Plasminogen deficiency accelerates vessel wall disease in mice predisposed to atherosclerosis

A critical link between hemostatic factors and atherosclerosis has been inferred from a variety of indirect observations, including the expression of procoagulant and fibrinolytic factors within atherosclerotic vessels, the presence of fibrin in intimal lesions, and the cellular infiltration of mural thrombi leading to their incorporation into developing plaques. To directly examine the role of the key fibrinolytic factor, plasminogen, in atherogenesis, plasminogen-deficient mice were crossed to hypercholesterolemic, apolipoprotein E-deficient mice predisposed to atherosclerosis. We report that the loss of plasminogen greatly accelerates the formation of intimal lesions in apolipoprotein E-deficient animals, whereas plasminogen deficiency alone does not cause appreciable atherosclerosis. These studies provide direct evidence that circulating hemostatic factors strongly influence vessel wall disease in the context of a disorder in lipid metabolism.

Fatal embryonic bleeding events in mice lacking tissue factor, the cell-associated initiator of blood coagulation.

Tissue factor (TF) is the cellular receptor for coagulation factor VI/VIIa and is the membrane-bound glycoprotein that is generally viewed as the primary physiological initiator of blood coagulation. To define in greater detail the physiological role of TF in development and hemostasis, the TF gene was disrupted in mice. Mice heterozygous for the inactivated TF allele expressed approximately half the TF activity of wild-type mice but were phenotypically normal. However, homozygous TF-/- pups were never born in crosses between heterozygous mice. Analysis of mid-gestation embryos showed that TF-/- embryos die in utero between days 8.5 and 10.5. TF-/- embryos were morphologically distinct from their TF+/+ and TF+/- littermates after day 9.5 in that they were pale, edematous, and growth retarded. Histological studies showed that early organogenesis was normal. The initial failure in TF-/- embryos appeared to be hemorrhaging, leading to the leakage of embryonic red cells from both extraembryonic and embryonic vessels. These studies indicate that TF plays an indispensable role in establishing and/or maintaining vascular integrity in the developing embryo at a time when embryonic and extraembryonic vasculatures are fusing and blood circulation begins.

Urokinase-type plasminogen activator is effective in fibrin clearance in the absence of its receptor or tissue-type plasminogen activator.

The availability of gene-targeted mice deficient in the urokinase-type plasminogen activator (uPA), urokinase receptor (uPAR), tissue-type plasminogen activator (tPA), and plasminogen permits a critical, genetic-based analysis of the physiological and pathological roles of the two mammalian plasminogen activators. We report a comparative study of animals with individual and combined deficits in uPAR and tPA and show that these proteins are complementary fibrinolytic factors in mice. Sinusoidal fibrin deposits are found within the livers of nearly all adult mice examined with a dual deficiency in uPAR and tPA, whereas fibrin deposits are never found in livers collected from animals lacking uPAR and rarely detected in animals lacking tPA alone. This is the first demonstration that uPAR has a physiological role in fibrinolysis. However, uPAR-/-/tPA-/- mice do not develop the pervasive, multi-organ fibrin deposits, severe tissue damage, reduced fertility, and high morbidity and mortality observed in mice with a combined deficiency in tPA and the uPAR ligand, uPA. Furthermore, uPAR-/-/tPA-/- mice do not exhibit the profound impairment in wound repair seen in uPA-/-/tPA-/- mice when they are challenged with a full-thickness skin incision. These results indicate that plasminogen activation focused at the cell surface by uPAR is important in fibrin surveillance in the liver, but that uPA supplies sufficient fibrinolytic potential to clear fibrin deposits from most tissues and support wound healing without the benefit of either uPAR or tPA.

Charlotte Corday and certain men of the revolutionary torment,

Mode of access: Internet.

The principal speeches of the statesmen and orators of the French revolution, 1789-1795;

v. 1. Mirabeau. Vergniaud. Gensonné. Guadet. Louvet. Cambon.--v. 2. Barère. Danton. Robespierre. Saint-Just. Baudin.

