64 resultados para DIPSOSAURUS-DORSALIS


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An FAO/IAEA Co-ordinated Research Project (CRP) on “Resolution of Cryptic Species Complexes of Tephritid Pests to Overcome Constraints to SIT Application and International Trade” was conducted from 2010 to 2015. As captured in the CRP title, the objective was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. The scientific output was the accurate alignment of biological species with taxonomic names; which led to the applied outcome of assisting FAO and IAEA Member States in overcoming technical constraints to the application of the Sterile Insect Technique (SIT) against pest fruit flies and the facilitation of international agricultural trade. Close to 50 researchers from over 20 countries participated in the CRP, using coordinated, multidisciplinary research to address, within an integrative taxonomic framework, cryptic species complexes of major tephritid pests. The following progress was made for the four complexes selected and studied: Anastrepha fraterculus complex – Eight morphotypes and their geographic and ecological distributions in Latin America were defined. The morphotypes can be considered as distinct biological species on the basis of differences in karyotype, sexual incompatibility, post-mating isolation, cuticular hydrocarbon, pheromone, and molecular analyses. Discriminative taxonomic tools using linear and geometric morphometrics of both adult and larval morphology were developed for this complex. Bactrocera dorsalis complex – Based on genetic, cytogenetic, pheromonal, morphometric, and behavioural data, which showed no or only minor variation between the Asian/African pest fruit flies Bactrocera dorsalis, B. papayae, B. philippinensis and B. invadens, the latter three species were synonymized with B. dorsalis. Of the five target pest taxa studied, only B. dorsalis and B. carambolae remain as scientifically valid names. Molecular and pheromone markers are now available to distinguish B. dorsalis from B. carambolae. Ceratitis FAR Complex (C. fasciventris, C. anonae, C. rosa) – Morphology, morphometry, genetic, genomic, pheromone, cuticular hydrocarbon, ecology, behaviour, and developmental physiology data provide evidence for the existence of five different entities within this fruit fly complex from the African region. These are currently recognised as Ceratitis anonae, C. fasciventris (F1 and F2), C. rosa and a new species related to C. rosa (R2). The biological limits within C. fasciventris (i.e. F1 and F2) are not fully resolved. Microsatellites markers and morphological identification tools for the adult males of the five different FAR entities were developed based on male leg structures. Zeugodacus cucurbitae (formerly Bactrocera (Zeugodacus) cucurbitae) – Genetic variability was studied among melon fly populations throughout its geographic range in Africa and the Asia/Pacific region and found to be limited. Cross-mating studies indicated no incompatibility or sexual isolation. Host preference and genetic studies showed no evidence for the existence of host races. It was concluded that the melon fly does not represent a cryptic species complex, neither with regard to geographic distribution nor to host range. Nevertheless, the higher taxonomic classification under which this species had been placed, by the time the CRP was started, was found to be paraphyletic; as a result the subgenus Zeugodacus was elevated to genus level.

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Laboratory colonies of Bactrocera passiflorae (Froggatt) and B. xanthodes (Broun) were established at Koronivia Research Station, Fiji in 1991. Laboratory rearing of the two economically important species was a prerequisite to studies conducted on protein bait spray and quarantine treatment development. To increase the production of laboratory reared fruit flies for this research and also to have a substitute larval diet available, replicated comparisons of the effectiveness of larval diets were carried out using B. passiflorae and B. xanthodes. The diets compared were pawpaw/bagasse, dehydrated carrot and diets used for culturing Mediterranean fruit fly (Ceratitis capitata Wiedemann), Oriental fruit fly (B. dorsalis Hendel), melon fly (B. cucurbitae Coquillett) and B. latifrons (Hendel), pawpaw diet and breadfruit diet. B. passiflorae and B. xanthodes eggs seeded onto the various diets were allowed to develop into larvae, pupae and adults. The percentage egg hatch, number of pupae recovered, percentage pupal mortality, weight of 100 pupae, number of adults and percentage eclosion were used to determine the effectiveness of the diets. Results showed that pawpaw/bagasse and dehydrated carrot diets performed favorably for both species. The pawpaw diet currently used as standard larval diets for both species is the most readily available and easiest to use. Breadfruit diet was tested on B. xanthodes only and showed that it was a suitable substitute for the pawpaw-based diets. Other larval diets, cassava/pawpaw and banana diets, that have been developed and used in the South Pacific areas are also discussed in this paper. When pawpaw or breadfruit are not available, dehydrated carrot diet may be substituted for fruit-based larval diets.

