Electrospray tandem mass spectrometry of mixed-sequence RNA/DNA oligonucleotides

The fragmentation of electrospray-generated multiply deprotonated RNA and mixed-sequence RNA/DNA pentanucleotides upon low-energy collision-induced dissociation (CID) in a hybrid quadrupole time-of-flight mass spectrometer was investigated. The goal of unambiguous sequence identification of mixed-sequence RNA/DNA oligonucleotides requires detailed understanding of the gas-phase dissociation of this class of compounds. The two major dissociation events, base loss and backbone fragmentation, are discussed and the unique fragmentation behavior of oligoribonucleotides is demonstrated. Backbone fragmentation of the all-RNA pentanucleotides is characterized by abundant c-ions and their complementary y-ions as the major sequence-defining fragment ion series. In contrast to the dissociation of oligodeoxyribonucleotides, where backbone fragmentation is initiated by the loss of a nucleobase which subsequently leads to the formation of the w- and [a-base]-ions, backbone dissociation of oligoribonucleotides is essentially decoupled from base loss. The different behavior of RNA and DNA oligonucleotides is related to the presence of the 2'-hydroxyl substituent, which is the only structural alteration between the DNA and RNA pentanucleotides studied. CID of mixed-sequence RNA/DNA pentanucleotides results in a combination of the nucleotide-typical backbone fragmentation products, with abundant w-fragment ions generated by cleavage of the phosphodiester backbone adjacent to the deoxy building blocks, whereas backbone cleavage adjacent to ribonucleotides induces the formation of c- and y-ions. (C) 2002 American Society for Mass Spectrometry.

The Boston Handel and Haydn Society collection of church music : being a selection of the most approved psalm and hymn tunes, anthems, sentences, chants, &c. : together with many beautiful extracts from the works of Haydn, Mozart, Beethoven, and other eminent composers, harmonized for three and four voices, with a figured base for the organ and piano forte /

Mode of access: Internet.

History of the Handel and Haydn Society, of Boston, Massachusetts ... From the foundation of the society ... 1815 [to May 25, 1903] ...

Mode of access: Internet.

Bulletin of the Newton Natural History Society.

No more published.

Early voyages to America : a paper read before the Rhode Island Historical Society /

The same paper appears to have been published by the Old Colony Historical society (Taunton, Mass.), (OCLC#: 11551727; also cf. # 14939728).

Exhibition of contemporary German art; the Copley society of Boston 1909.

"Contemporary German art, by Paul Clemen": p. 5-33.

Essex Historical Society. Incorporated June 11, 1821.

In 1848 the Essex County Natural History Society and the Essex Historical Society united to form the Essex Institute.

Some passages in the history of the First Church in connexion with the Shepard Congregational Society in Cambridge : together with its confession of faith, form of admission, ecclesiastical principles, and rules, and names of members.

Mode of access: Internet.

A discourse delivered before the Pilgrim Society, at Plymouth, on the twenty second day of December, 1829. ...

Buff printed paper cover has title: "Mr. Sullivan's discourse, delivered at Plymouth."

Constitution of the Pilgrim Society, as amended May 1836; together with a list of members. Adopted as amended.

Mode of access: Internet.

The constitutional articles of the Pilgrim Society, incorporated February 24, 1820.

Mode of access: Internet.

An address, delivered January 24, 1830, in the Second Baptist Meetinghouse in Haverhill, preparatory to the organization of the East-Haverhill Temperance Society. ...

Mode of access: Internet.

The Washingtonian reform : an address, delivered before the Hingham Total Abstinence Society, June 16, 1844 /

"Published by request of the Government of the Society."

An extract from the Report of the directors of Massachusetts State Prison, made to his excellency the governor and the honourable council. October 18, 1827. : Reviewing certain parts of the Second annual report of the Prison Discipline Society.

Mode of access: Internet.

Vital records of Marblehead, Massachusetts, to the end of the year 1849 ...

Mode of access: Internet.