Fast and efficient synthesis of pyrano[3,2-c]quinolines catalyzed by niobium(V) chloride

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Triplex targeting of human PDGF-B (c-sis, proto-oncogene) promoter specifically inhibits factors binding and PDGF-B transcription

Human c-sis/PDGF-B proto-oncogene has been shown to be overexpressed in a large percentage of human tumor cells establishing a growth-promoting, autocrine growth circuit. Triplex forming oligonucleotides (TFOs) can recognize and bind sequences in duplex DNA, and have received considerable attention because of their potential for targeting specific genomic sites. The c-sis/PDGF-B promoter contains a unique homopurine/homopyrimidine sequence (SIS proximal element, SPE), which is crucial for binding nuclear factors that provoke transcription. In order to develop specific transcriptional inhibitors of the human c-sis/PDGF-B proto-oncogene, 20 potential TFOs targeting part or all of the SPE were screened by gel mobility analysis. DNase I footprinting shows that the TFOs we designed can form a sequence-specific triplex with the target. Protein binding assays demonstrate that triplex formation inhibits nuclear factors binding the c-sis/PDGF-B promoter. Both transient and stable transfection experiments demonstrate that the transcriptional activity of the promoter is considerably inhibited by the TFOs. We propose that TFOs represent a therapeutic potential to specifically diminish the expression of c-sis/PDGF-B proto-oncogene in various pathologic settings where constitutive expression of this gene has been observed.

The life of Sir Stanley Maude, lieutenant-general, K. C. B., C. M. G., D. S. O.

Mode of access: Internet.

The student's Roman empire. A history of the Roman empire from its foundation to the death of Marcus Aurelius [27 B.C.-180 A.D.]

Mode of access: Internet.

History of Switzerland, from B.C. 110, to A.D. 1830.

Includes index.

Forty years in the Turkish Empire, or, Memoirs of Rev. William Goodell : late missionary of the A.B.C.F.M. at Constaninople /

Mode of access: Internet.

Journal of an exploring tour beyond the Rocky Mountains, under the direction of the A.B.C.F.M. performed in the years 1835, '36, and '37;

Sabin 58729; Wagner 70.

Observations of comets : from 611 B.C. to A.D.1640 : extracted from the Chinese annals /

Reprint. Originally published: London : s.n., 1871.

Workforce Patterns and the Prevention of Mother-to-Child Transmission of HIV Option B Cascade in Côte d'Ivoire

Thesis (Master's)--University of Washington, 2016-08

Copy from actual survey of the 3 Sisters : [Potomac River, Washington D.C.] /

Cadastral map.

The Roman empire : B. C. 29-A. D. 476 /

Includes index.

Illustrated English history, B.C. 55-A.D.[1886]

Mode of access: Internet.

Alsace and Lorraine from Cæsar to Kaiser, 58 B.C.-1871 A.D.,

Appendices: I. The treaties; diplomatic articles by which the political status of Alsace and Lorraine ... has been affected (p. 201-205); II. Main sources used for the narrative (p. 206-208)