Construção de uma biblioteca de fragmentos de anticorpos monoclonais de galinhas com cadeia única (scFv) por phage display com reatividade cruzada para estirpes heterólogas do vírus da bronquite infecciosa aviária

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Patogenicidade cruzada de Ceratobasidium spp. do caquizeiro (Diospyros kaki) e do chá(Camellia sinensis) e reação de cultivares de caqui ao patógeno

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biossegurança e odontologia: crenças e atitudes de graduandos sobre o controle da infecção cruzada

Este estudo teve o objetivo de investigar as percepções de graduandos de Odontologia sobre a fidelidade às diretrizes de biossegurança e acerca do preservar-se. Nove questões abertas, que abordaram aspectos de interesse para o tema, foram aplicadas em entrevista com 14 acadêmicos, que realizavam atendimento odontológico de pacientes da Faculdade de Odontologia de Araraquara da Unesp. Utilizou-se a metodologia de pesquisa qualitativa e a estratégia metodológica para análise das entrevistas foi a Discurso do Sujeito Coletivo (DSC). Três figuras metodológicas foram obtidas, sendo ideias-centrais, expressões-chave e o DSC propriamente dito. A análise dos discursos permitiu avaliar a fala natural da coletividade. Verificou-se a adesão dos entrevistados aos protocolos de biossegurança, embora houvesse a queixa de que, na rotina diária, as precauções fossem negligenciadas por não serem muito práticas. Entre as medidas de proteção individual e coletiva, rotineiramente utilizadas, foram apontados o uso de Equipamento de Proteção Individual (EPI) e as barreiras protetoras, bem como as atividades de desinfecção e esterilização. O risco de contágio foi visto por alguns com pavor e por outros com total indiferença porque acreditavam ser algo do qual é possível de se ter controle por meio da adesão às precauções padrão. Entre as doenças de maior preocupação, a aids e as hepatites B e C foram as mais temidas. Diante do discurso obtido, salienta-se a necessidade de se aperfeiçoar as estratégias educacionais, com intuito de motivar a fiel adesão às normas de biossegurança, essenciais no trato de pacientes odontológicos.

Validação cruzada com correção de autovalores e regressão isotônica nos modelos de efeitos principais aditivos e interação multiplicativa

This paper presents an application of AMMI models - Additive Main effects and Multiplicative Interaction model - for a thorough study about the effect of the interaction between genotype and environment in multi-environments experiments with balanced data. Two methods of crossed validation are presented and the improvement of these methods through the correction of eigenvalues, being these rearranged by the isotonic regression. A comparative study between these methods is made, with real data. The results show that the EASTMENT & KRZANOWSKI (1982) method selects a more parsimonious model and when this method is improved with the correction of the eigenvalues, the number of components are not modified. GABRIEL (2002) method selects a huge number of terms to hold back in the model, and when this method is improved by the correction of eigenvalue, the number of terms diminishes. Therefore, the improvement of these methods through the correction of eigenvalues brings a great benefit from the practical point of view for the analyst of data proceeding from multi-ambient, since the selection of numbers of multiplicative terms represents a profit of the number of blocks (or repetitions), when the model AMMI is used, instead of the complete model.

Mordida cruzada posterior: uma classificação mais didática

O objetivo deste trabalho é apresentar uma nova classificação de mordida cruzada posterior, sendo esta mais didática e de fácil entendimento, diferindo das demais em sua nomenclatura, preservando, no entanto, os princípios fundamentais que regem a Ortodontia. Essa classificação torna o processo de diagnóstico da mordida cruzada posterior sistematizado, tornando-o mais preciso, auxiliando o clínico na elaboração de um plano de tratamento adequado e, como conseqüência, levando a um prognóstico mais favorável dessa má oclusão.

Estudo da reatividade cruzada em amostras de soro de cães positivos para Leishmania sp., Babesia canis e Ehrlichia canis, pelo ensaio imunoenzimático indireto e pela reação de imunofluorescência indireta

To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.

