Demersal fishery resources of Sri Lanka and its present level of exploitation with special reference to off-shore and deep-sea waters

Following a brief review of development of demersal fishing off Sri Lanka, the author reviews the fishing grounds of the Wadge Bank, the Pedro Bank, and the Mannar Bank. He reviews the deepwater trawling fisheries particularly in relation to the 1972 survey conducted by the 'Optimist' and also small boat trawling in coastal waters. Lastly he considers handlining for groundfish, which is principally conducted by fishermen operating traditional craft.

Pelagic fishery resources of Sri Lanka and its present level of exploitation with special reference to off-shore and deep-sea waters

Pelagic resources around Sri Lanka may be categorized into three major groups: (1) the small pelagic varieties such as the sprats, halmessa, sardines (salaya, soodaya), and herrings (hurulla). (2) the medium size pelagic species such as the mackerel (kumbala and bolla), barracuda (jeela), seer Spanish mackerel (thora), frigate mackeral (alagoduwa), mackerel tuna (atawalla) and the skipjack (balaya). (3) the large size fishes such as yellow fin tuna (kelawalla), big eye tuna, marlins (koppora and gappara), sail fish (thalapath), sharks (mora) and rays (maduwa). Production levels of exploited resources are noted, and seasonal patterns and annual in their abundance are considered. On the basis of observations and estimations of the existing fisheries, and the results of experimental fishing, figures are presented of the potential yield of those species already exploited. The development of that potential depends on the development of modern techniques of pole and line fishing, application of tuna longline and shark longline, increasing the number of units of drift nets and the introduction of a bait fishery for the longline and pole line fishery. Some features upon which the successes of any venture to exploit such resources are noted, particularly those which relate to the nature of the fishing vessels used.

Operatives for off-shore and deep-sea fishing vessels

The need for operatives for the offshore and deep-sea fishing vessels is currently very great, and the importance of fisheries training has long been recognized by the Department of Fisheries. In 1974 the Sri Lanka Fisheries Training Institute was established with Japanese aid. The author discusses the purpose and functions of the institute, the first batch of trainees from which finished their training in June 1977. Annexes describe the courses offered, diplomas and certificates, admission requirements, and the conditions required for candidates sitting second-hand fishing vessel examination of the Mercantile Marine Department, Bombay District.

Infrastructure facilities for deep-sea and off-shore fishing

The author presents a brief account of the infrastructure facilities required for the fishing industry. He describes those facilities presently available in Sri Lanka, and those that are under construction, and gives a few suggestions indicating the nature of infrastructure facilities that are vital to the local situation at its present stage of development. The principal facilities discussed are (1) fish landing places; (2) unloading handling facilities; (3) vessel servicing facilities; and (4) navigation aids.

Private sector to invest in off-shore fishing and to encourage the financial incentives to be introduced for deep-sea fishing in Sri Lanka

The author discusses the financial aspects of private sector investment in off-shore fishing, with particular attention to tax incentives, such as those offered to tourist hotels, which are considered applicable to the current case of fishing vessel purchase, and the operation of the vessels themselves, hiring, publicity and profitability. Tax incentives which were introduced to encourage investment in the tourist industry should be introduced to encourage investment in the fishing industry on the lines presented. It is essential that wide publicity be given for the incentives introduced so as to achieve the desired results.

Economic analysis of 22m and 23m deep sea trawlers

The cost-benefit analysis of 23m and 22m trawlers operated from Visakhapatnam base is presented. The study indicated that the deep sea fishing in Indian waters is a profitable venture. The profit over capital investment for a 23m trawler worked out to about 18% whereas the same was about 10% for a 22m trawler. Catch per trawling operation, cost of production, productivity per man year, energy yield etc. establish the economic superiority of 23m vessel.

