 95 dias com 11 tratamentos do percolado bruto e tratado em concentrações de 25, 50, 75 e 100% de percolado com a adição de Na2SO4, esta última concentração também sem adição de Na2SO4, a fim de avaliar a sobrevivência e o crescimento dessa espécie. Ao final do experimento, os valores de crescimento mais expressivos foram para o percolado 100% sem Na2SO4 mostrando que: a altura total obteve uma diferença em 27% menor no tratado; o diâmetro da base do caule mostrou uma diferença 45% menor do tratado em relação ao bruto; o número de folhas foi de 65% menor e área foliar de 51% menor do tratado em relação ao bruto, indicando que mesmo em crescimento o Grupo Tratado permaneceu menor durante a maioria das medidas das variáveis biométricas do girassol BRS 323. Todos os vasos regados com chorume tratado tiveram uma redução no peso seco das partes aéreas, chegando a uma redução média de 72% na concentração 100% de chorume sem a adição de Na2SO4

Anregung zur Cultur der Sonnenrose, Helianthus annuus L., und deren Nutzen

Von Grunert

Influence of light on DNA content of Helianthus annuus Linnaeus.

Mean nuclear 2C DNA content (C equaling haploid DNA per nucleus) of the first leaf of the sunflower, Helianthus annuus L., is influenced by the quality and the quantity of light. Seedlings of two inbred lines, RHA 299 and RHA 271 were germinated and grown in controlled environmental conditions. Lighting was adjusted to provide different combinations of photon flux densities and red to far red (R:FR) ratios. At R:FR = 5.8 and photon flux densities of 170 mumol.m-2.s-1, 200 mumol.m-2.s-1, and 230 mumol.m-2.s-1, DNA content remained high and relatively constant (x = 6.97 pg for RHA 271 and x = 7.32 pg for RHA 299). When the photon flux density range (R:FR = 5.8) was elevated to 350 mumol.m-2.s-1, 410 mumol.m-2.s-1, and 470 mumol.m-2.s-1, mean DNA content was reduced to 6.23 pg (RHA 271) and 6.46 pg (RHA 299). At R:FR = 1.5, mean DNA content was consistently high (7.2-7.9 pg) only at the lowest photon flux density of 170 mumol.m-2.s-1. Significant decreases in DNA content (< or = 12%) were observed at photon flux densities of 200 mumol.m-2.s-1 and 230 mumol.m-2.s-1. At the higher photon flux densities (350 mumol.m-2.s-1, 410 mumol.m-2.s-1, and 470 mumol.m-2.s-1) and R:RF = 1.5, the plants had extremely low DNA contents (mean x = 3.36 pg for RHA 271 and 3.41 pg for RHA 299) and high between-plant variance. The instability of DNA content, particularly for plants grown under light that is far red rich, suggests that phytochromes may be involved in regulating DNA content of the sunflower.

Influência do sistema anteoxidante da catalase no ciclo do glioxilato durante o estabelecimento pós-germinativo de cártamo (Carthamus tinctorius L.) e de girassol (Helianthus annuus L.)

Oilseeds are a high-value natural resource, due to its use as a substitute for petroleum. However, the storage time can reduce seed viability and oil quality. Therefore, scientific efforts have been made to provide a increment of storage time, germination rates and plant establishment of high-value oilseeds. The seedling establishment depends of the plant pass over the functional transition stage, characterized by a metabolic change from heterotrophic condition to autotrophic one. The storage oil mobilization is performed by β-oxidation process and the glyoxylate cycle. Also, the functional transition involves acclimation to photosynthetic condition, which generally includes the participation of antioxidant system and the reactive oxygen species, the latter are produced in various reactions of primary and secondary metabolism. In the present study, Catalase was inhibited during the functional transition of sunflower and safflower, after were performed many analyzes to elucidate the effects caused on the SOD and APX antioxidant systems. Also, were checked the changes in expression pattern of the glyoxylate cycle enzymes markers, ICL and MLS. It was observed that after CAT inhibition, the SOD and APX antioxidant systems allow the seedling establishment. Besides, was verified that both oilseeds can be accelerate the reverse mobilization and the photosynthetic establishment when Catalase activity has dramatically decreased

