转基因鱼试验湖浮游生物群落DNA指纹拓扑结构与优势种的关系

针对多福转基因鱼试验湖的浮游生物群落进行了随机扩增多态性DNA(RAPD)研究,并与优势种类组成进行了相关分析．结果显示：9条引物共检测到76条长度在200—1200bp的谱带,其中共有带占5.3％,特有条带占40.8％,谱带多态率为94.7％；共观察到的25种优势种中有长额象鼻溞(Bosmina longirostris)、近邻剑水蚤(Cyclops vicinus)、螺形龟甲轮虫(Keratella cochlearis)、角甲藻(Ceratium hirundinella)和圆筒锥囊藻(Dinobr

浅水草型湖泊──扁担塘中桡足类的群落结构（英文）

本文研究了一浅水草型湖泊──扁担塘中桡足类的群落结构，包括种类组成、种群动态及现存量。在一周年的研究中，共发现14种浮游桡足类（9种剑水蚤和5种哲水蚤）。根据年平均密度，剑水蚤的优势种为：Mesocyclops notius，Cyclops vincinuis vincinus和Thermocyclops brevifurcatus，而哲水蚤的优势种为Meodiaptomus yantsekiangensis和Sinocalanus dorrii。通过比较长江沿岸的5个湖泊的桡足类的种类组成发现，桡足类的

武汉东湖桡足类生物量及生产量的初步研究

本文主要论述1980—1982年武汉东湖桡足类生物量及生产量的测算结果。三周年来在东湖Ⅰ、Ⅱ采样站上共观察到5种剑水蚤:广布中剑水蚤(Mesocyclops leuckarti)、近邻剑水蚤(Cyclops vicinus vicinus)、透明温剑水蚤(Thermocyclops hyalinus)、台湾温剑水蚤(Thermocyclops taihokuensis)、锯缘真剑水蚤(Eucyclops serrulatus)和5种哲水蚤:球状许水蚤(Schmackeria forbesi)特异荡镖水蚤(

Crustacean zooplankton distribution patterns and their biomass as related to trophic indicators of 29 shallow subtropical lakes

A comparative limnological study was carried out to present a snapshot of crustacean zooplankton communities and their relations to environmental factors to test whether there is a consistent relationship between crustacean biomass and trophic indicators among lake groups with similar trophic conditions. The study lakes showed a wide range of trophic status, with total phosphorus (TP) ranging from 0.008 to 1.448mgL(-1), and chlorophyll a from 0.7 to 146.1 mu g L-1, respectively. About 38 species of Crustacea were found, of which Cladocera were represented by 25 taxa (20 genera), and Copepoda by 13 taxa (I I genera). The most common and dominant species were Bosmina coregoni, Moina micrura, Diaphanosoma brachyurum, Cyclops vicinus, Thermocyclops taihokuensis, Mesocyclops notius and Sinocalanus dorrii. Daphnia was rare in abundance. Canonical correspondence analysis showed that except for four species (D. hyalina, S. dorrii, C. vicinus and M. micrura), almost all the dominant species had the same preference for environmental factors. Temperature, predatory cyclopoids and planktivorous fishes seem to be the key factors determining species distribution. TP was a relatively better trophic indicator than chlorophyll a to predict crustacean biomass. Within the three groups of lakes, however, there was no consistent relationship between crustacean biomass and trophic indicators. The possible reason might be that top-down and bottom-up control on crustaceans vary with lake trophic state. The lack of significant negative correlation between crustacean biomass and chlorophyll a suggests that there was little control of phytoplankton biomass by macrozooplankton in these shallow subtropical lakes. (c) 2007 Elsevier GmbH. All rights reserved.

