997 resultados para Brown, William Montgomery, 1855-1937.


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Letter which gives an addition to the estimate for work done by John Brown for Dec. 1855. This is made out to S.D. Woodruff from Francis Lalor. The request is for money for extra ties, fence posts, stone and labour. The letter is slightly singed at the bottom. This does not affect the text, Jan. 3, 1856.

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Inscripción en la parte superior: "Tipos Populares de España"

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Although membrane tubules can be found extending from, and associated with, the Golgi complex of eukaryotic cells, their physiological function has remained unclear. To gain insight into the biological significance of membrane tubules, we have developed methods for selectively preventing their formation. We show here that a broad range of phospholipase A2 (PLA2) antagonists not only arrest membrane tubule–mediated events that occur late in the assembly of the Golgi complex but also perturb its normal steady-state tubulovesicular architecture by inducing a reversible fragmentation into separate “mini-stacks.” In addition, we show that these same compounds prevent the formation of membrane tubules from Golgi stacks in an in vitro reconstitution system. This in vitro assay was further used to demonstrate that the relevant PLA2 activity originates from the cytoplasm. Taken together, these results demonstrate that Golgi membrane tubules, sensitive to potent and selective PLA2 antagonists, mediate both late events in the reassembly of the Golgi complex and the dynamic maintenance of its steady-state architecture. In addition, they implicate a role for cytoplasmic PLA2 enzymes in mediating these membrane trafficking events.

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Evidence has been presented both for and against obligate retrograde movement of resident Golgi proteins through the endoplasmic reticulum (ER) during nocodazole-induced Golgi ministack formation. Here, we studied the nocodazole-induced formation of ministacks using phospholipase A2 (PLA2) antagonists, which have been shown previously to inhibit brefeldin A–stimulated Golgi-to-ER retrograde transport. Examination of clone 9 rat hepatocytes by immunofluorescence and immunoelectron microscopy revealed that a subset of PLA2 antagonists prevented nocodazole-induced ministack formation by inhibiting two different trafficking pathways for resident Golgi enzymes; at 25 μM, retrograde Golgi-to-ER transport was inhibited, whereas at 5 μM, Golgi-to-ER trafficking was permitted, but resident Golgi enzymes accumulated in the ER. Moreover, resident Golgi enzymes gradually redistributed from the juxtanuclear Golgi or Golgi ministacks to the ER in cells treated with these PLA2 antagonists alone. Not only was ER-to-Golgi transport of resident Golgi enzymes inhibited in cells treated with these PLA2 antagonists, but transport of the vesicular stomatitis virus G protein out of the ER was also prevented. These results support a model of obligate retrograde recycling of Golgi resident enzymes during nocodazole-induced ministack formation and provide additional evidence that resident Golgi enzymes slowly and constitutively cycle between the Golgi and ER.

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Left to right: Res. of Wm. P. Brown, Sec. 6, Ann Arbor, Mich.; J.G. Miller, Manufacturer of sash, doors, blinds, mouldings, scroll work &c. ... Ann Arbor, Mich.; Ann Arbor Steam Planing Mill , Bracket, Scroll & Fancy Sawing, cor. of North & Fifth Sts., Ann Arbor, Mich. (na8635); Marble Works & Res. of Anton Eisele, the largest marble works in Washtenaw Co. Mich.; City Market, G.F. Gwinner, cor. of Fourth & Washington Sts., Ann Arbor, Mich.; L.C. Risdon, 31 South Main St., Ann Arbor, Mich. (na8635; na6707). Publication information: Chicago, Ill. : Everts & Stewart, 1874.

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Back row-standing: Keene Fitzpatrick, Joe Maddock, Coach Fielding Yost, Dan McGugin, Eugene Tefler, Frank Doty, Rolla Bigelow, Lorin(?) Jones, Andrews, Andrews, Samuel Sackett, DeStelle DeLappe, Anderson, William Snushall, Elmer Shank, Brown, William Cole, Clarence Wilcox, Shirk, Lake, James Turner, Kennedy Potter, George Read, Richardson, Ralph Drake, John Hincks, Clifford Kennedy

Middle Row-kneeling: Chauncey Brewer, John H. James, David Smith, Abner Howell, James Forrest, William Palmer, George Edmonds, Edwards, Spaulding, John Belford, Davis, Eldred Keays, Samuel Ball, Hemeneway, Palmer,

Front Row: Charles VanValkenberg, Paul Dickey, Herbert Graver, Paul Jones, Everett Sweeley, Charles Carter, Albert Herrnstein, Ross Kidston, George Gregory, William Weeks, James Knight, Norman Sterry, Curtis Redden, Harold Baker, Moses Johnson, James Maynard, Frederick Woodward, Chris Cron, Chester Apel, Cooley, John Lewis

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Bibliography: p. 124-128.

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Bibliography: p. 79-82.

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Mode of access: Internet.

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"Published April, 1935. Reprinted ... May, 1940."