A Safe Vaccine (DV-STM-07) against Salmonella Infection Prevents Abortion and Confers Protective Immunity to the Pregnant and New Born Mice

Pregnancy is a transient immuno-compromised condition which has evolved to avoid the immune rejection of the fetus by the maternal immune system. The altered immune response of the pregnant female leads to increased susceptibility to invading pathogens, resulting in abortion and congenital defects of the fetus and a subnormal response to vaccination. Active vaccination during pregnancy may lead to abortion induced by heightened cell mediated immune response. In this study, we have administered the highly attenuated vaccine strain delta pmrG-HM-D (DV-STM-07) in female mice before the onset of pregnancy and followed the immune reaction against challenge with virulent S. Typhimurium in pregnant mice. Here we demonstrate that DV-STM-07 vaccine gives protection against Salmonella in pregnant mice and also prevents Salmonella induced abortion. This protection is conferred by directing the immune response towards Th2 activation and Th1 suppression. The low Th1 response prevents abortion. The use of live attenuated vaccine just before pregnancy carries the risk of transmission to the fetus. We have shown that this vaccine is safe as the vaccine strain is quickly eliminated from the mother and is not transmitted to the fetus. This vaccine also confers immunity to the new born mice of vaccinated mothers. Since there is no evidence of the vaccine candidate reaching the new born mice, we hypothesize that it may be due to trans-colostral transfer of protective anti-Salmonella antibodies. These results suggest that our vaccine DV-STM-07 can be very useful in preventing abortion in the pregnant individuals and confer immunity to the new born. Since there are no such vaccine candidates which can be given to the new born and to the pregnant women, this vaccine holds a very bright future to combat Salmonella induced pregnancy loss.

Photo induced optical changes in Sb/As2S3 multilayered film and (As2S3)(0.93)Sb-0.07 film of equal thickness

The increase in optical band gap (photo bleaching) due to light illumination was studied at room temperature as well as at low (4.2 K) temperature for Sb/As2S3 multilayered film of 640 nm thickness by Fourier Transform Infrared Technique. The interdiffusion of Sb into As2S3 matrix results the formation of Sb-As2S3 ternary solid solutions which is explained by the change in optical band gap (E-g), absorption coefficient (alpha), Tauc parameter (B-1/2), Urbach edge (E-e). At the same time, photo darkening phenomena was observed in (As2S3)(0.93)Sb-0.07 film of same thickness both at low and room temperatures. From our X-ray Photoelectron Spectroscopy measurements,we are able to show that some of the As-As, S-S and Sb-Sb bonds are converted into As-S and S-Sb bonds in case of multilayers. We found that the energetically favoured heteropolar bond formation take place by a phonon-assisted mechanism using the lone pair pi electrons of S-2(0). But in case of (As2S3)(0.93)Sb-0.02 film, the homopolar bonds are playing a major role. (C) 2010 Elsevier B.V. All rights reserved.

Synthesis dependent characteristics of Sr1-xMnxTiO3 (x=0.03, 0.05, 0.07 and 0.09)

Sr1-xMnxTiO3 (where x=0.03, 0.05, 0.07 and 0.09) was synthesized via different routes that include solid-state, oxalate precipitation and freeze drying. In oxalate precipitation technique, compositions corresponding to 3 and 5 mol% doping of Mn were monophasic whereas the higher compositions revealed the presence of the secondary phases such as MnO, Mn3O4 etc., as confirmed by high resolution X-ray diffraction (XRD) studies. The decomposition behavior of the precursors prepared using oxalate precipitation method corresponding to the above mentioned compositions was studied. Nanopowders of compositions pertaining to 5 to 9 mol% of Mn doping were obtained using freeze-drying technique. The average crystallite size of these nanopowders was found to be in the 35 to 65 nm range. The microstructural studies carried out on the sintered ceramics, fabricated using powders synthesized by different routes established the fine grained nature ( < 1 mu m) of the one obtained by freeze drying method. Raman scattering studies were carried out in order to complement the observations made from XRD regarding the phase purity. The dielectric properties of the ceramics obtained by different synthesis routes were studied in the 80-300 K temperature range at 100 kHz and the effect of grain size has been discussed. (C) 2012 Elsevier Inc. All rights reserved.