Experimental study of trophic cascade effect of silver carp (Hypophthalmichthys molitrixon) in a subtropical lake, Lake Donghu: on plankton community and underlying mechanisms of changes of crustacean community

An enclosure experiment was carried out to test trophic cascade effect of filter-feeding fish on the ecosystem: growth of crustacean zooplankton, and possible mechanism of changes of crustacean community structure. Four fish biomass levels were set as follows: 0, 116, 176 and 316 g m(-2), and lake water ( containing ca. 190 g m(-2) of filter-feeding fishes) was comparatively monitored. Nutrient levels were high in all treatments during the experiment. Lowest algal biomass were measured in fishless treatment. Algal biomass decreased during days 21-56 as a function of fish biomass in treatments of low (LF), medium (MF) and high (HF) fish biomass. Crustaceans biomass decreased with increasing fish biomass. Small-bodied cladocerans, Moina micrura, Diaphanosoma brachyurum and Scapholeberis kingii survived when fish biomass was high whilst, large-bodied cladocerans Daphnia spp. and the cyclopoids Theromcyclops taihokuensis, T. brevifuratus, Mescyclops notius and Cyclops vicinus were abundant only in NF enclosures. Evasive calanoid Sinodiaptomus sarsi was significantly enhanced in LF, but decreased significantly with further increase of fish biomass. Demographic data indicated that M. micrura was well developed in all treatments. Our study indicates that algal biomass might be controlled by silver carp biomass in eutrophic environment. Changes of crustacean community are probably affected by the age of the first generation of species. Species with short generation time were dominant and species with long generation time survived less with high fish biomass. Evasive calanoids hardly developed in treatments with high fish biomass because of the ( bottle neck) effect of nauplii. Species abundance were positively related to fish predation avoidance. Other than direct predation, zooplankton might also be suppressed by filter-feeding fish via competition.

Long-term changes of planktonic rotifers in a subtropical Chinese lake dominated by filter-feeding fishes

1. The long-term changes (1956-1998) in density and species composition of planktonic rotifers were studied at two sampling stations (I, II) of Lake Donghu, a shallow eutrophic Chinese Lake densely stocked with filter-feeding fishes. Annual average densities of rotifers increased with an increase in fish yield and eutrophication, whilst species number decreased from 82 in 1962-1963 to 62 in 1994-1998. 2. During 1962-98, some species such as Anuraeopsis fissa, Polyarthra spp. (including P. dolichoptera & P. vulgaris), Trichocerca pusilla and Synchaeta oblonga increased their percentage in abundance remarkably, whilst the proportion of Keratella cochlearis decreased at two relatively eutrophic stations from 19 to 4.2% at Station I and from 30 to 3.2% at Station IL 3. The high r(max) of A. fissa probably made it more successful than other rotifers under high predation pressure by planktivorous fish. The decrease in the K. cochlearis population might be attributed partly to predation by Cyclops vicinus. 4. Small rotifers were less vulnerable to fish predation than large-sized cladocerans. Decreases in cladocerans coincided with increases in rotifers, suggesting that the indirect effect of fish predation on cladocerans might have partly contributed to the population development of rotifers in Lake Donghu during recent decades. 5. We also conducted surveys (1994-1998) of seasonal dynamics of rotifers at four sampling stations (I-IV) which have varied in trophic status after fragmentation of the lake in the 1960s. A total of 75 species were identified at the four stations. Both densities and biomass of rotifers were considerably higher in the two more eutrophic stations than in the two less eutrophic stations. This indicates that the population increase of rotifers at Stations I and II during recent decades might be partly attributed to eutrophication of the lake water.

Impacts of filter-feeding fishes on the long-term changes of crustacean zooplankton in a eutrophic subtropical Chinese lake

Long-term changes In the crustacean zooplankton community (calanoid and cyclopoid copepods and cladocerans) were studied in Lake Donghu, a shallow and eutrophic Chinese lake. This lake had been earlier stocked with two pump Alter-feeding Ashes, silver carp (Hypopthalmichthys molitrix) and bighead carp (Aristichthys nobilis). During the 1950s and the mid-1980s, the ratio of copepods to cladocerans was relatively stable but showed a general increase thereafter. From the early-1980s to the 1990s, calanoid/cyclopoid ratios decreased obviously. In the 1990s, Cyclops vicinus, Diaphanosoma brachyurum, and Moina micrura were dominant the abundance of C. vicinus and M. micrura increased significantly; and D, brachyurum showed a substantial decrease. The study shows that under extremely high pressure of Ash predation, the species which could recover rapidly from fish predation would be the most likely to survive and increase their numbers.