Avaliação da reatividade cruzada entre antígenos de Leishmania spp e Trypanosoma cruzi na resposta sorológica de cães pela técnica de imunofluorescência indireta (RIFI)

Visceral Leishmaniasis (VL) is a zoonosis caused by Leishmania spp. protozoa. Dog is the main parasite's reservoir, especially in urban areas. Indirect Immunofluorescent Antibody Test (IFAT) is commonly used for canine visceral leishmaniasis (CVL) diagnosis, by detection of antibodies against the parasite. However, Trypanosoma cruzi (T.cruzi) cross reactions can occur in serological diagnosis. In order to evaluate the occurrence of cross reactions between antigens of Leishmania spp. and T.cruzi, 150 blood samples of CVL epidemiological inquiry positive dogs were collected and sera were tested by IFAT. Dogs were culled at Zoonosis Control Center of Bauru (SP), an endemic area for VL. Another 150 dogs' blood samples were collected in Botucatu (SP), a non endemic area for LV, and sera were also tested by IFAT. Serum samples of dogs from Bauru were positive, both for Leishmania spp. and for T. cruzi, showing high cross-reactions incidence, reinforcing the need of diagnosis confirmation by other tests. Serum samples of dogs from Botucatu demonstrated low prevalence of positive results by IFAT, both for Leishmania spp. and T.cruzi. However, despite the low number of positive serological results for these parasites, the epidemiological and serological investigation for LV and Chagas disease in dogs from Botucatu must be kept, considering the importance of dog as domestic reservoir for both parasites.

Eletromiografia dos músculos temporal e masseter em crianças com mordida cruzada posterior direita

This study proposal was to evaluate the changes at a masticatory muscle temporalis and masseter, through a eletromyographic, at ponters of the right unilateral bite posterior, verifying the difference at the eletric activity between right and left sides. Twenty female volunteers, with ages between 7 and 8 (x = 7.4). The eletromyographics signals were collected in both sides in all volunteers in rest situations and in an usual isotonic mastication, right, left and bilateral and the maximun isometric constriction, which sinal was used to the eletric activity amplitude normalization. The results signed that the right masseter muscle presents an expressive statistic difference, if compared with the left masseter muscle during the left and right mastication, suggesting a right unilateral mastication. The muscle haven't gotten any expressive variance in this amplitude which remained to a proximate flat noticed in a right and habitual mastication, suggesting a right and one sided mastication during the left mastication occurs a raise of the eletric signal amplitude of the muscle left masseter. The right masseter muscle haven't gained any expressive variance, which remained in a proximate flat observed in an usual and right mastication, suggesting a muscular pattern hyperfunction next to the dental crossing.

Contaminação microbiana das soluções de processamento radiográfico: Risco de infecção cruzada

Objective: To evaluate the microbial contamination in samples of radiographic developing and fixing solutions, radiographic processing water, dental unit waterlines and dental office's external water supply. Methods: Samples of 50 private dental offices and 20 dental offices of the School of Dentistry de Araçatuba-UNESP were collected. The samples were subjected to neutralization of the residual inhibitory agents followed by pre-enrichment in peptone water and EVA broth and were inoculated in selective and non-selective culture media. The DNA of the samples was extracted and the presence of superinfectant microorganisms was evaluated by PCR. The differences in the prevalence of the microorganisms in the samples of the solutions were analyzed by repeated-measures analysis of variance for category data, while the Chi-Square and Mann-Whitney tests were used to evaluate the existence of associations between the total number of heterotrophic microorganisms and infection control protocols. The significance level was set at 5%. Results: Samples of radiographic fixing solution presented a remarkably less frequent contamination than radiographic developing solution and radiographic processing water. The latter exhibited microorganisms typically originated from the oral microbiota and human skin as well as microorganisms frequently found in dental unit waterlines. There was less contamination of radiographic processing water in the dental offices where the x-ray film packets were disinfected before processing or were covered by a plastic film. Additionally, all samples of dental office's external water supply were drinkable. Conclusion: Covering the x-ray film packets with a plastic film protection barrier was proven the best manner to reduce the contamination of the evaluated solutions.

Prevenção da contaminação cruzada em odontologia por meio de terapia fotodinâmica (TFD) utilizando LED azul e curcumina

Pós-graduação em Odontologia - FOAR

Análise de rótulos e bulas de agrotóxicos segundo dados exigidos pela legislação federal de agrotóxicos e afins e de acordo com parâmetros de legibilidade tipográfica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Contaminação cruzada em escovas dentais por Streptococcus mutans

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise dos resultados do tratamento da mordida cruzada posterior funcional com o expansor fixo Hyrax

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo do comportamento eletromiográfico do masseter e temporal após o tratamento da mordida cruzada posterior funcional com o aparelho quadrihélice

Pós-graduação em Odontologia - FOA

Avaliação dos efeitos dento-esqueléticos ocorridos após o tratamento da mordida cruzada posterior, com o uso do aparelho expansor fixo tipo quadrihélice

Pós-graduação em Odontologia - FOA