Investigation of the Epitaxial Graphene/p-SiC Heterojunction

There has been significant research in the study of in-plane charge-carrier transport in graphene in order to understand and exploit its unique electrical properties; however, the vertical graphene–semiconductor system also presents opportunities for unique devices. In this letter, we investigate the epitaxial graphene/p-type 4H-SiC system to better understand this vertical heterojunction. The I–V behavior does not demonstrate thermionic emission properties that are indicative of a Schottky barrier but rather demonstrates characteristics of a semiconductor heterojunction. This is confirmed by the fitting of the temperature-dependent I–V curves to classical heterojunction equations and the observation of band-edge electroluminescence in SiC.

Desarrollo de un sistema de almacenamiento térmico para la central termosolar Astexol 2

En este proyecto se elabora la ingeniería básica de un sistema de almacenamiento térmico para la central termosolar Astexol 2 (Badajoz, España). Para ello, primeramente se decidió que el sistema térmico de almacenamiento a diseñar fuera indirecto de dos tanques con sales fundidas. Una vez seleccionado el tipo de almacenamiento adecuado, se seleccionó la capacidad de almacenamiento óptima, en base a unos objetivos establecidos para la instalación del TES en Astexol 2. Finalmente, se procedió con el diseño de los equipos principales y con la realización de los planos de implantación y de los PFD’s (diagramas de flujo) y P&ID’s (diagramas de instrumentación y control) del sistema de almacenamiento. ABSTRACT This project includes the basic engineering of a thermal storage system for the concentrating solar power plant Astexol 2 (Badajoz, Spain). First of all, it was decided that the type of thermal storage system to design had to be an indirect two-tank molten salt thermal storage system. Once the proper type of storage system was chosen, the optimum storage capacity was selected, according to the main aims designated for the thermal storage system installation in Astexol 2.Finally, the design of the main equipment of the thermal storage system was done, together with the elaboration of the associated plot plans, PFD’s (Process Flow Diagrams) and P&ID’s (Process and Instrumentation Diagrams).