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Fruit flies that belong to the genus Bactrocera (Diptera: Tephritidae) are major invasive pests of agricultural crops in Asia and Australia. Increased transboundary movement of agricultural produce has resulted in the chance introduction of many invasive species that include Bactrocera mainly as immature stages. Therefore quick and accurate species diagnosis is important at the port of entry, where morphological identification has a limited role, as it requires the presence of adult specimens and the availability of a specialist. Unfortunately when only immature stages are present, a lacunae in their taxonomy impedes accurate species diagnosis. At this juncture, molecular species diagnostics based on COX-I have become handy, because diagnosis is not limited by developmental stages. Yet another method of quick and accurate species diagnosis for Bactrocera spp. is based on the development of species-specific markers. This study evaluated the utility of COX-I for the quick and accurate species diagnosis of eggs, larvae, pupae and adults of B. zonata Saunders, B. tau Walker, and B. dorsalis Hendel. Furthermore the utility of species-specific markers in differentiating B. zonata (500bp) and B. tau (220bp) was shown. Phylogenetic relationships among five subgenera, viz., Austrodacus, Bactrocera, Daculus, Notodacus and Zeugodacus have been resolved employing the 5' region of COX-I (1490-2198); where COX-I sequences for B. dorsalis Hendel, B. tau Walker, B. correcta Bezzi and B. zonata Saunders from India were compared with other NCBI-GenBank accessions. Phylogenetic analysis employing Maximum Parsimony (MP) and Bayesian phylogenetic approach (BP) showed that the subgenus Bactrocera is monophyletic.

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Environment-friendly management of fruit flies involving pheromones is useful in reducing the undesirable pest populations responsible for decreasing the yield and the crop quality. A nanogel has been prepared from a pheromone, methyl eugenol (ME) using a low-molecular mass gelator. This was very stable at open ambient conditions and slowed down the evaporation of pheromone significantly. This enabled its easy handling and transportation without refrigeration, and reduction in the frequency of pheromone recharging in the orchard. Notably the involvement of the nano-gelled pheromone brought about an effective management of Bactrocera dorsalis, a prevalent harmful pest for a number of fruits including guava. Thus a simple, practical and low cost green chemical approach is developed that has a significant potential for crop protection, long lasting residual activity, excellent efficacy and favorable safety profiles. This makes the present invention well-suited for pest management in a variety of crops.

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It is known that the larvae of Chironomidae in the first stages of life after leaving the egg case, swim for a long time in a body of water. Positive reaction in light, the capability of directed swimming and passive floating in suspension allow the larvae to temporarily carry out a planktonic way of life. This study describes the behaviour of Chironomus dorsalis larvae after leaving the egg case. The feeding of chironomid larvae in the first stages of development was also described.

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柑桔小实蝇 Dacus (bactrocera) dorsalis (Hendel)在云南景谷1年发生5代,以老熟幼虫和蛹在土壤中越冬或越夏。2月中至8月中旬,成虫产卵于芒果果实内,致使芒果腐烂。室内用40%氧化乐果、50%杀螟松和80%的敌敌畏1 000、1 500倍液,对成虫的毒杀率达100%,幼虫达88.73%,蛹为10.0%。大田防治用1 000倍液,同时用性信息素进行诱杀,保果效果达95.74%。

