Validação de metodologia para análise de aminas bioativas em camarão utilizando cromatografia de íons

In this study, we worked with the validation of a methodology for analysis of bioactive amines in shrimp, considering it to be one of the main products of the northriograndense trade balance, maintaining the state of Rio Grande do Norte topped the list of Brazilian exports of this product the last decade. The sector of the Brazilian shrimp works exclusively with gray shrimp Litopenaeus Vannamei since the late 1990s. This study used liquid chromatography with conductimetric detector, using as the mobile phase methylsulfonic 3 mM acid (MSA) with gradient and phase C18 column with reverse the development of methodology for the analysis of bioactive amines in shrimp. In the sample preparation was used as 5% trichloroacetic acid (TCA) extraction solution. Validation analysis of biotativas amines (putrescine - PUT, histamine - HIST, agmatine - AGM, spermidine - EPD and spermine - EPN) in shrimp, the linear working range was 0.1 to 2.0 mg L-1 to was sensitive, homoscedastic, in effect, selective, accurate and precise array. Thus, considered feasible for these determinations bioactive amines in this array. Determined the concentration of these amines in fresh shrimps (AGM = 0.61 ± 0.05 mg kg- 1 EPD = 2.57 ± 0.14 mg kg-1 and EPN = 1.79 ± 0.11 mg kg-1), and freezing weather predetermined in cooked shrimp (AGM = 6.28 ± 0.18 mg kg-1, EPD = 12.72 ± 0.02 mg kg- 1 and EPN = 22.30 ± 0.60 mg kg-1), the shrimp with twenty-four hour stay at room temperature (PUT = 879.52 ± 28.12 mg kg-1, AGM = 848.13 ± 19.40 mg kg-1, ESPD = 13.59 ± 0.97 mg kg-1 and ESPN = 18.47 + 1.57 mg kg-1). In shrimp subjected to freezing for a week, two weeks, three weeks and four weeks, the results showed that there is an increase in the content of agmatine (7.31 ± 0.21 mg kg-1) while in spermine ( 1.22 ± 0.14 mg kg-1) and spermidine (below limit of quantification) there was a decrease in the freeze time, while there is a decrease in the level of spermidine not reaching detectad. The putrescine was only found in shrimp that remained for 24 hours at room temperature and histamine was not found in any of the samples

Efeito de parâmetros de extrusão na cor E propriedades de pasta da farinha de mandioquinha-salsa (Arracacia xanthorrhiza)

Processou-se neste trabalho a farinha de mandioquinha-salsa (Arracacia xanthorrhiza Bancr.) em uma linha de extrusão (mono rosca) variando as condições operacionais: umidade da farinha (11-19%), temperatura de extrusão (86-154ºC) e taxa de rotação da rosca (136-272rpm). Os parâmetros de cor analisados foram luminosidade (L*) e os componentes de cromaticidade a* e b*. Os parâmetros de propriedade de pasta analisados foram viscosidade inicial, pico de viscosidade, quebra de viscosidade, tendência a retrogradação e viscosidade final. Os resultados obtidos mostraram que a umidade da matéria-prima interferiu nos componentes de cor das farinhas com efeito significativo sobre a luminosidade e croma a*, e a temperatura interferiu no croma b* . Quanto ao efeito dos parâmetros de processo sobre as propriedades de pasta, a umidade interferiu nas viscosidades inicial e final dos produtos, pico e quebra de viscosidade, enquanto a temperatura de extrusão e a rotação da rosca tiveram influência sobre a tendência a retrogradação e viscosidade final dos produtos.

The light-cone gauge without prescriptions

Feynman integrals in the physical light-cone gauge are more difficult to solve than their covariant counterparts. The difficulty is associated with the presence of unphysical singularities due to the inherent residual gauge freedom in the intermediate boson propagators constrained within this gauge choice. In order to circumvent these non-physical singularities, the headlong approach has always been to call for mathematical devices - prescriptions - some successful and others not. A more elegant approach is to consider the propagator from its physical point of view, that is, an object obeying basic principles such as causality. Once this fact is realized and carefully taken into account, the crutch of prescriptions can be avoided altogether. An alternative, third approach, which for practical computations could dispense with prescriptions as well as avoiding the necessity of careful stepwise consideration of causality, would be of great advantage. and this third option is realizable within the context of negative dimensions, or as it has been coined, the negative dimensional integration method (NDIM).

