Les champignons (fungi, hyménomycètes) qui croissent en France. Description et iconographie propriétés utiles ou vénéneuses.

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Les champignons (fungi, hyménomycètes) qui croissent en France. Description et iconographie propriétés utiles ou vénéneuses.

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Les champignons (fungi, hyménomycètes) qui croissent en France. Description et iconographie propriétés utiles ou vénéneuses.

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Les champignons (fungi, hyménomycètes) qui croissent en France. Description et iconographie propriétés utiles ou vénéneuses.

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Characterization of the Aspergillus nidulans biotin biosynthetic gene cluster and use of the bioDA gene as a new transformation marker.

The genes involved in the biosynthesis of biotin were identified in the hyphal fungus Aspergillus nidulans through homology searches and complementation of Escherichia coli biotin-auxotrophic mutants. Whereas the 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase are encoded by distinct genes in bacteria and the yeast Saccharomyces cerevisiae, both activities are performed in A. nidulans by a single enzyme, encoded by the bifunctional gene bioDA. Such a bifunctional bioDA gene is a genetic feature common to numerous members of the ascomycete filamentous fungi and basidiomycetes, as well as in plants and oömycota. However, unlike in other eukaryota, the three bio genes contributing to the four enzymatic steps from pimeloyl-CoA to biotin are organized in a gene cluster in pezizomycotina. The A. nidulans auxotrophic mutants biA1, biA2 and biA3 were all found to have mutations in the 7,8-diaminopelargonic acid synthase domain of the bioDA gene. Although biotin auxotrophy is an inconvenient marker in classical genetic manipulations due to cross-feeding of biotin, transformation of the biA1 mutant with the bioDA gene from either A. nidulans or Aspergillus fumigatus led to the recovery of well-defined biotin-prototrophic colonies. The usefulness of bioDA gene as a novel and robust transformation marker was demonstrated in co-transformation experiments with a green fluorescent protein reporter, and in the efficient deletion of the laccase (yA) gene via homologous recombination in a mutant lacking non-homologous end-joining activity.

What uses are mating types? The "developmental switch" model.

Why mating types exist at all is subject to much debate. Among hypotheses, mating types evolved to control organelle transmission during sexual reproduction, or to prevent inbreeding or same-clone mating. Here I review data from a diversity of taxa (including ciliates, algae, slime molds, ascomycetes, and basidiomycetes) to show that the structure and function of mating types run counter the above hypotheses. I argue instead for a key role in triggering developmental switches. Genomes must fulfill a diversity of alternative programs along the sexual cycle. As a haploid gametophyte, an individual may grow vegetatively (through haploid mitoses), or initiate gametogenesis and mating. As a diploid sporophyte, similarly, it may grow vegetatively (through diploid mitoses) or initiate meiosis and sporulation. Only diploid sporophytes (and not haploid gametophytes) should switch on the meiotic program. Similarly, only haploid gametophytes (not sporophytes) should switch on gametogenesis and mating. And they should only do so when other gametophytes are ready to do the same in the neighborhood. As argued here, mating types have evolved primarily to switch on the right program at the right moment.

Els fongs de Catalunya occidental segons les prospeccions recents. II

This paper contains the conclusion of a mycological survey of western Catalonia. The first part of the results was published in Acta Bot. Barc. 45 (Homenatge a Oriol de Bolòs): 57-89. The present part covers the last two groups of Basidiomycota: the agarics (326 species) and the gasteromycetes (44 species). The data regarding the surveyed area, collection localities, abbreviations used in the information on ecology, collectors and identifiers are found in the mentioned first part, which contains records of 37 species of Myxomycota, 5 of Zygomycota, 101 of mitosporic fungi, 8 of Teliomycetes, 1 of Ustomycetes, 16 of Phragmobasidiomycetes and 92 of Aphyllophorales. Together with the first part, the results of our survey are a useful contribution to an improved understanding of the fungal component of plant communities of the dry, warm lowlands of the western Mediterranean region, and highlight the remarkable reproductive activity of the fungi observed during late Autumn and Winter, in the studied area.

