Integrative functional genomics analysis of sustained polyploidy phenotypes in breast cancer cells identifies an oncogenic profile for GINS2

Aneuploidy is among the most obvious differences between normal and cancer cells. However, mechanisms contributing to development and maintenance of aneuploid cell growth are diverse and incompletely understood. Functional genomics analyses have shown that aneuploidy in cancer cells is correlated with diffuse gene expression signatures and that aneuploidy can arise by a variety of mechanisms, including cytokinesis failures, DNA endoreplication and possibly through polyploid intermediate states. Here, we used a novel cell spot microarray technique to identify genes with a loss-of-function effect inducing polyploidy and/or allowing maintenance of polyploid cell growth of breast cancer cells. Integrative genomics profiling of candidate genes highlighted GINS2 as a potential oncogene frequently overexpressed in clinical breast cancers as well as in several other cancer types. Multivariate analysis indicated GINS2 to be an independent prognostic factor for breast cancer outcome (p = 0.001). Suppression of GINS2 expression effectively inhibited breast cancer cell growth and induced polyploidy. In addition, protein level detection of nuclear GINS2 accurately distinguished actively proliferating cancer cells suggesting potential use as an operational biomarker.

Silva Fennica Vol. 23, 1989.

Julkaistu Silva Fennica Vol. 23(4) -numeron liitteenä.

Changing Paradigms in Biblical Criticism : 2 Kings 22:1 23:30 in the Flux of Discourses

The subject and methodology of biblical scholarship has expanded immense-ly during the last few decades. The traditional text-, literary-, source- and form-critical approaches, labeled historical-critical scholarship , have faced the challenge of social sciences. Various new literary, synchronic readings, sometimes characterized with the vague term postmodernism, have in turn challenged historicalcritical, and social-scientific approaches. Widened limits and diverging methodologies have caused a sense of crisis in biblical criticism. This metatheoretical thesis attempts to bridge the gap between philosophical discussion about the basis of biblical criticism and practical academic biblical scholarship. The study attempts to trace those epistemological changes that have produced the wealth of methods and results within biblical criticism. The account of the cult reform of King Josiah of Judah as reported in 2 Kings 22:1 23:30 serves as the case study because of its importance for critical study of the Hebrew Bible. Various scholarly approaches embracing 2 Kings 22:1 23:30 are experimentally arranged around four methodological positions: text, author, reader, and context. The heuristic model is a tentative application of Oliver Jahraus s model of four paradigms in literary theory. The study argues for six theses: 1) Our knowledge of the world is con-structed, fallible and theory-laden. 2) Methodological plurality is the neces-sary result of changes in epistemology and culture in general. 3) Oliver Jahraus s four methodological positions in regard to literature are also an applicable model within biblical criticism to comprehend the methodological plurality embracing the study of the Hebrew Bible. 4) Underlying the methodological discourse embracing biblical criticism is the epistemological ten-sion between the natural sciences and the humanities. 5) Biblical scholars should reconsider and analyze in detail concepts such as author and editor to overcome the dichotomy between the Göttingen and Cross schools. 6) To say something about the historicity of 2 Kings 22:1 23:30 one must bring together disparate elements from various disciplines and, finally, admit that though it may be possible to draw some permanent results, our conclusions often remain provisional.

Synthesis based on cyclohexadienes .23. Total synthesis of 5-epi-pupukean-2-one

A new strategy for the construction of the isotwistane skeleton is reported from easily available cyclohexadienes which involves stereoselective alkylation of a bicyclooctenone derivative and a decarboxylative 5-exo-trig radical cyclisation as the key steps in the total synthesis of 5-epi-pupukean-2-one.

Conformational Diversity in Contryphans from Conus Venom: cis-trans Isomerisation and Aromatic/Proline Interactions in the 23-Membered Ring of a 7-Residue Peptide Disulfide Loop

Conformational diversity or shapeshifting in cyclic peptide natural products can, in principle, confer a single molecular entity with the property of binding to multiple receptors. Conformational equilibria have been probed in the contryphans, which are peptides derived from Conus venom possessing a 23-membered cyclic disulfide moiety. The natural sequences derived from Conus inscriptus, GCV(D)LYPWC* (In936) and Conus loroisii, GCP(D)WDPWC* (Lo959) differ in the number of proline residues within the macrocyclic ring. Structural characterisation of distinct conformational states arising from cis-trans equilibria about Xxx-Pro bonds is reported. Isomerisation about the C2-P3 bond is observed in the case of Lo959 and about the Y5-P6 bond in In936. Evidence is presented for as many as four distinct species in the case of the synthetic analogue V3P In936. The Tyr-Pro-Trp segment in In936 is characterised by distinct sidechain orientations as a consequence of aromatic/proline interactions as evidenced by specific sidechain-sidechain nuclear Overhauser effects and ring current shifted proton chemical shifts. Molecular dynamics simulations suggest that Tyr5 and Trp7 sidechain conformations are correlated and depend on the geometry of the Xxx-Pro bond. Thermodynamic parameters are derived for the cis trans equilibrium for In936. Studies on synthetic analogues provide insights into the role of sequence effects in modulating isomerisation about Xxx-Pro bonds.