Construcción modular ligera energéticamente eficiente

Esta tesis trata sobre la construcción modular ligera, dentro del contexto de la eficiencia energética y de cara a los conceptos de nZEB (near Zero Energy Building) y NZEB (Net Zero Energy Building) que se manejan en el ámbito europeo y específicamente dentro del marco regulador de la Directiva 2010/31 UE. En el contexto de la Unión Europea, el sector de la edificación representa el 40% del total del consumo energético del continente. Asumiendo la necesidad de reducir este consumo se han planteado, desde los organismos de dirección europeos, unos objetivos (objetivos 20-20-20) para hacer más eficiente el parque edificatorio. Estos objetivos, que son vinculantes en términos de legislación, comprometen a todos los estados miembros a conseguir la meta de reducción de consumo y emisiones de GEI (Gases de Efecto Invernadero) antes del año 2020. Estos conceptos de construcción modular ligera (CML) y eficiencia energética no suelen estar asociados por el hecho de que este tipo de construcción no suele estar destinada a un uso intensivo y no cuenta con unos cerramientos con niveles de aislamiento de acuerdo a las normativas locales o códigos de edificación de cada país. El objetivo de nZEB o NZEB, e incluso Energy Plus, según sea el caso, necesariamente (y así queda establecido en las normativas), dependerá no sólo de la mejora de los niveles de aislamiento de los edificios, sino también de la implementación de sistemas de generación renovables, independientemente del tipo de sistema constructivo con el que se trabaje e incluso de la tipología edificatoria. Si bien es cierto que los niveles de industrialización de la sociedad tecnológica actual han alcanzado varias de las fases del proceso constructivo - sobre todo en cuanto a elementos compositivos de los edificios- también lo es el hecho de que las cotas de desarrollo conseguidas en el ámbito de la construcción no llegan al nivel de evolución que se puede apreciar en otros campos de las ingenierías como la aeronáutica o la industria del automóvil. Aunque desde finales del siglo pasado existen modelos y proyectos testimoniales de construcción industrializada ligera (CIL) e incluso ya a principios del siglo XX, ejemplos de construcción modular ligera (CML), como la Casa Voisin, la industrialización de la construcción de edificios no ha sido una constante progresiva con un nivel de comercialización equiparable al de la construcción masiva y pesada. Los términos construcción industrializada, construcción prefabricada, construcción modular y construcción ligera, no siempre hacen referencia a lo mismo y no siempre son sinónimos entre sí. Un edificio puede ser prefabricado y no ser modular ni ligero y tal es el caso, por poner un ejemplo, de la construcción con paneles de hormigón prefabricado. Lo que sí es una constante es que en el caso de la construcción modular ligera, la prefabricación y la industrialización, casi siempre vienen implícitas en muchos ejemplos históricos y actuales. Con relación al concepto de eficiencia energética (nZEB o incluso NZEB), el mismo no suele estar ligado a la construcción modular ligera y/o ligera industrializada; más bien se le ve unido a la idea de cerramientos masivos con gran inercia térmica propios de estándares de diseño como el Passivhaus; y aunque comúnmente a la construcción ligera se le asocian otros conceptos que le restan valor (corta vida útil; función y formas limitadas, fuera de todo orden estético; limitación en los niveles de confort, etc.), los avances que se van alcanzando en materia de tecnologías para el aprovechamiento de la energía y sistemas de generación renovables, pueden conseguir revertir estas ideas y unificar el criterio de eficiencia + construcción modular ligera. Prototipos y proyectos académicos– como el concurso Solar Decathlon que se celebra desde el año 2002 promovido por el DOE (Departamento de Energía de los Estados Unidos), y que cuenta con ediciones europeas como las de los años 2010 y 2012, replantean la idea de la construcción industrializada, modular y ligera dentro del contexto de la eficiencia energética, con prototipos de viviendas de ± 60m2, propuestos por las universidades concursantes, y cuyo objetivo es alcanzar y/o desarrollar el concepto de NZEB (Net Zero Energy Building) o edificio de energía cero. Esta opción constructiva no sólo representa durabilidad, seguridad y estética, sino también, rapidez en la fabricación y montaje, además de altas prestaciones energéticas como se ha podido demostrar en las sucesivas ediciones del Solar Decathlon. Este tipo de iniciativas de desarrollo de tecnologías constructivas, no sólo apuntan a la eficiencia energética sino al concepto global de energía neta, Energía plus o cero emisiones de CO2. El nivel de emisiones por la fabricación y puesta en obra de los materiales de construcción depende, en muchos casos, no solo de la propia naturaleza del material, sino también de la cantidad de recursos utilizados para producir una unidad de medida determinada (kg, m3, m2, ml, etc). En este sentido podría utilizarse, en muchos casos, el argumento válido de que a menos peso, y a menos tamaño, menos emisiones globales de gases de efecto invernadero y menos contaminación. Para el trabajo de investigación de esta tesis se han tomado como referencias válidas para estudio, prototipos tanto de CML (Modular 3D) como de CIL (panelizado y elementos 2D), dado que para los fines de análisis de las prestaciones energéticas de los materiales de cerramiento, ambos sistemas son equiparables. Para poder llegar a la conclusión fundamental de este trabajo de tesis doctoral - que consiste en demostrar la viabilidad tecnológica/ industrial que supone la combinación de la eficiencia energética y la construcción modular ligera - se parte del estudio del estado de la técnica ( desde la selección de los materiales y los posibles procesos de industrialización en fábrica, hasta su puesta en obra, funcionamiento y uso, bajo los conceptos de consumo cero, cero emisiones de carbono y plus energético). Además -y con un estado de la técnica que identifica la situación actual- se llevan a cabo pruebas y ensayos con un prototipo a escala natural y células de ensayo, para comprobar el comportamiento de los elementos compositivos de los mismos, frente a unas condicionantes climáticas