Flavonoids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication

Flavonoids in Australian honeys from five botanical species (Melaleuca, Guioa, Lophostemon, Banksia and Helianthus) have been analyzed in relation to their floral origins. Tea tree (Melaleuca quinquenervia) and heath (Banksia ericifolia) honeys show a common flavonoid profile comprising myricetin (3,5,7,3',4',5'-hexahydroxyflavone), tricetin (5,7,3',4,5'-pentahydroxyflavone), querectin (3,5,7,3',4'-pentahydroxyflavone) and luteolin (5,7,3',4'-tetrahydroxyflavone), which was previously suggested as a floral marker for an Australian Eucalyptus honey (bloodwood or Eucalyptus intermedia honey). These honeys of various floral species can be differentiated by their levels of total flavonoids, being 2.12 mg/100 g for heath honey and 6.35 m/100 g for tea tree honey. In brush box (Lophostemon conferta) honey, the flavonoid profile comprising mainly tricetin, luteolin and quercetin is similar to that of another Eucalyptus honey (yellow box or Eucalyptus melliodora honey). These results indicate that the flavonoid profiles in some of the Australian non-Eucalyptus honeys may contain more or less certain flavonoids from Eucalyptus floral sources because of the diversity and extensive availability of Eucalyptus nectars for honeybee foraging yearly around or a possible cross contamination of the monofloral honeys during collection, transportation and/or storage. Further analyses are required to differentiate and/or verify the botanical sources of the flavonoids that contribute to the flavonoid profiles of these honeys, by restricting honey sampling areas and procedures, employing other complementary analytical methods (e.g. pollen analysis, sugar profile) and using materials (e.g. nectar) directly sourced from the flowering plant for comparative studies. In Australian crow ash (Guioa semiglauca) honey, myricetin, tricetin, quercetin, luteolin and an unknown flavonoid have been found to be the main flavonoids, which is characteristic only to this type of honey, and could thus be used as the floral marker, while in Australian sunflower (Helianthus annuus) honey, the content of total flavonoids is the smallest amount comparing to those in the other honeys analysed in this study. However, the flavonoid quercetin and the flavonoid profile mainly consisting of quercetin, quercetin 3,3'-dimethyl ether (5,7,4'-trihydroxy3,3'-dimethoxyflavone), myricetin and luteolin are characteristic only to this sunflower honey and could thus be used for the authentication.

Phenolic acids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication

Eight phenolic acids and two abscisic acid isomers in Australian honeys from five botanical species (Melaleuca, Guioa, Lophostemon, Banksia and Helianthus) have been analyzed in relation to their botanical origins. Total phenolic acids present in these honeys range from 2.13 mg/100 g sunflower (Helianthus annuus) honey to 12.11 mg/100 g tea tree (Melaleuca quinquenervia) honey, with amounts of individual acids being various. Tea tree honey shows a phenolic profile of gallic, ellagic, chlorogenic and coumaric acids, which is similar to the phenolic profile of an Australian Eucalyptus honey (bloodwood or Eucalyptus intermedia honey). The main difference between tea tree and bloodwood honeys is the contribution of chlorogenic acid to their total phenolic profiles. In Australian crow ash (Guioa semiglauca) honey, a characteristic phenolic profile mainly consisting of gallic acid and abscisic acid could be used as the floral marker. In brush box (Lophostemon conferta) honey, the phenolic profile, comprising mainly gallic acid and ellagic acid, could be used to differentiate this honey not only from the other Australian non-Eucalyptus honeys but also from a Eucalyptus honey (yellow box or Eucalyptus melliodora honey). However, this Eucalyptus honey could not be differentiated from brush box honey based only on their flavonoid profiles. Similarly, the phenolic profile of heath (Banksia ericifolia) honey, comprising mainly gallic acid, an unknown phenolic acid (Phl) and coumaric acid, could also be used to differentiate this honey from tea tree and bloodwood honeys, which have similar flavonoid profiles. Coumaric acid is a principal phenolic acid in Australian sunflower honey and it could thus be used together with gallic acid for the authentication. These results show that the HPLC analysis of phenolic acids and abscisic acids in Australian floral honeys Could assist the differentiation and authentication of the honeys. © 2005 Elsevier Ltd. All rights reserved.

