High-Throughput Prediction of Acacia and Eucalypt Lignin Syringyl/Guaiacyl Content Using FT-Raman Spectroscopy and Partial Least Squares Modeling

High-throughput techniques are necessary to efficiently screen potential lignocellulosic feedstocks for the production of renewable fuels, chemicals, and bio-based materials, thereby reducing experimental time and expense while supplanting tedious, destructive methods. The ratio of lignin syringyl (S) to guaiacyl (G) monomers has been routinely quantified as a way to probe biomass recalcitrance. Mid-infrared and Raman spectroscopy have been demonstrated to produce robust partial least squares models for the prediction of lignin S/G ratios in a diverse group of Acacia and eucalypt trees. The most accurate Raman model has now been used to predict the S/G ratio from 269 unknown Acacia and eucalypt feedstocks. This study demonstrates the application of a partial least squares model composed of Raman spectral data and lignin S/G ratios measured using pyrolysis/molecular beam mass spectrometry (pyMBMS) for the prediction of S/G ratios in an unknown data set. The predicted S/G ratios calculated by the model were averaged according to plant species, and the means were not found to differ from the pyMBMS ratios when evaluating the mean values of each method within the 95 % confidence interval. Pairwise comparisons within each data set were employed to assess statistical differences between each biomass species. While some pairwise appraisals failed to differentiate between species, Acacias, in both data sets, clearly display significant differences in their S/G composition which distinguish them from eucalypts. This research shows the power of using Raman spectroscopy to supplant tedious, destructive methods for the evaluation of the lignin S/G ratio of diverse plant biomass materials.

Life cycle and host range of Phycitasp. rejected for biological control of prickly acacia in Australia

Prickly acacia (Vachellia nilotica subsp. indica), a native of the Indian subcontinent, is a serious weed of the grazing areas of northern Australia and is a target for classical biological control. Native range surveys in India identified a leaf webber, Phycita sp. (Lepidoptera: Pyralidae) as a prospective biological control agent for prickly acacia. In this study, we report the life cycle and host-specificity test results Phycita sp. and highlight the contradictory results between the no-choice tests in India and Australia and the field host range in India. In no-choice tests in India and Australia, Phycita sp. completed development on two of 11 and 16 of 27 non-target test plant species, respectively. Although Phycita sp. fed and completed development on two non-target test plant species (Vachellia planifrons and V. leucophloea) in no-choice tests in India, there was no evidence of the insect on the two non-target test plant species in the field. Our contention is that oviposition behaviour could be the key mechanism in host selection of Phycita sp., resulting in its incidence only on prickly acacia in India. This is supported by paired oviposition choice tests involving three test plant species (Acacia baileyana, A. mearnsii and A. deanei) in quarantine in Australia, where eggs were laid only on prickly acacia. However, in paired oviposition choice trials, only few eggs were laid, making the results unreliable. Although oviposition choice tests suggest that prickly acacia is the most preferred and natural host, difficulties in conducting choice oviposition tests with fully grown trees under quarantine conditions in Australia and the logistic difficulties of conducting open-field tests with fully grown native Australian plants in India have led to rejection of Phycita sp. as a potential biological control agent for prickly acacia in Australia.

The leaf-feeding geometrid Isturgia disputaria (Guenee)-A potential biological control agent for prickly acacia, Vachellia nilotica subsp. indica (Benth.) Kyal. & Boatwr. (Mimosaceae) in Australia

