Organizational and direct support maintenance repair and special tools list : shop equipment, electrical repair, semitrailer mounted (southwest truck body model SER), serial no. range S-4-629 thru S-4-663 (NSN 4940-00-165-4020), serial no. range S-4-001 thur [sic] S-4-078 (NSN 4940-00-159-8847), serial no. range SER-1001 thur [sic] SER-1078 (NSN 4940-01-022-5322).

"April 1982."

Operator's, organizational and direct support maintenance manual : shop equipment, electrical repair, semi-trailer mounted : (Southwest Truck Body model SER), serial no. range S-4-629 thru S-4-663, NSN 4940-00-165-4020, serial no. range S-4001 thru S-4-078, NSN 4940-00-159-8847, serial no. range SER-1001 thru SER-1078, NSN 4940-01-022-5322.

Includes index.

Operator, organizational, direct support, and general support maintenance manual : semitrailer, stake: 12-ton, 4-wheel, M127 (2330-00-797-9207), M127A1 (2330-00-048-7743), M127A1C (2330-752-9750) and M127A2C (2330-00-788-6299), semitrailer, van: cargo, 12-ton, 4-wheel, M128A1 (2330-00-679-5581), M128A1C (2330-00-752-9751), and M128A2C (2330-00-788-6296), semitrailer, van: supply, 12-ton, 4-wheel, M129A1 (2330-00-629-1673), M129A1C (2330-00-752-9752), and M129A2C (2330-00-788-6289).

Includes index.

Operator's, organizational, DS, and GS maintenance manual (including repair parts and special tools lists) : trailer, air conditioner, 1 1/2 ton, 2-wheel, M463 (2330-629-5913).

"September 1971."

Organizational and direct support maintenance repair parts and special tools list (including depot maintenance repair parts and special tools) for viewers, driver's night vision, AN/VVS-2(V)1 (NSN 5855-00-629-5278), AN/VVS-2(V)2 (NSN 5855-01-057-1880), AN/VVS-2(V)3 (NSN 5855-01-105-7793), AN/VVS-2(V)1A (NSN 5855-01-096-0871), and AN/VVS-2(V)2A (NSN 5855-01-096-0872).

Includes index.

Organizational and direct support maintenance manual for viewer, drivers, night vision, AN/VVS-2(V)1 (NSN 5855-00-629-5278).

"March 1977."

Operator's manual : viewers, driver's, night vision, AN/VVS-2(V)1 (NSN 5855-00-629-5278), AN/VVS-2(V)1A (NSN 5855-01-096-0871), AN/VVS-2(V)2 (NSN 5855-01-057-1880), AN/VVS-2(V)2A (NSN 5855-01-096-0872), AN/VVS-2(V)3 (NSN 5855-01-105-7793).

"August 1982."

Operator's manual : night vision sight, individual served weapon AN/PVS-4 (5855-00-629-5334).

"13 September 1985."

Spaces of care in the third sector : Understanding the effects of professionalization

Increasingly the health and welfare needs of individuals and communities are being met by third sector, or not-for-profit, organizations. Since the 1980s third sector organizations have been subject to significant, sector-wide changes, such as the development of contractual funding and an increasing need to collaborate with governments and other sectors. In particular, the processes of ‘professionalization’ and ‘bureaucratization’ have received significant attention and are now well documented in third sector literature. These processes are often understood to create barriers between organizations and their community groups and neutralize alternative forms of service provision. In this article we provide a case study of an Australian third sector organization undergoing professionalization. The case study draws on ethnographic and qualitative interviews with staff and volunteers at a health-based third sector organization involved in service provision to marginalized community groups. We examine how professionalization alters organizational spaces and dynamics and conclude that professionalized third sector spaces may still be ‘community’ spaces where individuals may give and receive care and services. Moreover, we suggest that these community spaces hold potential for resisting the neutralizing effects of contracting.

Social scientists don’t say titwank’

Drawing on the textual evidence of a number of referees’ reports, this article maps key differences between the humanities and social sciences approaches to the study of pornography, in order to facilitate better understanding and communication between the areas. 1. Social scientists avoid ‘vulgar’ language to describe sex. Humanities scholars need not do so. 2. Social scientists remain committed to the idea of ‘objectivity’ while humanities scholars reject the idea – although this may be a confusion in language, with the term in the social sciences used to mean something more like ‘falsifiability’. 3. Social science assumes that the primary effects of exposure to pornography must be negative. 4. More generally, social science resists paradigm changes, insisting that all new work agrees with research that has gone before. 5. Social science believes that casual sex and sadomasochism are negative; humanities research need not do so.

Discovery and characterization of IGFBP-mediated endocytosis in the human retinal pigment epithelial cell line ARPE-19

Insulin-like growth factor binding proteins (IGFBPs) are prime regulators of IGF-action in numerous cell types including the retinal pigment epithelium (RPE). The RPE performs several functions essential for vision, including growth factor secretion and waste removal via a phagocytic process mediated in part by vitronectin (Vn). In the course of studying the effects of IGFBPs on IGF-mediated VEGF secretion and Vn-mediated phagocytosis in the RPE cell line ARPE-19, we have discovered that these cells avidly ingest synthetic microspheres (2.0 μm diameter) coated with IGFBPs. Given the novelty of this finding and the established role for endocytosis in mediating IGFBP actions in other cell types, we have explored the potential role of candidate cell surface receptors. Moreover, we have examined the role of key IGFBP structural motifs, by comparing responses to three members of the IGFBP family (IGFBP-3, IGFBP-4 and IGFBP-5) which display overlapping variations in primary structure and glycosylation status. Coating of microspheres (FluoSpheres®, sulfate modified polystyrene filled with a fluorophore) was conducted at 37 °C for 1 h using 20 μg/mL of test protein, followed by extensive washing. Binding of proteins was confirmed using a microBCA assay. The negative control consisted of microspheres treated with 0.1% bovine serum albumin (BSA), and all test samples were post-treated with BSA in an effort to coat any remaining free protein binding sites, which might otherwise encourage non-specific interactions with the cell surface. Serum-starved cultures of ARPE-19 cells were incubated with microspheres for 24 h, using a ratio of approximately 100 microspheres per cell. Uptake of microspheres was quantified using a fluorometer and was confirmed visually by confocal fluorescence microscopy. The ARPE-19 cells displayed little affinity for BSA-treated microspheres, but avidly ingested large quantities of those pre-treated with Vn (ANOVA; p < 0.001). Strong responses were also observed towards recombinant formulations of non-glycosylated IGFBP-3, glycosylated IGFBP-3 and glycosylated IGFBP-5 (all p < 0.001), while glycosylated IGFBP-4 induced a relatively minor response (p < 0.05). The response to IGFBP-3 was unaffected in the presence of excess soluble IGFBP-3, IGF-I or Vn. Likewise, soluble IGFBP-3 did not induce uptake of BSA-treated microspheres. Antibodies to either the transferrin receptor or type 1 IGF-receptor displayed slight inhibitory effects on responses to IGFBPs and Vn. Heparin abolished responses to Vn, IGFBP-5 and non-glycosylated IGFBP-3, but only partially inhibited the response to glycosylated IGFBP-3. Our results demonstrate for the first time IGFBP-mediated endocytosis in ARPE-19 cells and suggest roles for the IGFBP-heparin-binding domain and glycosylation status. These findings have important implications for understanding the mechanisms of IGFBP actions on the RPE, and in particular suggest a role for IGFBP-endocytosis.