Light-cone gauge integrals: prescriptionlessness at two loops

The only calculations performed beyond one-loop level in the light-cone gauge make use of the Mandelstam-Leibbrandt (ML) prescription in order to circumvent the notorious gauge dependent poles. Recently we have shown that in the context of negative dimensional integration method (NDIM) such prescription can be altogether abandoned, at least in one-loop order calculations. We extend our approach, now studying two-loop integrals pertaining to two-point functions. While previous works on the subject present only divergent parts for the integrals, we show that our prescriptionless method gives the same results for them, besides finite parts for arbitrary exponents of propagators. (C) 2000 Elsevier B.V. B.V. All rights reserved.

General massive one-loop off-shell three-point functions

In this work we compute the most general massive one-loop off-shell three-point vertex in D-dimensions, where the masses, external momenta and exponents of propagators are arbitrary. This follows our previous paper in which we have calculated several new hypergeometric series representations for massless and massive (with equal masses) scalar one-loop three-point functions, in the negative dimensional approach.

Massless and massive one-loop three-point functions in negative dimensional approach

In this article we present the complete massless and massive one-loop triangle diagram results using the negative dimensional integration method (NDIM). We consider the following cases: massless internal fields; one massive, two massive with the same mass m and three equal masses for the virtual particles. Our results are given in terms of hypergeometric and hypergeometric-type functions of the external momenta (and masses for the massive cases) where the propagators in the Feynman integrals are raised to arbitrary exponents and the dimension of the space-time is D. Our approach reproduces the known results; it produces other solutions as yet unknown in the literature as well. These new solutions occur naturally in the context of NDIM revealing a promising technique to solve Feynman integrals in quantum field theories.

One-loop n-point equivalence among negative-dimensional, Mellin-Barnes and Feynman parametrization approaches to Feynman integrals

We show that at one-loop order, negative-dimensional, Mellin-Barnes (MB) and Feynman parametrization (FP) approaches to Feynman loop integral calculations are equivalent. Starting with a generating functional, for two and then for n-point scalar integrals, we show how to reobtain MB results, using negative-dimensional and FP techniques. The n-point result is valid for different masses, arbitrary exponents of propagators and dimension.

Negative dimensional approach for scalar two-loop three-point and three-loop two-point integrals

The well-known D-dimensional Feynman integrals were shown, by Halliday and Ricotta, to be capable of undergoing analytic continuation into the domain of negative values for the dimension of space-time. Furthermore, this could be identified with Grassmannian integration in positive dimensions. From this possibility follows the concept of negative-dimensional integration for loop integrals in field theories. Using this technique, we evaluate three two-loop three-point scalar integrals, with five and six massless propagators, with specific external kinematic configurations (two legs on-shell), and four three-loop two-point scalar integrals. These results are given for arbitrary exponents of propagators and dimension, in Euclidean space, and the particular cases compared to results published in the literature.

Non-planar double-box, massive and massless pentabox Feynman integrals in the negative-dimensional approach

The negative-dimensional integration method is a technique which can be applied, with success, in usual covariant gauge calculations. We consider three two-loop diagrams: the scalar massless non-planar double-box with six propagators and the scalar pentabox in two cases, where six virtual particles have the same mass, and in the case all of them are massless. Our results are given in terms of hypergeometric functions of Mandelstam variables and also for arbitrary exponents of propagators and dimension D.

NDIM achievements: massive, arbitrary tensor rank and N-loop insertions in Feynman integrals

One of the main difficulties in studying quantum field theory, in the perturbative regime, is the calculation of D-dimensional Feynman integrals. In general, one introduces the so-called Feynman parameters and, associated with them, the cumbersome parametric integrals. Solving these integrals beyond the one-loop level can be a difficult task. The negative-dimensional integration method (NDIM) is a technique whereby such a problem is dramatically reduced. We present the calculation of two-loop integrals in three different cases: scalar ones with three different masses, massless with arbitrary tensor rank, with and N insertions of a two-loop diagram.

Negative-dimensional integration revisited

Feynman diagrams are the best tool we have to study perturbative quantum field theory. For this very reason the development of any new technique that allows us to compute Feynman integrals is welcome. By the middle of the 1980s, Halliday and Ricotta suggested the possibility of using negative-dimensional integrals to tackle the problem. The aim of this work is to revisit the technique as such and check on its possibilities. For this purpose, we take a box diagram integral contributing to the photon-photon scattering amplitude in quantum electrodynamics using the negative-dimensional integration method. Our approach enables us to quickly reproduce the known results as well as six other solutions as yet unknown in the literature. These six new solutions arise quite naturally in the context of negative-dimensional integration method, revealing a promising technique to handle Feynman integrals.

Crude Glycerin associated with starch or fiber-based energy ingredients at two levels of concentrate for beef cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)