Austropleospora osteospermi gen. et sp nov and its host specificity and distribution on Chrysanthemoides monilifera ssp rotundata in Australia.

Hendersonia osteospermi was found for the first time in Australia on leaf spots of the introduced invasive plant Chrysanthemoides monilifera ssp. rotundata (bitou bush) in coastal regions of New South Wales. Pathogenicity tests on species from 11 tribes in the family Asteraceae, demonstrated that H. osteospermi caused severe necrosis on leaves and stems of C. monilifera ssp. rotundata and its congener C. monilifera ssp. monilifera (boneseed). Small necrotic spots also developed on Osteospermum fruticosum and Dimorphotheca cuneata in the Calenduleae and on Helianthus annuus (sunflower) in the Heliantheae. None of the other plant species tested developed leaf spots, although H. osteospermi was re-isolated from senescent leaves of Cynara scolymus (globe artichoke) in the Cynareae and Vernonia cinerea in the Vernonieae. Single ascospores from ascomata of a Pleospora-like fungus found on diseased stems of bitou bush produced H. osteospermi in culture, which proved the anamorph/teleomorph connection. The ITS region of both a single-ascospore isolate and a single-conidium isolate were sequenced and found to be identical. The taxonomic status of H. osteospermi is re-examined and Austropleospora osteospermi gen. et sp. nov. is described as its teleomorph based on morphology, host range tests and DNA sequence analysis. The potential of A. osteospermi for the biological control of bitou bush and boneseed in Australia is discussed.

Plasmopara sphagneticolae sp. nov. (Peronosporales) on Sphagneticola (Asteraceae) in Australia

A specimen of downy mildew on leaves of Sphagneticola trilobata found in northern Queensland was identified by a systematic approach as a novel species of Plasmopara. A new species, Plasmopara sphagneticolae, is proposed for this specimen, which differs from other species of Plasmopara by morphology, host range, and sequence data from nuclear-ribosomal DNA and mitochondrial DNA. Plasmopara sphagneticolae, together with P. halstedii, are downy mildews found on host species in the tribe Heliantheae (Asteraceae). Plasmopara halstedii causes downy mildew on Helianthus annuus, and is not present on sunflower in Australia. Phylogenetic analysis of the large subunit region of ribosomal DNA showed that P. sphagneticolae was sister to P. halstedii on sunflower.

Viability of seeds in food products proposed for field trips in Galapagos

Seeds of 14 plant species extracted from food products proposed for field trips in Galapagos were tested for viability. Strawberry Fragaria ananasa and Blackberry Rubus glaucus jams (Snob and Gustadina brands) contained no viable seeds. Schullo brand granola contained inviable Sesame Sesamum indicum seed, but Sesame in granolas prepared in Galapagos was viable. Sesame seed in bread was viable but Flax Linum usitatissimum seed in bread was not. Brown Rice Oryza sativa and Sunflower seeds Helianthus annuus were both viable. Fresh Apple Malus domestica, Naranjilla Solanum quitoense, Cucumber Cucumis sativus, Pineapple Ananas comosus, Pear Pyrus communis, Bell Pepper Capsicum annuum, Tomato Solanum lycopersicum, Grape Vinis vinifera all contained viable seeds. We recommend prohibiting any product with viable seeds from field trips to uninhabited areas. CDF Contribution Number 1009.