determinadas. Este tipo de resultados se contrastan con los obtenidos mediante simulaciones informáticas basadas en los mismos parámetros y realizadas en su mayoría mediante métodos simplificados de cálculos, validados por los organismos competentes en materia de eficiencia energética en la edificación en España y de acuerdo a la normativa vigente. ABSTRACT This thesis discusses lightweight modular construction within the context of energy efficiency in nZEB (near Zero Energy Building) and NZEB (Net Zero Energy Building) both used in Europe and, specifically, within the limits of the regulatory framework of the EU Directive 2010/31. In the European Union the building sector represents 40% of the total energy consumption of the continent. Due to the need to reduce this consumption, European decision-making institutions have proposed aims (20-20-20 aims) to render building equipment more efficient. These aims are bound by law and oblige all member States to endeavour to reduce consumption and GEI emissions before the year 2020. Lightweight modular construction concepts and energy efficiency are not generally associated because this type of building is not normally meant for intensive use and does not have closures with insulation levels which fit the local regulations or building codes of each country. The objective of nZEB or NZEB and even Energy Plus, depending on each case, will necessarily be associated (as established in the guidelines) not only with the improvement of insulation levels in buildings, but also with the implementation of renewable systems of generation, independent of the type of building system used and of the building typology. Although it is true that the levels of industrialisation in the technological society today have reached several of the building process phases - particularly in the composite elements of buildings - it is also true that the quotas of development achieved in the area of construction have not reached the evolutionary levelfound in other fields of engineering, such as aeronautics or the automobile industry. Although there have been models and testimonial projects of lightweight industrialised building since the end of last century, even going back as far as the beginning of the XX century with examples of lightweight modular construction such as the Voisin House, industrialisation in the building industry has not been constant nor is its comercialisation comparable to massive and heavy construction. The terms industrialised building, prefabricated building, modular building and lightweight building, do not always refer to the same thing and they are not always synonymous. A building can be prefabricated yet not be modular or lightweight. To give an example, this is the case of building with prefabricated concrete panels. What is constant is that, in the case of lightweight modular construction, prefabrication and industrialisation are almost always implicit in many historical and contemporary examples. Energy efficiency (nZEB or even NZEB) is not normally linked to lightweight modular construction and/or industrialised lightweight; rather, it is united to the idea of massive closureswith high thermal inertia typical of design standards such as the Passive House; and although other concepts that subtract value from it are generally associated with lightweight building (short useful life, limited forms and function, inappropriate toany aesthetic pattern; limitation in comfort levels, etc.), the advances being achieved in technology for benefitting from energy and renewable systems of generation may well reverse these ideas and unify the criteria of efficiency + lightweight modular construction. Academic prototypes and projects - such as the Solar Decathlon competition organised by the US Department of Energy and celebrated since 2002, with its corresponding European events such as those held in 2010 and 2012, place a different slant on the idea of industrialised, modular and lightweight building within the context of energy efficiency, with prototypes of homes measuring approximately 60m2, proposed by university competitors, whose aim is to reach and/or develop the NZEB concept, or the zero energy building. This building option does not only signify durability, security and aesthetics, but also fast manufacture and assembly. It also has high energy benefits, as has been demonstrated in successive events of the Solar Decathlon. This type of initiative for the development of building technologies, does not only aim at energy efficiency, but also at the global concept of net energy, Energy Plus and zero CO2 emissions. The level of emissions in the manufacture and introduction of building materials in many cases depends not only on the inherent nature of the material, but also on the quantity of resources used to produce a specific unit of measurement (kg, m3, m2, ml, etc.). Thus in many cases itcould be validly arguedthat with less weight and smaller size, there will be fewer global emissions of greenhouse effect gases and less contamination. For the research carried out in this thesis prototypes such as the CML (3D Module) and CIL (panelled and elements) have been used as valid study references, becauseboth systems are comparablefor the purpose of analysing the energy benefits of closure materials. So as to reach a basic conclusion in this doctoral thesis - that sets out to demonstrate the technological/industrial viability of the combination of energy efficiency and lightweight modular construction - the departure point is the study of the state of the technique (from the selection of materials and the possible processes of industrialisation in manufacture, to their use on site, functioning and use, respecting the concepts of zero consumption, zero emissions of carbon and Energy Plus). Moreover, with the state of the technique identifying the current situation, tests and practices have been carried out with a natural scale prototype and test cells so as to verify the behaviour of the composite elements of these in certain climatic conditions. These types of result are contrasted with those obtained through computer simulation based on the same parameters and done, principally, using simplified methods of calculation, validated by institutions competent in energy efficiency in Spanish building and in line with the rules in force.

