Use of isozyme patterns in the identification of Biomphalaria tenagophila (D'Orbigny, 1835) and B. occidentalis (Paraense, 1981) (Gastropoda: Planorbidae)

Two sibling species of Biomphalaria, B. tenagophila and B. occidentalis were identified using isozyme patterns obtained by horizontal gel electrophoresis. Six diagnostic enzymatic loci were identified in digestive gland homogenates. The results enable us to distinguish the species, calculate the Nei's coefficient of genetic similarity, and provide a basis for making inferences about the pattern of these two planorbid species colonization and distribution.

Neotropical planorbid snails with apertural lamellae: I. Biomphalaria helophila (Orbigny, 1835)

A definition of Biomphalaria helophila (Orbigny, 1835) is presented, based on examination of the shell and reproductive system of topotypic specimens and extended to a number of samples from other localities. The following nominal species and subspecies, collected from type localities, proved junior synonyms of B. helophila: Planorbis albicans Pfeiffer, 1839; Planorbis dentatus Gould, 1844; Planorbis dentiferus CB Adams, 1845; Planorbis dentiferus edentatus CB Adams, 1851; Planorbis dentiens Morelet, 1849; Planorbula dentiens edentula Fischer & Crosse, 1880; Planorbis stagnicola Morelet, 1851; and Tropicorbis shimeki FC Baker, 1945. B. helophila was also identified in samples from Costa Rica, Guatemala, Haiti, Dominican Republic, Puerto Rico and Barbados.

Description of Pythonella scleruri n. sp. (Apicomplexa: Eimeriidae) from a Brazilian Bird Rufous-Breasted-Leaftosser Sclerurus scansor, 1835 (Passeriformes: Furnariidae)

Coccidian oocysts containing 16 sporocysts with 4 sporozoites in each were observed in a faecal sample from Sclerurus scansor collected in the Itatiaia National Park, southeastern region of Brazil. The oocysts are characterized by ellipsoidal shape measuring 42.5 x 32.8 mm, with smooth, thick double-layered wall of a greenish-orange colour. An oocyst residuum of numerous scattered granules among the sporocysts in sporulated ones; 16 round sporocysts, averaging 10.5 x 10 mm each containing four elongated sporozoites; presence of residuum; absence of Stieda body. The presently described coccidian, recorded for the first time in birds, is a new species named P. scleruri.

Effects of the desiccation on Biomphalaria tenagophila (Orbigny, 1835) (Mollusca) infected by Schistosoma mansoni Sambon, 1907

Specimens of Biomphalaria tenagophila exposed to miracidia of Schistosoma mansoni were submitted to different desiccation periods as follows: group I: 24 h after exposure, desiccated for 28 days; group II: after cercariae elimination, desiccated for 7 days; group III: 21 days after exposure, desiccated for 7 days; group IV: 14 days after exposure, desiccated for 14 days; group V: 7 days after exposure, desiccated for 21 days. From the obtained data it was verified that desiccation was not capable of interrupting the development of larvae of S. mansoni in mollusks. A delay in the development of S. mansoni larvae in groups I, III, IV and V was observed. A pause was verified in the development of S. mansoni larvae in groups II, III, IV and V. Some larvae, in groups I, III, IV and V, did not suffer as a result of desiccation and continued their development. Larvae in the cercariae stage were shown to be more sensitive to desiccation. It was possible to obtain clearing of mollusks infected by sporocysts II and cercariae using a period of 7 days of desiccation.

Molluscicidal activity of Physalis angulata L. extracts and fractions on Biomphalaria tenagophila (d'Orbigny, 1835) under laboratory conditions

The main objective of this research is to evaluate the molluscicide activity of Physalis angulata L. Biomphalaria tenagophila specimens under laboratory conditions. Extracts and fractions were supplied by the Laboratório de Química de Produtos Naturais, Farmanguinhos-Fiocruz. Experiments were performed according to the methodology described by the World Health Organization for molluscicide tests using the concentrations from 0.1 to 500 mg/l of the extracts, fractions and of a pool of physalins modified steroids present in this species. The results show that ethyl acetate and acetone extracts from the whole plant, the ethanolic extracts of the roots and the physalins pool from stems and leaves were active. Only the whole plant extracts were available in sufficient quantity for the determination of LD50 and LD90 values.

Southernmost finding of Lymnaea viatrix orbigny, 1835 (Pulmonata: Lymnaeidae), intermediate host of Fasciola hepatica (Linnaeus, 1758) (Trematoda: Digenea), in urban and rural areas of Patagonia, Argentina

We report the first finding of Lymnaea viatrix south of parallel 41ºS, in rural and urban areas from Argentina. Ninety snails were collected during year 2000, from a concrete pond at a Public Square in El Bolsón Village, Río Negro province, and 811 snails in November 1999, and during 2000 from waterbodies within a farm at Cholila locality, Chubut province. Fasciola hepatica infection was detected in 0.9% snails from the rural area. We discuss the potential risk of L. viatrix to public health in urban areas and its epidemiological importance in rural areas of the Andean Patagonian region.

