Synergistic inflammatory signaling in airway epithelial cells : control of expression levels of protease activated receptors and interleukin-8 release

Protease-activated receptor, interleukin, thrombin, trypsin, asthma

Sylloge monumentorum, ad illustrandam historiam Fennicam pertinentium 8

Painovuosi nimekkeestä.

Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells

Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.

Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells

Hydrogen Sulfide (H(2)S) a volatile Sulfur compound, is implicated as a cause of inflammation. especially when it is produced by bacteria colonizing gastrointestinal organs However, It IS Unclear if H(2)S produced by periodontal pathogens affects the inflammatory responses mediated by oral/gingival epithelial cells Therefore. the aims of this Study were (1) to compare the in vitro production of H(2)S among. 14 strains of Oral bacteria and (2) to evaluate the effects of H(2)S on inflammatory response induced in host oral/gingival epithelial cells Porphyromonas gingivalis (Pg) produced the most H(2)S in Culture, Which, in turn resulted in the promotion of proinflammatory cytokine IL-8 from both gingival and Oral epithelial cells The up-regulation of IL-8 expression was reproduced by the exogenously applied H(2)S Furthermore. the Mutant Strains of Pg that do not produce major Soluble Virulent factors. ie gingival, still showed the Production of H(2)S. as well as the promotion of epithelial IL-8 production. which was abrogated by H(2)S scavenging reagents These results demonstrated that Pg produces a concentration of H(2)S capable of Up-regulating-IL-8 expression induced in gingival and oral epithelial cells, revealing a possible mechanism that may promote the inflammation in periodontal disease (C) 2009 Elsevier B.V. All rights reserved

A prática pedagógica e a construção de saberes do biólogo no ensino de física na 8ª série

O trabalho em foco foi realizado com professores licenciados em Biologia que lecionam Física na disciplina Ciências Naturais. Apresenta alguns uestionamentos em torno do trabalho pedagógico desenvolvido por estes profissionais da educação para superar as dificuldades encontradas ao lecionarem aquela disciplina. Para subsidiar este estudo, analisei o currículo de licenciatura plena em Biologia de duas instituições de ensino superior, os erros conceituais nos livros didáticos e as relações que se estabelecem entre estes instrumentos, os Parâmetros Curriculares Nacionais e os saberes docentes do professor de Ciências. Os subsídios teóricos que nortearam a investigação estão centrados nos pensamentos de Paulo Freire e sua prática libertadora de educar, na escola reflexiva de Isabel Alarcão, nas competências de Perrenoud, entre outros autores que fazem reflexões pistemológicas sobre a prática pedagógica, e Orlandi, que trata das análises do Discurso. Para compreender melhor o trabalho docente desenvolvido pelos professores sujeitos desta pesquisa, analiso por meio de observações, entrevistas e questionários como se constroem os saberes docentes na atuação deste profissional, haja vista que ele não é formado para o exercício pedagógico na disciplina de Física. Os dados encontrados nas vozes desses professores mostram neste modelo de pesquisa o quanto é importante saber ouvir, para sentir e avaliar as esperanças, as angústias e outros problemas que interferem ou auxiliam na prática educativa. O estudo revela alguns elementos que servem para analisar como os professores constroem e estruturam suas práticas pedagógicas, sejam elas reflexivas ou não, como estruturam seus pensamentos em relação ao ensino de Ciências, e quais as alternativas possíveis para uma melhoria na qualidade desta prática. Revelam também que os livros didáticos são instrumentos que sustentam a prática dos professores, sobretudo para os que insistem em ficar presos ao currículo estabelecido pelo sistema educacional, ou ao currículo da formação inicial. Concluo, finalmente, que muito precisa ser feito para melhorar a alfabetização científica docente, mas apesar disso, encontramos professores comprometidos com a própria formação profissional. A construção dos saberes docentes não estão centradas apenas no currículo de formação profissional, ou na leitura dos livros didáticos, nem tampouco nas estratégias pedagógicas, porém incluem-se a esses saberes a construção dos conhecimentos experimentados ou vividos ao longo de sua vida, aos saberes construídos na realidade social da qual participa o docente, da realidade do sistema escolar, do saber da experiência, enfim a construção se faz com todos os “arranjos” que a sociedade expõe o docente/educador acompanhado de reflexões crítico-analíticas.