Prickly acacia (Vachellia nilotica subsp. indica), a native multipurpose tree in India, is a weed of National significance, and a target for biological control in Australia. Based on plant genetic and climatic similarities, native range surveys for identifying potential biological control agents for prickly acacia were conducted in India during 2008-2011. In the survey leaf-feeding geometrid, Isturgia disputaria Guenee (syn. Tephrina pulinda), widespread in Tamil Nadu and Karnataka States, was prioritized as a potential biological control agent based on field host range, damage potential and no choice test on non target plant species. Though the field host range study exhibited that V. nilotica ssp. indica and V. nilotica ssp. tomentosa were the primary hosts for successful development of the insect, I. disputaria, replicated no - choice larval feeding and development tests conducted on cut foliage and live plants of nine non-target acacia test plant species in India revealed the larval feeding and development on three of the nine non-target acacia species, V. tortilis, V. planiferons and V. leucophloea in addition to the V. nilotica ssp. indica and V. nilotica ssp. tomentosa. However, the proportion of larvae developing into adults was higher on V. nilotica subsp. indica and V. nilotica subsp. tomentosa, with 90% and 80% of the larvae completing development, respectively. In contrast, the larval mortality was higher on V. tortilis (70%), V. leucophloea (90%) and V. planiferons (70%). The no-choice test results support the earlier host specificity test results of I. disputaria from Pakistan, Kenya and under quarantine in Australia. Contrasting results between field host range and host use pattern under no-choice conditions are discussed.

A co-evolutionary relationship exists between Endoraecium (Pucciniales) and its Acacia hosts in Australia

Endoraecium is a genus of rust fungi that infects several species of Acacia in Australia, South-East Asia and Hawaii. This study investigated the systematics of Endoraecium from 55 specimens in Australia based on a combined morphological and molecular approach. Phylogenetic analyses were conducted on partitioned datasets of loci from ribosomal and mitochondrial DNA. The recovered molecular phylogeny supported a recently published taxonomy based on morphology and host range that divided Endoraecium digitatum into five species. Spore morphology is synapomorphic and there is evidence Endoraecium co-evolved with its Acacia hosts. The broad host ranges of E. digitatum, E. parvum, E. phyllodiorum and E. violae-faustiae are revised in light of this study, and nine new species of Endoraecium are described from Australia based on host taxonomy, morphology and phylogenetic concordance.

Carbon Dioxide emissions from an Acacia plantation on peatland in Sumatra, Indonesia

Abstract. Peat surface CO2 emission, groundwater table depth and peat temperature were monitored for two years along transects in an Acacia plantation on thick tropical peat (>4 m) in Sumatra, Indonesia. A total of 2300 emission measurements were taken at 144 locations. The autotrophic root respiration component of the CO2 emission was separated from heterotrophic emissions caused by peat oxidation in three ways: (i) by comparing CO2 emissions within and beyond the tree rooting zone, (ii) by comparing CO2 emissions with and without peat trenching (i.e. cutting any roots remaining in the peat beyond the tree rooting zone), and (iii) by comparing CO2 emissions before and after Acacia tree harvesting. On average, the contribution of root respiration to daytime CO2 emission is 21 % along transects in mature tree stands. At locations 0.5 m from trees this is up to 80 % of the total emissions, but it is negligible at locations more than 1.3 m away. This means that CO2 emission measurements well away from trees are free of any root respiration contribution and thus represent only peat oxidation emission. We find daytime mean annual CO2 emission from peat oxidation alone of 94 t ha−1 yr−1 at a mean water table depth of 0.8 m, and a minimum emission value of 80 t ha−1 yr−1 after correction for the effect of diurnal temperature fluctuations, which resulted in a 14.5 % reduction of the daytime emission. There is a positive correlation between mean long-term water table depths and peat oxidation CO2 emission. However, no such relation is found for instantaneous emission/water table depth within transects and it is clear that factors other than water table depth also affect peat oxidation and total CO2 emissions. The increase in the temperature of the surface peat due to plantation development may explain over 50 % of peat oxidation emissions.

