Dietary crude protein requirement of Tilapia nilotica fry

To determine growth and survival of Tilapia nilotica fry fed formulated practical dry diets with varying crude protein levels, fish were subjected to 3 seperate feeding trials. Isocaloric practical diets containing 20, 25, 30 and 35% crude protein were fed to fry at 15% fish biomass daily for 7 weeks in 2 trials and another set containing 20, 25, 30, 35, 40, 45 and 50% was given for 8 weeks. On the basis of growth, survival and feed conversion, T. nilotica fry required 35% crude protein in the practical diets given at 15% fish biomass.

Yield isopleths of Tilapia esculenta Graham 1928 in Lake Victoria and Tilapia nilotica (Linnaeus) 1757 in Lake Albert

The yield equation given by BEVERTON and HOLT (1957) has several parameters which are difficult to estimate for tropical freshwater fish species. Nevertheless, some simplifying assumptions can be made and the most relevant parameters used to enable the construction of yield isopleths. Tilapia esculenfa has the following parameters: maximum length (L ∞=33.8 c.m. growth rate (K) = 0.32, natural mortality rate (M)=0.17 and the length at maturity (1 m)=22 cm. The optimum yield is obtained by catching the fish at a length of first capture of 26 em and a fishing mortality rate of 0.5. Tilapia nilotica with L ∞=49 cm, 1 m=36 cm, K=0.50 and M= 0.30 gives optimum yield when caught at a length of first capture of 35-36 cm with a fishing mortality rate of 0.5-0.6. The stuned Tilapia nilotica of Lake Albert has L ∞=17 cm, K=2.77,1 m=12 cm and M=3.37. With such a very high natural mortality, maximum yields would be obtained hy using a length of first capture less than 9 cm and a fishing mortality rate exceeding 1.8.

Preliminary observations on the feeding of Tilapia nilotica Linn. in Lake Rudolf

Tilapia nilotica is commercially very important throughout the Ethiopian region including the major rivers in West Africa, the Chad basin, the Nile and its associated lakes. The Tilapia fishery of Lake Rudolf is at present small, but potentially important, particularly on the eastern shores of the lake where fishing intensity is low. Preliminary results from observations on the feeding of Tilapia nilotica in Lake Rudolf are presented. The fish exhibit a regular diurnal feeding rhythm, commencing between 05.00 hours and 08.00 hours and ceasing between 14.00 hours and 18.00 hours. The largest fish appear to feed longer. Quantitative estimates of the daily food intake indicate less material to be ingested than by populations in other lakes. The lysis of algae, intestinal pH and food material are also investigated.

On the ecology and explorations of the fisheries of an East African rift valley lake, Part 1: On the bionomics and population structure of Tilapia nilotica Linnaeus 1757 in Lake Baringo, Kenya

An ecological survey of the fisheries of Lake Baringo, Kenya was carried out between August, 1972 and August, 1973. The bionomics and population structure of T. nilotica is described. Sampling was done with multifilament gillnets of graded mesh sizes from 51 mm to 178 mm in approximately 12.5 mm increments. The Lake was divided into three sampling and ecologically different zones - the south, central and north zones. The size range of T. nilotica of both sexes caught was between 5 and 27 cm (mode 16 cm) with a mean length of 16.07 cm. For all the collections, males dominated (55.3%) and a higher proportion of males were caught in January, August and November. The smallest mature male and female was 9 and 10 cm respectively. Males grow faster and mature at larger sizes than females. 50% of all males and females mature at 17.4 and 16:4 cm respectively. The periods of intense spawning were between August and October and January to April. The Tilapia were feeding best in central and north zones and the feeding intensity was reduced in January. Two endoparasites Contracaecum sp. and Clinostomum sp. were isolated from the Tilapia. The "condition" of the fish was better in the north than in the other two zones.