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鰋鮡鱼类共包括9 属43 种(亚种),隶属于鲇形目(Siluriformes)鮡科 (Sisoridae)鰋鮡亚科(Glyptosternae)中鰋鮡族(Tribe Glyptosternini)的鰋鮡 亚族(Subtribe Glyptosternina)。鰋鮡鱼类形态上的共同特征为:无胸吸着器,胸、 腹鳍水平展开,第一根鳍条完全分节或在外缘生出许多软骨细条,被外表皮所裹, 在腹面看到的是许多与分节或软骨细条大致对应的横纹皱褶。鰋鮡鱼类集中分布 于青藏高原周边的水系中,部分属种向西分布到了中亚地区的阿姆河上游,是适 应山区急流环境的一群鱼类。近年来一系列的研究表明,鰋鮡鱼类为一单系类群, 它的起源和演化与青藏高原的隆升有着直接的关系。鰋鮡鱼类的系统发育研究, 可以为青藏高原隆升的年代、幅度和形式提供间接的证据,其系统发育树的拓扑 结构也直接反映了东喜马拉雅地区诸水系的形成与演变。 本研究对鰋鮡鱼类9 属进行了系统整理。1. 发现并描述了异齿鰋属 (Oreoglanis)两新种,分别为分布于景东无量山(澜沧江水系)的景东异齿鰋 (O. jingdongensis)和分布于怒江水系南景河和南滚河的无斑异齿鰋(O. immaculatus)。认为分布于中国的异齿鰋属鱼类均属于尖须异齿鰋种组(O. siamensis species group)。指出区分异齿鰋属两个种组:尖须异齿鰋种组和细尾 异齿鰋种组(O. delacouri species group)的特征是下唇中部是否具有中央缺刻, 尾型的差别(新月型尾或凹型尾)不能用来区分两个种组,给出了异齿鰋属的检 索表,并绘制了异齿鰋属鱼类分布图。2. 对鮡属(Pareuchiloglanis)鱼类进行了 系统整理,通过外部形态度量性状的比较,认为分布于澜沧江水系的,曾经被鉴 定为扁头鮡(P. kamengensis)的标本,应属于大鳍鮡(P. macropterus),扁头鮡 和大鳍鮡之间的最明显的差别在于腹鳍前长的不同。前者的腹鳍前长为体长的 53.2-64.9%,后者的腹鳍前长均不达体长的50%。3. 通过比较金沙江水系分布的 5 种鮡属鱼类,中华鮡(P. sinensis)、前臀鮡(P. anteanalis)、壮体鮡(P. robusta)、 四川鮡(P. sichuanensis)和天全鮡(P. tianquanensis),认为天全鮡和四川鮡之 间分布水系重叠,外部形态亦无差别,天全鮡很可能为四川鮡的同物异名。给出 了鮡属鱼类的系统检索表,并绘制了鮡属鱼类分布图。利用分子系统学的原理和方法对鰋鮡鱼类进行系统发育研究。测定了6 属 15 种鰋鮡鱼类和鮡科中非鰋鮡鱼类4 属7 种共28 个体的线粒体Cyt b 基因部分 片段和全序列(1138 bp),结合从GenBank 下载的相关类群相同的基因序列,以 魾属(Bagarius)的巨魾(B. yarrelli),纹胸鮡属(Glyptothorax)的穴形纹胸鮡 (G. cavia)、亮背纹胸鮡(G. dorsalis)、扎那纹胸鮡(G. zainaensis),福建纹胸 鮡(G. fukiensis fukiensis)、海南纹胸鮡(G. fukiensis hainanensis),黑鮡属(Gagata) 的长丝黑鮡(G. dolichonema)以及褶鮡属(Pseudecheneis)的黄斑褶鮡(P. sulcatus) 和无斑褶鮡(P. immaculatus)作为外类群,采用贝叶斯法(Bayesian)、最简约 法(maximum pasimony, MP)和邻接法(neighbour-joining, NJ)构建系统发育树。 结果显示: 1. 鰋鮡鱼类为一单系类群,并且与褶鮡属互为姐妹群关系; 2. 原鮡属、鰋属和凿齿鮡属是鰋鮡鱼类的三个基部类群; 3. 异齿鰋属构成为一个单系群,大鳍异齿鰋最早从该属的基部分化出来; 4. 石爬鮡属构成一个单系,并与分布于金沙江水系的中华鮡+前臀鮡构成姐妹 群,黄石爬鮡和青石爬鮡的单倍型相互交错;显示两个物种的分类是不合适的, 而是同一水系不同支流种群之间梯度变异的例子,依据本次研究所得出的三个分 支图,结合青石爬鮡自西至东分布于金沙江、雅砻江、大渡河、青衣江、岷江等 的分布格局以及形态特征的分布,显示形态特征的分布变化有以下趋势:自西至 东,腹鳍位置逐渐前移;颌须渐趋缩短;胸鳍趋向发达、伸达腹鳍起点。这些变 化趋势是同一水系不同支流种群之间梯度变异的极好例子; 5. 分布于澜沧江以西(包括澜沧江)水系的鮡属鱼类(扁头鮡、细尾鮡、短鳍 鮡)与分布于怒江和伊洛瓦底江水系的拟鰋属鱼类构成为一支,并且二者共同与 分布于元江上游(红河水系)的大孔鮡构成为单系。其中,短体拟鰋和拟鰋互为 姐妹种;而扁头鮡和短鳍鮡也互为姐妹种;细尾鮡的系统地位则尚不能确定; 6. 无论在MP 树、贝叶斯树还是NJ 树中,石爬鮡属、鮡属、拟鰋属和异齿鰋属 构成一个单系,并且支持率达到了100%。 7. 鰋属位于鰋鮡鱼类的基部,是较早就从鰋鮡鱼类祖先中演化出来的一个类群, 鰋类的口吸盘是一个趋同性状,是在急流环境条件下形成的一种适应性性状,在 鰋类各属中不是同源特征; 8. 鮡属鱼类不是一个单系类群,分布于金沙江流域的鮡属鱼类与同流域分布的石爬鮡属鱼类聚成一支;澜沧江及其以西水系分布的鮡属鱼类与同流域分布的拟 鰋属鱼类聚成一支,之后这两支又同异齿鰋属共同构成为一个大支。 结合鰋鮡鱼类的系统发育分支图对其动物地理学进行研究。鰋鮡鱼类是由类 似纹胸鮡属(Glyptothorax)鱼类的祖先演化而来,鰋属(Exostoma)和凿齿鮡 属(Glaridoglanis)是较早就从从类似原鮡的祖先中演化出来,鮡属、石爬鮡属、 拟鰋属和异齿鰋属这一大支由类似原鮡的祖先演化而来。在青藏高原强烈隆起等 重大地理隔离事件发生之前,类似于现生的原鮡属鱼类已经广布喜马拉雅山脉东 西两侧。在青藏高原强烈隆起等重大地理隔离事件发生后,鰋鮡鱼类在相同的地 理隔离下独自演化为现在的分布格局。