A zebrafish forebrain-specific zinc finger gene can induce ectopic dlx2 and dlx6 expression

Identifcation of the earliest forebrain-specific markers should facilitate the elucidation of molecular events underlying vertebrate forebrain determination and specification. Here we report the sequence and characterization of fez (forebrain embryonic zinc finger), a gene that is specifically expressed in the embryonic forebrain of zebrafish. Fez encodes a putative nuclear zinc finger protein that is highly conserved in Drosophila, zebrafish, Xenopus, mouse, and human. In zebrafish, the expression of fez becomes detectable at the anterior edge of the presumptive neuroectoderm by 70% epiboly. During the segmentation period, its expression is completely restricted to the rostral region of the prospective forebrain. At approximately 24 h postfertilization, fez expression is mostly confined to the telencephalon and the anterior-ventral region of the diencephalon. Although fez expression is present in one-eyed pinhead (oep) and cyclops (cyc) zebrfish mutants, the pattern is altered. Forced expression of fez induces ectopic expression of dlx2 and dlx6, two genes involved in brain development. Knockdown of fez function using a morpholino-based antisense oligo inhibited dlx2 expression in the ventral forebrain. Our studies indicate that fez is one of the earliest markers specific for the anterior neuroectoderm and it may play a role in forebrain development by regulating Dlx gene expression. (C) 2001 Academic Press.

Long-term changes in crustacean zooplankton and water quality in a shallow, eutrophic Chinese lake densely stocked with fish

From surveys made in 1962-1963, 1973-1974, 1979-1996 at two Stations in Lake Donghu, a shallow eutrophic water body near Wuhan, P. R. China, the authors, derive long-term changes in species composition, standing crop and body-size of planktonic crustaceans. The species number decreased from the 1960s to the 1990s. The cladocerans dropped from 46 (1960s) to 26 (1980s) to 13 (1990s); the copepods decreased from 14 (1960s) to 10 (1980s) to 7 (1990s). From the mid-1980s on, the dominant crustaceans also changed: Daphnia hyalina and D. carinata ssp. were replaced by Moina micrura and Diaphanosoma brachyurum at Stations 1 and 2, respectively; Cyclops vicinus replaced Mesocyclops leuckarti. Densities and biomass of Cladocera decreased markedly after 1987. Annual average densities and biomass of cladocerans were statistically differences between 1962-1986 and 1987-1996 (P < 0.01). Annual average densities of Daphnia (Station 1 + Station 2) were negatively correlated with fish yield. Since the 1980s, annual average body length of Cladocera and Calanoida decreased, while annual average body length of Cyclopoida increased. In the same years, average body length of copepods was lower during May-October than during January-April and November-December. A 12-yr data analysis showed annual average concentration of chlorophyll-a (Chl-a) to be negatively correlated with annual average density of Daphnia, whilst lake transparency was positively correlated with annual average densities of Daphnia. The results imply that, since Daphnia feeds efficiently on phytoplankton, it could decrease concentration of Chl-a, and enhance water transparency.

