杜仲抗真菌蛋白晶体生长的原子力显微成像研究——快速生长与晶面生长速率

杜仲抗真菌蛋白(Eucommiaantifungalprotein,EAFP)的单晶体具有在几小时内就可长大的快速生长特性.用原子力显微成像(atomicforcemicroscope,AFM)技术,原位实时观测了EAFP单斜晶体生长过程中的｛10 0｝表面形貌动态变化,并分别在不同的过饱和度下测量了其生长速率.结果表明,EAFP晶体生长的速率与蛋白质溶液的过饱和度相关,在过饱和度高时(σ =1 78)晶面生长极快;在中等过饱和度(σ =1 5)下,其晶面台阶的生长速率沿b,c方向分别为 12nm/s和 2 4 2nm/s,比溶菌酶生长速率(6～ 7nm/s)快很多;在蛋白质浓度很低的情况下,其生长速率仍与其他蛋白质相当.EAFP晶体快速生长可能与该分子尺寸较小,内部结构紧凑,分子骨架呈刚性和分子表面性质等其固有特性密切相关.沉淀剂浓度对EAFP晶体生长也有影响.过饱和度很低时,提高沉淀剂浓度会干扰晶体生长.

Propagation of Explosion Wave in Hard-Soft-Hard Layered Media

The paper presents a reasonable analysis for dynamic response and failure process of a plane multi-layered media, which are subjected to a blast loading. This blast loading is induced by a cylindric explosive put on the center of top surface of the layered media. With the help of numerical simulation technique provided by LS-DYNA software, the whole process of explosion wave propagation and attenuation can be revealed. The feature of local failure around the blasting site is also discussed in some detail. Our focus will be on the explosion wave attenuation for the hard-soft-hard sandwich layers. As seen in the paper, the computational results are delivered in a feasible way by comparing with experimental data.