ATENDIMENTO ONLINE POR CHAT: ADEQUANDO OS SERVIÇOS DE BIBLIOTECA A UM NOVO PÚBLICO

O trabalho relata a experiência da Biblioteca Virginie Buff D���Ápice da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ/USP) na implantação do sistema de atendimento online por chat e analisa estatística e qualitativamente os 527 atendimentos realizados entre Agosto de 2009 e Junho de 2010. Além disso, apresenta o impacto direto desse sistema na readequação do Serviço de Atendimento ao Usuário

Heparin modulation of laminin polymerization

Previously, it has been shown that laminin will self-assemble by a two-step calcium-dependent process using end-domain interactions (Yurchenco, P. D., Tsi-library, E. C., Charonis, A. S., and Furthmayr, H. (1985) J. Biol. Chem. 260, 7636-7644). We now find that heparin, at low concentrations, modifies this polymerization by driving the equilibrium further toward aggregation, by producing a denser polymer, and by inducing aggregation in the absence of calcium. This effect on self-assembly is specific in that it is observed with heparin but not with several heparan sulfates or other glycosaminoglycans: it correlates with affinity and depends on the degree of polysaccharide sulfation. Heparin binds to laminin in a calcium-dependent manner with a single class of interaction (KD = 118 +/- 18 nM) and with a binding capacity of one heparin for two laminins. We find the long arm globule (E3) is the only laminin domain which exhibits substantial heparin binding: heparin binds E3 with an affinity (KD = 94 +/- 12 nM) and calcium dependence similar to that for intact laminin. These data strongly suggest that heparin modifies laminin assembly by binding to pairs of long arm globular domains. As a result the polymer may be stabilized at domain E3 and laminin interdomain interactions induced or modified. We further postulate that heparins may act in vivo as specific regulators of the structure and functions of basement membranes by both altering the laminin matrix and by displacing weakly binding heparan sulfates.

Illegal/undocumented aliens /

"Date originated 11/06/74; date updated 11/06/81."

Sendero Luminoso : a bibliography /

Cover title.

Sendero Luminoso : a bibliography /

Cover title.

Women come to the front : journalists, photographers, and broadcasters during World War II.

Shipping list no.: 96-0016-P.

Networks for Networkers II Conference /

"March, 1991."

Public affairs bulletins.

Each no. has also a distinctive title.

L'Année politique.

Title varies slightly.

Water rights and the control of waters : references supplementary to the printed list issued in 1914 /

"This list is confined to publications listed since 1914."

Presidents of the United States--their written measure : a bibliography /

Mode of access: Internet.

CIP publishers manual.

Mode of access: Internet.

CIP publishers manual.

Mode of access: Internet.

German-American relations : a selective bibliography /

Mode of access: Internet.

Music of the American Indian : Northwest (Puget Sound) : from the Archive of Folk Culture /

Mode of access: Internet.