中国管口苔属（Solenostoma Mitt.）植物研究

本文是对中国管口苔属（Solenostoma Mitt.）植物种类的初步整理和研究。共记载3亚属、44种及1变种，其中有24个新组合，如下：Solenostoma (L.) subulatum (Evans) Wang, S. (L.) atrovirens (Schleich) Wang, S. (L.) grossitextum (Steph.) Wang, S. (S.) fusiformis (Steph.) Wang, S. (S.) lanigerum (Mitt.) Wang, S. (S.) pseudocyclops (Inoue) Wang, S. (S.) subrubrum (Steph.) Wang, S. (S.) pyriflorum var.minutissimum (Amak.) Wang, S. (S.) sinensis (Nich.) Wang, S. (S.) faurianum (Beauvd) Wang, S. (S.) cyclops (HAtt.) Wang, S. (S.) apressifolium (Mitt.) Wang, S. (S.) schaulianum (Steph.) Wang, S. (P.) Japonicum (Amak.) Wang, S. (P.) tetragonum (Lindb.) Wang, S. (P.) rosulans (Steph.) Wang, S. (P.) thermarum (Steph.) Wang, S. (P.) hattorianum (Amak.) Wang, S. (P.) rupicolum (Amak.) Wang, S. (P.) rubripunctatum (Hatt.) Wang, S. (P.) hasskarlianum (Nees) Wang, S. (P.) setulosum (Herzog) Wang, S. (P.) truncatum (Nees) Wang, S. (P.) tsukushiensis (Amak.) Wang, 其中有14种及1变种为中国分布新纪录，如下：Solenostoma (L.) grossitextum (Steph.) Wang, S. (S.) fusiformis (Steph.) Wang, S. (S.) faurianum (Beauvd.) Wang, S. (S.) cyclops (Hatt.) Wang, S. (S.) pusillum (Jens) Steph., S. (S.) pyriflorum var. minutissimum (Amak.) Wang, S. (P.) rubripunctatum (Hatt.) Wang, S. (P.) thermarum (Steph.) Wang, S. (P.) rosulans (Steph.) Wang, S. (P.) hyalinum (Lyell) Mitt., S. (P.) radicellosum Mitt, S. (P.) Japonicum (Amak.) Wang, S. (P.) tsukushiensis (Amak.) Wang, S. (P.) hatterianum (Amak.) Wang, S. (P.) rupicolum (Amak.) Wang, 其中还有6种中国特有种，如下：Solenostoma (L.) subulatum (Evans) Wang, S. (P.) flagellalioides Gao, S. (P.) microphyllum Gao, S. (P.) orbicularifolium Gao, S. (P.) setulosum (Herzog) Wang, S. (S.) sinensis (Nich.) Wang。本文还对管口苔属（Solenostoma Mitt.）的研究历史作了回顾。对中国管口苔属各分类群的鉴别特征、产地和地理分布作了叙述，对区系成份做了分析。并对前人工作中的某些问题进行了讨论，提出了自己的看法。

SOCIO-ENVIRONMENTAL FACTORS ASSOCIATED WITH THE DISTRIBUTION OF DRACUNCULUS MEDINENSIS (GUINEA WORM DISEASE) AND SUGGESTED METHODS FOR CONTROL, IBARAPA, NIGERIA, 1982 (AFRICA)

A population-based cross-sectional survey of socio-environmental factors associated with the prevalence of Dracunculus medinensis (guinea worm disease) was conducted in Idere, a rural agricultural community in Ibarapa, Oyo state, Nigeria, during 1982.^ The epidemiologic data were collected by household interview of all 501 households. The environmental data were collected by analysis of water samples collected from all domestic water sources and rainfall records.^ The specific objectives of this research were to: (a) Describe the prevalence of guinea worm disease in Idere during 1982 by age, sex, area of residence, drinking water source, religion and weekly amount of money spent by the household to collect potable drinking water. (b) Compare the characteristics of cases with non-cases of guinea worm in order to identify factors associated with high risk of infection. (c) Investigate domestic water sources for the distribution of Cyclops. (d) Determine the extent of potable water shortage with a view to identifying factors responsible for such shortage in the community. (e) Describe the effects of guinea worm on school attendance during 1980/1982 school years by class and location of school from piped water supply.^ The findings of this research indicate that during 1982, 31.8 percent of Idere's 6,527 residents experienced guinea worm infection, with higher prevalence of infection recorded in males in their most productive years and females in their teenage years. The role of sex and age to risk of higher infection rate was explained in the context of water related exposure and water intake due to dehydration from physical occupational actitives of subgroups.^ Potable water available to residents was considerably below the minimum recommended by WHO for tropical climates, with sixty-eight percent of water needs of the residents coming from unprotected surface water which harbour Cyclops, the obligatory intermediate host of Dracunculus medinensis. An association was found between periods of relative high density of Cyclops in domestic water and rainfall.^ Impact of guinea worm infection on educational activities was considerable and its implications were discussed, including the implications of the research findings in relation to control of guinea worm disease in Ibarapa. ^