Comparison between known propagation models using least squares tuning algorithm on 5.8 GHz in Amazon region cities

ABSTRACT: This paper presents a performance comparison between known propagation Models through least squares tuning algorithm for 5.8 GHz frequency band. The studied environment is based on the 12 cities located in Amazon Region. After adjustments and simulations, SUI Model showed the smaller RMS error and standard deviation when compared with COST231-Hata and ECC-33 models.

The Amplifier - v.8,(a-6) no. 6

In this issue...Billings Gazette, Engineering Days, Valentine Dance, Sylvia White, speed skating, handball tournament, Women's News, Shell Oil Company, KXLF-TV

Search for Scalar Diphoton Resonances in the Mass Range 65-600 GeV with the ATLAS Detector in pp Collision Data at √s = 8 TeV

A search for scalar particles decaying via narrow resonances into two photons in the mass range 65–600 GeV is performed using 20.3  fb −1 of s √ =8  TeV pp collision data collected with the ATLAS detector at the Large Hadron Collider. The recently discovered Higgs boson is treated as a background. No significant evidence for an additional signal is observed. The results are presented as limits at the 95% confidence level on the production cross section of a scalar boson times branching ratio into two photons, in a fiducial volume where the reconstruction efficiency is approximately independent of the event topology. The upper limits set extend over a considerably wider mass range than previous searches.

Evidence for Electroweak Production of W±W±jj in pp Collisions at √s=8 TeV with the ATLAS Detector

This Letter presents the first study of W ± W ± jj , same-electric-charge diboson production in association with two jets, using 20.3  fb −1 of proton-proton collision data at s √ =8  TeV recorded by the ATLAS detector at the Large Hadron Collider. Events with two reconstructed same-charge leptons (e ± e ± , e ± μ ± , and μ ± μ ± ) and two or more jets are analyzed. Production cross sections are measured in two fiducial regions, with different sensitivities to the electroweak and strong production mechanisms. First evidence for W ± W ± jj production and electroweak-only W ± W ± jj production is observed with a significance of 4.5 and 3.6 standard deviations, respectively. The measured production cross sections are in agreement with standard model predictions. Limits at 95% confidence level are set on anomalous quartic gauge couplings

Chlorine isotope composition in chlorofluorocarbons CFC-11, CFC-12 and CFC-113 in firn, stratospheric and tropospheric air

The stratospheric degradation of chlorofluorocarbons (CFCs) releases chlorine, which is a major contributor to the destruction of stratospheric ozone (O3). A recent study reported strong chlorine isotope fractionation during the breakdown of the most abundant CFC (CFC-12, CCl2F2, Laube et al., 2010a), similar to effects seen in nitrous oxide (N2O). Using air archives to obtain a long-term record of chlorine isotope ratios in CFCs could help to identify and quantify their sources and sinks. We analyse the three most abundant CFCs and show that CFC-11 (CCl3F) and CFC-113 (CClF2CCl2F) exhibit significant stratospheric chlorine isotope fractionation, in common with CFC-12. The apparent isotope fractionation (ϵapp) for mid- and high-latitude stratospheric samples are respectively −2.4 (0.5) and −2.3 (0.4) ‰ for CFC-11, −12.2 (1.6) and −6.8 (0.8) ‰ for CFC-12 and −3.5 (1.5) and −3.3 (1.2) ‰ for CFC-113, where the number in parentheses is the numerical value of the standard uncertainty expressed in per mil. Assuming a constant isotope composition of emissions, we calculate the expected trends in the tropospheric isotope signature of these gases based on their stratospheric 37Cl enrichment and stratosphere–troposphere exchange. We compare these projections to the long-term δ (37Cl) trends of all three CFCs, measured on background tropospheric samples from the Cape Grim air archive (Tasmania, 1978–2010) and tropospheric firn air samples from Greenland (North Greenland Eemian Ice Drilling (NEEM) site) and Antarctica (Fletcher Promontory site). From 1970 to the present day, projected trends agree with tropospheric measurements, suggesting that within analytical uncertainties, a constant average emission isotope delta (δ) is a compatible scenario. The measurement uncertainty is too high to determine whether the average emission isotope δ has been affected by changes in CFC manufacturing processes or not. Our study increases the suite of trace gases amenable to direct isotope ratio measurements in small air volumes (approximately 200 mL), using a single-detector gas chromatography–mass spectrometry (GC–MS) system.