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通过对采自全国各地的4000多尾纹胸鮡属鱼类标本的分类整理,我国迄今共有该属鱼20种和亚种,其中新种3个,新记录3种。首次提出G. longinema Li和G. rubermentus Li为G. zainaensis Wu et al.的同物异名,G. trilineatoides Li为G. trilineatu Blyth的同物异名,G. fukiensis punctatum (Nichols)为G. fukiensis fukiensis (Rendahl)的同物异名。恢复了G. dorsalis Vinciguerra和 G. laosensis Fowler的有效地位。通过对大量标本的观察,皮肤表面突起结构及某些骨骼的形态具有种间分化明显、种内相对稳定的特点,是较好的分类性状,加上一些明显而稳定的体表色型、腹鳍的相对位置、偶鳍不分枝鳍条腹面有无细纹皮褶等,据此做出分种检索表。以骨骼性状及皮肤表面突起结构为主,结合多种其他性状作为检索特征系列,在本类群中尚属首次尝试。所有种的形态特征及分布均一一作了描述,并列出了主要文献及异名。

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We studied the morphology of three rare haptorid ciliates, using live observation and silver impregnation: Apertospathula verruculifera n. sp., Longispatha elegans n. gen., n. sp., and Rhinothrix porculus (Penard, 1922) n. gen., n. comb. Simple ethanol fixation (50-70%, v/v) is recommended to reveal the ciliary pattern of "difficult" ciliates, such as R. porculus, by protargol impregnation. The three genera investigated have a distinct feature in common, viz., a lasso-shaped oral bulge and circumoral kinety, where the right half is slightly to distinctly longer than the left and the circumoral kinety is open ventrally. Thus, they are united in a new spathidiid family, the Apertospathulidae n. fam., which probably evolved from a Bryophyllum-like ancestor by partial reduction of the oral bulge and circumoral kinety. Apertospathula verruculifera has a wart-like process, the palpus dorsalis, at the anterior end of the dorsal brush. The right branch of the circumoral kinety is only slightly longer than the left one. Longispatha elegans has a straight oral bulge and circumoral kinety, the right branch of which extends to the posterior end of the body while the left branch ends in the anterior third of the body. Rhinothrix porculus, a curious ciliate with a snout-like dorsal elongation of the oral bulge, the palpus oralis, has a highly characteristic ciliary pattern: the oral pattern is as in Longispatha, but the bulge and circumoral kinety extend spirally to the posterior end of the body while the somatic kinetics course meridionally. This is achieved by inserting some shortened kinetics in the curves of the oral bulge.