Abundance and biomass of zooplankton from 1986-1994 collected in front of Constanta city, Black Sea

The Est Constanta 1986-1994 dataset contains zooplankton data collected allong a 5 station transect in front of the city Constanta (44°10'N, 28°41.5'E - EC1; 44°10'N, 28°47'E - EC2; 44°10'N, 28°54'E - EC3; 44°10'N, 29°08'E - EC4; 44°10'N, 29°22'E - EC5). Zooplankton sampling was undertaken at 5 stations where samples were collected using a Juday closing net in the 0-10, 10-25, 25-50m layer (depending also on the water masses). The dataset includes samples analysed for mesozooplankton species composition and abundance. Sampling volume was estimated by multiplying the mouth area with the wire length. Taxon-specific mesozooplankton abundance was count under microscope. Total abundance is the sum of the counted individuals. Total biomass Fodder, Rotifera , Ctenophora and Noctiluca was estimated using a tabel with wet weight for each species an stage.

Abundance and biomass of mesozooplankton from the Romanian littoral during DANUBS_2000 in spring and autumn 2000

The Danubs 2000 dataset contains zooplankton data collected in April, June. October and November 2000 in 11 station allong 5 transect in front of the Romanian littoral. Zooplankton sampling was undertaken at 11 stations where samples were collected using a Juday closing net in the 0-10, 10-25, and 25-50m layer (depending also on the water masses). The dataset includes samples analysed for mesozooplankton species composition and abundance. Sampling volume was estimated by multiplying the mouth area with the wire length. Taxon-specific mesozooplankton abundance was count under microscope. Total abundance is the sum of the counted individuals. Total biomass Fodder, Rotifera , Ctenophora and Noctiluca was estimated using a tabel with wet weight for each species an stage.

Abundance and biomass of zooplankton from 1981-1985 collected in front of Constanta city, Black Sea

The Est Constanta 1981-1985 dataset contains zooplankton data collected allong a 5 station transect in front of the city Constanta (44°10'N, 28°41.5'E - EC1; 44°10'N, 28°47'E - EC2; 44°10'N, 28°54'E - EC3; 44°10'N, 29°08'E - EC4; 44°10'N, 29°22'E - EC5). Zooplankton sampling was undertaken at 5 stations where samples were collected using a Juday closing net in the 0-10, 10-25, 25-50m layer (depending also on the water masses). The dataset includes samples analysed for mesozooplankton species composition and abundance. Sampling volume was estimated by multiplying the mouth area with the wire length. Taxon-specific mesozooplankton abundance was count under microscope. Total abundance is the sum of the counted individuals. Total biomass Fodder, Rotifera , Ctenophora and Noctiluca was estimated using a tabel with wet weight for each species an stage.

Abundance and biomass of zooplankton from the Romanian littoral collected in April, May and June 1997

The SHELF 1997 dataset contains zooplankton data collected in April, May and June 1997 5 transect in front of the Romanian littoral . Zooplankton sampling was undertaken using a Juday closing net in the 0-10, 10-25, and 25-50m layer (depending also on the water masses). The dataset includes samples analysed for mesozooplankton species composition and abundance. Sampling volume was estimated by multiplying the mouth area with the wire length. Taxon-specific mesozooplankton abundance was count under microscope. Total abundance is the sum of the counted individuals. Total biomass Fodder, Rotifera , Ctenophora and Noctiluca was estimated using a tabel with wet weight for each species an stage.