Spore germination and development of young mycelia in some Rhizopogon species

Spore germination in Rhizopogon abietis, R. luteolus, R. roseolus and R. villosulus was induced in the presence of Rhodotorula glutinis and activated charcoal, in agar medium (N6:5). In one R. roseolus sample, 51% of spores germinated within 35 days, allowing observation of the course of spore germination and the different developmental patterns of homokaryotic mycelia. In these plates, spores showed two times of germination. The spores that germinated early produced an apical germ tube. Later other spores germinated in proximity to young mycelium, by forming a germ vesicle. One of the hyphal growth patterns obtained (interruption-swelling-ramification) is similar to that reported for other fungi. With this technique for inducing spore germination, it is possible to obtain enough monosporic cultures to perform mating tests. Key words: Basidiomycotina, Hypogeous, Monosporic Cultures.

Collections in Institutional Herbaria listed as Rhizopogon, but not belonging to this genus

Fifty-five collections misidentified and stored in various institutional herbaria, under severa1 Rhizopogon species narnes, are revised. Among these, twelve belong to Ascomycotina and 9 were identified to species level; the other collections, immature fruitbodies wcre impossible to identify with the data available. Forty-two collections are Basidiomycotina and only an immature Scleroderma remain without identification to the species level.

Aportacion al conocimiento de los hongos del SE de España. IX. Uredinales, Ustilaginales, Tremelales, Auriculariales, Septobasidiales, Dacrimicetales (Basidiomicetes)

Se da cuenta del hallazgo, en el SE de España de 28 especies, pertenecientes a: Uredinales (15, entre las cuales: Gymnosporangium confusum Plow., G. fuscum D. C., G. gracile P a t., Puccinia atropae Mont., P. barbeyi (Roum.) Magn., P. tumida Grev., Uromyces monspessulanus Tranzsch.), Ustilaginales (4), Tremelales (4, con Exidiopsis grisea (Pers.) Bdot. y Maire, E laccata (Bdot. y Galz.) Luck-Allen), Auriculariales (1), Septobasidiales (Septobasidium mariani Bres.) y Dacrimicetales (3, con Calocera palmata (Schum.) F r.).

Evolution of uni- and bifactorial sexual compatibility systems in fungi.

Mating systems, that is, whether organisms give rise to progeny by selfing, inbreeding or outcrossing, strongly affect important ecological and evolutionary processes. Large variations in mating systems exist in fungi, allowing the study of their origin and consequences. In fungi, sexual incompatibility is determined by molecular recognition mechanisms, controlled by a single mating-type locus in most unifactorial fungi. In Basidiomycete fungi, however, which include rusts, smuts and mushrooms, a system has evolved in which incompatibility is controlled by two unlinked loci. This bifactorial system probably evolved from a unifactorial system. Multiple independent transitions back to a unifactorial system occurred. It is still unclear what force drove evolution and maintenance of these contrasting inheritance patterns that determine mating compatibility. Here, we give an overview of the evolutionary factors that might have driven the evolution of bifactoriality from a unifactorial system and the transitions back to unifactoriality. Bifactoriality most likely evolved for selfing avoidance. Subsequently, multiallelism at mating-type loci evolved through negative frequency-dependent selection by increasing the chance to find a compatible mate. Unifactoriality then evolved back in some species, possibly because either selfing was favoured or for increasing the chance to find a compatible mate in species with few alleles. Owing to the existence of closely related unifactorial and bifactorial species and the increasing knowledge of the genetic systems of the different mechanisms, Basidiomycetes provide an excellent model for studying the different forces that shape breeding systems.

Macromicets de l'illa de Menorca.II.

