952 resultados para Adiponectine récepteurs 1 et 2


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Mode of access: Internet.

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Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.

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Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

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Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.

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Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

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Mixed ligand complexes of the type Ni(R-AB)(AC') and Ni(R-AC)(AB') where AB/AC denote N-bonded isonitroso- [3-ketoimino ligands, AB'/AC' denote the corresponding Obonded ligands and R = Me, Et, n-Pr are synthesised and characterised. The complexes are neutral with square planar geometry around nickel(II). The bonding isomerism of the isonitroso group is discussed on the basis of i.r. and 1H n.m.r. studies. The crystal structure of the title complex, Ni(n-Pr-IEAI)(IMAI') has been determined from diffractometer data by Patterson and Fourier methods and refined by least squares to R = 0.088 for 2209 observed reflections. Unit cell constants are: a = 11.945(2), b = 22.436(7), c = 13.248(5) ~, [3 = 95.13(2) ~ The space group is P2Jc with Z = 8. Niekel(II) has a square planar coordination of two imine nitrogens, an isonitroso-nitrogen (from n-Pr-IEAI) and another isonitrosooxygen (from IMAI').

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Some tetra substituted furans and thiophenes were reacted with methyl acrylate under BF3-etherate catalysed Diels-Alder conditions. While the derivatives of furan underwent Diels-Alder reaction in a facile manner, an observation of 2,5-dimethyl-3,4-dianisylthiophene undergoing Diels-Alder reaction with methyl acrylate is remarkable. (C) 1997, Elsevier Science Ltd.

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The anisotropy of 1.3 - 2.3 MeV protons in interplanetary space has been measured using the Caltech Electron/Isotope Spectrometer aboard IMP-7 for 317 6-hour periods from 72/273 to 74/2. Periods dominated by prompt solar particle events are not included. The convective and diffusive anisotropies are determined from the observed anisotropy using concurrent solar wind speed measurements and observed energy spectra. The diffusive flow of particles is found to be typically toward the sun, indicating a positive radial gradient in the particle density. This anisotropy is inconsistent with previously proposed sources of low-energy proton increases seen at 1 AU which involve continual solar acceleration.

The typical properties of this new component of low-energy cosmic rays have been determine d for this period which is near solar minimum. The particles have a median intensity of 0.06 protons/ cm^(2)-sec-sr-MeV and a mean spectral index of -3.15.The amplitude of the diffusive anisotropy is approximately proportional to the solar wind speed. The rate at which particles are diffusing toward the sun is larger than the rate at which the solar wind is convecting the particles away from the sun. The 20 to 1 proton to alpha ratio typical of this new component has been reported by Mewaldt, et al. (1975b).

A propagation model with κ_(rr) assumed independent of radius and energy is used to show that the anisotropy could be due to increases similar to those found by McDonald, et al. (1975) at ~3 AU. The interplanetary Fermi-acceleration model proposed by Fisk (1976) to explain the increases seen near 3 AU is not consistent with the ~12 per cent diffusive anisotropy found.

The dependence of the diffusive anisotropy on various parameters is shown. A strong dependence of the direction of the diffusive anisotropy on the concurrently measured magnetic field direction is found, indicating a κ_⊥ less than κ_∥ to be typical for this large data set.

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根癌农杆菌通过将一段含有“癌”基因的T-DNA导入植物基因组中,引起植物的肿瘤:冠瘿。根癌农杆菌的这种能力来源于Ti质粒(Tumor inducing plasmid)。遗传工程中,根癌农杆菌的这一特性被用来将连接入Ti质粒T-DNA区两个边界之间的外源基因转入植物基因组。随着植物分子生物学的发展,T-DNA转化的原理被进一步阐明,农杆菌介导的转基因技术也得到进一步优化,更适合遗传工程操作。特别是Ti质粒毒性区和T-DNA区的反式作用(即位于不同质粒的T-DNA和毒性区也能侵染植物)被发现以来,双元表达载体的构建使遗传工程操作大为简便。 常用的双元表达载体大小都在11kb以上,尽管远远小于几百kb的野生型Ti质粒,但在实际的体外操作中还是不够简便。常用的植物双元表达载体pBI121的基因序列被测定(Frisch et al.,1995),数据显示非T-DNA区一半以上的序列被发现和功能无关,这使双元载体的进一步缩小成为可能。本文即通过PCR方法克隆到pBI121非T-DNA区中载体复制、三亲杂交必需的片段,结合载体pART27中的T-DNA区(含有真核、原核表达活性的嵌合npt II基因)创造了小的合成型植物表达双元载体pSY1(小于7kb)。然后将pBI121上带有35S启动子和nos终止子的GUS基因克隆到pSY1的T-DNA区中,得到pSY2(约10kb)。进一步用pROK2上的35S启动子和nos终止子区替换pSY2上的GUS表达区,得到pSY3(约8kb)。通过三亲法将pSY2转入根癌农杆菌中,根癌农杆菌再通过叶盘法侵染烟草叶片,获得愈伤组织,愈伤组织进一步分化出小苗。GUS组织化学染色表明GUS基因在转基因的愈伤组织和小苗中均有表达,PCR检测也证明GUS基因被导入了植物基因组。pSY系列载体能成功的用于植物遗传转化。

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Comprend : Observatio de nummis romanis in quibus S. C. et EX S. C. notatum legitur