The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1

Recently, Salmonella spp. were shown to induce apoptosis in infected macrophages. The mechanism responsible for this process is unknown. In this report, we establish that the Inv-Spa type III secretion apparatus target invasin SipB is necessary and sufficient for the induction of apoptosis. Purified SipB microinjected into macrophages led to cell death. Binding studies show that SipB associates with the proapoptotic protease caspase-1. This interaction results in the activation of caspase-1, as seen in its proteolytic maturation and the processing of its substrate interleukin-1β. Caspase-1 activity is essential for the cytotoxicity. Functional inhibition of caspase-1 activity by acetyl-Tyr-Val-Ala-Asp-chloromethyl ketone blocks macrophage cytotoxicity, and macrophages lacking caspase-1 are not susceptible to Salmonella-induced apoptosis. Taken together, the data demonstrate that SipB functions as an analog of the Shigella invasin IpaB.

Targeted inactivation of sister of P-glycoprotein gene (spgp) in mice results in nonprogressive but persistent intrahepatic cholestasis

Mutations in the sister of P-glycoprotein (Spgp) or bile salt export pump (BSEP) are associated with Progressive Familial Intrahepatic Cholestasis (PFIC2). Spgp is predominantly expressed in the canalicular membranes of liver. Consistent with in vitro evidence demonstrating the involvement of Spgp in bile salt transport, PFIC2 patients secrete less than 1% of biliary bile salts compared with normal infants. The disease rapidly progresses to hepatic failure requiring liver transplantation before adolescence. In this study, we show that the knockout of spgp gene in mice results in intrahepatic cholestasis, but with significantly less severity than PFIC2 in humans. Some unexpected characteristics are observed. Notably, although the secretion of cholic acid in mutant mice is greatly reduced (6% of wild-type), total bile salt output in mutant mice is about 30% of wild-type. Also, secretion of an unexpectedly large amount of tetra-hydroxylated bile acids (not detected in wild-type) is observed. These results suggest that hydroxylation and an alternative canalicular transport mechanism for bile acids compensate for the absence of Spgp function and protect the mutant mice from severe cholestatic damage. In addition, the spgp−/− mice display a significant increase in the secretion of cholesterol and phospholipids into the bile. This latter observation in spgp−/− mice suggests that intrahepatic, rather than intracanalicular, bile salts are the major driving force for the biliary lipid secretion. The spgp−/− mice thus provide a unique model for gaining new insights into therapeutic intervention for intrahepatic cholestasis and understanding mechanisms associated with lipid homeostasis.

Defective localization of the NADPH phagocyte oxidase to Salmonella-containing phagosomes in tumor necrosis factor p55 receptor-deficient macrophages

Tumor necrosis factor receptor (TNFR) p55-knockout (KO) mice are susceptible profoundly to Salmonella infection. One day after peritoneal inoculation, TNFR-KO mice harbor 1,000-fold more bacteria in liver and spleen than wild-type mice despite the formation of well organized granulomas. Macrophages from TNFR-KO mice produce abundant quantities of reactive oxygen and nitrogen species in response to Salmonella but nevertheless exhibit poor bactericidal activity. Treatment with IFN-γ enhances killing by wild-type macrophages but does not restore the killing defect of TNFR-KO cells. Bactericidal activity of macrophages can be abrogated by a deletion in the gene encoding TNFα but not by saturating concentrations of TNF-soluble receptor, suggesting that intracellular TNFα can regulate killing of Salmonella by macrophages. Peritoneal macrophages from TNFR-KO mice fail to localize NADPH oxidase-containing vesicles to Salmonella-containing vacuoles. A TNFR-KO mutation substantially restores virulence to an attenuated mutant bacterial strain lacking the type III secretory system encoded by Salmonella pathogenicity island 2 (SPI2), suggesting that TNFα and SPI2 have opposing actions on a common pathway of vesicular trafficking. TNFα–TNFRp55 signaling plays a critical role in the immediate innate immune response to an intracellular pathogen by optimizing the delivery of toxic reactive oxygen species to the phagosome.