Fish sauce from two species of unexploited deep sea fish Peristedion adeni and Peristedion weberi

Two species of unexploited deep sea fish Peristedion adeni and Peristedion weberi caught from the Exclusive Economic Zone of India were subjected to fermentation at ambient temperature (30 ± 2°C) in the presence of salt in 4:1 ratio. Periodical analysis of the fermented product was carried out up to one year. The sauces had brownish yellow colour and conformed to special grade of the standards prescribed by the Food and Drug Administration. Further solubilization of protein after 9 month's maturation was not appreciable.

Mineralogical variations and physical properties of deep sea sediments, northwest Arabian Sea

Charles Darwin the research ship undertook an Oceanographic Cruise in 1986, CD 86/17 of the North Arabian Sea. Sediment cores were collected between 15° and 25°N. In this study sediment cores collected from deep Indus and Oman basins (CD 1715, CD 1730, CD 1738) have been analyzed for mineralogy, water content and porosity. In general, the cores are mainly composed of clay to silt sized terrigenous and biogenic constituents. Quartz, Chlorite and Illite are the common minerals of Arabian Sea sediments. Porosity determined by water content of sediments has been correlated with quartz/chlorite and quartz/illite peak ratios to show a relationship between mineral composition and physical properties.

Thermo-hydro-geomechanical simulations of methane gas production from deep sea methane hydrate formations

A new constitutive model called Methane Hydrate Critical State (MHCS) model was conducted to investigate the geomechanical response of the gas-hydrate-bearing sediments at the Nankai Trough during the wellbore construction process. The strength and dilatancy of gas-hydrate-bearing soil would gradually disappear when the bonds are destroyed because of excessively shearing, which are often observed in dense soils and also in bonded soils such as cemented soil and unsaturated soil. In this study, the MHCS model, which presents such softening features, would be incorporated into a staged-finite-element model in ABAQUS, which mainly considered the loading history of soils and the interaction between cement-casing-formation. This model shows the influence of gas-hydrate-bearing soil to the deformation and stability of a wellbore and the surrounding sediments during wellbore construction. At the same time, the conventional Mohr-Coulomb model was used in the model to show the advantages of MHCS model by comparing the results of the two models.

Bioorganic/inorganic hybrid composition of sponge spicules: Matrix of the giant spicules and of the comitalia of the deep sea hexactinellid Monorhaphis

The giant basal spicules of the siliceous sponges Monorhaphis chuni and Monorhaphis intermedia (Hexactinellida) represent the largest biosilica structures on earth (up to 3 m long). Here we describe the construction (lamellar organization) of these spicules and of the comitalia and highlight their organic matrix in order to understand their mechanical properties. The spicules display three distinct regions built of biosilica: (i) the outer lamellar zone (radius: >300 mu m), (ii) the bulky axial cylinder (radius: <75 mu m), and (iii) the central axial canal (diameter: <2 mu m) with its organic axial filament. The spicules are loosely covered with a collagen net which is regularly perforated by 7-10 mu m large holes; the net can be silicified. The silica layers forming the lamellar zone are approximate to 5 mu m thick; the central axial cylinder appears to be composed of almost solid silica which becomes porous after etching with hydrofluoric acid (HF). Dissolution of a complete spicule discloses its complex structure with distinct lamellae in the outer zone (lamellar coating) and a more resistant central part (axial barrel). Rapidly after the release of the organic coating from the lamellar zone the protein layers disintegrate to form irregular clumps/aggregates. In contrast, the proteinaceous axial barrel, hidden in the siliceous axial cylinder, is set up by rope-like filaments. Biochemical analysis revealed that the (dominant) molecule of the lamellar coating is a 27-kDa protein which displays catalytic, proteolytic activity. High resolution electron microscopic analysis showed that this protein is arranged within the lamellae and stabilizes these surfaces by palisade-like pillars. The mechanical behavior of the spicules was analyzed by a 3-point bending assay, coupled with scanning electron microscopy. The load-extension curve of the spicule shows a biphasic breakage/cracking pattern. The outer lamellar zone cracks in several distinct steps showing high resistance in concert with comparably low elasticity, while the axial cylinder breaks with high elasticity and lower stiffness. The complex bioorganic/inorganic hybrid composition and structure of the Monorhaphis spicules might provide the blueprint for the synthesis of bio-inspired material, with unusual mechanical properties (strength, stiffness) without losing the exceptional properties of optical transmission. (C) 2007 Elsevier Inc. All rights reserved.