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Reduced arterial compliance precedes changes in blood pressure, which may be mediated through alterations in vessel wall matrix composition. We investigated the effect of the collagen type I-1 gene (COL1A1) +2046G>T polymorphism on arterial compliance in healthy individuals. We recruited 489 subjects (251 men and 238 women; mean age, 22.6±1.6 years). COL1A1 genotypes were determined using polymerase chain reaction and digestion by restriction enzyme Bal1. Arterial pulse wave velocities were measured in 3 segments, aortoiliac (PWVA), aortoradial (PWVB), and aorto-dorsalis-pedis (PWVF), as an index of compliance using a noninvasive optical method. Data were available for 455 subjects. The sample was in Hardy-Weinberg equilibrium with genotype distributions and allele frequencies that were not significantly different from those reported previously. The T allele frequency was 0.22 (95% confidence interval, 0.19 to 0.24). Two hundred eighty-three (62.2%) subjects were genotype GG, 148 (35.5%) subjects were genotype GT, and 24 (5.3%) subjects were genotype TT. A comparison of GG homozygotes with GT and TT individuals demonstrated a statistically significant association with arterial compliance: PWVF 4.92±0.03 versus 5.06±0.05 m/s (ANOVA, P=0.009), PWVB 4.20±0.03 versus 4.32±0.04 m/s (ANOVA, P=0.036), and PWVA 3.07±0.03 versus 3.15±0.03 m/s (ANOVA, P=0.045). The effects of genotype were independent of age, gender, smoking, mean arterial pressure, body mass index, family history of hypertension, and activity scores. We report an association between the COL1A1 gene polymorphism and arterial compliance. Alterations in arterial collagen type 1A deposition may play a role in the regulation of arterial compliance

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O presente estudo teve como objetivo a elaboração de uma chave de identificação ilustrada para diferenciar as espécies de camarões marinhos Dendrobranchiata, com ocorrência no litoral do Estado de São Paulo até a profundidade de 40m. As 13 espécies apresentadas neste trabalho foram obtidas mediante coletas mensais durante os anos de 1995 a 2000 na região de Ubatuba, SP . Nesta chave estão incluídas as espécies de interesse econômico, como os peneídeos Xiphopenaeus kroyeri, Farfantepenaeus brasiliensis, F. paulensis, Litopenaeus schmitti, Artemesia longinaris e o solenocerídeo Pleoticus muelleri. Além destas espécies, também foram adicionados os camarões que não são alvos das frotas pesqueiras, entre eles, Rimapenaeus constrictus, Acetes americanus, Peisos petrunkevitchi e os sicionídeos Sicyonia dorsalis, S. typica, S. laevigata e S. parri. A chave proposta servirá como uma ferramenta no auxílio da identificação dos camarões Dendrobranchiata, quer seja por pesquisadores ligados à área científica, como também por pessoas relacionadas aos órgãos responsáveis pelo controle da pesca, principalmente, na época do defeso.