尼罗罗非鱼Tilapia nilotica卵巢发育过程中液晶态类脂滴组织化学初步研究

<正> 我们研究了尼罗罗非鱼卵巢中的液晶态。用偏光显微镜观察到在第3和第4时相卵母细胞的胞质、滤泡细胞和部份间质区存在着双折射和多种形式的“马尔它十字”。电子显微镜观察到这些“马尔它十字”小滴是多层的膜结构。根据这些小滴的性质和结构,将其归属于溶致性液晶(lyotropic liquid crystal)的片层相(lamellar phase),即纯净相(neat phase)(孙建民1981)。

Effects of several organic acids on the feeding behavior of Tilapia nilotica

Automatic recording of the frequency of feeding 'bites' was used to evaluate the effects of several organic acids (citric, metacectonic, lactic, acetic, and oxalic) on the stimulatory feeding behavior of Tilapia nilotica . Some of these acids are added to food stocks to retard spoilage. The results showed that citric acid at a concentration of 10(-2) to 10(-6) m, metacetonic acid at 10(-4) to 10(-6) m, and lactic acid at 10(-2) to 10(-5) m stimulated feeding. Fish tended to avoid metacetonic acid at 10(-3) m and acetic acid at 10(-3) m. Acetic acid at 10(-5) m and oxalic acid at 10(-6) m had no significant effects on fish feeding.

［（μ－CF���3CO＿2）＿2Ln（μ－CF���3HCO＿2）Al（i－Bu）＿2·2THFl＿2（Ln＝Nd，Y）配合物的NMR和X－射线衍射研究

对［μ－ＣＦ＿３ＣＯ＿２）＿２Ｌｎ（μ－ＣＦ＿３ＨＣＯ＿２）Ａｌ（ｉ－Ｂｕ）＿２·ＴＨＦｌ＿２（Ｌｎ＝Ｎｄ，Ｙ）配合物单晶结构的Ｘ－射线分析指出，配合物具有中心对称性，配位中心由两个稀土和两个Ａｌ离子组成，稀土由两个ＴＨＦ和６个ＴＦＡ分子配位形成畸变的三盖三棱柱结构，Ａｌ由两个ＴＦＡＧ和两个ｉ－Ｂｕ配位形成四面体结构。桥连Ａｌ与两个稀土的ＴＦＡ分子的羧基发生歧化加氢，其碳原子由ＳＰ￣２型转变为ＳＰ￣３型．ＮＭＲ研究表明，在ＴＨＦ溶液中，该配合物保持了它在单晶中的配位结构，所不同的是两个ｉ－Ｂｕ在溶液中有两种异构形成，二者间为慢交换过程。

Development of selective media for the isolation of yeasts and filamentous fungi from the sputum of adult patients with cystic fibrosis (CF)

Yeasts and filamentous fungi are beginning to emerge as significant microbial pathogens in patients with cystic fibrosis (CF), particularly in relation to allergic-type responses, as seen in patients with allergic bronchopulmonary aspergillosis (ABPA), Aspergillus bronchitis and in invasive fungal disease in lung transplant patients. Four fungal media were compared in this study, including Sabouraud Dextrose Agar (SDA) and Medium B, with and without the addition of selective antibiotics, where antibiotic-supplemented media were designated with (+). These media were compared for their ability to suppress contaminating, mainly Gram-ve pathogens, in CF sputa (Pseudomonas aeruginosa, Burkholderia cepacia complex [BCC] organisms) and to enhance the growth of fungi present in CF sputum. Medium B consisted of glucose (16.7 g/l), agar (20 g/l), yeast extract (30 g/l) and peptone (6.8 g/l) at pH 6.3 and both SDA(+) and Medium B+ were supplemented with cotrimethoxazole, 128 mg/l; chloramphenicol, 50 mg/l; ceftazidime, 32 mg/l; colistin, 24 mg/l). Employment of SDA(+) or Medium B+ allowed an increase in specificity in the detection of yeasts and moulds, by 42.8% and 39.3%, respectively, over SDA when used solely. SDA(+) had a greater ability than Medium B+ to suppress bacterial growth from predominantly Gram-ve co-colonisers. This is a significant benefit when attempting to detect and isolate fungi from the sputum of CF patients, as it largely suppressed any bacterial growth, with the exception of the BCC organisms, thus allowing for an increased opportunity to detect target fungal organisms in sputum and represented a significant improvement over the commercial medium (SDA), which is currently used. Overall, both novel selective media were superior in their ability to suppress bacteria in comparison with the commercially available SDA medium, which is routinely employed in most clinical microbiology diagnostic laboratories presently. Alternatively, Medium B+ had a great ability to grow fungi than SDA(+) and when employed together, the specificity of combined use was 82%, with a sensitivity for yeasts, filamentous fungi, and combined overall fungi of 96.0%, 92.3% and 96.0%, respectively. Overall, when employing one fungal selective medium for the routine detection of yeasts and filamentous fungi in the sputum of CF patients, we would recommend employment of Medium B+. However, we would recommend the combined employment of SDA(+) and Medium B+, in order to synergistically isolate and detect the greatest number of fungi present in CF sputa. (C) 2008 European Cystic Fibrosis Society. Published by Elsevier B.V All rights reserved.