Vertical distribution of bacterial and archaeal communities along discrete layers of a deep-sea cold sediment sample at the East Pacific Rise (similar to 13 degrees N)

The community structure and vertical distribution of prokaryotes in a deep-sea (ca. 3,191 m) cold sediment sample (ca. 43 cm long) collected at the East Pacific Rise (EPR) similar to 13 degrees N were studied with 16SrDNA-based molecular analyses. Total community DNA was extracted from each of four discrete layers EPRDS-1, -2, -3 and -4 (from top to bottom) and 16S rDNA were amplified by PCR. Cluster analysis of DGGE profiles revealed that the bacterial communities shifted sharply between EPRDS-1 and EPRDS-2 in similarity coefficient at merely 49%. Twenty-three sequences retrieved from DGGE bands fell into 11 groups based on BLAST and bootstrap analysis. The dominant groups in the bacterial communities were Chloroflexi, Gamma proteobacteria, Actinobacterium and unidentified bacteria, with their corresponding percentages varying along discrete layers. Pairwise Fst (F-statistics) values between the archaeal clone libraries indicated that the archaeal communities changed distinctly between EPRDS-2 and EPRDS-3. Sequences from the archaeal libraries were divided to eight groups. Crenarchaea Marine Group I (MGI) was prevalent in EPRDS-1 at 83%, while Uncultured Crenarchaea group II B (UCII B) abounded in EPRDS-4 at 61%. Our results revealed that the vertically stratified distribution of prokaryotic communities might be in response to the geochemical settings and suggested that the sampling area was influenced by hydrothermalism. The copresence of members related to hydrothermalism and cold deep-sea environments in the microbial community indicated that the area might be a transitional region from hydrothermal vents to cold deep-sea sediments.

Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases

Six deep-sea proteolytic bacteria taken from Aleutian margin sediments were screened; one of them produced a cold-adapted neutral halophilic protease. These bacteria belong to Pseudoalteromonas spp., which were identified by the 16S rDNA sequence. Of the six proteases produced, two were neutral cold-adapted proteases that showed their optimal activity at pH 7-8 and at temperature close to 35 degrees C, and the other four were alkaline proteases that showed their optimal activity at pH 9 and at temperature of 40-45 degrees C. The neutral cold-adapted protease E1 showed its optimal activity at a sodium chloride concentration of 2 M, whereas the activity of the other five proteases decreased at elevated sodium chloride concentrations. Protease E1 was purified to electrophoretic homogeneity and its molecular mass was 34 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of protease E1 was determined to be 32,411 Da by mass spectrometric analysis. Phenylmethyl sulfonylfluoride (PMSF) did not inhibit the activity of this protease, whereas it was partially inhibited by ethylenediaminetetra-acetic acid sodium salt (EDTA-Na). De novo amino acid sequencing proved protease E1 to be a novel protein.

Diversity, abundance and distribution of amoA-encoding archaea in deep-sea methane seep sediments of the Okhotsk Sea

The authors would like to thank Jin Sun, Jian Sun, Liangliang Kong, Nianshuang Wang, Chunhui Wang, Linbao Zhang and Ying Zhang for their assistance in the project. This work was supported by China Ocean Mineral Resources R&D Association grants DYXM-115-02-2-20 and DYXM-115-02-2-6, Hi-Tech Research and Development Program of China grant 2007AA091903, China National Natural Science Foundation grant 40576069, National Basic Research Program of China grant 2009CB219506 and the Fundamental Research Funds for the Central Universities of China grant 09CX05005A. M. G. K. was funded by incentive funds provided by the UofL-EVPR office and the US National Science Foundation (EF-0412129).