Les hommes de la rev́olution--

Mirabeau -- Danton -- Vergniaud.

貧者に途を拓け

Mode of access: Internet.

Napoleon and the end of the French revolution,

"This book is a sequel to my works on Mirabeau, Danton, and Robespierre and their part in the French revolution." --Pref.

Die Ahnfrau,

"A partial bibliography": p. lii-lix.

O JORNALISMO POLÍTICO NO BRASIL: SUA TRAJETÓRIA

Esta dissertação tem como tema o jornalismo político e sua essência. O estudo parte de uma investigação que pretende responder a seguinte questão: Seria a política a essência do jornalismo? Recorro aos autores clássicos da sociologia e da literatura (Antonio Candido, Caio Prado Junior, Celso Furtado, Gilberto Freyre, Sérgio Buarque de Holanda e Sílvio Romero) com o objetivo de resgatar as bases de nossa formação política e econômica e compreender em qual contexto surge a imprensa brasileira. O modelo de colonização e os interesses políticos das classes dominantes, conforme apontado pelos clássicos da sociologia, constituem os alicerces nos quais o jornalismo político se consolidou, oferecendo evidências de que a essência do jornalismo é a política.(AU)

Exposición a monóxido de carbono en trabajadores de control vehicular-Cuenca: estudio exploratorio

El presente estudio exploratorio evalúa la exposición al monóxido de carbono (CO), expresado en porcentaje de carboxihemoglobina (%COHb), entre los trabajadores de los centros de control vehicular (CRV) DANTON-Cuenca. Todos los trabajadores (n=55) de los CRVs de Capulispamba y Mayancela participaron en este estudio. El %COHb se determinó a partir de muestras de sangre tomadas al inicio y final de un día laboral de febrero, abril y julio de 2013. Los resultados muestran que el %COHb se incrementó del inicio al final de la jornada laboral en ambos CRVs tanto en el grupo de trabajadores que fuman y no fumadores (P<0.001). Entre los no fumadores (N=42), el %COHb al final de la jornada sobrepasó el valor límite biológico (VLB) para COHb (3.5%) únicamente en el CRV de Mayancela (4.12%, P<0.05). Entre los fumadores (N=13), los promedios de %COHb al final de la jornada sobrepasaron los VLB en ambos CRVs. Se concluye, que los trabajadores de los CRVs de Mayancela y aquellos que fuman presentan %COHb mayores al VLB.

INDÚSTRIA SUCRO-ALCOOLEIRA NA ARGENTINA, NO BRASIL E O CONTEXTO DOS BIO-COMBUSTÍVEIS NO CONTINENTE AMERICANO

O objetivo desse artigo é abordar aspectos relacionados à produção e ao consumo de biocombustíveis – sobretudo bio-etanol - no continente americano, analisando as seguintes áreas: Estados Unidos; América Central, Caribe e América do Sul – com maior ênfase para Brasil e Argentina. Para nos atentarmos ao tamanho limite de páginas, deixaremos de abordar algumas experiências interessantes, como as de Peru, Colômbia, México, Guatemala e Guiana, entre outras. 

A TERRITORIALIZAÇÃO DA PECUÁRIA DE CORTE NO OESTE PAULISTA

Este trabalho apresenta a ação hegemônica, desde a década de 1950, que a pecuária bovina de corte exerce nas áreas rurais do oeste paulista (SP). Enquanto uma totalidade parcial do espaço geográfico em que atua o setor, a região se transformou em um referencial nacional na tomada de preços dos bovinos em peso de abate. No atual processo de deslocamento da boiada nacional do Centro-Sul para o Norte do país, neste trabalho se retrata a participação dos agropecuaristas regionais nesse desenrolar. Mesmo com a diminuição do percentual da boiada nacional presente nas áreas agrícolas da região, dos escritórios localizados nos edifícios agropecuários de Araçatuba, Presidente Prudente e Ribeirão Preto, a elite ruralista dá o encaminhamento de suas possessões na longínqua frente de expansão da atividade em terras setentrionais do Brasil.