Donem a conèixer els resultats de les prospeccions micològiques realitzades a Menorca entre els anys 1980 i 1988, i els presentem en forma de catàleg. S'han estudiat 193 tàxons (10 Ascomycetes i 183 Basidiomycetes), dels quals 127 creiem corresponen a citacions noves per a l'illa, entre els quals destaquem: Agaricus pequinii (Boud.) Konr. & Maubl., Amanita boudieri Barla, Dermoloma pseudocuneifolium Henrink, Hebeloma sarcophyllum (Peck) Sacc., Inocybe tenebrosa Quél., Psathyrella ammophila (Dur. & Lev.) Orton, Lactarius cistophilus Bon & Trimb. Russula seperina Dupain i Battarraea phalloides (Dicks.) Pers

Contribució a la flora dels macromicets de l'illa de Mallorca

Com a resultat de les prospeccions micològiques realitzades a Mallorca entre els anys 1983 i 1990, donem a conèixer un catàleg de 218 taxons (8 Ascomycetes i 210 Basidiomycetes), dels quals creiem que 74 corresponen a citacions noves per a l'illa. En destaquem, entre d'altres: Cordyceps militaris (L.) Link, Daldinia vernicosa (Schw.) Ces. et de Not., Dichomitus campestris (Quél.) Domanski et Ori., Agaricus lanipes (Moell. et Schaeff.) Sing., Amanita boudieri Barla, Clitocybe lituus (Fr.) Metr., Hygrocybe reai Mre., Inocybe tenebrosa Quél., Leucopaxillus tricolor (Peck) Kühn, i Russula seperina Dupain.

Characterization of the surface hydrophobicity of filamentous fungi.

A method for the quantitative analysis of the hydrophobicity of the mycelial mat of filamentous fungi based on contact angle measurements is presented. It was tested for a range of fungi belonging to the classes of basidiomycetes, ascomycetes and deuteromycetes. The measured contact angles of the mycelial mats ranged between hydrophilic (<30 degrees) for the deuteromycetes Fusarium oxysporum Fo47 GUS1 and Trichoderma harzianum P1[pZEGA1] and hydrophobic (>60 degrees) for the ascomycete Cladosporium sp. DSE48.1b and the basidiomycetes Paxillus involutus WSL 37.7, Hebeloma crustiliniforme WSL 6.2, Suillus bovinus WSL 48.1 and Laccaria bicolor WSL 73.1. For some fungi, variations in the hydrophobicity of the mycelium depending on the growth medium, the physiological state and the exposure to water were distinguished.

Contributions of the peroxisome and β-oxidation cycle to biotin synthesis in fungi.

The first step in the synthesis of the bicyclic rings of D-biotin is mediated by 8-amino-7-oxononanoate (AON) synthase, which catalyzes the decarboxylative condensation of l-alanine and pimelate thioester. We found that the Aspergillus nidulans AON synthase, encoded by the bioF gene, is a peroxisomal enzyme with a type 1 peroxisomal targeting sequence (PTS1). Localization of AON to the peroxisome was essential for biotin synthesis because expression of a cytosolic AON variant or deletion of pexE, encoding the PTS1 receptor, rendered A. nidulans a biotin auxotroph. AON synthases with PTS1 are found throughout the fungal kingdom, in ascomycetes, basidiomycetes, and members of basal fungal lineages but not in representatives of the Saccharomyces species complex, including Saccharomyces cerevisiae. A. nidulans mutants defective in the peroxisomal acyl-CoA oxidase AoxA or the multifunctional protein FoxA showed a strong decrease in colonial growth rate in biotin-deficient medium, whereas partial growth recovery occurred with pimelic acid supplementation. These results indicate that pimeloyl-CoA is the in vivo substrate of AON synthase and that it is generated in the peroxisome via the β-oxidation cycle in A. nidulans and probably in a broad range of fungi. However, the β-oxidation cycle is not essential for biotin synthesis in S. cerevisiae or Escherichia coli. These results suggest that alternative pathways for synthesis of the pimelate intermediate exist in bacteria and eukaryotes and that Saccharomyces species use a pathway different from that used by the majority of fungi.