995 resultados para 7140-220


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Background: The number of Escherichia coli in the gut of Crohn's disease (CD) patients is higher than that of normal subjects, but the virulence potential of these bacteria is not fully known. Previous studies have shown that these E. coli are closely related to extraintestinal pathogenic categories (ExPEC), are able to invade epithelial cells, and usually do not produce exotoxins. We report here the detection, in a CD patient, of an E. coli which belongs to a classical enteropathogenic (EPEC) serotype and displays virulence markers of enteroinvasive (EIEC), enteroaggregative (EAEC) and enterohemorrhagic (EHEC) pathotypes. Methods: The E. coli strain was isolated, in 2009, by classical bacteriological procedures from a 56 year old woman who underwent ileo-terminal resection 1 year before, due to intestinal obstruction. The bacterial characterization was carried out by in vitro adhesion and invasion assays to cultured epithelial cells and macrophages and screening by PCR to identify virulence genetic markers of diarrheogenic E. coli (DEC) and to detect one of the gene combinations which define the phylogroups of the E. coli reference (EcoR) collection. The strain was also tested for the ability to produce biofilm and shiga cytotoxins and had its whole genome sequenced by Ion Torrent Sequencing Technology. Results: The studied strain, which was detected both in ileum biopsies and the stools of the patient, displayed the aggregative adherence (AA) phenotype to Hep-2 cells and an ability to enter Caco-2 cells 3x as high as that of EIEC reference strain and 89% of that of the prototype AIEC LF82 strain. Although it could invade cultured macrophages, the strain was unable to replicate inside these cells. PCR screening revealed the presence of eae, aggR and stx1. Tests with bacterial culture supernatants in Vero cells demonstrating cytotoxicity suggested the production of Stx1. In addition, the strain revealed to be a strong biofilm producer, belonged to the B2 EcoR phylogroup, to the O126:H27 serogroup and to the multilocus sequencing type (MLST) ST3057. The 2 later features were deduced from the whole genome sequence of the strain. Conclusions: The characterization of this E. coli isolate from a CD patient revealed a combination of virulence markers of distinct DEC pathotypes, namely eae and stx1 of EHEC, AA, aggR and biofilm formation of EAEC, and invasiveness of EIEC. These features along with its serotype and phylogroup identity seem to suggest a potential to be involved in CD, an observation which should be tested with additional studies.

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The Rock Hill Hardware Company was organized on June 4, 1893 by A.R. Smith and John Gelzer, A.A. Barron and his sons R.E. and W.L. bought Smith out in 1896 and by 1907 had acquired the whole firm. The Barron family owned and operated it until it closed in 1978. The collection consists of a 1906 ledger, financial records, a photograph, a seed license, World War II ration booklets, and newspaper clippings.

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6 Briefe zwischen Max Horkheimer und F. Pollock mit Einbezug von Arthur G. Coons vom Occidental College Los Angeles, 1947; 4 Briefe zwischen Kathleen Costello und Max Horkheimer, 1945-1946; 8 Briefe und Beilage zwischen Edward M. David und Max Horkheimer, 24.11.1941-1942; 11 Briefe zwischen dem United States of America, Department of States und Max Horkheimer, 1942-1949; 3 Briefe zwischen Monroe E. Deutsch und Max Horkheimer, 1946; 12 Briefe und Beilage zwischen dem Dictionary of the Arts und Max Horkheimer, 1941-1944 sowie 1 Manuskrip: Sociology of Arts von Max Horkheimer; 2 Briefe zwischen Robert Disraeli und Max Horkheimer, 1945; 21 Briefe und Beilage zwischen Ria Drevermann und Max Horkheimer, 1948-1950; 6 Briefe zwischen Wolf Drewermann und Max Horkheimer, 1948-1949; 2 Briefe von Stephen Duggan vom Institute of International Education an Max Horkheimer, 1942-1947; 6 Briefe zwischen Joseph Dunner und Max Horkheimer, 1945-1947; 24 Briefe und Beilage zwischen Gretl Dupont und Max Horkheimer, 1942-1948; 3 Briefe zwischen Clarence A. Dykstra und Max Horkheimer, 1946-1947, 1947;

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u.a.: Suche nach einem Verleger; spanisches Zitat aus einer Ausgabe der "Historia de la literatura espanola"; Bekanntmachung eigener Übersetzung von Baltasar Graciáns "Criticon" ins Deutsche; Johann Gottlob von Quandt;

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76 Briefe zwischen Henryk Grossmann und Max Horkheimer, 1934 - 1949; 1 Brief von F. Pollock an K. H. Hennings, 13.07.1967; 2 Briefe zwischen Max Horkheimer und George Rainer, September 1950; 1 Brief von M. Kisling an Henryk Grossmann, 13.01.1950; 4 Briefe von Friedrich Pollock an Frederick Wild 1948 - 1949; 1 Brief von Henryk Grossmann an die Social Studies Association (New York), 05.05.1946; 1 Brief von Friedrich Pollock an die Social Studies Association (New York), 18.01.1949; 1 Brief von Max Horkheimer an den American Consul General (Habana), 26.04.1938; 1 Brief von Max Horkheimer an die New York Section of the National Council of Jewish Women (New York), 18.04.1938;

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Königstein, Keine Gefahr der Verhaftung

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130 Briefe zwischen Max Horkheimer und Leo Löwenthal, September 1946 - April 1947; 2 Briefe zwischen Herrn Klingelhöfer (Ministerualrat) und Felix J. Weil, 1946; 1 Brief von Max Horkheimer an Fritz Karsen, 21.11.1946; 1 Brief von Herbert Marcuse an Leo Löwenthal, 16.10.1946; 1 Brief von Max Horkheimer an Herbert Marcus, 28.10.1946; 4 Briefe zwischen Samuel H. Flowerman und Max Horkheimer, 1946; 1 Brief von Walter Hallstein an Felix J. Weil, 17.10.1946; 1 Brief von Heinz Sauermann an Felix J. Weil, 16.10.1946;

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Vorbesitzer: Frédéric Hillemacher