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Mediante esta pesquisa, estudamos os arranjos configurados pela artéria celíaca em 30 patos domésticos Cairina moshata, sendo 20 machos e 10 fêmeas. Foi realizada a injeção de látex corado no sistema arterial e depois as peças foram fixadas em solução aquosa de formol a 10% para posterior dissecação dos vasos arteriais. A artéria celíaca nasce isoladamente da aorta descendente e fornece a artéria proventricular dorsal e a artéria esofágica e a seguir continua-se como tronco de dois outros ramos: esquerdo e direito. O ramo esquerdo emite um vaso que vasculariza a porção ventral do proventrículo (artéria proventricular ventral) e porção terminal do esôfago e, a seguir, fornece 3 ramos que vascularizam a face lateral esquerda e a margem cranial da moela ou ventrículo, bem como a junção pilórica na sua porção cranial. do vaso destinado à margem cranial da moela, surgem artérias ao lobo hepático esquerdo em número de 1 em 5 preparações (16,6% ± 6,8), 2 em 14 peças (46,66% ± 9,1) ou 3 em 8 peças (26,66% ± 8,1). O ramo direito envia inicialmente as artérias esplênicas cujo número foi de 2 em 6 preparações (20% ± 7,3), 3 em 12 peças (40% ± 8,9), 4 em 8 peças (26,6% ± 8,1), 5 em 3 peças (10% ± 5,5) e 6 em 1 preparação (3,33% ± 3,3). em seguida, emite colaterais ao lobo hepático direito que foram em número de 2 em 21 preparações (70% ± 8,4) ou 3 em 9 preparações (30% ± 8,4). Ainda, o ramo direito emite artérias endereçadas ao ceco esquerdo em número de 2 em 17 preparações (56,66% ± 9,1) ou 3 em 13 casos (43,33% ± 9,1). O ramo direito origina ainda 3 vasos endereçados à face lateral direita da moela (ventrículo), à margem caudal e à junção pilórica em sua porção mais caudal. A seguir, o ramo direito da artéria celíaca continua-se como artéria pancreaticoduodenal que supre o pâncreas e as porções ascendente e descendente do duodeno.

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This work aims to analyse the composition, abundance and distribution of the Penaeidea species which occur in the Ubatuba Bay (23 degrees 26'S and 45 degrees 02'W). The samples were monthly collected from October/1992 to September/1993. Each collect was composed of two parallel radials: the first (radial ''A'') was carried out in the mid region of the bay and the second one (radial ''B'') in the bay mouth. The trawls took one hour in a boat equipped with one otter-trawl (10 mm of mesh). The registered environmental factors were depth, bottom water temperature, granulometric composition and organic content of the sediment. After the trawls, the shrimp were separated from other marine organisms and counted. Eight species of shrimp were obtained: Xiphopenaeus kroyeri; Artemesia longinaris; Penaeus (L.) schmitti; P. (F.) brasiliensis; Trachypenaeus; constrictus; Sicyonia typica; S. dorsalis and Pleoticus muelleri. The most abundant species were X. kroyeri, A. longinaris, P. muelleri and T. constrictus. It was verified a very strong seasonality among the species. The X. kroyeri species occurred in both radials along the months but its abundance decreased from a November to March. Such fact is attributed to the temperature which reached a minimum value of 20 degrees C during this period. The species A. longinaris and P. muelleri were more frequent in the radial B which was caracterized by 14 +/- 1.3 m of depth, low organic content in the sediment (2.97%) and granulometric composition of medium sand. The distribution and abundance of these shrimps in the bay, beyond the hydrological features can be related to biotic factors as food availability, migration, inter and intra specific relations (competition, predation, etc.).

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An electromyographic study of the musculus interosseus dorsalis was performed on the right hand of 25 young adult male right-handed volunteers. The electrodes, simple coaxial needles, were implanted one at the ulnar head and the other at the radial head of the muscle. The muscles were analyzed during free movements of the index and against resistance. The same movements were done in four different positions of the fore-arm and hand, without variation in the results for each one of the movements. There was no significant difference between the activities of the ulnar head and radial head. During freely performed movements, muscle activity was recorded only during abduction. During movements against resistance, muscle activity was completely nil only during adduction; during the remaining movements, however, moderate (2+), strong (3+) and very strong activity (4+) was recorded.

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In this paper, we present evidence that the long-distance migratory catfish Brachyplatystoma filamentosum, B. flavicans, B. vaillantii, Goslinia platynema and Lithodoras dorsalis spawn in the headstreams of the Amazon river and its tributaries and that the estuary of the Amazon is the main nursery ground utilized by their alevins. The impact of hydroelectric dams on their populations are discussed in terms of interruption to fish migration routes up river and to larvae moving down river, provided that the hypothesis above holds. Ways of mitigating the impact are also considered. © 1991.