镉、铅污染土壤的植物-微生物联合修复研究

植物－微生物联合修复是利用微生物作为植物修复重金属土壤的一种强化手段，在弥补单纯植物及微生物修复技术不足的同时，利用植物和微生物的共存体系提高植物修复效率。本研究考察了向日葵（Helianthus annuus L.），芥菜（Brassica juncea L.），紫花苜蓿（Medicago sativa L.）和蓖麻（Ricinus communis L.）对Cd、Pb的富集特征，并筛选出向日葵作为富集植物；探讨了向日葵根系分泌物在重金属胁迫下的变化；通过重金属耐受菌株与向日葵的配伍对Cd、Pb污染土壤进行联合修复，结果如下： 液体培养实验结果表明，四种植物对Cd、Pb富集能力明显不同，其中向日葵对两种重金属的提取效果较好。四种植物对重金属的富集量随着浓度的增加而增加，而富集系数随重金属浓度的增加而减小，转移系数同重金属浓度及地上部/地下部生物量比值呈现一定的相关性；Cd、Pb复合处理中，一种重金属的存在会在不同程度上影响植物对另一种重金属的吸收；此外，不同植物及重金属处理中根际区域的酸碱度及氧化还原电位呈现负相关性。 砂培实验结果表明，向日葵对重金属的富集规律基本同液体培养实验相似。富集系数与重金属浓度和培养时间呈现线性相关关系。复合处理中，当Cd和Pb在适当浓度比例时，向日葵可以增加对某一重金属的吸收效率。向日葵的根系分泌物组成因重金属的存在而明显减少，根系分泌物中的草酸、酒石酸、苹果酸、柠檬酸、乙酸及丁二酸含量随着不同浓度Cd、Pb而发生不规则变化。 在以根系分泌物作为唯一营养来源筛选重金属耐受菌株实验中，细菌和真菌对Cd及Pb的耐性及吸附效率不同。总体上看，微生物生物量随重金属浓度升高而降低，而重金属吸附量随浓度升高而增加，重金属复合毒性也减少了微生物对单一重金属的吸收；另外，培养基中酸碱度因微生物种类及重金属浓度而有所差异。 将筛选出的优势微生物与向日葵配伍处理Cd、Pb污染土壤的实验中，由于Cd、Pb污染模式及菌株种类的不同，微生物对向日葵吸收重金属的强化效果呈现很大差异，其中真菌对植物吸收重金属的强化能力较细菌强；同时，根系分泌物中6种有机酸的含量在不同处理中变化较大；在处理单一Cd或Pb污染的时候选用菌株B1、F1或混合菌B1+B2、F1+F2、B2+F1、B1+F2与向日葵配伍修复的效果较好；混合菌F1+F2、B1+F2、B2+F1的添加能较好的强化Cd、Pb复合污染中向日葵吸收重金属的能力。

Resultados de pesquisa da Embrapa Soja - 2000: girassol e trigo.

Tecnologias para o desenvolvimento da cultura do girassol no Brasil; Desenvolvimento de germoplasma e de cultivares de girassol (04.0.99.334-01); Rede de ensaios de avaliação de genótipos de girassol (04.0.99.334-02); Avaliação de danos da mancha de alternaria em girassol (04.0.99.334-03); Caracterização da aptidão climática de regiões para o cultivo do girassol (04.0.99.334-04); Avaliação de herbicidas para a cultura do girassol (04.0.99.334-05); Validação e difusão de tecnologias para a produção de girassol no Brasil (04.1999.334-07); Melhoramento genético de trigo para a Região Centro-sul brasileira; Desenvolvimento e avaliação de cultivares de trigo para o Estado do Paraná (04.1999.352-02).

Produtividade de genótipos de girassol em diferentes épocas de semeadura no oeste da Bahia.

2009