Uso de vaina de espino negro (Acacia pennatula) como suplemento en alimentación de ganado lechero

Con el objetivo de determinar, el mejor nivel de inclusión de vaina de espino negro (Acacia Pennatula), en la dieta alimenticia de las vacas lecheras y su efecto sobre la producción de la leche, sus cambios físicos y químicos, así como los costos de producción, se realizó el estudio el en la hacienda San Francisco ubicada en la comarca de Pueblo Nuevo a 15 kilómetros al norte del municipio de Jinotega departamento de Jinotega a los 13° 11’ 59” Latitud N y 85° 56’ 34" Longitud W. Se utilizaron 15 animales, los cuales se separaron únicamente en la suplementación alimenticia, al momento del ordeño y el pesaje de leche, el resto del manejo fue el mismo para el resto del hato en dicha finca, se realizaron dos ordeños al día 1) de 5 a 6 a.m. y 2) de 3 a 4 p.m. Se utilizó un diseño cruzado (Cross over)de 3 x 3, con 3 bloques y 3 tratamientos (a) concentrado comercial, (b) concentrado con 40% de inclusión de vaina de espino negro y (c) concentrado con 60 % de inclusión de vaina de espino negro. Los datos fueron analizados utilizando el paquete estadístico SAS (1999), con procedimiento de separación de medias por Duncan. El modelo matemático fue: Yijkl m + Bi + Cj + Pk + T1 + Eijkl. Se suministró el alimento dos veces al día durante cada ordeño (5 de la mañana y 3 de la tarde), se oferto un total de 44 libras (20 kg.), de su respectivo tratamiento alimenticio de cada grupo, el cual fue distribuido al azar por medio de un sorteo con tres papelitos para cada grupo y para cada tratamiento. El proceso de adaptación inicio con 1 semana de aceptación y 3 semanas de toma de datos para cada periodo. Se encontró diferencias altamente significativas para los tratamientos, resultando el tratamiento "b" (concentrado con 40 % de vaina de espino negro), con los mejores resultados en cuanto a producción de leche (Con un promedio de 7.16 lt/vaca/ordeño) y porcentaje de grasa en la leche (ó.9 %). Un comportamiento intermedio lo obtuvo el tratamiento el tratamiento "a" (concentrado comercial), el cual obtuvo una producción de leche (promedio de 6.28 lt/vaca/ordeño) y un porcentaje de grasa de (5.6 %), con el más bajo resultado el tratamiento "c" (concentrado con 60% de inclusión de vaina de espino negro) que obtuvo un promedio de producción (5.08 lt/vaca/ordeño); con un porcentaje de grasa de (4.8 %), todos los casos se encontró cambios físicos, químicos no así organolépticos en la leche. Cuando se utilizó 40 % de inclusión de vaina de espino negro en la dieta del animal se encontró que los costos se reducían hasta en 50 % y ia producción aumenta hasta en un 20 %.

Envisioning 2050: climate change, aquaculture and fisheries in West Africa. Dakar, Senegal 14-16 April 2010

This report presents the activities and results of the workshop Envisioning 2050: Climate Change, Aquaculture and Fisheries in West Africa. The objectives of the workshop were to discuss critical issues and uncertainties faced by the fisheries and aquaculture sector in Ghana, Senegal and Mauritania, build sectoral scenarios for 2050 and discuss the implication of these scenarios in the context of climate change for the countries and the region.

Integrated farming: a new approach in the Basse Casamance, Senegal

Environmental changes due to 2 decades of drought have modified the ecosystem of the Basse Casamance, Senegal thus forcing farmers to change their practices. They have built fish ponds and diversified into crops such as peanut, millet, sorghum, cassava and vegetables. The ponds have 2 purposes - to protect ricefields against inflow of brackishwater and to raise fish. The fish complements rice, which is the main diet, adding animal protein and serving as a source of income. Although this integrated farming system is little developed at present, it has good potential to rationalize use of the resources available to farmers and to promote interactions between farm enterprises.

Fishpen culture as a new production system in dammed valleys in the mid-Casamance, Senegal

Fishpen culture is a possible means to increase fish production in Casamance, Senegal and to develop aquaculture without negative environmental effects. To study this possibility, a fishpen study was conducted in two dammed valleys, Guidir and Balobar in the area. Wild Sarotherodon melanotheron from 7.7 to 25.4 g and Tilapia guineensis from 7.7 to 35.0 g were stocked at densities ranging from 1.5 to 2.5 individuals/ super(m). Results are summarized in this article.