Sorting by diffusion: An asymmetric obstacle course for continuous molecular separation

A separation technique employing a microfabricated sieve has been demonstrated by observing the motion of DNA molecules of different size. The sieve consists of a two-dimensional lattice of obstacles whose asymmetric disposition rectifies the Brownian motion of molecules driven through the device, causing them to follow paths that depend on their diffusion coefficient. A nominal 6% resolution by length of DNA molecules in the size range 15–30 kbp may be achieved in a 4-inch (10-cm) silicon wafer. The advantage of this method is that samples can be loaded and sorted continuously, in contrast to the batch mode commonly used in gel electrophoresis.

Fixing human factor IX (fIX): correction of a cryptic RNA splice enables the production of biologically active fIX in the mammary gland of transgenic mice.

Transgenic mice and sheep secrete only low levels of human factor IX in their milk because of an aberrant splicing of the transgene RNA in the mammary gland. Removal of the cryptic 3' splice site prevents this splicing and leads to the production of relatively high levels of factor IX. The purified protein is fully active showing that the mammary gland is capable of the efficient post-translational modification of this protein and that transgenic animals are a suitable means of its production.

What causes collisions? /

"Released January 15, 1949."

Investigation of a radioactivity technique for the determination of dissolved oxygen /

Mode of access: Internet.

Flexible geiger counters /

"Task No. 4 ; Contract No. AT-(40-1)-2513"--Added title page.

Investigation of a tritium enrichment technique : final report /

"RTI-2513-T2-1"--Cover.

Investigation of a geiger counter technique for determining the position in space of individual ionizing particles /

"Contract No. AT-(40-1)-2513."

A radio-release technique for the measurement of moisture in gases : final report /

"Contract No. AT-(40-1)-2513."

A dissolved oxygen analyzer : final report /

"Other issues of this report may bear the number RTI-2513-T11"--Page 2 of cover.

The evaluation of complex educational outcomes /

Includes bibliographical references (p. 43-44).

Ueber die elektromotorische Kraft einiger geschmolzener Halogen-Schwermetallketten.

Thesis (doctoral)--Universitat Basel.

Pain,

Bibliography: p.99-112.

Improving language arts instruction in the elementary school

Mode of access: Internet.

Report of cases determined by the Supreme Court of Appeals of West Virginia.

Imprint varies.