Propagação de Hancornia speciosa Gomes – Apocynaceae, por alporquia e micropropagação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reguladores vegetais e bioestimulantes no desenvolvimento de Salvia officinalis L: avaliações fisológicas, bioquímicas e fitoquímicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produtos de efeitos fisiológicos no desenvolvimento de plantas de tomate ‘giuliana’, na produção e pós-colheita de frutos

Pós-graduação em Agronomia (Horticultura) - FCA

Morfogênese in vitro de nim a partir de explantes cotiledonares

Azadirachta indica A. Juss, popularmente conhecido como nim, é uma espécie arbórea que se destaca por possuir substâncias de ação inseticida, fungicida, bactericida e nematicida. Sementes de nim foram inoculadas em meio de cultura WPM (Wood Plant Medium) contendo diferentes concentrações de ácido giberélico (GA₃) (0; 3,0; 6,0; 9,0; e 12,0 mg L^-1). Após 30 dias, cotilédones obtidos a partir de plântulas germinadas in vitro foram inoculados em meio WPM suplementado com ácido 2,4-diclorofenoxiacético (2,4-D) (0,0; 1,0; 2,0; e 3,0 mg L^-1) e, ou, 6-benzilaminopurina (BAP) (0,0; 1,0; 2,0; e 3,0 mg L^-1). As culturas foram incubadas no escuro, a 28 ºC. Os calos formados foram avaliados com base na sua coloração e textura, e três subcultivos foram realizados mensalmente em meio WPM contendo 2,0 mg L^-1 BAP, na presença de luz. A cada 30 dias, avaliou-se o número de brotos formados a partir dos calos subcultivados. Entre os meios testados, o mais apropriado para germinação in vitro de nim foi o WPM na ausência de GA₃. Explantes cotiledonares cultivados em WPM suplementado com 2,0 mg L^-1 de BAP promoveram a maior formação de calos com potencial morfogênico. Quando esses calos foram transferidos para meio de composição similar, obteve-se alta formação de brotações até o terceiro subcultivo.

Protocolo para micropropagação de bananeira ‘Thap maeo’

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Métodos de propagação do pinhão manso (Jatropha curcas L.)

Pós-graduação em Agronomia - FEIS

Estudo da fisiologia de gemas laterais de cana-de-açucar durante o armazenamento

Pós-graduação em Ciências Biológicas (Botânica) - IBB

Adventitious shoot induction from leaf segments in Anthurium andreanum is affected by age of explant, leaf orientation and plant growth regulator

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade de frutos de tomate ‘giuliana’ tratados com produtos de efeitos fisiológicos

The present work evaluated the effect of strobilurins, boscalid, plant growth regulators and vegetal extracts on the physico-chemical quality of tomato fruits (Solanum lycopersicum L.), hybrid Giuliana. The fruits from each treatment were selected and separated in 4 repetitions: control, pyraclostrobin, boscalid, pyraclostrobin + boscalid, IBA + GA(3) + kinetin, GA(4+7) + benzylaminopurine and vegetal extract. The first application was carried out at 30 days after transplant and the following at every 15 days. The evaluations were: weight loss, titratable acidity (TA), soluble solids (SS), SS/TA relation, pH, ascorbic acid content, texture, total soluble sugars (TSS), activity of pectin methyl esterase (PME) and polygalacturonase (PG). The SS content, responsible for fruit flavor, varied accordingly to the treatment, being highest for GA(4+7) + benzylaminopurine and lowest for the control. The same occurred regarding the SS/AT relation. The fruits were kept on the shelf for 9 days, at room temperature, being that at the end of this period several treatments still presented fruit in optimal consumption conditions, notably the boscalid treatment, which presented lowest weight loss, followed by pyraclostrobin. Therefore, it can be concluded that the application of the treatments did not modify the values for pH, AT and AST of the fruits. The highest PME activity was observed for the treatments with boscalid and the mixture of boscalid and pyraclostrobin, while the lowest PG activity occurred in the control and the pyraclostrobin treatment, indicating that some products accelerated the process of demethylation of pectins by PME, facilitating the action of PG.