Under-representation of intrinsic terminators across bacterial genomic islands: Rho as a principal regulator of xenogenic DNA expression

Two transcription termination mechanisms - intrinsic and Rho-dependent - have evolved in bacteria. The Rho factor occurs in most bacterial lineages, and has been hypothesized to play a global regulatory role. Genome-wide studies using microarray, 2D-gel electrophoresis and ChIP-chip provided evidence that Rho serves to silence transcription from horizontally acquired genes and prophages in Escherichia coli K-12, implicating the factor to be a part of the ``cellular immune mechanism'' protecting against deleterious phages and aberrant gene expression from acquired xenogenic DNA. We have investigated this model by adopting an alternate in silico approach and have extended the study to other species. Our analysis shows that several genomic islands across diverse phyla have under-representation of intrinsic terminators, similar to that experimentally observed in E. coli K-12. This implies that Rho-dependent termination is the predominant process operational in these islands and that silencing of foreign DNA is a conserved function of Rho. From the present analysis, it is evident that horizontally acquired islands have lost intrinsic terminators to facilitate Rho-dependent termination. These results underscore the importance of Rho as a conserved, genome-wide sentinel that regulates potentially toxic xenogenic DNA. (C) 2012 Elsevier B.V. All rights reserved.

Memory effect in SrRu(1-x)O3 (0.01 < x < 0.07)

SrRuO3 is widely known to be an itinerant ferromagnet with a T-C similar to 160 K. It is well known that glassy materials exhibit time dependent phenomena such as memory effect due to their generic slow dynamics. However, for the first time, we have observed memory effect in SrRu(1-x)O3 (0.01

Biodegradation of acrylamide by acrylamidase from Stenotrophomonas acidaminiphila MSU12 and analysis of degradation products by MALDI-TOF and HPLC

The present study examines an improved detoxification and rapid biological degradation of toxic pollutant acrylamide using a bacterium. The acrylamide degrading bacterium was isolated from the soil followed by its screening to know the acrylamide degrading capability. The minimal medium containing acrylamide (30 mM) served as a sole source of carbon and nitrogen for their active growth. The optimization of three different factors was analyzed by using Response Surface Methodology (RSM). The bacteria actively degraded the acrylamide at a temperature of 32 degrees C, with a maximum growth at 30 mM substrate (acrylamide) concentration at a pH of 7.2. The acrylamidase activity and degradation of acrylamide was determined by High Performance Liquid Chromatography (HPLC) and Matrix Assisted Laser Desorption and Ionization Time of Flight mass spectrometer (MALDI-TOF). Based on 168 rRNA analysis the selected strain was identified as Gram negative bacilli Stenotrophomonas acidaminiphila MSU12. The acrylamidase was isolated from bacterial extract and was purified by HPLC, whose mass spectrum showed a molecular mass of 38 kDa. (C) 2014 Elsevier Ltd. All rights reserved.

Non-enzymatic electronic detection of glucose using aminophenylboronic acid functionalized reduced graphene oxide

We report the non-enzymatic electronic detection of glucose using field effect transistor (FET) devices made of aminophenylboronic acid (APBA) functionalized reduced graphene oxide (RGO). Detection of glucose molecules was carried out over a wide dynamic range of concentration varying from 100 pM to 100 mM with a detection limit of similar to 2 nM using both covalently and non-covalently functionalized APBA-RGO complex. The normalized change in electrical conductance data shows that the FET devices made of non-covalently functionalized APBA-RGO complex (nc-APBA-RGO) exhibited a linear response to glucose aqueous solution of concentrations varying from 1 nM to 10 mM and showed 4 times enhanced sensitivity over the devices made of covalently functionalized APBA-RGO complex (c-APBA-RGO). Specificity of APBA-RGO complex to glucose is confirmed from the observation of negligible change in electrical conductance after exposure to 0.1 mM of lactose and other interfering factors. (C) 2015 Elsevier B.V. All rights reserved.