Copying letters to patients with cystic fibrosis (CF): Letter content and patient perceptions of benefit

Background: Copying letters involves generating an extra copy of all correspondence between healthcare professionals about the patient, to the patient.

Development of a novel PCR assay for the identification of the black yeast, Exophiala (Wangiella) dermatitidis from adult patients with cystic fibrosis (CF)

Cystic fibrosis (CF) patients may suffer increased morbidity and mortality through colonisation, allergy and invasive infection from fungi. The black yeast, Exophiala dermatitidis (synonym Wangiella dermatitidis) has been found with increasing frequency in sputum specimens of CF patients, with reported isolation rates ranging from 1.1 to 15.7%. At present, no diagnostic PCR exists to aid with the clinical laboratory detection and identification of this organism. A novel species-specific PCR-based assay was developed for the detection of E. dermatitidis, based on employment of rDNA operons and interspacer (ITS) regions between these rDNA operons. Two novel primers, (designated ExdF &amp; ExdR) were designed in silico with the aid of computer-aided alignment software and with the alignment of multiple species of Exophiala, as well as with other commonly described yeasts and filamentous fungi within CF sputum, including Candida. Aspergillus and Scedosporium. An amplicon of approximately 455 by was generated, spanning the partial ITS I region - the complete 5.8S rDNA region - partial ITS2 region, employing ExdF (forward primer [16-mer], 5'-CCG CCT ATT CAG GTC C-3' and ExdR (reverse primer [16-mer], 5'-TCT CTC CCA CTC CCG C-3', was employed and optimised on extracted genomic DNA from a well characterised culture of E. dermatitidis, as well as with high quality genomic DNA template from a further 16 unrelated fungi, including Candida albicans, C. dubliniensis, C. parapsilosis, C. glabrata, Scedosporium apiospermum, Penicillium sp., Aspergillus fumigatus, Aspergillus versicolor, Pichia guilliermondii, Rhodotorula sp., Trichosporon sp., Aureobasidium pullulans, Fusarium sp., Mucor hiemalis, Bionectria ochroleuca, Gibberella pulicaris. Results demonstrated that only DNA from E. dermatitidis gave an amplification product of the expected sire, whilst none of the other fungi were amplifiable. Subsequent employment of this primer pair detected this yeast from mycological cultures from 2/50 (4%) adult CF patients. These two patients were the only patients who were previously shown to have a cultural history of E. dermatitidis from their sputum. E. dermatitidis is a slow-growing fungus, which usually takes up to two weeks to culture in the microbiology laboratory and therefore is slow to detect conventionally, with the risk of bacterial overgrowth from common co-habiting pan- and multiresistant bacterial pathogens from sputum. namely Pseudomonas aeruginosa and Burkholderia cepacia complex organisms, hence this species-specific PCR assay may help detect this organism from CF sputum more specifically and rapidly. Overall, employment of this novel assay nay help in the understanding of the occurrence. aetiology and epidemiology of E. dermatitidis, as an emerging fungal agent in patients with CF. (C) 2008 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.