Consens i revolució: Poble i nació a la Barcelona de la Revolució Liberal, 1835-1843

Anàlisi dels deferents discursos de moderats, progressistes i republicans i els usos dels mots “poble” i “nació” durant els anys 1837-1843. L’autor considera que aquesta perspectiva és important per entendre el període, tot i que els recents debats sobre els orígens del nacionalisme a Espanya ho han deixat en un lloc secundari

Phylogenetic analysis of Biomphalaria tenagophila (Orbigny, 1835) (Mollusca: Gastropoda)

Mitochondrial DNA of Biomphalaria tenagophila, a mollusc intermediate host of Schistosoma mansoni in Brazil, was sequenced and characterised. The genome size found for B. tenagophila was 13,722 bp and contained 13 messenger RNAs, 22 transfer RNAs (tRNA) and two ribosomal RNAs (rRNA). In addition to sequencing, the mitochondrial DNA (mtDNA) genome organization of B. tenagophila was analysed based on its content and localization of both coding and non-coding regions, regions of gene overlap and tRNA nucleotide sequences. Sequences of protein, rRNA 12S and rRNA 16S nucleotides as well as gene organization were compared between B. tenagophila and Biomphalaria glabrata, as the latter is the most important S. mansoni intermediate host in Brazil. Differences between such species were observed regarding rRNA composition. The complete sequence of the B. tenagophila mitochondrial genome was deposited in GenBank (accession EF433576). Furthermore, phylogenetic relationships were estimated among 28 mollusc species, which had their complete mitochondrial genome deposited in GenBank, using the neighbour-joining method, maximum parsimony and maximum likelihood bootstrap. B. tenagophila was positioned at a branch close to B. glabrata and Pulmonata molluscs, collectively comprising a paraphyletic group, contrary to Opistobranchia, which was positioned at a single branch and constituted a monophyletic group.

Sobre Gymnocerus Audinet-Serville, 1835 (Coleoptera, Cerambycidae, Lamiinae, Anisocerini) e gêneros relacionados

On Gymnocerus Audinet-Serville, 1835 (Coleoptera, Cerambycidae, Lamiinae, Anisocerini) and related genera. The genus Gymnocerus Audinet-Serville, 1835 is characterized and a key to the related genera is added. New combinations: Gymnocerina cratosomoides (Bates, 1862), comb. nov. (from Gymnocerus) and Badenella gavisa (Lane, 1966), comb. nov. (from Chimboincola). New synonyms proposed: Badenella Lane, 1964 = Chimboincola Lane, 1966 syn. nov.; Gymnocerina cratosomoides (Bates, 1862) = Gymnocerus crassus Bates, 1862 syn. nov. = Gymnocerina sulfurea Lane, 1964 syn. nov. = Gymnocerina subsiciva Lane, 1964 syn. nov. = Gymnocerina spuria Lane, 1964 syn. nov. = Gymnocerina oliveirai Lane, 1964 syn. nov.; and Badenella badeni (Bates, 1875) = Gymnocerus badeni fuscus Franz, 1935 syn. nov. = Badenella laceyi Lane, 1964 syn. nov..

Uma nova espécie de Cladomorphus Gray, 1835 (Phasmatidae, Cladomorphinae) de Minas Gerais, Brasil

Cladomorphus trimariensis sp. nov. é descrita de Três Marias, Minas Gerais. A nova espécie difere de C. phyllinus Gray, 1835 principalmente pelo opérculo e placa micropilar do ovo, ausência de espinhos nas pernas médias e posteriores e pelas projeções distais nos tergos IV e V, que são menos desenvolvidas.

Aspectos biológico - Pesqueros del “calamar común”, Loligo gahi d’Orbigny, 1835 en el área del Callao durante 1996 y 1997

El Calamar común, Loligo gahi (Cephalopoda: Loliginidae) es una especie comercialmente importante en la pesquería artesanal de la costa peruana. Este estudio tiene como objetivo determinar aspectos biológicos de esta especie y su relación con la estacionalidad de la pesquería. Los especímenes se obtuvieron a partir de los desembarques de la pesquería artesanal del puerto de Callao en 1996-1997. Fue hallada la estructura de tallas de los individuos muestreados y su relación longitud - peso. La variación de diferentes índices reproductivos durante 1996 y 1997 se analizaron a fin de determinar el pico de desove. La estimación de la edad se hizo usando tanto datos de frecuencia de tallas, de este mismo período, como del conteo de anillos de crecimiento en estatolitos. Se detallan los aspectos mas significantes de la pesquería artesanal de L. gahi en Callao y se presenta la variación del CPUE en 1996. Los resultados sugieren que el ciclo de vida de L. gahi es parecido al que se describió en las Islas Malvinas (Falkland). El pico de desove se determinó en la primavera, con picos secundarios en el otoño e invierno. El modelo de crecimiento que mejor se ajusta a los datos es una función potencial y el tiempo de vida es aproximadamente de un año. Las fechas de eclosión fueron retrocalculadas en los meses de primavera, verano y otoño. La variación del Indice Gonadosomático (IGS) observada durante 1996 mostró los mismos picos pero uno o dos meses antes.