Identificação e caracterização de RNA mensageiros candidatos a alvo das proteínas PUMILHO de Arabidopsis através do sistema triplo-híbrido de levedura

Proteínas PUF regulam a estabilidade e a tradução através da ligação a seqüências específicas nas regiões 3\' não traduzidas (3\' UTR) dos mensageiros. A ligação é mediada por um domínio de ligação conservado constituído por 8 repetições de aproximadamente 36 aminoácidos cada. Experimentos realizados no sistema triplo-híbrido de levedura mostraram que os homólogos PUF de Arabidopsis APUM-1, APUM-2 e APUM-3 são capazes de ligar especificamente à seqüência chamada de Elemento de Resposta a NANOS (NRE) reconhecida pelo homólogo PUF de Drosophila. A utilização de bibliotecas de expressão de RNA em ensaios no sistema triplo-híbrido permitiu a identificação de seqüências de ligação consenso para as três proteínas APUM. Análises computacionais identificaram elementos de ligação a APUM em regiões 3\' UTR de importantes transcritos relacionados ao controle do meristema do caule e à manutenção das células totipotentes. Nós mostramos que os homólogos APUM-l, APUM-2 e APUM-3 reconhecem elementos de ligação a APUM nas regiões 3\' UTR dos transcritos WUSCHEL, CLAVATA-1, ZWILLE e FASCIATA-2. Ensaios de RT-PCR e Western blot semiquantitativos mostraram que a quantidade dos transcritos WUSHEL e CLAVATA-1 é alterada em plantas antisenso induzíveis para APUM-l, APUM-2 e APUM-3. A relevância biológica dessas interações foi observada através de ensaios de coimunoprecipitação, confirmando, portanto, o primeiro caso de regulação traducional descrito para os mensageiros WUSCHEL e CLAVATA-1. Análises computacionais adicionais para a identificação de outros homólogos PUF em Arabidopsis encontraram vinte e cinco proteínas possuindo repetições PUF. Entre elas, os homólogos APUM-4, APUM-S e APUM-6 apresentam alta similaridade com as proteínas APUM-l, APUM-2 e APUM-3, sendo capazes de ligar especificamente à seqüência NRE e aos elementos de ligação a APUM presentes nas regiões 3\' UTR dos transcritos WUSCHEL, CLAVATA-1, ZWILLE e FASCIATA-ts resultados indicam que vários homólogos PUF podem agir como reguladores traducionais em Arabidopsis através de um mecanismo molecular conservado entre as espécies, podendo abrir uma nova área de investigação da regulação de mRNA em plantas.

An introduction to alysidal algebra (V): phenomenological components

Purpose – This paper aims to refer to a subjective approach to a type of complex system: human ecosystems, referred to as deontical impure systems (DIS) to capture a set of properties fundamental to the distinction between human and natural ecosystems. There are four main phenomenological components: directionality, intensity, connection energy and volume. The paper establishes thermodynamics of deontical systems based on the Law of Zipf and the temperature of information. Design/methodology/approach – Mathematical and logical development of human society structure. Findings – A fundamental question in this approach to DIS is the intensity or forces of a relation. Concepts are introduced as the system volume and propose a system thermodynamic theory. It hints at the possibility of adapting the fractal theory by introducing the fractal dimension of the system. Originality/value – This paper is a continuation of other previous papers and developing the theory of DIS.

Evolution of Outcrossing in Experimental Populations of Caenorhabditis elegans

Caenorhabditis elegans can reproduce exclusively by self-fertilization. Yet, males can be maintained in laboratory populations, a phenomenon that continues to puzzle biologists. In this study we evaluated the role of males in facilitating adaptation to novel environments. For this, we contrasted the evolution of a fitness component exclusive to outcrossing in experimental populations of different mating systems. We introgressed a modifier of outcrossing into a hybrid population derived from several wild isolates to transform the wild-type androdioecious mating system into a dioecious mating system. By genotyping 375 single-nucleotide polymorphisms we show that the two populations had similar standing genetic diversity available for adaptation, despite the occurrence of selection during their derivation. We then performed replicated experimental evolution under the two mating systems from starting conditions of either high or low levels of diversity, under defined environmental conditions of discrete non-overlapping generations, constant density at high population sizes (N = 10(4)), no obvious spatial structure and abundant food resources. During 100 generations measurements of sex ratios and male competitive performance showed: 1) adaptation to the novel environment; 2) directional selection on male frequency under androdioecy; 3) optimal outcrossing rates of 0.5 under androdioecy; 4) the existence of initial inbreeding depression; and finally 5) that the strength of directional selection on male competitive performance does not depend on male frequencies. Taken together, these results suggest that androdioecious males are maintained at intermediate frequencies because outcrossing is adaptive.