Prudentia Iuris, 1990, n° 23 (número completo)

Contenido: Doctor Santiago de Estrada / Alfredo Di Pietro – Jornadas sobre “La reforma del Estado”: Las alternativas de financiamiento del mercado de capitales / Joaquín R. Ledesma – Una reflexión sobre la ley de reforma del Estado / Carlos Raúl Sanz – Las privatizaciones / Juan Carlos Cassagne – La concesión de obra pública / Rodolfo Barra – Constitucionalidad de las privatizaciones / Néstor Pedro Sagüés – Aspectos laborales de la ley 23.696 / Hugo R. Carcavallo – Estado nacional y bien común / José Giménez Rébora – Atribuciones, conducta y control de los funcionarios públicos en la época hispánica / Julio R. Lascano – Notas acerca de las relaciones Iglesia-Estado / Néstor Daniel Villa – Interdictos y acciones populares en Roma/ Fernando M. Zamora – Ciencias políticas: Ortega y la “sociedad de naciones” / Enrique Aguilar -- Documentos

Letras, 1990-1991, nº 23-24 (número completo)

Contenido: Un pequeño gran gigante. Más notas acerca de la hipérbole de lo corporal en la obra de Rabelais / Susana G. Artal – Una lectura de los cuatro cuartetos de T. S. Eliot (segunda parte) / Inés de Cassagne – Los estudios lingüísticos con base computacional / María Amalia García Jurado – Bucolismo en una carta de Sidonio Apolinar / Raúl Lavalle – La seducción estética de la barbarie como poesía de lo original originario / María Rosa Lojo – La elegía a María Blanchard / Lía Noemí Uriarte Rebaudi – Nota – Reseñas bibliográficas

Estado forestal del Pinus caribaea Morelet var. Hondurensis en el Pinal, Laguna de Perlas, RAAS

Tbe present study was carried out in the strip of land coast between Pearls Lagoon community and the Caribbean sea, in the nearness of Pinar lagoon, 25 km to the north of Bluefields. The geographical coordinates of the area are 12" 13' N and 83" 42' west. Tbe climate presents an annua! rninfall of 4 250 mm, an avera· ge temperature of 26 "C and a relative humidity of 89 %. The topography is plain and tbe elevation oscillates between O· 10 masl. The ma.in objective of the study was to evaluate the forest conditions in which forest of P. Caribaea var .. Jwndurei'ISis ealled the Pinal are found. The methodology employed consisted of gathering all information using air pllotographs and topograpbieal maps to defined the area and stands group (designed A, B, C, D, E, F and G). Two block and seven stands were found, in which invent.ory lines with sample plots of 500 m1 each one were employed to measure the indivíduals with DBH over 10 cm. The intensily it shows ís of !5% and was measured diameter, height, age and other. In tbis forest there where found seven stands with a total area covered with pine of 312.42 has. The average age is 23 years and estirnated total volume of 97.4 ms of wood. The density is of 60,61 trees hectare, with an average volume of 13.02 m3 /has and a commercial volume of 8.29 m' 1 has. Where found lndices of place 12 (stands G), 9 y 6. Besides, the 6.3.64 % of the trees has a satisfactory tendency of growlh (quality l and 2). lt can be concluded that the resource is in badly state due to intensive exploitations and annual f!fCS; is a young forest (23 years old), dotninated by índi viduals of small diameters (10-25 cm) and medium height (5-25 m); the available areas or with possibilities of being planting are: estimated in 468.64 has. This forest has a half annual increment of 1.4.3 cm/year, which is considered excellent and is c1assified with Clas P-III, FAO Classification by forests stratification.

南海北部湾23/25太阳区块海底工程地质调查与评价

<正> 本项成果,是承包美国太阳东方勘探公司任务而完成的。在系统分析总结了南海西部石油公司“南海—503工程地质取芯船”在南海北部湾23/25太阳区块所进行的测深、侧扫声纳、地震声学剖面测量,及4口孔深40米的工程地质浅钻土柱等材料的基础上,详细阐述了