Identificación macroscópica de patologías hepáticas de mayor prevalencia en bovinos faenados en el matadero PROINCASA, período del 07 de julio del 2010 - enero del 2011

El presente estudio se llevó a cabo para determinar la prevalencia de las patologías macroscópicas en hígados decomisados (condenados) de bovinos faenados en el matadero PROINCASA, identificando las patologías hepáticas mediante la inspección, clasificando los tipos de enfermedades (primarias o secundarias) según las patologías identificadas y determinando las pérdidas económicas representadas por los hígados decomisados. Los datos recopilados se obtuvieron a través de la inspección post mortem de los órganos, en los que se realizó la debida observación, palpación, limpieza y laminado de los mismos. Se utilizó estadística descriptiva con distribuciones de frecuencias a partir de los datos recolectados, se estructuró la base de datos en hoja electrónica Microsoft Excel℗, se realizó un análisis de los datos mediante la prueba de Chi2 (X2), utilizando el programa estadístico SPSS℗, generando tablas de contingencia a una P <0.05. Las variables evaluadas fueron: prevalencia total de patologías, prevalencia de patologías específicas, y pérdidas económicas; con los datos recopilados, se encontró desde el punto de vista general, que el mes con mayor prevalencia de hígados afectados fue septiembre con un 16%, y el mes con menor prevalencia fue julio con 12%; haciendo un análisis individual por patología, el mes con mayor prevalencia de hígados afectados con abscesos fue julio con un 5%, telangiectasia en octubre con un 9%, Ictericia en agosto con un 5% y para adherencia fue octubre con un 8%, del total de hígados con diferentes patologías por mes se obtuvo la mayor prevalencia en octubre con un 56% para abscesos, telangiectasia en octubre con un 70%, ictericia en septiembre con un 26% y adherencia en octubre con un 27%. De un total de 11 286 hígados inspeccionados, 1 555 resultaron afectados, lo que equivale a un 13.78% de afección, a su vez estos representan económicamente la pérdida de C$ 466,500 Córdobas netos.

Prudentia Iuris, 1982, n° 07 (número completo)

Contenido: Editorial – Jorge Joaquín Llambías / Mario Amadeo – El aporte de Llambías en materia de efectos de la nulidad de los actos jurídicos / Roberto Martínez Ruíz – El matrimonio en la regulación propuesta por el anteproyecto de 1954 / Jorge A. Mazzinghi – La reparación del agravio moral / Guillermo R. Quintana Terán – Responsabilidad civil de las personas jurídicas y, en especial, del estado / Federico N. Videla Escalada – La mora en las obligaciones cuyo lugar de pago es el domicilio del deudor / Francisco Alberto Vocos – Algunas páginas de Llambías – Documentos – Notas bibliográficas

Sobre as alterações no crédito imobiliário e outras referentes à política habitacional contidas na MP 2.212 de 30/8/01 e nas MPs 2.221 e 2.223 de 4/9/01

Consultoria Legislativa - Área VII - Finanças, Direito Comercial, Direito Econômico, Defesa do Consumidor - Área XI - Meio Ambiente, Geografia, Urbanismo, Arquitetura.

[Discurso do Deputado Áureo Melo : Sessão da Câmara dos Deputados de 07 de maio de 1964]

Aborda a campanha de certos jornais do Rio de Janeiro contra Brasília. Destaca o verdadeiro pensamento dos cariocas relativamente ao pretendido retorno da capital da República para a Guanabara.

[Discurso do Deputado Múcio Athayde : Sessão da Câmara dos Deputados de 07 de maio de 1985]

Aborda a necessidade de representação política, em todos os níveis, para o Distrito Federal e da implantação de Câmara de Vereadores em cada cidade satélite. Faz críticas à Comissão Interpartidária encarregada da efetivação das reformas políticas e eleitorais.