Acúmulo de carboidratos no desenvolvimento de tomateiro tratado com produtos químicos

This work had the purpose to study the physiological effects of pyraclostrobin, boscalid, plant growth regulators and plant extract on the accumulation of carbohydrates during the development of tomato plants (Solanum lycopersicum L.), hybrid Giuliana, in protected environment conditions. The treatments were: T1- control; T2- pyraclostrobin 0.2 g L-1; T3- boscalid 0.075 g L-1, T4- pyraclostrobin 0.2 g L-1 + boscalid 0.075 g L-1, T5- IBA + GA(3) + kinetin 375 mg L-1, T6- GA(4+7) + benzylaminopurine 100 mg L-1 and T7- plant extract 100 mg L-1. The carbohydrate accumulation curve was accomplished with 5 samples, at 20-day intervals between evaluations, the 1st evaluation being carried out at 30 days after transplantation, on the day of the first treatment application. At each sampling the plants were separated in stem, leaves and fruits, of which the contents of total soluble sugars, reducing sugars and saccharose were evaluated. The effects of the treatments on chlorophyll content and gas exchanges were also evaluated. The experimental design was completely randomized, with 4 repetitions and 6 destructive evaluations during the development, with 1 plant per experimental unit for each sampling. The pyraclostrobin and boscalid applied in isolation and/or combined favor the increase of carbohydrates in leaves, stems and fruits of tomato hybrid Giuliana.

Quality in anthurium andraeanum after postharvest BAP treatment

To ensure the ongoing quality of anthurium inflorescence it is necessary to use postharvest treatments aimed at extending the vase life and delaying senescence. Thus, the objective of this study was to assess the effect of postharvest spray application of 6-benzylaminopurine (BAP) in anthurium quality and sugar levels. Two experiments were done, one where inflorescences were sprayed 0, 100 and 200 mg L-1 of BAP and kept under 23±1°C and relative humidity of 65±5% for evaluations, and in the second experiment BAP concentrations were reduced to 0, 50 and 100 mg L-1 and inflorescences were cold stored for 2 days at 13±1°C and then transferred to the same evaluation conditions from the first experiment. Quality assessments were performed according to Paull (1982) for spathe loss of gloss and blueing and spadix necrosis along with stem weighting for fresh weight (FW) variation every two days. In the first experiment FW loss was significantly lower from the 14th until 26th day after harvest. Anthurium sprayed with 100 and 200 mg L-1 showed significantly less fresh weight loss when compared to control stems and in the second experiment 50 and 100 mg L-1 did not reduce fresh weight loss. Means of scores from quality analysis, blueing and loss of gloss of spathes and spadix necrosis, were significantly lower in BAP sprayed anthurium (100 and 200 mg L-1) than in control flowers and by lowering BAP spray concentration and cold storing stems for two days this positive effect was only observed for spadix necrosis and not observed for spathe parameters.

In vitro propagation of Sacha inchi

The aim of this study was to perform an in vitro evaluation of the auxin: cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia Volubilis Linneo) seeds germinated in vitro. The segments apical (A), median (B) and basal (C) were introduced into semi-solid MS culture medium (2.0g L-1 Phytagel), supplemented with MS vitamins, sucrose (30.0g L-1) and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L-1), associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L-1), totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment ( A) presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.

IN VITRO ORGANOGENESIS OF RANGPUR LIME

Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were obtained when epicotyl segments-derived explants were evaluated. Considering the explant responsiveness of hypocotyl segments-derived explants no difference was detected between the entire hypocotyl segment and the hypocotyl segment attached to a cotyledon fragment. Moreover, the percentage of responsive explants decreased when hypocotyl segments divided longitudinally were tested. No difference was detected for the number of shoots developed per explant considering the three types of hypocotyl-derived explants. Culture media supplementation with BAP was not essential for Rangpur lime in vitro organogenesis. However, adventitious shoot development was stimulated in concentrations between 0.5 - 1.0 mg L-1.

In vitro organogenesis of zucchini squash cv. Caserta

A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 mu M), under a 16-h photoperiod at 27 degrees C. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 mu M) and incubated under a 16-h photoperiod at 27 degrees C. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 mu M of BAP. After 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. These buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. The morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. The results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. Caserta. The protocol will be further examined for future use in genetic transformation experiments in this species.

In vitro organogenesis of rangpur lime

Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were obtained when epicotyl segments-derived explants were evaluated. Considering the explant responsiveness of hypocotyl segments-derived explants no difference was detected between the entire hypocotyl segment and the hypocotyl segment attached to a cotyledon fragment. Moreover, the percentage of responsive explants decreased when hypocotyl segments divided longitudinally were tested. No difference was detected for the number of shoots developed per explant considering the three types of hypocotyl-derived explants. Culture media supplementation with BAP was not essential for Rangpur lime in vitro organogenesis. However, adventitious shoot development was stimulated in concentrations between 0.5 - 1.0 mg L-1.